BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	4,449,793	+T	pseudogene (3757/3779 nt)	tamB →	autotransporter assembly complex protein TamB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	4,449,791	1	.	T	98.1%	3326.6 / 40.5	894	pseudogene (3755/3779 nt)	tamB	autotransporter assembly complex protein TamB
Reads supporting (aligned to +/- strand): ref base . (9/8); new base T (522/349); total (536/358)
Fisher's exact test for biased strand distribution p-value = 6.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 924 total aligned reads displayed.

GTCAGCGGCTATGTATTGCCAGGTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑A‑T‑TGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTAT  >  NZ_CP025268/4449652‑4449925

    gCGGCTATGTATTGCCAGGTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑A‑t‑ttg                                                                                                                                    >  2:2470993/1‑138 (MQ=255)

     cGGCTATGTATTGCCAGGTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑A‑t‑ttgc                                                                                                                                   >  1:4201395/1‑137 (MQ=255)

     cGGCTATGTATTGCCAGGTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑A‑t‑ttgc                                                                                                                                   >  2:7839936/1‑137 (MQ=255)

        cTATGTATTGCCAGGTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTACGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACGGG‑ATT‑TGctct                                                                                                                                 >  1:7724476/1‑140 (MQ=255)

          aTGTATTGCCAGGTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTAt                                                                                                                               >  1:7563721/1‑140 (MQ=255)

               ttGCCAGGTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGtt                                                                                                                          >  1:9166464/1‑140 (MQ=255)

               ttGCCAGGTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGtt                                                                                                                          <  1:7167931/140‑1 (MQ=255)

               ttGCCAGGTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGtt                                                                                                                          <  1:677249/140‑1 (MQ=255)

               ttGCCAGGTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGtt                                                                                                                          <  1:436925/140‑1 (MQ=255)

               ttGCCAGGTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGtt                                                                                                                          >  2:7427614/1‑140 (MQ=255)

               ttGCCAGGTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGtt                                                                                                                          <  2:4463861/140‑1 (MQ=255)

                  ccAGGTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGa                                                                                                                       >  1:8917004/1‑140 (MQ=255)

                  ccAGGTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAAACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGa                                                                                                                       >  1:8157240/1‑140 (MQ=255)

                     ggTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGACTGATGCATAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGtt                                                                                                                    >  1:5134686/1‑140 (MQ=255)

                       tCTGCAAGAGAAATACGGCGTGGGTATAATTGACTATATACCACCACTCACGTTACGTTATCGCCGGATGCCGAAGCTATATCTGGATGCCGTGTCTGGTGTATACCAGTCACTGG‑ATT‑TTCTCTATCAGTTCGAGtttt                                                                                                                  <  1:8515464/140‑1 (MQ=255)

                           cAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCa                                                                                                              <  1:2565023/140‑1 (MQ=255)

                             aGTGAAATACGGCGTGGGTATATTTGTCTCTATAGCAACACTCACGTTACGTTATCGCATGATTCCTATGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAAt                                                                                                            <  2:419116/140‑1 (MQ=255)

                             aGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCGCTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAAt                                                                                                            <  1:5528285/140‑1 (MQ=255)

                                gAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCg                                                                                                         <  1:946195/140‑1 (MQ=255)

                                 aaaTACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGa                                                                                                        >  2:9690152/1‑140 (MQ=255)

                                        gCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTg                                                                                                 >  2:9536349/1‑140 (MQ=255)

                                         cGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTCAGCTAGATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑AGT‑TGCTCTATCAGTTCGAGTTTTAGCACTGCGAATAGTTGt                                                                                                >  2:7655844/1‑140 (MQ=255)

                                         cGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGt                                                                                                >  2:8224194/1‑140 (MQ=255)

                                         cGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGt                                                                                                >  2:707254/1‑140 (MQ=255)

                                           tGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCt                                                                                              >  2:3875192/1‑140 (MQ=255)

                                           tGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCt                                                                                              >  1:4955194/1‑140 (MQ=255)

                                             ggTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTAc                                                                                            >  2:582017/1‑140 (MQ=255)

                                               tataTTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACgg                                                                                          <  2:871700/140‑1 (MQ=255)

