BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,292,596	(C)_6→7	pseudogene (361/2531 nt)	yejO ←	autotransporter YejO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,292,590	1	.	C	97.4%	1836.0 / 35.5	542	pseudogene (367/2531 nt)	yejO	autotransporter YejO
Reads supporting (aligned to +/- strand): ref base . (10/4); new base C (205/321); total (216/326)
Fisher's exact test for biased strand distribution p-value = 2.35e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 579 total aligned reads displayed.

ACCGAGACGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑CCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCG  >  NZ_CP025268/2292451‑2292723

aCCGAGACGGTTTGTTCCGAATACCTCCATTGCCCGCGTGGTTGTTTTAATAGCTCCGCCTGCTTTCTGAGCTACCGCAACTGCAAATCCCCCCTCTTTCACCTCCAGTAGTCCTCCCTGGTCGATCGCGACACTGACgg                                                                                                                                         >  2:9433780/1‑140 (MQ=255)

aCCGAGACGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACgg                                                                                                                                         >  1:185554/1‑140 (MQ=255)

      aCGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑ccc‑ccc                                                                                                                                 >  2:9651099/1‑140 (MQ=255)

      aCGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑ccc‑ccc                                                                                                                                 >  1:10998974/1‑140 (MQ=255)

          tttGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑ccc‑ccccatc                                                                                                                             >  2:4245443/1‑136 (MQ=255)

               tCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCttttt                                                                                                                         >  1:9474909/1‑140 (MQ=255)

                 cGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATCGCGACAAAGACGGCCCC‑CCCATCTTTTTCa                                                                                                                       <  2:3168934/140‑1 (MQ=255)

                 cGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCTACAAAGACGGCCCC‑CCCATCTTTTTCa                                                                                                                       <  2:2525727/140‑1 (MQ=255)

                          ccATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAg                                                                                                              <  1:1451177/140‑1 (MQ=255)

                             tGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTGTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAgcgc                                                                                                           >  1:8629518/1‑140 (MQ=255)

                              ggCCCGCGTGGTTGTTTTCATAGCACCGCCTGCGTTCTGATCTCCCGCAACTGCACAGCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTGGTCGATAGCGACACAGACGGCCCC‑CCCATCTTTTTCACCGATTAAGcgcc                                                                                                          <  2:7740932/140‑1 (MQ=255)

                              ggCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGcgcc                                                                                                          <  2:6450591/140‑1 (MQ=255)

                              ggCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGcgcc                                                                                                          >  2:11329999/1‑140 (MQ=255)

                               gCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAATACGGCCCC‑CCCATCTTTTACAACGATAAAGcgccc                                                                                                         >  1:1270426/1‑140 (MQ=255)

                               gCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGcgccc                                                                                                         <  1:11122206/140‑1 (MQ=255)

                               gCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGcgccc                                                                                                         <  1:6773287/140‑1 (MQ=255)

                               gCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCATTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGcgccc                                                                                                         <  1:621468/140‑1 (MQ=255)

                                  cgcgTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGcgcccgcc                                                                                                      >  2:9155375/1‑140 (MQ=255)

                                  cgcgTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCAACTTTTTCAACGATTAAGcgcccgcc                                                                                                      <  1:1019523/140‑1 (MQ=255)

                                  cgcgTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAAGCCCCCCTCGTTAACCTCCCGTCGGCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCGTTGTCATCGAGTAAGcgcccgcc                                                                                                      >  2:2355222/1‑140 (MQ=255)

                                   gcgTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCt                                                                                                     >  2:6902524/1‑140 (MQ=255)

                                    cgTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCACATCCCCCCTCTTTATCCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTATGCGCCCGCCtg                                                                                                    >  2:3564583/1‑139 (MQ=255)

                                    cgTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCtt                                                                                                    >  1:10021875/1‑140 (MQ=255)

                                    cgTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCtt                                                                                                    >  1:8776329/1‑140 (MQ=255)

                                    cgTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAAAGATTAAGCGCCCGCCtt                                                                                                    <  1:7559608/140‑1 (MQ=255)

                                    cgTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATACCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCtt                                                                                                    >  2:7274282/1‑140 (MQ=255)

                                       tgttgttTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTaa                                                                                                 <  1:3784398/139‑1 (MQ=255)

                                       ggTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTaa                                                                                                 <  2:4917168/140‑1 (MQ=255)

