BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,858,752	+T	pseudogene (395/2078 nt)	flhA →	formate hydrogenlyase transcriptional activator FlhA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,858,748	1	.	T	98.1%	1945.5 / 21.6	529	pseudogene (391/2078 nt)	flhA	formate hydrogenlyase transcriptional activator FlhA
Reads supporting (aligned to +/- strand): ref base . (5/5); new base T (269/247); total (277/252)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 593 total aligned reads displayed.

ACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑A‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGCGTCGTTAACAATGTCG  >  NZ_CP025268/2858609‑2858882

aCGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCa‑t‑a                                                                                                                                         <  1:8565795/140‑1 (MQ=255)

    agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGACGGGCA‑T‑A‑tttt                                                                                                                                    >  2:6930695/1‑140 (MQ=255)

    agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑A‑tttt                                                                                                                                    >  1:4168398/1‑140 (MQ=255)

    agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑A‑tttt                                                                                                                                    >  2:1909604/1‑140 (MQ=255)

    agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑A‑tttt                                                                                                                                    >  1:3136359/1‑140 (MQ=255)

    agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑A‑tttt                                                                                                                                    >  1:1586096/1‑140 (MQ=255)

    agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑A‑tttt                                                                                                                                    >  1:7804460/1‑140 (MQ=255)

    agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑A‑tttt                                                                                                                                    >  1:844192/1‑140 (MQ=255)

    agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATGTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑A‑tttt                                                                                                                                    >  1:8938931/1‑140 (MQ=255)

    agcaTTTTGTCGAGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑A‑tttt                                                                                                                                    >  1:608494/1‑140 (MQ=255)

       aTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑A‑tttttgg                                                                                                                                 <  1:10966649/140‑4 (MQ=255)

       aTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑A‑tttttgg                                                                                                                                 <  1:2457355/140‑4 (MQ=255)

         tttGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑A‑tttttggtg                                                                                                                               >  2:3926893/1‑135 (MQ=255)

               gcgcCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCtgtg                                                                                                                          <  2:2165771/140‑1 (MQ=255)

                cgcCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGa                                                                                                                         >  2:4139064/1‑140 (MQ=255)

                  cccTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAAt                                                                                                                       >  1:6401112/1‑140 (MQ=255)

                             tGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTAt                                                                                                            >  2:3676857/1‑140 (MQ=255)

                             tGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTAt                                                                                                            >  2:5580828/1‑140 (MQ=255)

                             tGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTAt                                                                                                            >  1:3657936/1‑140 (MQ=255)

                             tGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTAt                                                                                                            <  2:2887997/140‑1 (MQ=255)

                             tGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTAt                                                                                                            >  2:1678451/1‑140 (MQ=255)

                             tGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTAt                                                                                                            >  1:4363169/1‑140 (MQ=255)

                              gCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATg                                                                                                           >  1:10035241/1‑140 (MQ=255)

                               cATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGa                                                                                                          >  2:4633816/1‑140 (MQ=255)

                                aTTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGAc                                                                                                         >  1:11136869/1‑140 (MQ=255)

                                 ttGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACg                                                                                                        >  2:971650/1‑140 (MQ=255)

                                 ttGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACg                                                                                                        >  1:9695858/1‑140 (MQ=255)

                                 ttGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACg                                                                                                        >  1:1319291/1‑140 (MQ=255)

                                 ttGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACg                                                                                                        <  2:1425087/140‑1 (MQ=255)

                                 ttGCAGTTACGAAGAATATTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACg                                                                                                        <  1:7306194/140‑1 (MQ=255)

                                   gCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGAt                                                                                                      <  2:8024835/140‑1 (MQ=255)

                                   gCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGAt                                                                                                      <  2:6217681/140‑1 (MQ=255)

