BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	939,446	(A)_7→8	intergenic (‑289/‑255)	trxB ← / → lrp	thioredoxin‑disulfide reductase/leucine‑responsive transcriptional regulator Lrp

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	939,439	1	.	A	97.4%	4544.5 / 85.9	1212	intergenic (‑282/‑262)	trxB/lrp	thioredoxin‑disulfide reductase/leucine‑responsive transcriptional regulator Lrp
Reads supporting (aligned to +/- strand): ref base . (10/22); new base A (357/821); total (369/843)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1471 total aligned reads displayed.

CTGCTACTTAAATTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑G‑‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTCGATAGCTGCCACAAGGCAACGGTCTTCTC  >  NZ_CP025268/939300‑939572

ctGCTACTTAAATTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGgaa‑g                                                                                                                                           >  1:10393682/1‑140 (MQ=255)

 tGCTACTTAAATTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAATCATTCGAGgaa‑g‑‑a                                                                                                                                        >  1:7708946/1‑140 (MQ=255)

 tGCTACTTAAATTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGgaa‑g‑‑a                                                                                                                                        >  2:10901897/1‑140 (MQ=255)

 tGCTACTTAAATTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGgaa‑g‑‑a                                                                                                                                        >  1:10996259/1‑140 (MQ=255)

   cTACTTAAATTTCCCGCTTTATAAGCCGATTAAATGATTAATAAACGCCCCTGTTAATGAATTTCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑G‑‑aaa                                                                                                                                      <  2:721583/140‑1 (MQ=255)

   cTACTTAAATTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑G‑‑aaa                                                                                                                                      >  2:4526000/1‑140 (MQ=255)

     aCTTAAATTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑G‑‑aaa‑a‑a                                                                                                                                  <  1:11255356/140‑1 (MQ=255)

      cTTAAATTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAATCATTCGAGGAA‑G‑‑aaa‑a‑aa                                                                                                                                 >  1:8074104/1‑140 (MQ=255)

      cTTAAATTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑G‑‑aaa‑a‑aa                                                                                                                                 <  2:8638808/140‑1 (MQ=255)

      cTTAAATTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑G‑‑aaa‑a‑aa                                                                                                                                 >  1:2377201/1‑140 (MQ=255)

        tAAATTTCCCGCTTTATCCGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑G‑‑aaa‑a‑aaaa                                                                                                                               <  1:8422468/140‑2 (MQ=255)

        tAAATTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑G‑‑aaa‑a‑aaaa                                                                                                                               <  2:1277334/140‑2 (MQ=255)

          aaTTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑G‑‑aaa‑a‑aaaaca                                                                                                                             <  2:9569824/140‑4 (MQ=255)

          aaTTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑G‑‑aaa‑a‑aaaaca                                                                                                                             <  2:8018391/140‑4 (MQ=255)

            tttCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑G‑‑aaa‑a‑aaaacagt                                                                                                                           <  1:10004674/140‑6 (MQ=255)

            tttCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑G‑‑aaa‑a‑aaaacagt                                                                                                                           <  2:1492998/140‑6 (MQ=255)

             ttCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAATACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTa                                                                                                                           >  1:572756/1‑140 (MQ=255)

             ttCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTa                                                                                                                           >  1:1277400/1‑140 (MQ=255)

                ccGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTc                                                                                                                        >  1:7072338/1‑140 (MQ=255)

                    tttATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTtat                                                                                                                    <  1:869000/140‑1 (MQ=255)

                    tttATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTtat                                                                                                                    <  2:4716734/140‑1 (MQ=255)

                    tttATAAGCAGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTGTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTtat                                                                                                                    <  2:10702030/140‑1 (MQ=255)

                    attATAAGCCGATTAAATGATGAATACACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTtat                                                                                                                    <  1:7360290/139‑1 (MQ=255)

                      tataAGCCGATTAAATGATGAATAACCGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTatat                                                                                                                  <  1:10780263/140‑1 (MQ=255)

                      tataAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTTTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTAGTCTTatat                                                                                                                  <  2:821991/140‑1 (MQ=255)

                      tataAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTatat                                                                                                                  <  1:7877210/140‑1 (MQ=255)

                      tataAGCCGATTAAATGATGAATAAACGCCCCTGTTAAGGAATATCTGGCATGTTGGACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTGGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTatat                                                                                                                  <  2:6095959/140‑1 (MQ=255)

