Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 3,239,247 (T)6→7 intergenic (+264/‑18) ygjR → / → alx Gfo/Idh/MocA family oxidoreductase/TerC family membrane protein Alx

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252683,239,2411.T95.7% 1927.7 / 68.3 569intergenic (+258/‑24)ygjR/alxGfo/Idh/MocA family oxidoreductase/TerC family membrane protein Alx
Reads supporting (aligned to +/- strand):  ref base . (16/8);  new base T (206/332);  total (222/347)
Fisher's exact test for biased strand distribution p-value = 9.00e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01

Only 100 of 649 total aligned reads displayed.

TGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑G‑T‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGCACATGCCATGACC  >  NZ_CP025268/3239102‑3239375
                                                                                                                                             |                                                                                                                                           
 gtgtgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑G‑t                                                                                                                                            >  1:3128086/1‑140 (MQ=255)
  tgtgACAATACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTGTGTTGGTAGTGAGACCTTGCCGGAAGGCGAGGTCGCTGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTGGGCC‑G‑t‑t                                                                                                                                          >  2:10668357/1‑140 (MQ=255)
  tgtgAAAAAACACCTCCGGTCACCGGGCACCCCGAAAGGAATACGCAGACGTATTCCTTTGGTGGGGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTAGGCATCAATAGCAGCGGCTGACGTCTTCCGACGGTGGCC‑G‑t‑t                                                                                                                                          >  2:7686231/1‑140 (MQ=255)
  tgtgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCTGCGGCTGACGTCTTCCGACGTTGGCC‑G‑t‑t                                                                                                                                          >  2:1151749/1‑140 (MQ=255)
  tgtgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑G‑t‑t                                                                                                                                          <  2:4309351/140‑1 (MQ=255)
  tgtgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑G‑t‑t                                                                                                                                          >  1:2974211/1‑140 (MQ=255)
  tgtgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑G‑t‑t                                                                                                                                          >  2:7052132/1‑140 (MQ=255)
  tgtgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑G‑t‑t                                                                                                                                          >  1:2117367/1‑140 (MQ=255)
  tgtgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑G‑t‑t                                                                                                                                          >  1:9239582/1‑140 (MQ=255)
   gtgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑G‑t‑t‑t                                                                                                                                        >  2:4656955/1‑140 (MQ=255)
    tgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑G‑t‑t‑t‑t                                                                                                                                      >  1:2523797/1‑140 (MQ=255)
       aaaaaCACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑G‑t‑t‑t‑t‑t‑tt                                                                                                                                 <  1:1528895/140‑2 (MQ=255)
              caTCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTa                                                                                                                           <  2:2236080/140‑1 (MQ=255)
              caTCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTa                                                                                                                           >  1:11358293/1‑140 (MQ=255)
              caTCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTa                                                                                                                           >  1:7120763/1‑140 (MQ=255)
                   ggTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGaa                                                                                                                      <  1:6402824/140‑1 (MQ=255)
                     tCACCGGGCAACCCGTAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAATAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACt                                                                                                                    <  2:11100264/140‑1 (MQ=255)
                        ccGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCt                                                                                                                 <  1:4327285/140‑1 (MQ=255)
                           ggCCACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATg                                                                                                              >  2:2703293/1‑140 (MQ=255)
                             cAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGaa                                                                                                            >  1:1027185/1‑140 (MQ=255)
                               aCCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATa                                                                                                          >  1:8419353/1‑140 (MQ=255)
                                   gAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGt                                                                                                      >  1:6572822/1‑140 (MQ=255)
                                        gAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCa                                                                                                 <  2:1312497/140‑1 (MQ=255)
                                        gAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCa                                                                                                 <  1:552752/140‑1 (MQ=255)
                                        gAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTACCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCa                                                                                                 <  2:7264815/140‑1 (MQ=255)
                                         aaTACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCAc                                                                                                <  2:154149/140‑1 (MQ=255)
                                           tACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGc                                                                                              >  2:5088973/1‑140 (MQ=255)
                                             cGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCg                                                                                            <  2:304694/140‑1 (MQ=255)
                                             cGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCg                                                                                            <  2:8624135/140‑1 (MQ=255)
                                             cGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCg                                                                                            <  1:6838536/140‑1 (MQ=255)
                                                aGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTg                                                                                         <  1:5742947/140‑1 (MQ=255)
