BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,728,808	(C)_10→8	pseudogene (1254‑1255/1457 nt)	xylB ←	xylulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,728,800	0	C	.	89.5%	417.0 / 50.1	117	pseudogene (1263/1457 nt)	xylB	xylulokinase
Reads supporting (aligned to +/- strand): ref base C (12/0); new base . (52/50); total (65/52)
Fisher's exact test for biased strand distribution p-value = 1.05e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	NZ_CP025268	3,728,801	0	C	.	88.5%	325.9 / 80.0	117	pseudogene (1262/1457 nt)	xylB	xylulokinase
Reads supporting (aligned to +/- strand): ref base C (0/3); major base . (44/48); minor base T (12/0); total (65/52)
Fisher's exact test for biased strand distribution p-value = 3.63e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 138 total aligned reads displayed.

GGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCA  >  NZ_CP025268/3728661‑3728933

||

ggCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATc                                                                                                                                       <  2:11182452/140‑1 (MQ=255)

ggCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATc                                                                                                                                       <  2:2341569/140‑1 (MQ=255)

ggCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATc                                                                                                                                       <  1:1260145/140‑1 (MQ=255)

  cTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGTTTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccc                                                                                                                                     <  2:2691225/140‑1 (MQ=255)

  cTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccc                                                                                                                                     <  2:3350683/140‑1 (MQ=255)

  cTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccc                                                                                                                                     <  2:3541208/140‑1 (MQ=255)

  cTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccc                                                                                                                                     <  2:3540758/140‑1 (MQ=255)

   tGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATcccc                                                                                                                                    >  1:1500748/1‑140 (MQ=255)

       aaGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAGgcgccccc                                                                                                                                >  1:3332933/1‑140 (MQ=255)

          cGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccccgta                                                                                                                             <  2:3556922/140‑4 (MQ=255)

          cGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccccggt                                                                                                                             >  2:3240190/1‑137 (MQ=255)

           ggCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACGTgccgccccggtg                                                                                                                            >  2:1759004/1‑136 (MQ=255)

           ggCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccccgtac                                                                                                                            <  1:2032084/140‑5 (MQ=255)

           ggCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccccgtac                                                                                                                            <  2:5398742/140‑5 (MQ=255)

            gCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccccgtacg                                                                                                                           <  1:8792837/140‑6 (MQ=255)

             cATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGAGCTGGCCCCACATccccccccggacgg                                                                                                                          >  2:6750232/1‑134 (MQ=255)

                  cGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATc                                                                                                                   <  1:4334190/140‑1 (MQ=255)

                       cgcATCAGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGAGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAGTCGAggg                                                                                                              >  2:2213665/1‑138 (MQ=255)

                        gcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAgctg                                                                                                             <  1:1560896/140‑1 (MQ=255)

                            cTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGa                                                                                                         <  1:1575497/140‑1 (MQ=255)

                                tAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGc                                                                                                     <  1:2359414/140‑1 (MQ=255)

                                    tGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccccg                                                                                                                               >  2:10528666/1‑111 (MQ=255)

                                    tGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccccg                                                                                                                               <  1:10528667/112‑2 (MQ=255)

                                    tGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGGACGGTAATCGAGCTGCTGACCGCTGat                                                                                                 >  2:6462231/1‑140 (MQ=255)

                                      cGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CTCCCCCCCCGTGCGGTAGTGGGGCTGCGGGCCGCTGattt                                                                                               >  1:9674043/1‑138 (MQ=255)

                                        aCTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCGAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTATTCGAGCTGCGGACCGCTGTTGTcc                                                                                             >  2:8448749/1‑140 (MQ=255)

                                        aCTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAAGCGAGCTGCTGGCCGCTGAGATcc                                                                                             >  2:6634381/1‑140 (MQ=255)

                                        aCTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑GCCCCCCCCGTAGGGGAAGCGAGCTGCTGGCCGCTGAGATcc                                                                                             >  2:4736234/1‑140 (MQ=255)

