BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,374,862	(T)_8→9	intergenic (+40/‑172)	pspE → / → ycjM	thiosulfate sulfurtransferase PspE/glucosylglycerate phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,374,854	1	.	T	92.5%	1710.7 / 136.5	595	intergenic (+32/‑180)	pspE/ycjM	thiosulfate sulfurtransferase PspE/glucosylglycerate phosphorylase
Reads supporting (aligned to +/- strand): ref base . (27/16); new base T (297/234); total (325/270)
Fisher's exact test for biased strand distribution p-value = 4.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.77e-01

Only 100 of 629 total aligned reads displayed.

GACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGA  >  NZ_CP025268/1374716‑1374991

gACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑t                                                                                                                                              >  1:9435493/1‑140 (MQ=255)

      aGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTCGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑tttttt‑t                                                                                                                                       <  1:5879707/140‑1 (MQ=255)

       gTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGACGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑tttttt‑t‑t                                                                                                                                     >  1:628952/1‑140 (MQ=255)

            ggCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGAGGCGCTTATGCGCCG‑‑tttttt‑t‑ttctgt                                                                                                                                >  1:8051192/1‑135 (MQ=255)

             gcaagcaaAAGAGATCCTTAGCGAGTTGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑Tc                                                                                                                              <  2:10520432/140‑1 (MQ=255)

             gcaagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑Tc                                                                                                                              >  1:11199829/1‑140 (MQ=255)

              caagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑Tca                                                                                                                             <  1:10950494/140‑1 (MQ=255)

               aagcaaAAGATATCCTTAGCGAGATGGGATATACCCACGTTGAGTACGCCGGTGGCCTGAAAGACATCTATATTACGAAGGTCATAGGTTAAAAGAATTCACCTTGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑Tcac                                                                                                                            <  2:7554899/140‑1 (MQ=255)

               aagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑Tcac                                                                                                                            <  2:5664012/140‑1 (MQ=255)

               aagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑Tcac                                                                                                                            <  1:1811488/140‑1 (MQ=255)

                 gcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCacac                                                                                                                          >  1:1157346/1‑140 (MQ=255)

                 gcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCacac                                                                                                                          >  2:8965616/1‑140 (MQ=255)

                  caaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGCAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACAcc                                                                                                                         <  1:2889746/140‑1 (MQ=255)

                  caaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACAcc                                                                                                                         <  2:1708061/140‑1 (MQ=255)

                  caaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACAcc                                                                                                                         <  2:10340128/140‑1 (MQ=255)

                  caaAAGAGAGCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGACAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATACACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACAcc                                                                                                                         <  2:9567164/140‑1 (MQ=255)

                   aaaaGAGCTCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGCGCGGCGCTTATGCGCCGTTTTTTTT‑T‑T‑CTG‑TCACAcc                                                                                                                         <  1:8874019/140‑1 (MQ=255)

                         gaTCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTa                                                                                                                  <  1:8656528/140‑1 (MQ=255)

                          aTCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCTCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTT‑T‑TGCTG‑TCACACCTGATTTAc                                                                                                                 >  2:8766789/1‑140 (MQ=255)

                           tCCTTAGCGAGATGGGATATACCCACGTTGAGACCGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTACa                                                                                                                <  1:10230283/140‑1 (MQ=255)

                           tCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCTCAATGCCGAAGGTCAAAGGTTTAAAGAATTCACCATGAGCGGCTCTTATTCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTTTTTACa                                                                                                                <  2:6887784/140‑1 (MQ=255)

                           tCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAATGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTT‑T‑T‑CTG‑TCACACCTTATTTAc                                                                                                                 >  2:3532227/1‑140 (MQ=255)

                           tCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTACa                                                                                                                <  1:7037586/140‑1 (MQ=255)

                           tCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTACa                                                                                                                <  1:10072155/140‑1 (MQ=255)

                           tCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTACa                                                                                                                <  1:8449265/140‑1 (MQ=255)

                           tCCATAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTACa                                                                                                                <  2:9183597/140‑1 (MQ=255)

