BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,218,374	(C)_5→6	pseudogene (306/1685 nt)	btsS ←	two‑component regulatory system sensor histidine kinase BtsS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,218,369	1	.	C	98.2%	2124.5 / 24.9	617	pseudogene (311/1685 nt)	btsS	two‑component regulatory system sensor histidine kinase BtsS
Reads supporting (aligned to +/- strand): ref base . (7/4); new base C (327/278); total (335/282)
Fisher's exact test for biased strand distribution p-value = 7.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 670 total aligned reads displayed.

CAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑C‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCAACCCAAAC  >  NZ_CP025268/2218230‑2218503

cAGCGACGAACGTGACGGCACCGGCGATAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATg                                                                                                                                            <  1:6123285/140‑1 (MQ=255)

 aGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑c                                                                                                                                          <  1:4287492/140‑1 (MQ=255)

  gagtcgaCCGTGACGGCTGCGGCGGTTTTGGGGTGAAAGACTTTCTCAGTGCGCCCGCGGCGGATATGGTTGCTGTGTACCTGGCAACCGTGTAATCATTCAACGATGGTCGAGATCATGCATCTTAACGCGGTCATgag                                                                                                                                          <  2:7722270/136‑3 (MQ=255)

  gCGACGCACGTGACGGCACCGGCGGGAATGGGGTTAACGACTTTCTCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑c‑c                                                                                                                                        <  2:10060697/140‑1 (MQ=255)

  gCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑c‑c                                                                                                                                        <  1:3650069/140‑1 (MQ=255)

  gCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑c‑c                                                                                                                                        <  2:5321570/140‑1 (MQ=255)

    gacgaACGTGACGGCACCGGCGGTAATGGTGTTAAAGACTTGATCAGTTCGCCCGCGGCGGATCAGGATGCGGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑c‑cc‑c                                                                                                                                     <  2:549156/140‑1 (MQ=255)

                       gcggTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTaa                                                                                                                  >  1:9429372/1‑140 (MQ=255)

                           tAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAgccg                                                                                                              <  1:11388316/140‑1 (MQ=255)

                            aaTGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAgccgc                                                                                                             >  1:6328371/1‑140 (MQ=255)

                                gggTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGt                                                                                                         <  1:7366689/140‑1 (MQ=255)

                                 ggTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTc                                                                                                        >  1:1661418/1‑140 (MQ=255)

                                 ggTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTc                                                                                                        >  2:8941438/1‑140 (MQ=255)

                                    tAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGc                                                                                                     <  1:4934617/140‑1 (MQ=255)

                                     aaaGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCNACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGcc                                                                                                    >  1:10622218/1‑140 (MQ=255)

                                     aaaGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGcc                                                                                                    >  2:8344101/1‑140 (MQ=255)

                                         aCTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑CTCGAATATCGATGTAAGCCGCCGGTCAGCCCAAc                                                                                                <  1:10200173/140‑1 (MQ=255)

                                         aCTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAAc                                                                                                <  1:204915/140‑1 (MQ=255)

                                         aCTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAAc                                                                                                <  1:8678410/140‑1 (MQ=255)

                                         aCTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAAc                                                                                                <  1:4434458/140‑1 (MQ=255)

                                             tATCAGTGCGCCCGCGGCGGATCCGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTCACGCGGTCATGCC‑CC‑CC‑ATCGACTCTCGATGGAAGCCGCCGGTCAGCGCTCCCAGc                                                                                            >  1:8230261/1‑140 (MQ=255)

                                                   tGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACcg                                                                                      >  2:634795/1‑140 (MQ=255)

                                                   tGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACcg                                                                                      >  2:11231512/1‑140 (MQ=255)

                                                    gcgcCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACcga                                                                                     >  2:11463270/1‑140 (MQ=255)

                                                     cgcCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCTATGTAAGCCGCCGGTCAGCCCAACCAGCCCACcgac                                                                                    >  1:2420166/1‑140 (MQ=255)

                                                     cgcCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACcgac                                                                                    >  1:2718065/1‑140 (MQ=255)

                                                       cccGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACcgacga                                                                                  >  1:5793746/1‑140 (MQ=255)