                                          tGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTATGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑GCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGc                                                                                           <  1:293750/140‑1 (MQ=255)

                                            ttCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCa                                                                                         <  2:8240036/140‑1 (MQ=255)

                                            ttCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCa                                                                                         >  2:5591761/1‑140 (MQ=255)

                                             tCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAg                                                                                        <  2:5005039/140‑1 (MQ=255)

                                             tCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAg                                                                                        <  1:10393150/140‑1 (MQ=255)

                                                 aCGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAg                                                                                        <  1:4104537/136‑1 (MQ=255)

                                                  cGGTTGTTGCGGCAACAATTCTATGAGCGATTTCTCTGGATTCGACGCGATCTGCGCCAGCCTTGCTGCGCCGAGTGCGGGCCCCACAT‑‑CCCCCACCGTACGGTAATCGAGCTGCTGACCGCTGATATGCGCCAGCATCt                                                                                   <  2:1953045/140‑1 (MQ=255)

                                                  cGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGCTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                   <  2:6364988/140‑1 (MQ=255)

                                                  cGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                   <  1:5591762/140‑1 (MQ=255)

                                                  cGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                   <  1:3343713/140‑1 (MQ=255)

                                                  cGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGGGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                   <  2:9098774/140‑1 (MQ=255)

                                                  cGGTAGTTGCGGCAACAAGTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                   <  2:6364928/140‑1 (MQ=255)

                                                    gTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCAGACCGCGGATATCCGCCAGCAGCTGa                                                                                 >  2:847839/1‑140 (MQ=255)

                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTGATCGAGCTGCTGGCCGCTGGTGTCCGGCAGCATCTGACg                                                                               >  2:681382/1‑140 (MQ=255)

                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                               <  1:3956065/140‑1 (MQ=255)

                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGGACGGGAATCGAGCTGCTGACCGCTGATATCCGCCAGCCTCTGACg                                                                               >  2:3628152/1‑140 (MQ=255)

                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑GCCCCCCCCGTTCGGTGAGCGAGCGGCTGACCGCTGATATCGGCCAGCATCGGACg                                                                               >  2:10648515/1‑140 (MQ=255)

                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CTCCCCCCCCGGACGGTACTCGAGCTGGTGGCCGCGGCGATCCGCCAGCAGCTGACg                                                                               >  2:2778525/1‑140 (MQ=255)

                                                        ttGCGGCAACAATTCAATGAGCGAGTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGcc                                                                             <  1:5348147/140‑1 (MQ=255)

                                                        ttGCGGCAACAATACAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CTCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCAGCTGTCGcc                                                                             >  1:9836027/1‑140 (MQ=255)

                                                           cGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGt                                                                          >  2:9342649/1‑140 (MQ=255)

                                                             gCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGGACGGGAATCGAGGTGCTGGCCGCGGATGTCCGCCCGCTTCTGACGCCAGGAc                                                                        >  1:5632547/1‑140 (MQ=255)

                                                              caacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTACTCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACt                                                                       <  2:10723839/140‑1 (MQ=255)

                                                              caacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACt                                                                       <  1:6340544/140‑1 (MQ=255)

                                                              caacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACt                                                                       <  1:2068814/140‑1 (MQ=255)

                                                              caacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑GCCCCCCCCGTACGGGAATCGAGCTGCTGGCCGCGGATATGCGCCAGCGGCTGGCGCGAggtct                                                                       >  2:4162849/1‑136 (MQ=255)

                                                              caacaaTTCAACGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACt                                                                       <  1:6284766/140‑1 (MQ=255)

                                                                 caaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑GCCCCCCCCGTACGGTAAGCGCGCTGGTGGCCGCTGATAGACGCCAGCATCTGTCGCCAGGACTCAc                                                                    >  2:2356736/1‑140 (MQ=255)