                                               tataTTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACgg                                                                                          <  1:2138553/140‑1 (MQ=255)

                                                   tttGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGt                                                                                      >  2:1182322/1‑140 (MQ=255)

                                                     tGACTCTATAGCAACACTCACGTTACGTTATCGCCGGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGttt                                                                                    <  1:6941511/140‑1 (MQ=255)

                                                       aCTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTAc                                                                                  >  1:42938/1‑140 (MQ=255)

                                                       aCTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTACGAGTATTAGCAATGCGAATATATGTCAACGGCAGATTAc                                                                                  >  2:10990844/1‑140 (MQ=255)

                                                         tctATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGc                                                                                >  2:1360795/1‑140 (MQ=255)

                                                         tctATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGc                                                                                >  1:11449065/1‑140 (MQ=255)

                                                           tataGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCca                                                                              >  1:7882832/1‑140 (MQ=255)

                                                           tataGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCca                                                                              >  1:9294053/1‑140 (MQ=255)

                                                             taGCAACACTCACGTTACGTTATCGCCTGATGCCTCAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑AGT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCcaca                                                                            >  1:6158420/1‑140 (MQ=255)

                                                                   cacTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTTCGGCAGTTTACGCCACAAACAAg                                                                      >  1:11120201/1‑140 (MQ=255)

                                                                     cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGc                                                                    >  2:3690499/1‑140 (MQ=255)

                                                                     cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGc                                                                    >  1:1891646/1‑140 (MQ=255)

                                                                     cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGc                                                                    >  2:4335088/1‑140 (MQ=255)

                                                                     cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGc                                                                    <  1:2328051/140‑1 (MQ=255)

                                                                     cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGGTGACGCCTCAATCAAGGc                                                                    >  2:6362288/1‑140 (MQ=255)

                                                                     cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAAGATTTGTCTACGGCAGTTTACGCCACAAACAAGGc                                                                    >  1:8333133/1‑140 (MQ=255)

                                                                       cACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                                  <  2:169113/140‑1 (MQ=255)

                                                                       cACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                                  <  1:3095186/140‑1 (MQ=255)

                                                                       cACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                                  <  1:9732185/140‑1 (MQ=255)

                                                                         cgttacgtgaTCGCCTGATGCCTAAGATATAACTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTGTTAGCACTGCGAATATTTGTCTACGGCAGTTTACGCCACCAACAAGGCAACa                                                                >  2:6041255/1‑140 (MQ=255)

                                                                           ttacgttaTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGt                                                              <  2:850207/140‑1 (MQ=255)

                                                                             acgttaTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCa                                                            >  2:4776414/1‑140 (MQ=255)

                                                                             acgtgaTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATGTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCa                                                            <  1:3300699/140‑1 (MQ=255)

                                                                                 taTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTgg                                                        <  2:3129714/140‑1 (MQ=255)

                                                                                  aTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGa                                                       >  1:9888684/1‑140 (MQ=255)

                                                                                  aTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGa                                                       >  1:1353451/1‑140 (MQ=255)

                                                                                   tCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCCCTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGAt                                                      >  2:5064465/1‑140 (MQ=255)

                                                                                   tCGCCTGATGCCGACGCTATAGCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑A‑TGTGCTCGATCAGTTCGCGTTTTAGCAATGCGAATATTGGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGAt                                                      <  2:9002191/140‑1 (MQ=255)

                                                                                    cGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGTCAGTTTACGCCACAAACAAGGCAACAGTCACTGTATg                                                     >  2:6612740/1‑140 (MQ=255)

                                                                                     gCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGa                                                    <  1:10214959/140‑1 (MQ=255)

                                                                                        tGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCa                                                 >  1:2773802/1‑140 (MQ=255)

                                                                                         gATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCaa                                                <  1:3846115/140‑1 (MQ=255)

                                                                                         gATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCaa                                                <  1:5757912/140‑1 (MQ=255)

                                                                                          aTGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCCATGCGAATAGTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGAACAAt                                               >  2:10268601/1‑140 (MQ=255)

                                                                                           tGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                                  >  2:9853687/1‑120 (MQ=255)

                                                                                           tGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATg                                              <  1:10966731/140‑1 (MQ=255)

