BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,678,164	(T)_8→7	pseudogene (157/367 nt)	mdtJ ←	multidrug/spermidine efflux SMR transporter subunit MdtJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,678,157	0	T	.	100.0%	3122.2 / NA	645	pseudogene (164/367 nt)	mdtJ	multidrug/spermidine efflux SMR transporter subunit MdtJ
Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (352/293); total (352/293)

Only 100 of 677 total aligned reads displayed.

CACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGATTTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCA  >  NZ_CP025268/1678020‑1678292

      ccgggCGACCAGGGTGGTTAACCCGGATATTTTCATCAGCTATAAAATTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACATTCCCACAGCGCATAAGCTACGCCTAAGGCGAtttttttaa                                                                                                                                 <  1:9468422/137‑3 (MQ=255)

      cccGGCGACCCGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGACTAACAAAACGCTAAACAAGGTAAGAAATAAAATACCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGAtttttttaa                                                                                                                                 <  2:9562789/140‑3 (MQ=255)

          gCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGc                                                                                                                            >  1:7927034/1‑140 (MQ=255)

          gCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGc                                                                                                                            >  1:1737958/1‑140 (MQ=255)

           cGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCg                                                                                                                           <  1:7767942/140‑1 (MQ=255)

               cAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAag                                                                                                                       >  2:5427893/1‑140 (MQ=255)

                  ggtggtTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAagaga                                                                                                                    >  1:6469187/1‑140 (MQ=255)

                  ggtggtTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAagaga                                                                                                                    <  1:1604407/140‑1 (MQ=255)

                   gtggtTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAagagag                                                                                                                   <  2:11254631/140‑1 (MQ=255)

                     gtttAACCCGGCAATTTACATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGA‑TTTTTATAACGGCGAAAGAGAGaa                                                                                                                 <  2:6928528/138‑1 (MQ=255)

                     ggtTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGaa                                                                                                                 <  2:5780997/140‑1 (MQ=255)

                     ggtTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGaa                                                                                                                 <  2:10760607/140‑1 (MQ=255)

                     ggtTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGaa                                                                                                                 <  2:3475352/140‑1 (MQ=255)

                     ggtTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAAGACCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGaa                                                                                                                 <  2:4055638/140‑1 (MQ=255)

                       ttAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAat                                                                                                               <  2:2303983/140‑1 (MQ=255)

                       ttAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAat                                                                                                               >  1:7164862/1‑140 (MQ=255)

                           cccGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAatatat                                                                                                           >  2:1208515/1‑140 (MQ=255)

                           cccGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAatatat                                                                                                           >  2:10163426/1‑140 (MQ=255)

                            ccGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAAtatata                                                                                                          >  1:6838104/1‑140 (MQ=255)

                                cAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGag                                                                                                      >  1:10402677/1‑139 (MQ=255)

                                cAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGAc                                                                                                      >  2:5965778/1‑140 (MQ=255)

                                            gCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAatcatc                                                                                          >  2:4869441/1‑140 (MQ=255)

                                             cGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAAtcatca                                                                                         >  1:2195101/1‑140 (MQ=255)

                                               aTAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCAcc                                                                                       <  1:8794458/140‑1 (MQ=255)

                                               aTAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCAcc                                                                                       <  1:322102/140‑1 (MQ=255)

                                                       tcgtcgAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTaa                                                                               >  1:1004918/1‑140 (MQ=255)

                                                       tcgtcgAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTaa                                                                               >  2:272155/1‑140 (MQ=255)

                                                             aaTAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGaa                                                                                                                 >  2:11411072/1‑100 (MQ=255)

                                                              aTAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAg                                                                        >  1:4833885/1‑140 (MQ=255)

                                                               tAACAAAACGCTAAACAAGGTAATAAATAAAAGACCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAgc                                                                       <  1:3989804/140‑1 (MQ=255)

                                                                   aaaaCGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAgccgcc                                                                   <  1:7301149/140‑1 (MQ=255)

                                                                   aaaaCGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAgccgcc                                                                   <  2:2067542/140‑1 (MQ=255)

                                                                     aaCGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGAGCATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCAGCACCAGCATTAAAATAAAGCCGCCAt                                                                 >  1:3313742/1‑140 (MQ=255)