                                                                         tGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CGCCCCCCGTAGGGGAGTCGCGCTGCTGAGGGCTGTTTTCCGCCAGGCGGTGTCGCCAGTACGCACTTCGcgcc                                                            >  1:5534252/1‑140 (MQ=255)

                                                                          gAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccc                                                           <  2:3645478/140‑1 (MQ=255)

                                                                          gAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccc                                                           <  1:5707737/140‑1 (MQ=255)

                                                                            gCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCGCCCCCGTACGGTACTCGGGCTGCTGACCGGGGAGTTGCGCCCGCTTGTGTGGGCAGTACTCGCTACGcgccccg                                                         >  2:7946319/1‑140 (MQ=255)

                                                                            gCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTACTCGAGCTGCTGACCGCTGATATCCGCCCGCATCGGACGCCCGTCCTCACTACGcgccccg                                                         >  2:7946542/1‑140 (MQ=255)

                                                                              gATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAg                                                                                                                <  2:5985962/83‑1 (MQ=255)

                                                                              gATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAg                                                                                                                >  1:5985963/1‑83 (MQ=255)

                                                                              gATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CTCCCCCCCCGTACGGTAATCGAGCTGCTGTCCGCTGATATCCGCCTGCATCTGACGCCAGTTCTCTCTACGcgccccgcc                                                       >  2:9469351/1‑140 (MQ=255)

                                                                              gATGTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CTGCGCCCCCGTCCGGTCAGGGCGCGGCGGACGGGTGATATCCGCGCGCATCGGTCGCCAGTACTCTCGACGcgccccgcc                                                       >  2:3355159/1‑140 (MQ=255)

                                                                               aTTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCTCAT‑‑CCCCCCCCGGACGGTTATCGAGCTGCGGACCGCTGATATCAGCCAGCAGCTGACGCCAGTCCTCTCTACGcgccccgccc                                                      >  2:3531139/1‑140 (MQ=255)

                                                                                    tctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                                 <  1:10747856/140‑1 (MQ=255)

                                                                                    tctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                                 <  1:3974874/140‑1 (MQ=255)

                                                                                    tctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                                 <  1:2501527/140‑1 (MQ=255)

                                                                                    tctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                                 <  1:2114193/140‑1 (MQ=255)

                                                                                    tctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                                 <  1:11063922/140‑1 (MQ=255)

                                                                                    tctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                                 <  1:10747846/140‑1 (MQ=255)

                                                                                    tctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                                 <  1:10385710/140‑1 (MQ=255)

                                                                                    tctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGACCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGACCCGCCCCCAAt                                                 <  2:9168847/140‑1 (MQ=255)

                                                                                     ctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑GCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                                 <  2:3139851/140‑1 (MQ=255)

                                                                                             gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCG‑CCCCAATCAACGTAaca                                       >  1:9415964/1‑140 (MQ=255)

                                                                                              ccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccc                                                                                                                                     <  2:2124617/48‑1 (MQ=255)

                                                                                              ccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑GCCCCCCCGTACGGTAATCGAGCTGCTGACCGCGGATATCCGCCAGCATCTGACGCCCGGACTCACTACGCGCCCCGCCCCCATTCACCGGAacc                                       >  2:6009535/1‑139 (MQ=255)

                                                                                                   ttCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGTCGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg                                  <  2:412538/139‑1 (MQ=255)

                                                                                                   aTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg                                  <  1:8837345/140‑1 (MQ=255)

                                                                                                     cTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCg                                <  1:4571349/140‑1 (MQ=255)

                                                                                                      tGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTGATCGAGCTGCTGACCGCTGATATCCGCCAGCTTCGGGCGCCCGTCCGCACTACGCGCCCCGCCCCCAAGCGACGTAACACTTTGCgg                               >  1:10021907/1‑140 (MQ=255)

                                                                                                              ccTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTCCTCGAGCTGCTGACCGCTGATATCCGCCCGCATCTGACGCCAGTACTCCATACGCGCCCCGCCGCCAAGCTACGGGCCACGTTGCGGGTtactac                       >  2:7871949/1‑140 (MQ=255)

