BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,873,836	(T)_5→6	pseudogene (67/1283 nt)	yeaH →	YeaH/YhbH family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,873,831	1	.	T	94.7%	1863.9 / 83.1	528	pseudogene (62/1283 nt)	yeaH	YeaH/YhbH family protein
Reads supporting (aligned to +/- strand): ref base . (13/15); new base T (301/196); total (315/213)
Fisher's exact test for biased strand distribution p-value = 1.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 597 total aligned reads displayed.

CAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTTCATCAGG  >  NZ_CP025268/1873692‑1873967

    gACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑t‑t‑t‑t                                                                                                                                      >  2:34411/1‑140 (MQ=255)

      cAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑t‑t‑t‑ttt                                                                                                                                    >  1:6919243/1‑139 (MQ=255)

       cgCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATAAAGTTGGCAAATGTAGTACGGGGGGCATATGACCTTGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑t‑t‑t‑ttta                                                                                                                                   <  2:8686601/139‑3 (MQ=255)

       aGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑t‑t‑t‑ttta                                                                                                                                   <  1:6557446/140‑3 (MQ=255)

       aGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑t‑t‑t‑ttta                                                                                                                                   <  1:6176035/140‑3 (MQ=255)

       aGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑t‑t‑t‑ttta                                                                                                                                   <  1:4622960/140‑3 (MQ=255)

         cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑t‑t‑t‑tttacg                                                                                                                                 <  2:9460375/140‑5 (MQ=255)

         cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑t‑t‑t‑tttacg                                                                                                                                 <  1:7352167/140‑5 (MQ=255)

         cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑t‑t‑t‑tttacg                                                                                                                                 <  1:9639940/140‑5 (MQ=255)

         cGAACCGTGGGCCAGAGAAGCGGCAACACAGGCGTAGCATACAGTAGGCAAATGAAGTACGGGGGGCATATGACCTGGATAATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAAACGCCAGCG‑T‑T‑TATTACg                                                                                                                                  >  1:10956200/1‑140 (MQ=255)

                tGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTtat                                                                                                                           <  2:7333169/140‑1 (MQ=255)

                tGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGGtat                                                                                                                           >  1:10224626/1‑140 (MQ=255)

                 gggCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTtata                                                                                                                          >  1:9168078/1‑140 (MQ=255)

                     cTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAgc                                                                                                                      <  1:829965/140‑1 (MQ=255)

                     cTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAgc                                                                                                                      <  1:1542947/140‑1 (MQ=255)

                        agaAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCa                                                                                                                   >  2:2518743/1‑140 (MQ=255)

                             cGGCAACACAGGCGTAGCATACAGTTGGCAAATGTCGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTa                                                                                                              >  2:7521133/1‑140 (MQ=255)

                             cGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAAtt                                                                                                               >  2:10468177/1‑139 (MQ=255)

                             cGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTa                                                                                                              >  2:9723836/1‑140 (MQ=255)

                             cGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATGGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTa                                                                                                              <  2:7635080/140‑1 (MQ=255)

                               gCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTaaa                                                                                                            <  1:747082/140‑1 (MQ=255)

                                  acacAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAg                                                                                                         >  2:3233394/1‑140 (MQ=255)

                                   cacaGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGt                                                                                                        <  2:9355126/140‑1 (MQ=255)

                                    acaGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTc                                                                                                       <  1:2868318/140‑1 (MQ=255)

                                               aTACAGTTGGCAAATGTAGTACGGGTGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                            <  1:8866983/140‑1 (MQ=255)

                                               aTACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                            <  2:1380025/140‑1 (MQ=255)

                                                tACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCGAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGc                                                                                           >  2:9205850/1‑140 (MQ=255)

                                                tACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGc                                                                                           >  2:6755597/1‑140 (MQ=255)

                                                tACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGc                                                                                           <  1:1257474/140‑1 (MQ=255)