                             cTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTTGT‑T‑CTG‑TCACACCTTATTTACATc                                                                                                              <  1:2122254/140‑1 (MQ=255)

                                 gCGAGATGGGATATACCCACGTTGAGAACGCCGGTGTCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTACATCCCCa                                                                                                          <  2:7418780/140‑1 (MQ=255)

                                 gCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTACATCCCCa                                                                                                          <  1:319127/140‑1 (MQ=255)

                                 gCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTACATCCCCa                                                                                                          <  1:7762338/140‑1 (MQ=255)

                                   gagaTGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTACATCCCCATa                                                                                                        <  1:10377448/140‑1 (MQ=255)

                                      aTGGGCTATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTGATGCGCCG‑‑TTTTTT‑TGT‑CTG‑TCAGGCCTTATTTACATCCCCAgagag                                                                                                     >  1:9124788/1‑139 (MQ=255)

                                      aTGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTT‑T‑T‑CTG‑TCACACCTTATTTACATCCCCATAGa                                                                                                      >  1:9592739/1‑140 (MQ=255)

                                      aTGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TAACACCGTATTTACATCCCCATAGat                                                                                                     >  2:7813085/1‑140 (MQ=255)

                                      aTGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑ATG‑TCACACCTTATTTACATCCCCATAGat                                                                                                     >  2:10144264/1‑140 (MQ=255)

                                      aTGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTGATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTAAATCCCCATAGat                                                                                                     >  1:7498445/1‑140 (MQ=255)

                                               aCCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTCTGCGCCGT‑TTTTTT‑T‑T‑ATG‑TCACCCCGTATTTAAATCCCCCGAGATTATTTGCGt                                                                                            >  1:4047643/1‑140 (MQ=255)

                                               aCCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTACATCCCCATAGGTTATTTGCGt                                                                                            >  2:400559/1‑140 (MQ=255)

                                               aCCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTACATCCCCATAGATTATTTGCGt                                                                                            >  2:3000776/1‑140 (MQ=255)

                                               aCCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCGTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCGTATTTACATCCCCATAGATTATTTGCGt                                                                                            >  2:4185707/1‑140 (MQ=255)

                                               aCCCACGTGGTGAGCGCCGGTGGCGTGTCAGACCTCGCATTGCCGAAGGTCAATGGTTAGAAGAATTCACCATGAGCGGGGCGGAGGCGCCGT‑TTTTTT‑T‑T‑GTG‑TCACACCATATTTACATCCCCATAGATGATTTGCgg                                                                                            >  2:2563446/1‑139 (MQ=255)

                                                    cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTACATCCCCATAGATTATTTTCGTCAGCt                                                                                       >  1:6818324/1‑140 (MQ=255)

                                                    cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCt                                                                                       >  2:5782670/1‑140 (MQ=255)

                                                    cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTAAATCCCCATAGATTATTTGCGTCAGCt                                                                                       >  2:1469864/1‑140 (MQ=255)

                                                        gagaACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTAAATCCCCAGAGGTTATTTTCGTCAGCTcaca                                                                                   >  1:5612799/1‑140 (MQ=255)

                                                         agaACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCATATTTAAATACCCAAAGATTATTTTCGTCAGCGCACaa                                                                                  >  1:5286510/1‑140 (MQ=255)

                                                            aCGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATa                                                                               <  2:7129824/140‑1 (MQ=255)

                                                            aCGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATa                                                                               <  1:2287746/140‑1 (MQ=255)

                                                            aCGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAACAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATa                                                                               <  2:6412470/140‑1 (MQ=255)

                                                               ccGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTACATCCCCATAGATTATTTGCGGCAGCTCACAAATACGc                                                                            >  2:8153070/1‑140 (MQ=255)

                                                                     gCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCGTATTTAAATCCCCCTAGATTATTTGCGTCAGCTCACAAATACGCtttttg                                                                      >  1:9845765/1‑139 (MQ=255)

                                                                      ccTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTcc                                                                     <  1:3714120/140‑1 (MQ=255)