                                                                                             ccTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGcc                                            <  2:4240615/140‑1 (MQ=255)

                                                                                             ccTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGcc                                            >  2:10582749/1‑140 (MQ=255)

                                                                                                     atatCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAATCAAGGCAACAGTCACTGGATGTCCAATGCCCAGTTACt                                    >  2:9186408/1‑140 (MQ=255)

                                                                                                      tatCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGa                                                                                                        >  1:8652768/1‑71 (MQ=255)

                                                                                                      tatCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTg                                   >  2:8078996/1‑140 (MQ=255)

                                                                                                           ggAAGCCGTGTCTGGTGTAGACCAGGCACTTG‑A‑TGTGCTCTATCAGTTCGAGTTTTAGCAATGCGACTATTTGTATACGTCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGTGCGa                              >  2:10037220/1‑140 (MQ=255)

                                                                                                              aGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGAttt                           <  2:834826/140‑1 (MQ=255)

                                                                                                                ccGTGTCTGGTGTAGACCAGGCCCTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCCATGCCCAGTTACTGGGCGATTTCa                         >  2:2668119/1‑140 (MQ=255)

                                                                                                                 cGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAg                        <  2:4925587/140‑1 (MQ=255)

                                                                                                                   tgtCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTa                      >  2:11259626/1‑140 (MQ=255)

                                                                                                                        ggTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGAt                 <  1:10114390/140‑1 (MQ=255)

                                                                                                                         gagtACACCAGGCACTGGCT‑T‑TGCTCTATCAGTTCGAGTTTTAGCACTGCGACTATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATa                <  1:6315209/138‑1 (MQ=255)

                                                                                                                           gtAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGAGTTCAGGATCGATAAc              >  1:11235880/1‑140 (MQ=255)

                                                                                                                            tAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACt             <  2:2838317/140‑1 (MQ=255)

                                                                                                                              gACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTAc           <  1:10822245/140‑1 (MQ=255)

                                                                                                                              gACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTAc           <  2:3456784/140‑1 (MQ=255)

                                                                                                                              gACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTAc           <  1:6611080/140‑1 (MQ=255)

                                                                                                                               aCCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTAcc          >  1:10009554/1‑140 (MQ=255)

                                                                                                                               aCCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTAcc          >  1:3201671/1‑140 (MQ=255)

                                                                                                                               aCCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTAcc          >  1:4681508/1‑140 (MQ=255)

                                                                                                                                ccAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCa         <  2:3274555/140‑1 (MQ=255)

                                                                                                                                 cAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAg        <  1:5339994/140‑1 (MQ=255)

                                                                                                                                 cAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAg        <  1:1555005/140‑1 (MQ=255)

                                                                                                                                 cAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAg        <  2:5191901/140‑1 (MQ=255)

                                                                                                                                 cAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAg        >  1:7381456/1‑140 (MQ=255)

                                                                                                                                 cAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAg        <  2:1888383/140‑1 (MQ=255)

                                                                                                                                 cAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTGTAGCATTGCGAATATTGGTCTACGGCAGTGTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTCCTGGGCGATTTCAGTATCGATAACTAcccg        <  2:3030436/140‑3 (MQ=255)

                                                                                                                                   ggCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGNGCGATTTCAGTATCGATAACTACCAGtt      >  1:6011782/1‑140 (MQ=255)

                                                                                                                                   ggCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGtt      >  1:1054295/1‑140 (MQ=255)

                                                                                                                                   ggCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGtt      >  2:2154333/1‑140 (MQ=255)

                                                                                                                                   ggCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTAACTGGGcattg                            <  2:5652093/118‑5 (MQ=255)

                                                                                                                                    gCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTg     >  2:6800220/1‑140 (MQ=255)

                                                                                                                                    gCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTg     >  1:3606843/1‑140 (MQ=255)

                                                                                                                                     cACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGt    <  2:53561/140‑1 (MQ=255)

                                                                                                                                     cACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGt    <  1:10294210/140‑1 (MQ=255)

GTCAGCGGCTATGTATTGCCAGGTCTGCAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑A‑T‑TGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTAT  >  NZ_CP025268/4449652‑4449925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`