                                                tACAGTTGGCAAATGTAGTACGGGGGGAATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGAGCAAATTAAACAGTCGATCTCCGAGGc                                                                                           >  2:4241158/1‑140 (MQ=255)

                                                  cAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCa                                                                                         >  2:8504455/1‑140 (MQ=255)

                                                  cAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCa                                                                                         >  2:1668315/1‑140 (MQ=255)

                                                  cAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCa                                                                                         >  1:79834/1‑140 (MQ=255)

                                                  cAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCa                                                                                         >  1:2507861/1‑140 (MQ=255)

                                                   aGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCAt                                                                                        >  2:7744327/1‑140 (MQ=255)

                                                    gTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCAtt                                                                                       <  2:9836199/140‑1 (MQ=255)

                                                      tGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGACGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaa                                                                                     >  2:4805389/1‑140 (MQ=255)

                                                       ggCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaat                                                                                    >  1:10406427/1‑140 (MQ=255)

                                                       ggCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaat                                                                                    >  1:5953488/1‑140 (MQ=255)

                                                       ggCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaat                                                                                    >  1:9927363/1‑140 (MQ=255)

                                                       ggCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaat                                                                                    >  1:5302571/1‑140 (MQ=255)

                                                       ggCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCAGtaat                                                                                    >  1:3150764/1‑140 (MQ=255)

                                                        gCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaata                                                                                   >  2:4504601/1‑140 (MQ=255)

                                                        gCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaata                                                                                   >  2:7201957/1‑140 (MQ=255)

                                                        gCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaata                                                                                   >  1:3277122/1‑140 (MQ=255)

                                                             tGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGt                                                                              >  2:567414/1‑140 (MQ=255)

                                                             tGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCAAGGCCATTAATAAGCGt                                                                              >  1:481358/1‑140 (MQ=255)

                                                              gtagtaCGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGtt                                                                             <  1:5071188/140‑1 (MQ=255)

                                                               tagtaCGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTc                                                                            >  2:2375387/1‑140 (MQ=255)

                                                                agtaCGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCg                                                                           <  1:4350493/140‑1 (MQ=255)

                                                                  taCGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGt                                                                         >  1:3041213/1‑140 (MQ=255)

                                                                    cGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGCTCTCCGAGGCCATTAATAAGCGTTCGGtga                                                                       <  2:7944997/140‑1 (MQ=255)

                                                                    cGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtga                                                                       <  1:1943002/140‑1 (MQ=255)

                                                                    cGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtga                                                                       <  1:7259068/140‑1 (MQ=255)

                                                                       ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactg                                                                    >  2:5006231/1‑140 (MQ=255)

                                                                       ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactg                                                                    >  1:2164523/1‑140 (MQ=255)

                                                                       ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactg                                                                    >  1:363065/1‑140 (MQ=255)

                                                                       ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactg                                                                    >  2:10998165/1‑140 (MQ=255)

                                                                         ggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactgac                                                                  >  2:6097783/1‑140 (MQ=255)

                                                                         ggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATAAATAAGCGTTCGGtgactgac                                                                  >  2:7004617/1‑140 (MQ=255)

                                                                            atatGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTc                                                               >  1:3950965/1‑140 (MQ=255)

                                                                              atGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TAT‑T‑TTACGCCGTTATAAAGCTCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTAGGTGACTGACGTCGa                                                             >  2:8970548/1‑140 (MQ=255)

                                                                                gACCTGGTTTATTGACCGGCGTCTGAACGGCAATATCACAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGACGTTATAAAGCGCAAATTAAACAGTCGATCTACGCGGCCAGTAATACGCGTTAGGTGACTGACGTCGACa                                                           >  2:496337/1‑140 (MQ=255)

                                                                                 aCCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAg                                                          >  1:7106754/1‑140 (MQ=255)

                                                                                   cTGGTTTAGTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTGATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcg                                                        >  2:6278924/1‑140 (MQ=255)

                                                                                     ggTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggc                                                      >  2:11155126/1‑140 (MQ=255)

                                                                                     ggTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggc                                                      >  2:3922519/1‑140 (MQ=255)