                                   gCAGTTACGAAGAATTTGGTGAACCCTGGCCGCAGCTGGACGCAGGTGGGCTAGTCCCAAAATTTGGTCTCTATTGGCTGATGCCTCTGGCGTCTGAAGGGCATT‑T‑TTTTTGTGGCTGTGTATTTATTCGTTATTTCGAt                                                                                                      <  2:9154480/140‑1 (MQ=255)

                                    cAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATc                                                                                                     >  1:3961014/1‑140 (MQ=255)

                                       ttACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATGTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGGTATGACGATCGcc                                                                                                  >  1:10476001/1‑140 (MQ=255)

                                         aCGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGACGGGCA‑T‑AGTTTTGGTGGCTGTGAATTTATGCGTTATGACGATCGcccc                                                                                                >  1:9372263/1‑140 (MQ=255)

                                         aCGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGcccc                                                                                                >  1:5949140/1‑140 (MQ=255)

                                         aCGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGcccc                                                                                                >  1:5149776/1‑140 (MQ=255)

                                           gaagaaTTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTg                                                                                              <  2:10942951/140‑1 (MQ=255)

                                                 ttttGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGa                                                                                        >  1:808295/1‑140 (MQ=255)

                                                 ttttGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑A‑tttttgg                                                                                                                                 >  2:7531385/1‑95 (MQ=255)

                                                    tgtgAAACCCGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGaaaa                                                                                     <  1:3525975/140‑1 (MQ=255)

                                                     gtgAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGaaaaa                                                                                    <  2:8259698/140‑1 (MQ=255)

                                                           ccTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTc                                                                              >  2:8070501/1‑140 (MQ=255)

                                                               gCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATc                                                                          >  1:8801816/1‑140 (MQ=255)

                                                               gCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATc                                                                          >  1:7414719/1‑140 (MQ=255)

                                                               gCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATc                                                                          >  1:9898410/1‑140 (MQ=255)

                                                                     gCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGc                                                                    >  2:1544110/1‑140 (MQ=255)

                                                                      caggACGCAGGTGGGCTATACCCAAAATTTTGTCACTATTGCCTGATGCCACGGGCGGCGGAAGTGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGAACGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTTCa                                                                   <  2:7401038/138‑1 (MQ=255)

                                                                      cTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCa                                                                   <  2:7750680/140‑1 (MQ=255)

                                                                      cTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCa                                                                   <  2:2090700/140‑1 (MQ=255)

                                                                      cTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCa                                                                   <  1:2133516/140‑1 (MQ=255)

                                                                           cGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACAt                                                              >  1:1811270/1‑140 (MQ=255)

                                                                           cGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACAt                                                              <  2:5202865/140‑1 (MQ=255)

                                                                                gTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACg                                                         <  1:11466090/140‑1 (MQ=255)

                                                                                gTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACg                                                         >  2:10552032/1‑140 (MQ=255)

                                                                                gTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGACTGCAAACATTTACg                                                         <  1:3362052/140‑1 (MQ=255)

                                                                                  gggCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCa                                                       <  2:11082432/140‑1 (MQ=255)

                                                                                     cTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGAt                                                    <  1:9244333/140‑1 (MQ=255)

                                                                                       acaCCCAACATTTGGGCTCTCTAGCCAGATGCCACTGGCGGCGGAAGGGCA‑TAA‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATGTACGCAGATCg                                                  <  2:10819635/138‑1 (MQ=255)

                                                                                        taCCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGt                                                 >  2:10225726/1‑140 (MQ=255)

                                                                                        taCCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGt                                                 >  1:6899012/1‑140 (MQ=255)

                                                                                          cccAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑TCT‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAACAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGttt                                               >  2:8703428/1‑140 (MQ=255)

                                                                                          cccAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGttt                                               >  1:9555070/1‑140 (MQ=255)

                                                                                          cccAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGttt                                               >  2:3349888/1‑140 (MQ=255)

                                                                                             aaaaTTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCCGCAAACATTTACGCAGATCGTTTCTg                                            >  1:10276978/1‑140 (MQ=255)

