BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	4,331,820	(G)_7→6	pseudogene (473/817 nt)	rdcB →	YjcZ‑like family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	4,331,814	0	G	.	98.4%	3408.0 / 33.2	728	pseudogene (467/817 nt)	rdcB	YjcZ‑like family protein
Reads supporting (aligned to +/- strand): ref base G (5/7); new base . (292/422); total (298/430)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 765 total aligned reads displayed.

GAAAAGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAA‑‑CGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGTCACTATTACC  >  NZ_CP025268/4331675‑4331949

  aaaGGCGTTAACGCAACGAGGCCTACTTGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTAGAA‑‑CGCCTGGTCGGTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTggg                                                                                                                                       <  2:10474099/140‑1 (MQ=255)

    aGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAA‑‑CGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTggggg                                                                                                                                     <  1:9816536/140‑1 (MQ=255)

      gCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAA‑‑CGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTggggggc                                                                                                                                   <  1:11047696/140‑2 (MQ=255)

        gTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAA‑‑CGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTggggggcat                                                                                                                                 >  1:10112770/1‑137 (MQ=255)

        gTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAA‑‑CGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTggggggcat                                                                                                                                 >  1:447520/1‑137 (MQ=255)

            aCGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAA‑‑CGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTgg                                                                                                                            <  2:8894848/140‑1 (MQ=255)

             cGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAA‑‑CGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGc                                                                                                                           <  1:6415593/140‑1 (MQ=255)

                   gAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAA‑‑CGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGAtgcc                                                                                                                     <  1:1825276/140‑1 (MQ=255)

                    aGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAA‑‑CGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGAtgcct                                                                                                                    <  1:9192608/140‑1 (MQ=255)

                    aGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAA‑‑CGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGAtgcct                                                                                                                    <  1:8469894/140‑1 (MQ=255)

                    aGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAA‑‑CGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGAtgcct                                                                                                                    <  1:7443206/140‑1 (MQ=255)

                    aGGCCGACTGGTGCAGGCAGGTGATCAGAGCGAGCAAAATTTTACCCGCTGGCGTGCTGGGGCGTATAA‑‑CGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGAtgcct                                                                                                                    <  1:6507934/140‑1 (MQ=255)

                     ggCCGACTGGTGCAGGCAGGTGATCAGAGCCAGCAAATTATTACCCGCTGGCGTGCTGGGGCGTATAA‑‑CGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGAtgcctg                                                                                                                   <  1:6189179/140‑1 (MQ=255)

                          aCTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTATTACCCGCTGGCGTGCTGGGGCGTATAA‑‑CGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTc                                                                                                              <  1:8710404/140‑1 (MQ=255)

                              gTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAA‑‑CGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTggg                                                                                                          <  1:4938447/140‑1 (MQ=255)

                              gTGCAGGCAGGTGATCAGAGCGAGCAAATATTTACCCGCTGGCGTGCTGGGGCGTATAA‑‑CGCCTGGTCGTGGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGAGGCCTGCCGTCTggg                                                                                                          <  2:3755412/140‑1 (MQ=255)

                               cgcaggcagGCGCTCCGAGCGAGCACCTGATGACCCGCTGGCGTGCTGGGGCGTATCA‑‑CGCCTGGACGTTGCCTGGGCGCAGCTGTCTCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTAGGCGATGCCTGCCGTCTGGGa                                                                                                         <  2:9782899/139‑1 (MQ=255)

                                gcaggcagGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAA‑‑CGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGac                                                                                                        >  1:663804/1‑139 (MQ=255)

                                 caggcaggTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAA‑‑CGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAg                                                                                                       >  2:4364205/1‑140 (MQ=255)

                                 caggcaggTGATCAGAGCGAGCAAATTTTTACACGCTGTCGTGCTGGGGCGTATAA‑‑AGCCTGGTCGTTGCATGGGCGCTGTTTTATCGTTCTGGAGGAGTTGAGAT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGTAg                                                                                                       >  2:6569071/1‑140 (MQ=255)

                                  aggcaggTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAA‑‑CGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGc                                                                                                      >  2:5217618/1‑140 (MQ=255)

                                      aggTGATCAGAGCGAGCAAATTATTACCCGCTGGCGTGCTGGGGCGTATAA‑‑CGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGc                                                                                                  <  2:10867731/140‑1 (MQ=255)

                                       ggTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAA‑‑CGCCTGGTCGGTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCa                                                                                                 <  2:1793104/140‑1 (MQ=255)

                                        gTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGc                                                                                                  >  1:8119878/1‑140 (MQ=255)

                                        gTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAA‑‑CGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCaa                                                                                                >  1:6962719/1‑140 (MQ=255)