                                                                                                              ccTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGTGCTGCGGACCGCTGATATCCGCCCGCATCTGACGCCCGTAGTCACGACGCGCCCCGCCCCCAATCAACGGAACACTTTGCGGTTTAATag                       >  2:6653556/1‑139 (MQ=255)

                                                                                                              ccTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCCCTCAACGGAACAATGTGCGGTTTAATAc                       >  2:2160947/1‑140 (MQ=255)

                                                                                                              ccTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGGAATCGAGCTGCTGACCGCTGATATCCGCCAGCCTCTGGCGCCGGTACTCACGACGCGCCCCGCCCCCCATCAACGGGACCCGTTGCGGGTTAAGAc                       >  2:1862737/1‑140 (MQ=255)

                                                                                                                  gctgcGCCCAGTGCTGGCCCCAC‑‑CTCCCCCCCCGTACGGTAATCGCGCTGCTGACCGCGGATTGCCGCCAGCAGCTGACGCCAGTACGCACTACGCGCCCCGCCCCCAATCAACGTCACACTGTGCGGGTGAATACCGCa                   >  2:7287880/1‑140 (MQ=255)

                                                                                                                   ctgcGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAg                  <  2:4832109/140‑1 (MQ=255)

                                                                                                                        cccAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGATGACCGCTGTTATCCGCCAGCATATGACGCCAGTAATAAATACGCGCCCCGCCCCCAATCAACGTAACTCTTTGCGGTGTAATACCGCAGGCATg             >  2:11404738/1‑140 (MQ=255)

                                                                                                                        cccAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGGCATCGAGCTGCTGCCCGGTGGTATCCGCCAGCATCTGGCGCCAGTACTCTCTACGCGCCCCGCCCCCCGTCAGCGTGACACTTTGCGGGGTAATACCGCGGGCATg             >  1:4309214/1‑140 (MQ=255)

                                                                                                                        cccAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGGAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCGGACGCCAGTGCTCACTGCGCGCCCCGCCCCCAATCAACGGAACACGTGGCGGTGTAATACCGCAGGCATg             >  1:2890846/1‑140 (MQ=255)

                                                                                                                          cAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCa           <  1:795663/140‑1 (MQ=255)

                                                                                                                           aGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCCGCATCTGACGCCCGTACTCACTACGCGCCCCGCCCCCAATCCACGTCGCACTTTGCGGTGTAATACCGCAGGCAAGCAc          >  2:4891760/1‑140 (MQ=255)

                                                                                                                           aGTGCTGGCCCCAC‑‑GGCCCCCCCCGGCCGGGACTCGAGCGGCTGACCGCGGAGATCCGCCCGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGGAACCCTGTGCGGTTGTATACCGCCGGCATGCAc          >  1:3256492/1‑140 (MQ=255)

                                                                                                                             tGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCGCGTACGCACTACGCGCCCCTCCCCCAATCAACGGAACACGTTGAGGTTTAATACGGCAGGCATGCACGa        >  2:4026088/1‑140 (MQ=255)

                                                                                                                             tGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGa        <  1:9442579/140‑1 (MQ=255)

                                                                                                                             tGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGAGATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCTATCAACGTCACACTTTGCGGGTTGATACCGCAGGGATGCCCgg        >  1:2835916/1‑139 (MQ=255)

                                                                                                                             tGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCGGATATCCGCCAGCATCTGACGCCAGGACGCACTCCGCGCCCCGCCCCCAAGCAACGTAACACTGTGCGGTTTAATCGCGCGGGCAGGCACgc        >  2:2587868/1‑139 (MQ=255)

                                                                                                                                  gCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACTCTTTGCGGTTTAATACCGCAGGCGTGCACGACATcg   >  1:9184848/1‑139 (MQ=255)

                                                                                                                                    cccACAT‑CCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCa  <  1:4856802/140‑1 (MQ=255)

||

GGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCA  >  NZ_CP025268/3728661‑3728933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`