                                                                                      gTTTATTGACCGGCGTCTGAACGGCAAACACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggcg                                                     <  1:10711274/140‑1 (MQ=255)

                                                                                      gTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggcg                                                     >  1:1705381/1‑140 (MQ=255)

                                                                                      gTTTATTGACCGGCGTCTGAACCGCAAAAACAAAAGCATGTTGAATCGCCAGCG‑T‑TGT‑TTACGCCTATATAAAGCGCAAATTAAACAGTCGATCTCCGCGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggcg                                                     <  2:1132626/140‑1 (MQ=255)

                                                                                         tATTGACCGGCGTCTGAACGGCAAAAACAAAAGAATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGTCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAAt                                                  >  1:7636470/1‑140 (MQ=255)

                                                                                          aTTGACCGACGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATc                                                 <  2:1474732/140‑1 (MQ=255)

                                                                                           ttGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATcc                                                >  2:5205985/1‑140 (MQ=255)

                                                                                           ttGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATcc                                                >  1:9343340/1‑140 (MQ=255)

                                                                                            tGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCg                                               >  2:10213409/1‑140 (MQ=255)

                                                                                                cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATc                                           >  1:5709731/1‑140 (MQ=255)

                                                                                                cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATc                                           >  2:2884417/1‑140 (MQ=255)

                                                                                                cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATc                                           >  1:6399942/1‑140 (MQ=255)

                                                                                                   cGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCAt                                        >  1:549596/1‑140 (MQ=255)

                                                                                                     acTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTc                                      >  1:4163183/2‑140 (MQ=255)

                                                                                                        gAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCa                                   <  1:10027152/140‑1 (MQ=255)

                                                                                                        gAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCa                                   <  1:3361407/140‑1 (MQ=255)

                                                                                                        gAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTAGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGTCGTCGACAGCGGCGAATCCGTATCCATTCCCa                                   <  1:8633355/140‑1 (MQ=255)

                                                                                                          aCGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACg                                 <  2:5935533/140‑1 (MQ=255)

                                                                                                          aCGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACg                                 <  2:3054602/140‑1 (MQ=255)

                                                                                                          aCGGCAAAAACAAAAGCAGGGTGAATCGCCAGCG‑TAT‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACg                                 <  2:8287466/140‑1 (MQ=255)

                                                                                                            ggCAAAAACAATAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCTAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGa                               <  2:367008/140‑1 (MQ=255)

                                                                                                             gCAAAAACAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCAGTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGaa                              >  2:2031288/1‑140 (MQ=255)

                                                                                                               aaaaaCATAAGCAGGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATCACGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGa                            <  2:1212881/140‑1 (MQ=255)

                                                                                                               aaaaaCAAAAGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGa                            <  1:9061110/140‑1 (MQ=255)

                                                                                                                      aaaGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGc                     >  2:6573811/1‑140 (MQ=255)

                                                                                                                      aaaGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCACACGGAAGATATTAGc                     >  2:10659100/1‑140 (MQ=255)

                                                                                                                       aaGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCg                    >  2:8407510/1‑140 (MQ=255)

                                                                                                                        aGCATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGa                   >  2:1930637/1‑140 (MQ=255)

                                                                                                                          cATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAAc                 <  1:2674489/140‑1 (MQ=255)

                                                                                                                          cATGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAAc                 <  1:11140404/140‑1 (MQ=255)

                                                                                                                           aTGGTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAAcc                <  1:11300200/140‑1 (MQ=255)

                                                                                                                              gTGAATCGCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGAt             <  1:4965210/140‑1 (MQ=255)

                                                                                                                                gAAACGCCAGCG‑TAT‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGt           <  1:3815638/140‑1 (MQ=255)

                                                                                                                                     gCCAGCGTT‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTtcat      <  1:7684702/140‑1 (MQ=255)

CAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑T‑T‑T‑TTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTTCATCAGG  >  NZ_CP025268/1873692‑1873967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`