                              gATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTtat                                                                                                                    <  2:7041898/130‑1 (MQ=255)

                              gATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATgcg                                                                                                               >  2:4101625/1‑135 (MQ=255)

                              gATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCATaa                                                                                                          >  1:2597301/1‑140 (MQ=255)

                                ttAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTtat                                                                                                                    >  1:9794019/1‑128 (MQ=255)

                                 tAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACca                                                                                                       <  1:8190696/140‑1 (MQ=255)

                                 tAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACca                                                                                                       <  2:11432486/140‑1 (MQ=255)

                                   actgatgaATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACcatg                                                                                                     <  1:1854644/138‑1 (MQ=255)

                                   aaTGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACcatg                                                                                                     <  2:10491056/140‑1 (MQ=255)

                                   aaTGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACcatg                                                                                                     <  1:9175768/140‑1 (MQ=255)

                                   aaTGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACcatg                                                                                                     <  2:4310459/140‑1 (MQ=255)

                                   aaTGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACcatg                                                                                                     <  1:7479249/140‑1 (MQ=255)

                                   aaTGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACcatg                                                                                                     <  1:551469/140‑1 (MQ=255)

                                   aaTGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACcatg                                                                                                     <  2:2785387/140‑1 (MQ=255)

                                   aaTGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACcatg                                                                                                     <  2:659275/140‑1 (MQ=255)

                                   aaTGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACcatg                                                                                                     <  1:3476722/140‑1 (MQ=255)

                                   aaTGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACcatg                                                                                                     <  2:9091841/140‑1 (MQ=255)

                                     tgatgaATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACcatgca                                                                                                   <  1:202708/140‑1 (MQ=255)

                                        tgaATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACCATGCATGt                                                                                                <  2:2980842/140‑1 (MQ=255)

                                        tgaATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACCATGCATGt                                                                                                <  2:7771638/140‑1 (MQ=255)

                                         gaATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACcatg                                                                                                     >  1:6881962/1‑134 (MQ=255)

                                            tAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGAGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACCATGCATGTAAAt                                                                                            <  1:5862572/140‑1 (MQ=255)

                                             aaaCGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑G‑‑aaa‑a‑aaaacagt                                                                                                                           <  2:6917911/107‑6 (MQ=255)

                                              caCGCCACTGTTAATGAATATCTTGCATGTTGTACTAAACATCGATTTTTTGCTTTGACAAGCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTAGTCTTATATGCGCATAACCATGCATGTAAATAc                                                                                          <  2:9821111/139‑1 (MQ=255)

                                              aaCGCCCCTGTTAATGAATATCTGGCTTGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑G‑‑AAATA‑AAACAGTATTCTTATATGCGCATAACCATGCATGTAAATAc                                                                                          <  1:9508573/140‑1 (MQ=255)

                                              aaCGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACCATGCATGTAAATAc                                                                                          <  1:4966343/140‑1 (MQ=255)

                                              aaCGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACCATGCATGTAAATAc                                                                                          <  1:3145729/140‑1 (MQ=255)

                                                cGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCa                                                                                        <  2:338347/140‑1 (MQ=255)

                                                cGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCa                                                                                        <  2:1896600/140‑1 (MQ=255)

                                                      tGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTa                                                                                  >  2:5621123/1‑140 (MQ=255)

                                                      tGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTa                                                                                  <  2:3878782/140‑1 (MQ=255)

                                                      tGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTa                                                                                  <  2:4804311/140‑1 (MQ=255)

                                                      tGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTa                                                                                  >  2:5342403/1‑140 (MQ=255)

                                                      tGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTGATATGCGCATAACCATGCATGTAAATACCATGTTTa                                                                                  >  1:5124147/1‑140 (MQ=255)

                                                       gTTAATGAATATCTGGCTTGTTGTAATAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTGTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTAc                                                                                 <  1:6694772/140‑1 (MQ=255)

                                                           aTGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTg                                                                             <  1:1691412/140‑1 (MQ=255)

                                                           aTGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTg                                                                             <  2:7557639/140‑1 (MQ=255)

                                                           aTGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTg                                                                             <  2:932529/140‑1 (MQ=255)

                                                           aTGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTg                                                                             >  2:2575756/1‑140 (MQ=255)

                                                           aTGAATATCTGGCATGTTGTACTAAAAATCGATGTTGTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTg                                                                             <  1:5327246/140‑1 (MQ=255)

