BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,660,178	+C	coding (314/351 nt)	ynfA ←	YnfA family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,660,174	1	.	C	98.7%	1849.0 / 13.0	539	coding (318/351 nt)	ynfA	YnfA family protein
Reads supporting (aligned to +/- strand): ref base . (5/2); new base C (252/278); total (259/280)
Fisher's exact test for biased strand distribution p-value = 2.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 570 total aligned reads displayed.

CGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCG‑CC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCATAAGC  >  NZ_CP025268/1660036‑1660308

cGAAAACAGCCGGAGTGCTCACAAAACAAGGCGTAGAGTAGCGCTGAGAATGGTAGACAAAAGAGGAAAGAGCATACCATTTGTCAGCTGACACTTCCTCTTATCGTATTGATAAAAGGGATTTATGGTCCTACGTgcg‑c                                                                                                                                         <  2:3741975/140‑1 (MQ=255)

cGAAAAAAGCCGGAGTTATCACAAAAAAAGGCTTATATTTGCGCTGTGAAGGGTTGACAAAATATGAAATAGAATACATTTTGTCAGCTGACACTTAATCTTATCTTATTGATAAAATGGATTTATTTTCCTAAGTgcg‑c                                                                                                                                         >  2:4745602/1‑140 (MQ=255)

cGAAAAAAGCCGGAGTGATCACAAAAAAGGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTgcg‑c                                                                                                                                         >  2:5985197/1‑140 (MQ=255)

cGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATTGATTTATGTTCCTACGTgcg‑c                                                                                                                                         >  2:2038667/1‑140 (MQ=255)

cGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTgcg‑c                                                                                                                                         >  1:6510240/1‑140 (MQ=255)

cGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTgcg‑c                                                                                                                                         >  1:2499456/1‑140 (MQ=255)

   aaaaaGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑cc‑c‑c                                                                                                                                    <  2:7406633/140‑1 (MQ=255)

     aaaGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑cc‑c‑cca                                                                                                                                  <  1:3458935/140‑3 (MQ=255)

          cGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCg                                                                                                                              <  1:9929641/140‑1 (MQ=255)

          cGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCg                                                                                                                              <  1:10484380/140‑1 (MQ=255)

               tGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGcaaca                                                                                                                         <  1:7906068/140‑1 (MQ=255)

               tGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGcaaca                                                                                                                         <  1:3067243/140‑1 (MQ=255)

                 aTCACAAAAAAAGGCGTATATTTTCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTATTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAAt                                                                                                                       >  2:2455968/1‑140 (MQ=255)

                      aaaaaaaGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGACATAGACTCCCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCa                                                                                                                  <  1:568647/140‑1 (MQ=255)

                       aaaaaaGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCaa                                                                                                                 >  1:4609610/1‑140 (MQ=255)

                           aaGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAAGGATCAACATg                                                                                                             >  1:8831715/1‑140 (MQ=255)

                               cGTATATTTGCGCTGTGAATGGTTGACAACAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGc                                                                                                         <  2:2578662/140‑1 (MQ=255)

                               cGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGc                                                                                                         <  2:2985197/140‑1 (MQ=255)

                                gTATATTTGCTCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCa                                                                                                        <  2:1640640/140‑1 (MQ=255)

                                gTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCa                                                                                                        >  2:10836486/1‑140 (MQ=255)

                                gTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCa                                                                                                        <  2:3110119/140‑1 (MQ=255)

                                        gcgcTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCaa                                                                                                >  1:3640303/1‑140 (MQ=255)

                                        gcgcTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCaa                                                                                                >  2:6789062/1‑140 (MQ=255)

                                             gtgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAAc                                                                                           >  1:4240315/1‑140 (MQ=255)

                                              tgAATGGTTGACAAAAGATGAAATAGAATAGCTTTTGTCAGCTGACTCTTCCTCGTATCTTATTGTGAAAGTGGTTTTTTGTTCATACGTGCGCCC‑C‑CAGACCGCAACAATGAGCATCTTGCCGCGAAGCGCAAGCATCg                                                                                          >  2:10563386/1‑140 (MQ=255)