                                       ggTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTaa                                                                                                 <  1:9109991/140‑1 (MQ=255)

                                       ggTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTaa                                                                                                 <  1:731100/140‑1 (MQ=255)

                                          tgttTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCg                                                                                              <  2:10203039/140‑1 (MQ=255)

                                              ttAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTGTTTCAACGTTTAAGCGCCCGCCTTTAATCGTGGt                                                                                          >  2:7578818/1‑140 (MQ=255)

                                               tAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTa                                                                                         >  2:1475766/1‑140 (MQ=255)

                                                  tAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCa                                                                                      >  1:5327254/1‑140 (MQ=255)

                                                    gCACCGCCTGCTTTCGGATCTACCGCAAATGCAAATCCCCCCTCTGTATCCTCCAGTAGTCCTCCCTTTTCGATAGAGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCAtt                                                                                    >  2:827678/1‑140 (MQ=255)

                                                        cGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCt                                                                                <  2:185554/140‑1 (MQ=255)

                                                         gCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTg                                                                               <  1:4934688/140‑1 (MQ=255)

                                                         gCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTg                                                                               <  2:6081308/140‑1 (MQ=255)

                                                         gCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTg                                                                               <  2:2092254/140‑1 (MQ=255)

                                                         gCCTGCTTACTGATCTCCCGCAAATGCAAATCCCCCCTCTTAAACCACCAGAAGTCCTCCCTTTTCGTTAGCGACAATGACGG‑CCCACCCCTCTTTTTCAACGATTAAGCGCCCGCCTATAATCGTGGTATCATTCGCTg                                                                               <  2:741496/140‑1 (MQ=255)

                                                          ccTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTgc                                                                              >  1:4292496/1‑140 (MQ=255)

                                                          ccTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTgc                                                                              >  2:8094825/1‑140 (MQ=255)

                                                          ccTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATAAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTgc                                                                              >  1:2271743/1‑140 (MQ=255)

                                                            tGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTgcgc                                                                            <  1:7081212/140‑1 (MQ=255)

                                                              cTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCa                                                                          <  1:7227427/140‑1 (MQ=255)

                                                              cTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGAGAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCa                                                                          <  1:7704042/140‑1 (MQ=255)

                                                               tttCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCAt                                                                         <  1:1680881/140‑1 (MQ=255)

                                                               tttCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCAt                                                                         <  2:3404315/140‑1 (MQ=255)

                                                                ttCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATa                                                                        <  2:2682999/140‑1 (MQ=255)

                                                                ttCTGATCTACCGCAAATGCAAATCCCCCCTCTATAACCTCCAGTAGTCCTCCCTATACGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATa                                                                        <  2:11435051/140‑1 (MQ=255)

                                                                  cTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTCTCAGTCGCTGCGCCATaaa                                                                      >  2:4744670/1‑140 (MQ=255)

                                                                  cTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCAACTTTTTCAACGATTAAGCGCCCGCATTTATGCTTGGAATCATTCGCTGCGCCaaaac                                                                      >  2:586235/1‑139 (MQ=255)

                                                                   tGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAAc                                                                     <  1:4617925/140‑1 (MQ=255)

                                                                    gATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAAcc                                                                    >  2:6305402/1‑140 (MQ=255)

                                                                      tCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTg                                                                  <  2:390205/140‑1 (MQ=255)

                                                                      tCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTg                                                                  <  1:2848066/140‑1 (MQ=255)

                                                                         aCCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAg                                                               <  1:11425854/140‑1 (MQ=255)

                                                                         aCCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAg                                                               <  1:10889350/140‑1 (MQ=255)

                                                                         aCCGAAAATGCAAATCCCCCCTCTTTAACATCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAg                                                               <  2:6748710/140‑1 (MQ=255)

                                                                           cGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTCTCATTCGCTGCGCCATAAACCTGTAGca                                                             >  1:8075279/1‑140 (MQ=255)

                                                                           cGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                             <  2:561339/140‑1 (MQ=255)

                                                                             caaatgcaaatCCCCCCTCTATCACCTCCACTAGTCCTCCCTTTTCGATAGCGACACAGACGGCCCC‑CCCATCTGAATCACCCATTAAGCGCCCGACTTTAATCGTGGTATCATTCGCAGCGCCATAAACCTGTAGcaca                                                           <  2:10480752/140‑1 (MQ=255)