                                        gTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAA‑‑CGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCaa                                                                                                >  2:1367901/1‑140 (MQ=255)

                                         tGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAA‑‑CGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAg                                                                                               >  1:5892441/1‑140 (MQ=255)

                                         tGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAA‑‑CGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAg                                                                                               <  1:5640865/140‑1 (MQ=255)

                                         tGATCAGAGCGAGCAAATAATTACCCGCTGGCGTGCTGGGGCGTATAA‑‑CGCCTGGTCGTTGCCTGGGCGCTGTTTTAACGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAg                                                                                               <  1:10703061/140‑1 (MQ=255)

                                           aTCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAA‑‑CGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCg                                                                                                                >  1:7669586/1‑121 (MQ=255)

                                             cAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAA‑‑CGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGt                                                                                           <  1:10275251/140‑1 (MQ=255)

                                              agagaaagcAAATTTTTACCCGCTGGCGTGCTGGGGAGTATAA‑‑CGCATGGTCGTTGCCTGGGCGCTGTTTTAACGTTCTGTAGGAGTAGCTCT‑GGGGGGCATTTGGCGATGCATGCAGTCTGGGAAGCCCGCAATCGGTg                                                                                          >  2:10124004/7‑140 (MQ=255)

                                              agagCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAA‑‑CGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTg                                                                                          <  1:5179806/140‑1 (MQ=255)

                                                  cgagcAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAA‑‑CGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCgt                                                                                      >  1:7917390/1‑140 (MQ=255)

                                                   gagcAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAA‑‑CGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCgtt                                                                                     <  1:3943011/140‑1 (MQ=255)

                                                     gcAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAA‑‑CGCCTGGTCGTTGCCTGGGCGCTGTTGTATCGTTCTGGAGGAGTTGCGC‑GGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCgttgt                                                                                   >  2:538367/1‑140 (MQ=255)

                                                          tttttACCCGCTGGCGTGCTGGGGCGTATAA‑‑CGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTg                                                                              <  1:4695530/140‑1 (MQ=255)

                                                                   gCTGGCGTGCTGGGGCGTATAA‑‑CGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTg                                                                     >  2:10686216/1‑140 (MQ=255)

                                                                        cGTGCTGGGGCGTATAA‑‑CGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGAGTTGTTGCTGGGTGATTTGCTCga                                                                <  1:2499822/140‑1 (MQ=255)

                                                                             tGGGGCGTATAA‑‑CGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAg                                                           <  2:2199280/140‑1 (MQ=255)

                                                                             tGGGGCGTATAA‑‑CGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAg                                                           <  2:9466758/140‑1 (MQ=255)

                                                                             tGGGGAGTATAT‑‑CGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAg                                                           <  2:8202217/140‑1 (MQ=255)

                                                                             cggggCGTATAC‑‑CGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGCGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCTAGCGTTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAg                                                           <  2:8698536/139‑1 (MQ=255)

                                                                              ggggCGTATAA‑‑CGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGc                                                          >  1:9525895/1‑140 (MQ=255)

                                                                               gggCGTATAA‑‑CGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCg                                                         >  1:11286005/1‑140 (MQ=255)

                                                                               gggCGTATAA‑‑CGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCg                                                         >  1:3585115/1‑140 (MQ=255)

                                                                                 gCGTATAA‑‑CGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGac                                                       >  1:8267622/1‑140 (MQ=255)

                                                                                  cGTATAA‑‑CGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGaca                                                      <  2:1974738/140‑1 (MQ=255)

                                                                                    tataA‑‑CGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGc                                                                                >  2:3845742/1‑112 (MQ=255)

                                                                                    tataA‑‑CGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGAcaca                                                    >  1:3407340/1‑140 (MQ=255)

                                                                                    tataA‑‑CGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGAcaca                                                    <  1:2872033/140‑1 (MQ=255)

                                                                                    tataA‑‑CGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGAcaca                                                    <  1:1202420/140‑1 (MQ=255)

                                                                                    tataA‑‑CGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGAcaca                                                    <  2:11107809/140‑1 (MQ=255)

                                                                                    tataA‑‑CGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGAcaca                                                    <  1:52765/140‑1 (MQ=255)

                                                                                      taA‑‑CGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGAt                                                                        <  1:10881084/118‑1 (MQ=255)

                                                                                        a‑‑CGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACAt                                                <  2:9209935/140‑1 (MQ=255)

                                                                                             ccTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGGTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTg                                             >  1:4130151/1‑140 (MQ=255)

                                                                                              cTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTgg                                            >  1:482823/1‑140 (MQ=255)

                                                                                              cTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTgg                                            <  2:5071757/140‑1 (MQ=255)