                                              tgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTTATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACg                                                                                          >  2:9060726/1‑140 (MQ=255)

                                               gAATTGTTGACAAAAGATGAAATAGAATAACTTTTTTCATCTGACACTCCCTATTATCTTATTGATTAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACTATGATCAACATGCCGCAAAGCGCAATCAACGc                                                                                         <  2:8186270/140‑1 (MQ=255)

                                               gAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGc                                                                                         >  2:8782174/1‑140 (MQ=255)

                                                aaTGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCa                                                                                        >  1:4800722/1‑140 (MQ=255)

                                                   ggTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCAccc                                                                                     >  1:7491691/1‑140 (MQ=255)

                                                      tGACAAAAGATGAAATAGACTACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                                  <  1:6403022/140‑1 (MQ=255)

                                                      tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                                  <  1:6913376/140‑1 (MQ=255)

                                                      tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                                  <  1:919274/140‑1 (MQ=255)

                                                      tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                                  <  1:7829374/140‑1 (MQ=255)

                                                      tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                                  >  1:3134836/1‑140 (MQ=255)

                                                      tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                                  <  1:1526363/140‑1 (MQ=255)

                                                      tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                                  <  1:10803739/140‑1 (MQ=255)

                                                       gACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTcc                                                                                 >  2:4004370/1‑140 (MQ=255)

                                                        aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATCAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                                >  1:6841486/1‑140 (MQ=255)

                                                        aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGcaaca                                                                                                                         >  2:10699463/1‑99 (MQ=255)

                                                        aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                                >  1:5118110/1‑140 (MQ=255)

                                                        aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                                >  1:3876347/1‑140 (MQ=255)

                                                        aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                                <  1:33288/140‑1 (MQ=255)

                                                        aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATGTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                                >  2:6196217/1‑140 (MQ=255)

                                                          aaaaGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGt                                                                              <  2:5662240/140‑1 (MQ=255)

                                                               aTGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATaa                                                                         >  1:4070352/1‑140 (MQ=255)

                                                                tGAAATAGACTACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATaaa                                                                        <  2:3533413/140‑1 (MQ=255)

                                                                    aTAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGt                                                                    <  1:11128563/140‑1 (MQ=255)

                                                                           aCCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTc                                                             <  1:10295544/140‑1 (MQ=255)

                                                                              ttttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACg                                                          <  2:4355272/140‑1 (MQ=255)

                                                                               tttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGc                                                         >  1:138782/1‑140 (MQ=255)

                                                                                     aGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCca                                                   <  1:1767612/140‑1 (MQ=255)

                                                                                      gCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCca                                                   <  2:3215108/139‑1 (MQ=255)

                                                                                      gCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGGTTCACGCCATCcac                                                  >  2:8562430/1‑140 (MQ=255)

                                                                                       cTGACACTTCCTCTTCTCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATACAGAGTCAGTTTCACGCCATCcaca                                                 <  1:902429/140‑1 (MQ=255)

                                                                                       cTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCcaca                                                 >  2:1303384/1‑140 (MQ=255)

                                                                                       cTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCcaca                                                 >  2:1708532/1‑140 (MQ=255)

                                                                                       cTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATC‑ACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACaa                                                >  2:4603704/1‑140 (MQ=255)

                                                                                          acacTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAAcg                                              <  2:5819486/140‑1 (MQ=255)

                                                                                          acacTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAAcg                                              >  1:11401619/1‑140 (MQ=255)

                                                                                           cacTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAAcgc                                             <  1:7306275/140‑1 (MQ=255)

                                                                                             cTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCa                                                                                        >  2:6911768/1‑95 (MQ=255)

                                                                                                ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAACGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                        <  1:10694431/140‑1 (MQ=255)

                                                                                                  tcttatcttaTTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCca                                      <  1:5475335/140‑1 (MQ=255)

                                                                                                     tatcttatTGATAAAATGGATTTATGTTCCTACGTGCG‑CC‑CACAGCCCGCAACAATGAACAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTATCACGCCATCCACAACGCGCAGCCACAt                                   >  1:2858656/1‑140 (MQ=255)