                                                                tatCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGt                                                                        >  1:2985206/1‑140 (MQ=255)

                                                                tatCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGt                                                                        >  2:6388860/1‑140 (MQ=255)

                                                                 atCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTg                                                                       <  2:2338834/140‑1 (MQ=255)

                                                                  tCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACCATGCATGTAAAt                                                                                            <  1:4729658/118‑1 (MQ=255)

                                                                  tCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGa                                                                      >  2:3674956/1‑140 (MQ=255)

                                                                  tCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGa                                                                      <  1:3254193/140‑1 (MQ=255)

                                                                  tCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGa                                                                      >  1:1016465/1‑140 (MQ=255)

                                                                  tCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑G‑‑aaa‑a‑aaaaca                                                                                                                             >  2:3216348/1‑81 (MQ=255)

                                                                    tGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACNGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGNTAGTGaaa                                                                    <  1:6065630/140‑1 (MQ=255)

                                                                    tGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGaaa                                                                    <  1:2180522/140‑1 (MQ=255)

                                                                    tGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGaaa                                                                    <  1:6488455/140‑1 (MQ=255)

                                                                      gCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTg                                                                             <  1:4607259/129‑1 (MQ=255)

                                                                      gCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATc                                                                  >  1:11423947/1‑140 (MQ=255)

                                                                      gCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATc                                                                  <  2:576236/140‑1 (MQ=255)

                                                                      gCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑G‑‑AAA‑ACAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAACTc                                                                  <  2:1653139/140‑1 (MQ=255)

                                                                       cATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCt                                                                 <  1:4113970/140‑1 (MQ=255)

                                                                        aTGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTa                                                                >  1:4382851/1‑140 (MQ=255)

                                                                          gttgtACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTa                                                                                  >  2:11072961/1‑120 (MQ=255)

                                                                          gttgtACTAAAAATCGATGTTTTGCGTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACg                                                              >  1:3902019/1‑140 (MQ=255)

                                                                           ttgtACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGACACCATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACCATGCAGGTAAATCCCATGTTTACCGGGCTAGTGAAATCTACGt                                                             <  2:5040109/140‑1 (MQ=255)

                                                                           ttgtACTAAAAATCGATGATTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGt                                                             <  1:3645055/140‑1 (MQ=255)

                                                                             gtACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAACTCTACGTAt                                                           <  1:1563345/140‑1 (MQ=255)

                                                                               aCTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATgg                                                         <  2:7378330/140‑1 (MQ=255)

                                                                                cTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GAAAAA‑A‑AAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATgg                                                         >  2:10039107/1‑140 (MQ=255)

                                                                                cTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACcatg                                                                                                     <  1:2766458/95‑1 (MQ=255)

                                                                                        cGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAg                                                >  2:5936077/1‑140 (MQ=255)

                                                                                         gatttattGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAATA‑‑AAA‑A‑AAACAGTATTCTTATATGCGCATTACCATGCATGGAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGa                                               <  1:6346899/134‑1 (MQ=255)

                                                                                                             cccTGGAGTTTTTCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAAAACTATTCTTATAAGCGCATAACCATGAATGTAAATAACATGTTTACCGTGCTAGTGAAATCTACGTAATGAGTGGACAGACGCACTTCTTGATGTCGATa                           >  2:2146719/1‑140 (MQ=255)

                                                                                                                     ttttGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCAt                                                                                                            >  2:8952227/1‑51 (MQ=255)

                                                                                                                     ttttGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCAt                                                                                                            <  1:8952228/51‑1 (MQ=255)

                                                                                                                     ttttGCGAAAACATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTCGATAGCTGCcac                   >  2:8477329/1‑140 (MQ=255)

                                                                                                                             aaaCATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTCGATAGCTGCCACAAGGCAAc           <  2:10229357/140‑1 (MQ=255)

                                                                                                                              aaCATTCGAGGAA‑GA‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTCGATAGCTGCCACAAGGCAACg          <  1:10509501/140‑1 (MQ=255)

                                                                                                                                cATTCGAGGAA‑GA‑AAA‑A‑AAACAGTACTCGTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTCGATAGCTGCCACAAGGCAACGGt        <  1:7247705/140‑1 (MQ=255)

CTGCTACTTAAATTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAA‑G‑‑AAA‑A‑AAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTCGATAGCTGCCACAAGGCAACGGTCTTCTC  >  NZ_CP025268/939300‑939572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`