                                                                       cGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGaaa                                                                                                                        <  1:429653/83‑1 (MQ=255)

                                                                         cTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTcc                                                             <  2:4506291/140‑1 (MQ=255)

                                                                            aaCAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGACATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTc                                                          >  2:2505356/1‑140 (MQ=255)

                                                                              cAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCAc                                                        <  2:8486735/140‑1 (MQ=255)

                                                                                 ggTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGa                                                     >  1:5720173/1‑140 (MQ=255)

                                                                                 ggTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGa                                                     >  1:1943952/1‑140 (MQ=255)

                                                                                      taaataaaATACCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCa                                                                                   <  1:11050363/105‑1 (MQ=255)

                                                                                        aataaaataCCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGTGTAATATATACGACAGAGAAATCATCACCTGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGc                                              <  1:2931915/140‑1 (MQ=255)

                                                                                        aataaaataCCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGc                                              <  2:329313/140‑1 (MQ=255)

                                                                                          taaaataCCGATACCTTCCCACCGCGCATAAGCTACGCCTTATGAGA‑TTTTTTTAACGGCGAAAGAGAGATATATATACGACAGAGAAATCATCAACAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGccc                                            <  2:8033238/140‑1 (MQ=255)

                                                                                          taaaataCCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGccc                                            <  1:3106039/140‑1 (MQ=255)

                                                                                          taaaataCCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGccc                                            <  1:1313751/140‑1 (MQ=255)

                                                                                          taaaataCCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGccc                                            <  1:5249359/140‑1 (MQ=255)

                                                                                          taaaataCCGATACCTTCCCACAGCGCAAAAGCAACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGACAGAAATAAATACGACAGAGAAAACATCACCAGCATTAAAATAAAGCCGCCATTTCCCACACTGACGCTCGccc                                            <  2:881190/140‑1 (MQ=255)

                                                                                          taaaataACGATACCTTCCCACAGCTCATAAGCTACGCCTAAGGCGA‑TTTTTTTAACTGCGAAAGAGAGAAATATATACGACAGATAAATCATCACCAGAATTAAAATAAAGCCTCCATTTCCCTCACTGACGCTCGccc                                            <  2:7030773/140‑1 (MQ=255)

                                                                                          gaccataCCGATACCTTCCCACAGCGCAGAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATCTATCCGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCCCGGACGCTCGccc                                            <  2:313765/136‑1 (MQ=255)

                                                                                           aaaataCCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAagagag                                                                                                                   <  2:6283716/68‑1 (MQ=255)

                                                                                           aaaataCCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAagagag                                                                                                                   >  1:6283717/1‑68 (MQ=255)

                                                                                            aaataCCGATACCTTCCCACAGCGCATACGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGCCCAt                                          <  1:2687944/140‑1 (MQ=255)

                                                                                             aataCCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGCCCAtt                                         >  1:10584967/1‑140 (MQ=255)

                                                                                             aataCCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGCCCAtt                                         >  1:11382440/1‑140 (MQ=255)

                                                                                               taCCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCAt                                                                                  <  2:731072/97‑1 (MQ=255)

                                                                                                  cGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTc                                                              >  2:167660/1‑114 (MQ=255)

                                                                                                    aTACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGCCCATTTCATTGa                                  >  2:10616797/1‑140 (MQ=255)

                                                                                                    aTACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGCCCATTTCATTGa                                  >  2:9232997/1‑140 (MQ=255)

                                                                                                          tCCCACAGCGCATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGt                            >  2:3636323/1‑140 (MQ=255)

                                                                                                          tCCCACAGCGCATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGCCCAATTCATTGACAGCGt                            >  2:10073057/1‑140 (MQ=255)

                                                                                                                  cgcATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACCGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGAACCGGTaa                    >  2:5485456/1‑140 (MQ=255)

                                                                                                                  cgcATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTaa                    >  2:10454280/1‑140 (MQ=255)

                                                                                                                  cgcATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTaa                    >  1:9846241/1‑140 (MQ=255)

                                                                                                                  cgcATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTaa                    >  2:6379422/1‑140 (MQ=255)

                                                                                                                  cgcATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTaa                    >  2:790703/1‑140 (MQ=255)