                                                            ggcggATCATGATGCTGTGTACCAGGCCAACGAGTAATCCTTCAACGATGGTCGAGATCATGCCACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACgaccgga                                                                             <  2:9128368/140‑1 (MQ=255)

                                                              cggATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACgaccggacc                                                                           <  1:3279253/140‑1 (MQ=255)

                                                                  tCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGAccgccg                                                                       >  2:5117530/1‑140 (MQ=255)

                                                                        tGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGAGCGGACCGCCGAGTAAg                                                                 <  1:1826649/140‑1 (MQ=255)

                                                                        tGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAg                                                                 <  1:210183/140‑1 (MQ=255)

                                                                        tGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAg                                                                 >  1:1404869/1‑140 (MQ=255)

                                                                        tGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAg                                                                 <  2:651668/140‑1 (MQ=255)

                                                                        tGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAg                                                                 <  2:4242718/140‑1 (MQ=255)

                                                                        tGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAg                                                                 <  2:8528525/140‑1 (MQ=255)

                                                                         gCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAgc                                                                <  1:9055536/140‑1 (MQ=255)

                                                                             tgtACCTTGACACCGATTAATCCTTTAACGATGGTCTGTATCATGCATCTTAACGCTGTCATG‑CTCC‑CC‑ATCGTATATCGATGTAAGCCGCCGTTCAGCCCAACCAGCCCACCGTCGACCGGTCCGCCGAGTAAgccgcc                                                            <  2:4628963/140‑1 (MQ=255)

                                                                             tgtACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑CTCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAgccgcc                                                            <  1:10959788/140‑1 (MQ=255)

                                                                             tgtACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAgccgcc                                                            <  1:10343834/140‑1 (MQ=255)

                                                                             tgtACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑AGCGAATATCGATGTAAGCCGCCGGTCAGCCCCACCAGCCCACCGACGACCGGACCGCCGAGTCAgccgcc                                                            >  1:8359577/1‑140 (MQ=255)

                                                                             tgtACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGNCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTACgccgcc                                                            >  1:6049691/1‑140 (MQ=255)

                                                                               tACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGAGGTCATG‑C‑CC‑CCAATAGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGACGCCCa                                                          >  2:10308181/1‑140 (MQ=255)

                                                                                aCCAGGCCACCGAGTCCTCCTGCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCACCCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCAt                                                         >  2:2124763/1‑140 (MQ=255)

                                                                                aCCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCAt                                                         >  1:5245649/1‑140 (MQ=255)

                                                                                   aGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTCAGCCGCCCATTAc                                                      <  1:9817534/140‑1 (MQ=255)

                                                                                    ggCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTAcc                                                     >  1:7504782/1‑140 (MQ=255)

                                                                                    ggCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTAcc                                                     >  2:9452900/1‑140 (MQ=255)

                                                                                     gCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACcg                                                    >  1:5096940/1‑140 (MQ=255)

                                                                                       cACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACcgcg                                                  >  1:4493685/1‑140 (MQ=255)

                                                                                       cACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACcgcg                                                  >  2:1327191/1‑140 (MQ=255)

                                                                                       cACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACcgcg                                                  >  2:10907499/1‑140 (MQ=255)

                                                                                       cACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACcgcg                                                  >  2:3513254/1‑140 (MQ=255)

                                                                                       cACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACcgcg                                                  >  1:8071016/1‑140 (MQ=255)

                                                                                         ccGAGTAATCCTTCACCGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGcc                                                <  2:3030911/140‑1 (MQ=255)

                                                                                         ccGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGcc                                                >  1:6552040/1‑140 (MQ=255)

                                                                                           gAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTa                                              <  1:2612646/140‑1 (MQ=255)

                                                                                              tAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCg                                           <  2:2916409/140‑1 (MQ=255)

                                                                                               aaTCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGc                                          <  2:2586822/140‑1 (MQ=255)

                                                                                                aTCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAGGCCGCCCATTACCGCGCCTATCGCa                                         <  2:2312081/140‑1 (MQ=255)

                                                                                                aTCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa                                         <  2:3230669/140‑1 (MQ=255)