                                                                                              aactttGGTCACTATTGCCTGAGGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTgt                                           <  1:961610/137‑1 (MQ=255)

                                                                                              aaaTTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTgt                                           <  1:9431809/140‑1 (MQ=255)

                                                                                               aaTTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTgtc                                          >  1:8233867/1‑140 (MQ=255)

                                                                                                    ggTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTTTTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCAc                                     <  1:9067256/140‑1 (MQ=255)

                                                                                                    ggTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCAc                                     <  1:3025511/140‑1 (MQ=255)

                                                                                                     gTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCAcc                                    >  2:11348253/1‑140 (MQ=255)

                                                                                                         cTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAAc                                <  2:6720330/140‑1 (MQ=255)

                                                                                                         cTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAAc                                <  2:3156119/140‑1 (MQ=255)

                                                                                                         cTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAAc                                <  2:359421/140‑1 (MQ=255)

                                                                                                           aTTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACaa                              >  2:4287839/1‑140 (MQ=255)

                                                                                                               ccTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATcc                          >  1:3802792/1‑140 (MQ=255)

                                                                                                                cTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCa                         >  1:2685990/1‑140 (MQ=255)

                                                                                                                cTGATGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCa                         >  2:5375311/1‑140 (MQ=255)

                                                                                                                    tGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGc                     >  2:378309/1‑140 (MQ=255)

                                                                                                                    tGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGc                     >  1:7700267/1‑140 (MQ=255)

                                                                                                                    tGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGc                     >  2:2434156/1‑140 (MQ=255)

                                                                                                                    tGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGc                     >  1:6509189/1‑140 (MQ=255)

                                                                                                                    tGCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGc                     >  1:5632259/1‑140 (MQ=255)

                                                                                                                     gCCACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGAGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGcc                    <  1:1967944/140‑1 (MQ=255)

                                                                                                                      ccACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAATCATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCcg                   >  1:10694290/1‑140 (MQ=255)

                                                                                                                       cACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCcgc                  <  2:5949139/140‑1 (MQ=255)

                                                                                                                       cACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCcgc                  <  1:7077265/140‑1 (MQ=255)

                                                                                                                       cACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCcgc                  <  2:4915411/140‑1 (MQ=255)

                                                                                                                       cACTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCcgc                  <  2:4659291/140‑1 (MQ=255)

                                                                                                                        aCTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCcgcg                 >  1:5426205/1‑140 (MQ=255)

                                                                                                                         cTGGCGGCGGACGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAGAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCCCCGTACAAATCCAGAGCcgcgg                >  2:2763756/1‑139 (MQ=255)

                                                                                                                         cTGGCGGCGGAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGcgt                >  2:11246908/1‑140 (MQ=255)

                                                                                                                              ggcggAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGCGTCGTTa           <  2:7138446/140‑1 (MQ=255)

                                                                                                                               gcggAAGGGAA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCACGCGTCTGCATAGATTTGCGCAGATGGTTTCGGTCGTCACCGAACTGATCGAGTGCCGagtggtgag          >  1:6195568/1‑137 (MQ=255)

                                                                                                                                cggAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGCGTCGTTAAc         >  2:6468663/1‑140 (MQ=255)

                                                                                                                                  gAAGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGCGTCGTTAACaa       >  2:9326260/1‑140 (MQ=255)

                                                                                                                                   aaGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGCGTCGTTAACAAt      >  2:8491312/1‑140 (MQ=255)

                                                                                                                                    aGGGCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGCGTCGTTAACAATg     <  1:4575684/140‑1 (MQ=255)

                                                                                                                                      ggCA‑T‑ATTTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGCGTCGTTAACAATGTc   >  1:4904912/1‑140 (MQ=255)

ACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCA‑T‑A‑TTTTGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGCGTCGTTAACAATGTCG  >  NZ_CP025268/2858609‑2858882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`