                                                  aCGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGG‑T‑T‑T‑G‑T‑TATGTGTAAGGACCTTCTATGACTACTGTCGGCACGCCGGTGCt                                                                                       >  1:9344027/1‑140 (MQ=255)
                                                       ttCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATggg                                                                                  >  2:784282/1‑140 (MQ=255)
                                                            tttttGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGa                                                                             >  2:10964337/1‑140 (MQ=255)
                                                              tttGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGAtt                                                                           >  1:9607429/1‑140 (MQ=255)
                                                              tttGTTGTAAGTGAGAACTTGCCGTAAGGAGAGGTCTATGCAAAAAAAGCAGAGGCTGAAGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTTTAAGGAACTTCAATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGAtt                                                                           >  1:608697/1‑140 (MQ=255)
                                                               ttgttgTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTc                                                                          >  1:4021728/1‑140 (MQ=255)
                                                                  ttgtAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCt                                                                       <  1:2288533/140‑1 (MQ=255)
                                                                          gagaCCTTGCCGGAAGGCGAGTTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑G‑T‑T‑T‑TAT‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCtgttgttgt                                                               <  2:10437858/140‑1 (MQ=255)
                                                                          gagaCCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCtgttgttgt                                                               <  1:7780840/140‑1 (MQ=255)
                                                                          gagaCCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCtgttgttgt                                                               <  1:8960189/140‑1 (MQ=255)
                                                                            gaCCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCg                                                             <  1:5393059/140‑1 (MQ=255)
                                                                               cTTGCCGGACGGCGAGGTCGATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCa                                                          <  1:7367393/140‑1 (MQ=255)
                                                                               cTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCa                                                          >  2:2109736/1‑140 (MQ=255)
                                                                                 tGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCAtt                                                        >  1:10841074/1‑140 (MQ=255)
                                                                                  gCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTa                                                       >  2:3795577/1‑140 (MQ=255)
                                                                                       aaGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTg                                                  >  1:111278/1‑140 (MQ=255)
                                                                                        aGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTgg                                                 <  2:3398061/140‑1 (MQ=255)
                                                                                           cGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑G‑TAT‑T‑A‑T‑TATGTGTAAGGAACATCTATGAATAATGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCAATATGCTGGCTa                                              >  1:9089087/1‑140 (MQ=255)
                                                                                               gTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGa                                          <  1:8852599/140‑1 (MQ=255)
                                                                                                tCTATGCATAAAATGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGAc                                         <  2:7124041/140‑1 (MQ=255)
                                                                                                tCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGAc                                         <  1:7532092/140‑1 (MQ=255)
                                                                                                tCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGAc                                         <  2:277790/140‑1 (MQ=255)
                                                                                                tCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGAc                                         <  1:10049006/140‑1 (MQ=255)
                                                                                                tCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGAc                                         <  2:8862598/140‑1 (MQ=255)
                                                                                                 cTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGAcc                                        <  1:950738/140‑1 (MQ=255)
                                                                                                    tGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCtgt                                     >  2:4962763/1‑140 (MQ=255)
                                                                                                    tGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCtgt                                     <  1:6190618/140‑1 (MQ=255)
                                                                                                         aaaaaGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGttgttg                                >  1:1789275/1‑140 (MQ=255)
                                                                                                         aaaaaGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TAGGTGTAAGGAACTTCTATGACTACTGTCGGCACGCCGTTGCTATGGGGCGGATACGCTGTTGTTGTCGCCCTTAAGCTGGCTATCGACCTGttgttg                                >  2:9799988/1‑140 (MQ=255)
                                                                                                          aaaaGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGGTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGttgttga                               >  1:9922386/1‑139 (MQ=255)
                                                                                                          aaaaGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGc                               >  1:338240/1‑140 (MQ=255)
                                                                                                          aaaaGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGc                               >  1:4900435/1‑140 (MQ=255)
                                                                                                          aaaaGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGc                               >  1:2684083/1‑140 (MQ=255)
                                                                                                          aaaaGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGc                               >  1:8110500/1‑140 (MQ=255)
                                                                                                           aaaGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCa                              <  1:1226529/140‑1 (MQ=255)
                                                                                                            aaGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAg                             <  2:8373863/140‑1 (MQ=255)