                                                                                                                  cgcATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTaa                    >  2:822518/1‑140 (MQ=255)

                                                                                                                   gcATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAAt                   <  1:2516597/140‑1 (MQ=255)

                                                                                                                    cATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAAtt                  >  2:5639708/1‑140 (MQ=255)

                                                                                                                    cATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAAtt                  >  2:2875007/1‑140 (MQ=255)

                                                                                                                    cATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAAtt                  >  2:6550841/1‑140 (MQ=255)

                                                                                                                    cATAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAAtt                  >  1:4342234/1‑140 (MQ=255)

                                                                                                                     aTAAGCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAAttt                 >  2:871270/1‑140 (MQ=255)

                                                                                                                         gCTACGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGt             >  1:8107268/1‑140 (MQ=255)

                                                                                                                            aCGCCTAAGGCTA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGc          <  2:3848229/140‑1 (MQ=255)

                                                                                                                            aCGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACTACAGAGAAATCAACACCAGCATTAAAATAAAGCAGCCATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGc          >  1:8667030/1‑140 (MQ=255)

                                                                                                                            aCGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGc                                              <  2:9693023/104‑1 (MQ=255)

                                                                                                                            aCGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGc          >  1:282953/1‑140 (MQ=255)

                                                                                                                            aCGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGc          >  1:3496068/1‑140 (MQ=255)

                                                                                                                            aCGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGc          <  1:9047542/140‑1 (MQ=255)

                                                                                                                            aCGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGc          >  2:7690661/1‑140 (MQ=255)

                                                                                                                            aCGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCAATTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGc          >  2:11055949/1‑140 (MQ=255)

                                                                                                                            aCGCCTAAGGCGA‑TATTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGc          <  2:1609503/140‑1 (MQ=255)

                                                                                                                             cgaatAAGGCGA‑TTTTTTTAACGTCTAAAGAGAGAAATATATACGACAGAGAAATCATCAACAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCa         <  2:1883649/136‑1 (MQ=255)

                                                                                                                             cGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGATAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCa         <  2:1480822/140‑1 (MQ=255)

                                                                                                                             cGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCa         <  1:5965779/140‑1 (MQ=255)

                                                                                                                             cGCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCa         <  1:10305107/140‑1 (MQ=255)

                                                                                                                              gCCTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCaa        >  1:7542890/1‑140 (MQ=255)

                                                                                                                               ccTAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAAt       >  2:5235315/1‑140 (MQ=255)

                                                                                                                                 tAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAg     <  1:4963342/140‑1 (MQ=255)

                                                                                                                                 tAAGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAg     >  1:9724363/1‑140 (MQ=255)

                                                                                                                                   aGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCAcc                                                                                       >  1:8966522/1‑56 (MQ=255)

                                                                                                                                   aGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCAcc                                                                                       <  2:8966521/56‑1 (MQ=255)

                                                                                                                                   aGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGcc   <  1:8389743/140‑1 (MQ=255)

                                                                                                                                   aGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGcc   >  1:5499324/1‑140 (MQ=255)

                                                                                                                                   aGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGcc   <  2:433716/140‑1 (MQ=255)

                                                                                                                                   aGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGcc   >  1:118781/1‑140 (MQ=255)

                                                                                                                                   aGGCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGcc   >  1:1073840/1‑140 (MQ=255)

                                                                                                                                    ggCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCa  >  2:4675350/1‑140 (MQ=255)

                                                                                                                                    ggCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCa  >  1:4677279/1‑140 (MQ=255)

                                                                                                                                    ggCGA‑TTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCa  >  1:3835192/1‑140 (MQ=255)

CACAATCCCGGCGACCAGGGTGGTTAACCCGGCAATTTTCATCAGCGATAAACTTTCGTCGAATAACAAAACGCTAAACAAGGTAATAAATAAAATACCGATACCTTCCCACAGCGCATAAGCTACGCCTAAGGCGATTTTTTTTAACGGCGAAAGAGAGAAATATATACGACAGAGAAATCATCACCAGCATTAAAATAAAGCCGCCATTTCCCTCACTGACGCTCGCCCATTTCATTGACAGCGTACCGGTAATTTCTGTAGCAATAGCCA  >  NZ_CP025268/1678020‑1678292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`