BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	581,197	+G	intergenic (+50/+4)	CXP41_RS24355 → / ← CXP41_RS03085	tail fiber assembly protein/class I SAM‑dependent methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	581,196	1	.	G	98.4%	3075.6 / 32.0	894	intergenic (+49/+5)	CXP41_RS24355/CXP41_RS03085	tail fiber assembly protein/class I SAM‑dependent methyltransferase
Reads supporting (aligned to +/- strand): ref base . (9/5); new base G (502/377); total (511/383)
Fisher's exact test for biased strand distribution p-value = 7.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 916 total aligned reads displayed.

AGCCTGGAAAAAATATCGGGTGTTGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑T‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCTTTCT  >  NZ_CP025268/581057‑581331

  ccTGGAAAAAATATCGGGTGTTGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑T‑gg                                                                                                                                       >  2:2181704/1‑139 (MQ=255)

                cGGGTGTTGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTc                                                                                                                          >  2:2512866/1‑140 (MQ=255)

                 gggTGTTGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGCTTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTcc                                                                                                                         >  2:7944274/1‑140 (MQ=255)

                 gggTGTTGCTGAACCGTGTTGATACATCAACTGCACCTGAGATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTcc                                                                                                                         >  1:256593/1‑140 (MQ=255)

                   gtgtTGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTg                                                                                                                       >  1:3928121/1‑140 (MQ=255)

                   gtgtTGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTg                                                                                                                       <  1:9695307/140‑1 (MQ=255)

                          tGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGa                                                                                                                <  1:688074/140‑1 (MQ=255)

                           gAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACCTGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGaa                                                                                                               <  1:2795228/140‑1 (MQ=255)

                           gAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGaa                                                                                                               <  1:10215471/140‑1 (MQ=255)

                           gAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGaa                                                                                                               <  1:1058426/140‑1 (MQ=255)

                           gAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGaa                                                                                                               <  2:8695018/140‑1 (MQ=255)

                           gAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCAAGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGaa                                                                                                               <  2:4486483/140‑1 (MQ=255)

                            aaCCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGaa                                                                                                               >  2:1166722/1‑139 (MQ=255)

                              ccGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAAc                                                                                                            >  1:756653/1‑140 (MQ=255)

                                gtgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                          <  1:8590636/140‑1 (MQ=255)

                                gtgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                          >  1:8346466/1‑140 (MQ=255)

                                 tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACAtt                                                                                                         >  2:4787424/1‑140 (MQ=255)

                                 tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACAtt                                                                                                         >  2:5193750/1‑140 (MQ=255)

                                 tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACAtt                                                                                                         >  2:4187868/1‑140 (MQ=255)

                                 tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACAtt                                                                                                         >  2:8869826/1‑140 (MQ=255)

                                 tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACAtt                                                                                                         >  1:4075966/1‑140 (MQ=255)

                                      aTACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATtactac                                                                                                    >  1:1998505/1‑140 (MQ=255)

                                      aTACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATtactac                                                                                                    >  1:1560999/1‑140 (MQ=255)

                                       tACATCAACTGCCCCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACa                                                                                                   <  1:8046886/140‑1 (MQ=255)

                                       tACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACa                                                                                                   <  2:7086676/140‑1 (MQ=255)

                                       tACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACa                                                                                                   <  1:2181705/140‑1 (MQ=255)

                                       tACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACa                                                                                                   <  1:8732093/140‑1 (MQ=255)

                                        aCATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAg                                                                                                  >  1:3586035/1‑140 (MQ=255)

                                         cATCAACTGCACCTGATATTGAGTCGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGNAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGt                                                                                                 <  1:6042568/140‑1 (MQ=255)

                                           tCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGTAAACATTACTACAGTAc                                                                                               >  1:9965412/1‑140 (MQ=255)

                                           tCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTAc                                                                                               >  1:9223227/1‑140 (MQ=255)

                                           tCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTAc                                                                                               >  1:2406694/1‑140 (MQ=255)

                                           tCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTAc                                                                                               >  2:7633735/1‑140 (MQ=255)

                                           tCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTAc                                                                                               >  2:11208235/1‑140 (MQ=255)

                                           tCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGACGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTag                                                                                               >  2:6490983/1‑139 (MQ=255)

                                            cAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTAcc                                                                                              <  1:11043735/140‑1 (MQ=255)

                                                   aCCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAAcc                                                                                       >  2:9520642/1‑140 (MQ=255)

                                                   aCCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAAcc                                                                                       <  2:9210085/140‑1 (MQ=255)

                                                   aCCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAAcc                                                                                       >  2:10445011/1‑140 (MQ=255)

                                                    ccTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCt                                                                                      >  1:7144863/1‑140 (MQ=255)

                                                    ccTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCt                                                                                      <  2:4005558/140‑1 (MQ=255)

                                                    ccTGATATTGAGTCGCCTACGAACCCAGACAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAATCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCt                                                                                      <  2:6438134/140‑1 (MQ=255)

                                                        atatTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGt                                                                                  >  1:11364451/1‑140 (MQ=255)

                                                            tGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTa                                                                              >  1:7816828/1‑140 (MQ=255)

                                                             gAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTaa                                                                             <  2:4342257/140‑1 (MQ=255)

                                                              aGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGTTTTTAAc                                                                            >  2:8275413/1‑140 (MQ=255)

                                                              aGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAAc                                                                            >  1:1790544/1‑140 (MQ=255)

                                                               gTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGTACCTTGGTTTTAAcg                                                                           >  2:8518888/1‑139 (MQ=255)