                                                                        tGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACCCCGTATTTACAACCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTaccg                                                                   >  1:1360967/1‑139 (MQ=255)

                                                                             gACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTACATCCCCATAGATTATTTGCGGCAGCTCACAAATACGCTTTTTCCCTGGTaa                                                              >  2:4308870/1‑140 (MQ=255)

                                                                                aTCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTACATCCCCCTAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTaaaaa                                                           >  2:9850178/1‑140 (MQ=255)

                                                                                aTCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑ATG‑TCACAACTTATTTACATACCCATATATTATTTGCGTCATCTCACAAATAAGCTTTTTCCCTGGGaaaaa                                                           >  1:549914/1‑140 (MQ=255)

                                                                                  cGCAATGCCGAAGGTCAAAGGTTAAAAGACTTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑ATG‑GCACCCCTTTTTTAAAACCCCCTAGATTATTTTCGGCAGCCCACAAAAAAGCGTTTTACCAGGTaaaaaaa                                                         >  1:10636114/1‑139 (MQ=255)

                                                                                  cGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAAt                                                         <  2:323688/140‑1 (MQ=255)

                                                                                       tGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTAAATACCCCAAGATTATTTGCGGCAGCTCACAAATACGCTTTTTCCCTGGGAAAACATGAttt                                                    >  1:10291471/1‑140 (MQ=255)

                                                                                        gCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTc                                                   <  2:4926056/140‑1 (MQ=255)

                                                                                        gCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGA‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTc                                                   <  1:6606980/140‑1 (MQ=255)

                                                                                         ccGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTTTTTACATCCCCCTAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGAtttac                                                  >  2:2262035/1‑138 (MQ=255)

                                                                                             aGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCg                                              <  2:9317435/140‑1 (MQ=255)

                                                                                             aGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCg                                              <  2:2390115/140‑1 (MQ=255)

                                                                                             aGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCg                                              <  2:550020/140‑1 (MQ=255)

                                                                                              ggTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGt                                             <  2:11424908/140‑1 (MQ=255)

                                                                                              ggTCAAAGGTTAAAAGAATTCACCATGAGCGGCGATTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACTAATACGCTTTTTCCCTGGTATAAAATGATTTCCTGCGt                                             <  2:1898611/140‑1 (MQ=255)

                                                                                                   aaGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCGTATTTACATCCCCAAAGATTATTTTCGGCAGCTCACAAAAACGCTTTTTACCGGGTAAAAAATGATTTACTGCGTGACTa                                        >  1:4580878/1‑140 (MQ=255)

                                                                                                    aGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTaa                                       <  2:7044635/140‑1 (MQ=255)

                                                                                                      gTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑GTG‑TCACACCGTTTTTACAAACCCAAAGATTTTTTGAGTCAGCTCACAAAAAAGCGTTTTACCTGGTAAAAAATTATTTACTGCGGGACTaaaa                                     >  1:5694984/1‑140 (MQ=255)

                                                                                                      gTTAAAAGAAATCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTaaaa                                     <  1:2703884/140‑1 (MQ=255)

                                                                                                        tAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAAcc                                   >  2:8279816/1‑140 (MQ=255)

                                                                                                        tAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTT‑T‑T‑TAGTTCACACCGTATTTAAATCCCCATAGATTATGTGCGTCAGCTCACAAATAAGCTTTTTCCCTGGTTAAAAATGATTTCCTGCGTGACTAAAAcc                                   >  1:4791254/1‑140 (MQ=255)

                                                                                                         aaaaGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTACATCCCCATAGATTATTTTCGTCAGCTCACAAATACGCTTTTTCCCAGGTAAAAAATGATTTCCTGCGTGACTAAAAcca                                  >  2:6149340/1‑139 (MQ=255)

                                                                                                         aaaaGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAAccc                                  <  1:722085/140‑1 (MQ=255)

                                                                                                         aaaaGAATTCACCATGAGCGGCGCGTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCGTATTTACATACCCATAGATTATTTTCGTCAGCACACAAATACGCGTTTTACCTGGGAAAAAAATATTTGCTGCGTGTCTAAAAacc                                  >  1:5399917/1‑140 (MQ=255)