                                                                                              cTGGTCGTTGCCTGGGCGCTGTTTGAGCGTTCTGGAGGAGTAGCGC‑GGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACACCAACTgg                                            <  2:8527722/140‑1 (MQ=255)

                                                                                              cTGGTCGGTGCCTGGGCGCTGGATGATCGTTCTGGAGGAGGTGCGCT‑GGGGGGCCTTTGGCGAGGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTgg                                            <  2:7241641/140‑1 (MQ=255)

                                                                                                ggTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCa                                          >  2:7776998/1‑140 (MQ=255)

                                                                                                ggTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCa                                          <  2:5717868/140‑1 (MQ=255)

                                                                                                ggTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCa                                          >  1:10477459/1‑140 (MQ=255)

                                                                                                ggTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCa                                          <  1:8842236/140‑1 (MQ=255)

                                                                                                ggTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCa                                          <  1:5046290/140‑1 (MQ=255)

                                                                                                ggTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCa                                          <  1:3098908/140‑1 (MQ=255)

                                                                                                ggTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCa                                          >  1:2554188/1‑140 (MQ=255)

                                                                                                ggTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCa                                          <  1:6802952/140‑1 (MQ=255)

                                                                                                ggTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCGGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCa                                          >  1:7091214/1‑140 (MQ=255)

                                                                                                 gTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCACGCGGTGGCGTTGTTGCTGTGTGATTTGCTCGAGAAAGCGACACAACATCTGGCag                                         >  2:6421624/1‑140 (MQ=255)

                                                                                                  tCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCaga                                        <  1:2230756/140‑1 (MQ=255)

                                                                                                      tGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGt                                    >  1:3796311/1‑140 (MQ=255)

                                                                                                        ccggggCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCGGGCAGAGAGTAt                                  <  2:7585892/137‑1 (MQ=255)

                                                                                                        ccTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTAt                                  <  2:4892092/140‑1 (MQ=255)

                                                                                                        ccTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTAt                                  <  2:5878451/140‑1 (MQ=255)

                                                                                                        ccTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTAt                                  <  1:1936861/140‑1 (MQ=255)

                                                                                                            ggCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAAt                              <  1:9630707/140‑1 (MQ=255)

                                                                                                                   ttttATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCAc                       >  2:337099/1‑140 (MQ=255)

                                                                                                                    tttATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCAcc                      <  2:2895027/140‑1 (MQ=255)

                                                                                                                       aTCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGAc                   <  1:4271800/140‑1 (MQ=255)

                                                                                                                        tCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGAcc                  <  2:7443302/140‑1 (MQ=255)

                                                                                                                        tCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGAcc                  >  2:3155444/1‑140 (MQ=255)

                                                                                                                         cGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCa                 <  1:2100092/140‑1 (MQ=255)

                                                                                                                             cTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCTAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCAcgcg             <  2:4664953/140‑1 (MQ=255)

                                                                                                                             cTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGaca                                                      >  1:8980869/1‑99 (MQ=255)

                                                                                                                             cTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGAcaca                                                    <  2:1604270/101‑1 (MQ=255)

                                                                                                                             cTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGAcaca                                                    >  1:1604270/1‑101 (MQ=255)

                                                                                                                             cTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCAcgcg             >  1:1419260/1‑140 (MQ=255)

                                                                                                                             cTGGAGGAGTTGCGCT‑GGGGGGCAATTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCGGGGTGATTTGCTCGAGACAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCAcgcg             >  2:6889314/1‑140 (MQ=255)

                                                                                                                              ttgaggagTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGTTTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGt            <  2:11282058/138‑1 (MQ=255)

                                                                                                                              tGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGACAGCGACACAACATCGGGCAGAGAGTATCAATGCGGCACCGACCACGCGt            <  1:5424553/140‑1 (MQ=255)

                                                                                                                              tGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGt            <  2:6100034/140‑1 (MQ=255)

                                                                                                                              tGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGt            <  2:3942998/140‑1 (MQ=255)

                                                                                                                              tGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGt            <  2:356129/140‑1 (MQ=255)

                                                                                                                              tGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGt            <  2:2636477/140‑1 (MQ=255)

                                                                                                                              tGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGt            <  1:462847/140‑1 (MQ=255)

                                                                                                                                gaggagTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGTCa          <  2:7935449/140‑1 (MQ=255)

                                                                                                                                gaggagTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGTCa          <  2:2333019/140‑1 (MQ=255)

                                                                                                                                     gTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGTCACTAtt     <  1:7525899/140‑1 (MQ=255)

GAAAAGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAA‑‑CGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGTCACTATTACC  >  NZ_CP025268/4331675‑4331949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`