                                                                tGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACAt                                                                          >  2:3098110/1‑140 (MQ=255)

                                                                  gCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATtc                                                                        >  1:10020995/1‑140 (MQ=255)

                                                                  gCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATtc                                                                        >  2:5891732/1‑140 (MQ=255)

                                                                  gCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGAGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATtc                                                                        >  2:6715557/1‑140 (MQ=255)

                                                                   ccTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTTACATtct                                                                       >  1:9421668/1‑140 (MQ=255)

                                                                      aCGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGa                                                                    <  2:11374926/140‑1 (MQ=255)

                                                                       cGACCCCTGTCACGGAGGACTCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGCACATGCTGCGTT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGACCCTTGGTTTTAACATTCTCGaa                                                                   <  2:6168716/140‑1 (MQ=255)

                                                                       cGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGaa                                                                   <  1:5061007/140‑1 (MQ=255)

                                                                        gAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGaaa                                                                  <  1:11251076/140‑1 (MQ=255)

                                                                            ccTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGct                                                              <  1:3643450/140‑1 (MQ=255)

                                                                            ccTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGct                                                              <  1:6992414/140‑1 (MQ=255)

                                                                             cTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctc                                                             >  2:4950073/1‑140 (MQ=255)

                                                                             cTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctc                                                             >  1:8924692/1‑140 (MQ=255)

                                                                             cTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctc                                                             >  1:8238621/1‑140 (MQ=255)

                                                                             cTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctc                                                             >  2:9763309/1‑140 (MQ=255)

                                                                              tGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAAt                                                                 <  2:1900587/135‑1 (MQ=255)

                                                                                tCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTga                                                          <  1:6655743/140‑1 (MQ=255)

                                                                                tCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTga                                                          <  1:5907913/140‑1 (MQ=255)

                                                                                 cAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTgag                                                         >  2:5082719/1‑140 (MQ=255)

                                                                                  aGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTgaga                                                        >  2:7453724/1‑140 (MQ=255)

                                                                                  aGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTgaga                                                        >  1:7685313/1‑140 (MQ=255)

                                                                                      aGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGtat                                                    >  2:11072609/1‑140 (MQ=255)

                                                                                      aGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGtat                                                    <  1:4206545/140‑1 (MQ=255)

                                                                                      aGTAAGCATAGGGCTTCTGCCGCAGCACGTCTTAAGCAAGAACATGCTTCGGTAG‑GATGCTATTATTTTCCTGAAGCGGAAAACATTACTACAGTACCTAGAACCGTGGTTTAATCATTCACGAAATGCTCTGAGAGtat                                                    >  2:2772248/1‑140 (MQ=255)

                                                                                       gTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGtata                                                   <  1:5290679/140‑1 (MQ=255)

                                                                                       gTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGtata                                                   <  1:1377364/140‑1 (MQ=255)

                                                                                        tAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTatat                                                  >  2:9862263/1‑140 (MQ=255)

                                                                                          aTCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGtat                                                    >  2:3810057/1‑136 (MQ=255)

                                                                                          aTCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTA‑TTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATAtgtg                                               >  1:5615147/1‑140 (MQ=255)

                                                                                             aTTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGtt                                             <  2:5680367/140‑1 (MQ=255)

                                                                                              ttGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTa                                            >  2:3347584/1‑140 (MQ=255)

                                                                                               tGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACATTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTaa                                           >  2:910145/1‑140 (MQ=255)

                                                                                                gggATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACAt                                                                          >  1:1170600/1‑108 (MQ=255)

                                                                                                  gATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTaa                                                                             >  2:467999/1‑103 (MQ=255)

                                                                                                   aTTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCct                                       <  1:4483960/140‑1 (MQ=255)

                                                                                                            cagcaCGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGaa                              >  1:4838592/1‑140 (MQ=255)

                                                                                                                 cGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGtt                                             <  2:10875689/120‑1 (MQ=255)

                                                                                                                    cTTAAGCAAGAACCTGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAACTGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTtgg                      >  2:1681869/1‑139 (MQ=255)

                                                                                                                        aGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTGTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGttt                  <  2:8964151/140‑1 (MQ=255)

                                                                                                                         gCAAGAACAAGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCct                                       >  1:6278034/1‑118 (MQ=255)

                                                                                                                            aGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGaaa              >  2:1414263/1‑140 (MQ=255)

                                                                                                                            aGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGaaa              >  1:10734380/1‑140 (MQ=255)

                                                                                                                            aGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTAGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGAGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGaaa              >  2:5411985/1‑140 (MQ=255)

                                                                                                                             gAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGaaaa             >  2:7328095/1‑140 (MQ=255)

                                                                                                                             gAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGaaaa             >  1:7356789/1‑140 (MQ=255)

                                                                                                                              aaCATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAg            >  1:328213/1‑140 (MQ=255)

                                                                                                                              aaCATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAg            >  2:10249621/1‑140 (MQ=255)

                                                                                                                              aaCATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAg            >  1:2973756/1‑140 (MQ=255)

                                                                                                                              aaCATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAg            >  2:3587169/1‑140 (MQ=255)

                                                                                                                              aaCATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAg            >  1:7208673/1‑140 (MQ=255)

                                                                                                                                       cGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATtacta                                                                                                     >  1:10369777/1‑42 (MQ=255)

                                                                                                                                       cGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCTTTc   >  2:10691753/1‑140 (MQ=255)

AGCCTGGAAAAAATATCGGGTGTTGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑T‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCTTTCT  >  NZ_CP025268/581057‑581331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`