                                                                              aaatgcaaatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCacac                                                          <  2:2990014/140‑1 (MQ=255)

                                                                                atgcaaatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAATCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCa                                                        <  1:599274/140‑1 (MQ=255)

                                                                                atgcaaatCCCCCCTCTTGAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCa                                                        <  2:5248830/140‑1 (MQ=255)

                                                                                   caaatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCatta                                                     <  1:2242336/140‑1 (MQ=255)

                                                                                    aaatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCattat                                                    >  2:1109854/1‑140 (MQ=255)

                                                                                     aatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCattatt                                                   <  2:5697996/140‑1 (MQ=255)

                                                                                          ccccTCTTTAACCTCCAGTAGTCCTCCCTTTTCGAGAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCg                                              <  2:4189295/140‑1 (MQ=255)

                                                                                            ccTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTa                                            <  2:10306199/140‑1 (MQ=255)

                                                                                            ccTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTa                                            <  1:9996953/140‑1 (MQ=255)

                                                                                                      cTCCAGTAGTCCTCCCATTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTg                                  <  1:10603543/140‑1 (MQ=255)

                                                                                                         cAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTACTCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCcg                               >  2:9487931/1‑140 (MQ=255)

                                                                                                          agtagtCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCcgc                              >  1:5409781/1‑140 (MQ=255)

                                                                                                          agtagtCCTCCCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCcgc                              >  1:3392382/1‑140 (MQ=255)

                                                                                                           ctagtCCTCCCTTTTCGCTAGCGACACAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCcgcg                             <  2:3745779/139‑1 (MQ=255)

                                                                                                              gtCCTCCCATTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCa                          <  1:5777371/140‑1 (MQ=255)

                                                                                                               tcctccCTGTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATCAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCAc                         >  2:8926761/1‑140 (MQ=255)

                                                                                                                cctccCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCAcc                        <  1:2644900/140‑1 (MQ=255)

                                                                                                                 ctccCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTCTCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCAccc                       >  1:10659090/1‑140 (MQ=255)

                                                                                                                 ctccCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCAccc                       >  1:11223856/1‑140 (MQ=255)

                                                                                                                 ctccCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCAccc                       >  1:3333708/1‑140 (MQ=255)

                                                                                                                 ctccCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCAccc                       >  1:9155122/1‑140 (MQ=255)

                                                                                                                 ctccCTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGAAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCAccc                       >  1:10423702/1‑140 (MQ=255)

                                                                                                                    ccTTTTCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCAtt                    >  1:6606234/1‑140 (MQ=255)

                                                                                                                      ttttCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTg                  <  1:3583051/140‑1 (MQ=255)

                                                                                                                        ttCGATAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGaa                >  2:4303566/1‑140 (MQ=255)

                                                                                                                            aTAGCGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAAt            <  2:7004301/140‑1 (MQ=255)

                                                                                                                                cGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAATCCTGTATCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACCATTTGa        >  1:6904554/1‑140 (MQ=255)

                                                                                                                                cGACAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGGTTGCCGCGCCTCCCATTGGAACAATTTGa        >  2:7961201/1‑140 (MQ=255)

                                                                                                                                  aCAAAGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCAGTTGAACAATTTGATa      >  1:4000112/1‑140 (MQ=255)

                                                                                                                                    aaaGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAAc    <  1:8347099/140‑1 (MQ=255)

                                                                                                                                      aGACGGCCCC‑CCCCTCTTTGACAACGAATAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTCAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATGTGATAACCg  <  2:8622815/140‑1 (MQ=255)

                                                                                                                                      aGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg  <  1:3068806/140‑1 (MQ=255)

                                                                                                                                      aGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg  <  1:4463863/140‑1 (MQ=255)

                                                                                                                                      aGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg  <  1:5126170/140‑1 (MQ=255)

                                                                                                                                      aGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg  <  2:5386135/140‑1 (MQ=255)

                                                                                                                                      aGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg  <  2:4513562/140‑1 (MQ=255)

                                                                                                                                      aGACGGCCCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCAATTGAACAATTTGATAACCg  <  1:6264333/140‑1 (MQ=255)

                                                                                                                                      aGACGGCCCC‑CCCATCTTATTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTATCCCACCAGTCTTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGAGAACCg  <  2:10804218/140‑1 (MQ=255)

ACCGAGACGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑CCC‑CCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCG  >  NZ_CP025268/2292451‑2292723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`