                                                                                                aTCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa                                         <  2:4025589/140‑1 (MQ=255)

                                                                                                aTCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa                                         <  1:4005732/140‑1 (MQ=255)

                                                                                                aTCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa                                         <  2:6206654/140‑1 (MQ=255)

                                                                                                aTCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa                                         <  2:191008/140‑1 (MQ=255)

                                                                                                aTCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa                                         <  1:11246352/140‑1 (MQ=255)

                                                                                                aTCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa                                         <  2:1200446/140‑1 (MQ=255)

                                                                                                aTCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa                                         <  2:5703691/140‑1 (MQ=255)

                                                                                                aTCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa                                         <  1:7747718/140‑1 (MQ=255)

                                                                                                aTCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa                                         <  1:6721259/140‑1 (MQ=255)

                                                                                                 tCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCAc                                        <  2:8734138/140‑1 (MQ=255)

                                                                                                     tCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGt                                    <  2:6732154/140‑1 (MQ=255)

                                                                                                      cAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTa                                   >  2:6040451/1‑140 (MQ=255)

                                                                                                      cAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTa                                   >  1:15716/1‑140 (MQ=255)

                                                                                                       aaCGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑C‑CCACC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGTCCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTAt                                  <  2:10778361/140‑1 (MQ=255)

                                                                                                        aCGATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTCtt                                 >  2:7015071/1‑140 (MQ=255)

                                                                                                          gATGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTgg                               <  1:5462992/140‑1 (MQ=255)

                                                                                                            tGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCa                             >  2:1710430/1‑140 (MQ=255)

                                                                                                            tGGTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCa                             >  1:475070/1‑140 (MQ=255)

                                                                                                              gTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAAt                           <  2:8101231/140‑1 (MQ=255)

                                                                                                              gTCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAAt                           >  1:5652673/1‑140 (MQ=255)

                                                                                                               tCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATa                          >  1:6857635/1‑140 (MQ=255)

                                                                                                               tCGAGATCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCAGCCGGTCATCCCAACCAGCACACCGACGACCGGACAGCCGAGTAAGCCGCCCATTACCGCGCCTCTCGCACGGGTCTTGGCAATa                          >  2:9271666/1‑140 (MQ=255)

                                                                                                                  agaTCCTGCACCTTACCGCGGTCCTGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCAGTACCGCGCCTATCGCACGGGTATTGGCAATAGaa                       <  2:2725227/140‑1 (MQ=255)

                                                                                                                   gaTCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAAt                      <  2:491930/140‑1 (MQ=255)

                                                                                                                   gaTCATGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAAt                      <  1:1235954/140‑1 (MQ=255)

                                                                                                                       aTGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTc                  <  2:1061075/140‑1 (MQ=255)

                                                                                                                        tGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGa                        >  2:9688605/1‑133 (MQ=255)

                                                                                                                        tGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCa                 >  2:5033828/1‑140 (MQ=255)

                                                                                                                        tGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCa                 <  2:2403969/140‑1 (MQ=255)

                                                                                                                        tGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCa                 >  1:9330927/1‑140 (MQ=255)

                                                                                                                        tGCAACTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCa                 >  1:4144560/1‑140 (MQ=255)

                                                                                                                             cTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAAtgtg            >  1:10488874/1‑140 (MQ=255)

                                                                                                                             cTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAAtgtg            <  2:4450877/140‑1 (MQ=255)

                                                                                                                             cTTAACGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAAtgtg            >  1:2945222/1‑140 (MQ=255)

                                                                                                                                aaCGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGACTCGTCAATGTGCaa         <  2:3661177/140‑1 (MQ=255)

                                                                                                                                aaCGCGGTCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCaa         >  1:1002176/1‑140 (MQ=255)

                                                                                                                                       tCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCAACCCAAAc  >  2:74161/1‑140 (MQ=255)

                                                                                                                                       tCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCAACCCAAAc  >  2:7964310/1‑140 (MQ=255)

                                                                                                                                       tCATGCC‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCAACCCAAAc  >  2:586991/1‑140 (MQ=255)

CAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATG‑C‑CC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCAACCCAAAC  >  NZ_CP025268/2218230‑2218503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`