                                                                                                          aaaGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAAcccg                                 >  2:6336386/1‑139 (MQ=255)

                                                                                                          aaaGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCt                                 >  2:457823/1‑140 (MQ=255)

                                                                                                             gAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAACTACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTtgt                              <  2:3719299/140‑1 (MQ=255)

                                                                                                             gAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTtgt                              <  2:5081223/140‑1 (MQ=255)

                                                                                                             gAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTtgt                              <  1:10736661/140‑1 (MQ=255)

                                                                                                               aTTCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGc                            <  1:4176667/140‑1 (MQ=255)

                                                                                                                ttCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCt                           >  2:8523344/1‑140 (MQ=255)

                                                                                                                ttCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCAAACCTTATTTACATCCCCATATATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTtttgat                           >  2:11138373/1‑138 (MQ=255)

                                                                                                                ttCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑ATG‑TCACACCATAATTAAATCCCCAAAGATTATTTGCGTCAGCTCACAAAAAAGCTTTTTACCTGGTAAAAAATGATTTACAGCGTGAATAAAACCCTTTTGCt                           >  1:338529/1‑140 (MQ=255)

                                                                                                                 tCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑GTG‑TCACACCTTTTTTTCATCACCATATTTTATTTGCGTCAGCGCACAAATAAGCATTTTACAAGGTAAAAAATGTTTTAAAGCGTGAAAAAAACACATGTGCTc                          >  1:4426431/1‑140 (MQ=255)

                                                                                                                 tCACCATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑ATG‑TCACACCATATTTACAACCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTACCTGGTAAAAAATTATTTCCTGCGTGACTAAAAACCATGTGCta                          >  1:3835658/1‑139 (MQ=255)

                                                                                                                     cATGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAAtt                      >  2:6721216/1‑140 (MQ=255)

                                                                                                                      aTGAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAACTACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTg                     <  2:10920656/140‑1 (MQ=255)

                                                                                                                        gAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGAc                   <  1:4990662/140‑1 (MQ=255)

                                                                                                                        gAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGAc                   >  2:5893274/1‑140 (MQ=255)

                                                                                                                        gAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGAc                   <  1:2977655/140‑1 (MQ=255)

                                                                                                                        gAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGAc                   <  1:1465354/140‑1 (MQ=255)

                                                                                                                        gAGCGGCGCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGAGTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGAc                   <  1:4552745/140‑1 (MQ=255)

                                                                                                                        gAGCGGCGCTTATGCGACGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCATGTTAAAAAATGATTTCCTGCGTTACTAAATCCCTTGTGCTCAATTGAc                   <  2:2910367/140‑1 (MQ=255)

                                                                                                                        ccgcggccCTTTGGCGCCGC‑TTATTT‑A‑T‑CCG‑ACACACCTTAATTACATCCCCATAGCTTAATTGCGTCAGCTCACAAATACGCTTATACCCAGGTAAAAAAAGAATTCCTGCGAGACTAAAACCCTTGTGCTCAATTGAc                   <  2:10038459/138‑1 (MQ=255)

                                                                                                                           cggcgCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTCCAGCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATGTCCTGCGTGACTACAACCCTTGTGCTCAATTGACAGt                <  1:2503417/140‑1 (MQ=255)

                                                                                                                           cggcgCTTATGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGt                <  1:9328248/140‑1 (MQ=255)

                                                                                                                                   aTGCGCCGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTACATACCCATATATTTTTTGCGTCAGCTCACAAATACGCATTTTCCCTGGTAAAAAATGATTTACAGCGTGACTAAAACCCTTGTGCTCAATTTACAGTTTAtttta        >  1:7611783/1‑139 (MQ=255)

                                                                                                                                     gcgcCGT‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTg      <  1:6111246/140‑1 (MQ=255)

GACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTTT‑T‑T‑CTG‑TCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCGGA  >  NZ_CP025268/1374716‑1374991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`