                                                                                                            aaGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGGTGTTGTCGCCATTCTGCTGGCTATCGACCTGTTGTTGCAg                             >  2:6169009/1‑140 (MQ=255)
                                                                                                            aaGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGGGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGGTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAg                             >  1:3509826/1‑140 (MQ=255)
                                                                                                              gcagcGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAggg                           <  2:3185638/140‑1 (MQ=255)
                                                                                                              gcagcGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAggg                           >  1:8668566/1‑140 (MQ=255)
                                                                                                               cagcGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAgggg                          <  1:9707074/140‑1 (MQ=255)
                                                                                                                 gcggcgGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCCTTATGCTGGCTATCGCCCTGTTGTTGCAGGGGcg                        >  1:5184131/1‑140 (MQ=255)
                                                                                                                   ggCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGcgtc                      <  2:6503566/140‑1 (MQ=255)
                                                                                                                     cTGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGcgtcgt                    <  2:9607428/140‑1 (MQ=255)
                                                                                                                      tGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTg                   <  2:8972203/140‑1 (MQ=255)
                                                                                                                      tGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTg                   <  1:1981933/140‑1 (MQ=255)
                                                                                                                      tGACGTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTATAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGACTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTg                   <  2:7515451/140‑1 (MQ=255)
                                                                                                                         cGTCTTCCGACGTTGGCC‑G‑T‑TGT‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGGTGCTATGGGGCGGTTTCGCTGGTGTTGTCGCCCTGATGCTGGCTATCGACCGGGTGGTGCGGGGGCGGCgggggg                >  1:10655795/1‑140 (MQ=255)
                                                                                                                          gTCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGc               <  2:5992249/140‑1 (MQ=255)
                                                                                                                           tcatCCGACGTTGGCC‑G‑T‑T‑TAT‑T‑TATGTGTTAGGAACTTCTATGAATACTGTCGGCACGCCGGTGCGATGGGGCGGATTCGCGGTTGTTGTCGCCATTATGCTGGCTATCGACCTGGTGTTGCAGGGGCGTCGTGGGGca              <  2:6632481/137‑1 (MQ=255)
                                                                                                                           tCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGca              <  2:5578611/140‑1 (MQ=255)
                                                                                                                           tCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGca              <  2:5753123/140‑1 (MQ=255)
                                                                                                                           tCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGca              <  2:806916/140‑1 (MQ=255)
                                                                                                                           tCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGca              <  1:7028249/140‑1 (MQ=255)
                                                                                                                           tCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGca              <  2:10841073/140‑1 (MQ=255)
                                                                                                                           tCTTCCGACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGCTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGca              <  2:3944808/140‑1 (MQ=255)
                                                                                                                           tCTACCGACGTTGGCC‑GAT‑T‑T‑T‑T‑TATGTGTAAGGACCTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGca              <  1:7899441/140‑1 (MQ=255)
                                                                                                                            cTTCCGACGTTGGCC‑G‑T‑T‑T‑T‑TGTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGcac             >  2:3612680/1‑140 (MQ=255)
                                                                                                                             ttCCGACGTTGTCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATCCTGTCGGCACGACGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGACATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGcaca            <  1:2888739/140‑1 (MQ=255)
                                                                                                                                 gACGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGCACATGcc        <  1:422907/140‑1 (MQ=255)
                                                                                                                                  aCGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGCACATGcca       <  1:5880998/140‑1 (MQ=255)
                                                                                                                                  aCGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGCACATGcca       >  2:8745894/1‑140 (MQ=255)
                                                                                                                                   cGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGCACATGccat      <  1:10457254/140‑1 (MQ=255)
                                                                                                                                   cGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGCACATGccat      <  1:373621/140‑1 (MQ=255)
                                                                                                                                   cGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGCACATGccat      <  2:9096493/140‑1 (MQ=255)
                                                                                                                                   cGTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGCACATGccat      <  2:9378585/140‑1 (MQ=255)
                                                                                                                                   agTTGGCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGCACATGccag      <  1:4624868/139‑2 (MQ=255)
                                                                                                                                       ggCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGCACATGccatgacc  >  1:10973754/1‑140 (MQ=255)
                                                                                                                                       ggCC‑GTT‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGCACATGccatgacc  <  1:827933/140‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                           
TGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑G‑T‑T‑T‑T‑T‑TATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGCACATGCCATGACC  >  NZ_CP025268/3239102‑3239375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