                                                                                                      atcttatTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATc                                  >  1:5370503/1‑140 (MQ=255)

                                                                                                      atcttatTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATc                                  >  2:7514018/1‑140 (MQ=255)

                                                                                                        cttatTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCaa                                >  2:6447687/1‑140 (MQ=255)

                                                                                                          tatTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACg                              >  1:8550482/1‑140 (MQ=255)

                                                                                                          tatTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACg                              >  2:10395958/1‑140 (MQ=255)

                                                                                                          gatTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCACTCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACg                              <  1:6638098/139‑1 (MQ=255)

                                                                                                           atTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGc                             >  1:6216364/1‑140 (MQ=255)

                                                                                                           atTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGc                             >  2:10183721/1‑140 (MQ=255)

                                                                                                           atTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGc                             <  1:6757114/140‑1 (MQ=255)

                                                                                                           atTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGc                             >  1:5905354/1‑140 (MQ=255)

                                                                                                           atTGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTAATAAAGAGTCAGTTTCACTCCATCCACAACGCGCAGCCACATCAACGc                             >  2:1884577/1‑140 (MQ=255)

                                                                                                            ttGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGcc                            >  1:2231474/1‑140 (MQ=255)

                                                                                                            ttGATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGcc                            >  2:6629961/1‑140 (MQ=255)

                                                                                                              gATAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGt                          >  2:11459063/1‑140 (MQ=255)

                                                                                                               atatAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCCCAACGCGCAGCCACATCAACGCCGTg                         <  1:807045/140‑1 (MQ=255)

                                                                                                               aTAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTg                         <  2:2665184/140‑1 (MQ=255)

                                                                                                                tAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGc                        <  1:9731054/140‑1 (MQ=255)

                                                                                                                tAAAATGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGc                        >  1:10037168/1‑140 (MQ=255)

                                                                                                                 aaaaTGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCa                       <  1:11005388/140‑1 (MQ=255)

                                                                                                                 aaaaTGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCa                       <  2:8313681/140‑1 (MQ=255)

                                                                                                                    aTGGATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGAc                    <  2:5091268/140‑1 (MQ=255)

                                                                                                                      ggATTTATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACAt                  <  2:9717563/140‑1 (MQ=255)

                                                                                                                         tttATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATaaa               <  2:11012027/140‑1 (MQ=255)

                                                                                                                         tttATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATaaa               >  1:9459575/1‑140 (MQ=255)

                                                                                                                           tctGTTCCTCCGGGCGCCC‑C‑CAGCCCGCCACACGGCGCAACCTGCCGCAAAGCGCACTCAACGCACCCGTCCCGGCCGAAAGAGTCAGGTTCACGCCATCCACAACGCGCCGCCACATCAACGCCGTGCAGACATAACCg             <  2:531018/138‑1 (MQ=255)

                                                                                                                           tATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCCGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACg             <  1:208160/140‑1 (MQ=255)

                                                                                                                           tATGTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACg             <  1:8317515/140‑1 (MQ=255)

                                                                                                                              gTTCCTACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGcca          >  2:9920415/1‑140 (MQ=255)

                                                                                                                                 cctACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGccacca       >  1:10886718/3‑140 (MQ=255)

                                                                                                                                 cctACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGccacca       >  2:9303996/3‑140 (MQ=255)

                                                                                                                                 cctACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCAGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGTGTCAGTTTCACGCCATCCACAACGCGCTGCCACATCAACGCCGTGCAGACATAAACGccacca       >  2:2148745/3‑140 (MQ=255)

                                                                                                                                  ctACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCAt      >  1:4336389/2‑140 (MQ=255)

                                                                                                                                  ctACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCAt      >  2:7921765/2‑140 (MQ=255)

                                                                                                                                  ctACGTGCGCCC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCAt      <  2:9550665/139‑1 (MQ=255)

                                                                                                                                     cGTGCG‑CCAC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCATAAg   <  2:4838824/140‑1 (MQ=255)

CGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCG‑CC‑C‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCATAAGC  >  NZ_CP025268/1660036‑1660308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`