BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	400,598	+G	pseudogene (162/260 nt)	iraP →	anti‑adapter protein IraP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	400,594	1	.	G	97.8%	757.8 / 9.0	223	pseudogene (158/260 nt)	iraP	anti‑adapter protein IraP
Reads supporting (aligned to +/- strand): ref base . (4/1); new base G (123/95); total (127/96)
Fisher's exact test for biased strand distribution p-value = 3.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 248 total aligned reads displayed.

GAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑G‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGT  >  NZ_CP025268/400455‑400728

gAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTaga                                                                                                                                           >  2:5616748/1‑140 (MQ=255)

  gttgttATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑g‑g                                                                                                                                       >  2:1090508/1‑140 (MQ=255)

   ttgttATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑g‑g‑g                                                                                                                                     <  2:9377017/140‑1 (MQ=255)

   ttgttATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑g‑g‑g                                                                                                                                     <  1:2508399/140‑1 (MQ=255)

     gttATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑g‑g‑ggg                                                                                                                                   >  1:1592197/1‑139 (MQ=255)

      ttATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑g‑g‑gggc                                                                                                                                  <  2:6417978/140‑3 (MQ=255)

      ttATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑g‑g‑gggc                                                                                                                                  <  2:5246722/140‑3 (MQ=255)

       tATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑g‑g‑gggcg                                                                                                                                 <  1:3624098/140‑4 (MQ=255)

       tATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑g‑g‑gggcg                                                                                                                                 <  1:3998067/140‑4 (MQ=255)

           tAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGt                                                                                                                              >  1:5073174/1‑140 (MQ=255)

           tAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGt                                                                                                                              >  2:10491985/1‑140 (MQ=255)

           tAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGt                                                                                                                              >  2:10591218/1‑140 (MQ=255)

            aaGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTa                                                                                                                             >  1:301802/1‑140 (MQ=255)

             aGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTAc                                                                                                                            <  1:9999376/140‑1 (MQ=255)

             aGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTAc                                                                                                                            >  1:9713198/1‑140 (MQ=255)

             aGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTAc                                                                                                                            <  2:11344609/140‑1 (MQ=255)

             aGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTAc                                                                                                                            >  1:10669426/1‑140 (MQ=255)

             aGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTAc                                                                                                                            >  1:7956982/1‑140 (MQ=255)

              gCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACg                                                                                                                           <  2:1925468/140‑1 (MQ=255)

                 tGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAgg                                                                                                                        >  1:1656005/1‑140 (MQ=255)

                   cccAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCTGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTa                                                                                                                      >  1:4735887/1‑140 (MQ=255)

                   cccAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTa                                                                                                                      >  2:3031039/1‑140 (MQ=255)

                   cccAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTa                                                                                                                      >  1:10575142/1‑140 (MQ=255)

                   cccAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTa                                                                                                                      >  2:1757574/1‑140 (MQ=255)

                    ccAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTaa                                                                                                                     <  1:2264835/140‑1 (MQ=255)

                    ccAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTaa                                                                                                                     <  1:6132081/140‑1 (MQ=255)

                        aaaGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGcc                                                                                                                 <  2:4934322/140‑1 (MQ=255)

                        aaaGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGcc                                                                                                                 <  2:7222397/140‑1 (MQ=255)

                           gaagaagaGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGa                                                                                                              <  1:6837000/140‑1 (MQ=255)

                                   gTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCa                                                                                                      <  1:2905617/140‑1 (MQ=255)

                                     cGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCAtt                                                                                                    >  2:4324012/1‑140 (MQ=255)

                                     cGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCAtt                                                                                                    >  1:4761885/1‑140 (MQ=255)

                                      gAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTc                                                                                                   >  2:1358734/1‑140 (MQ=255)

                                      gAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTc                                                                                                   >  2:4361428/1‑140 (MQ=255)

                                       aaaGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTcc                                                                                                  >  1:2996787/1‑140 (MQ=255)

                                            aCTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTgacg                                                                                             >  1:8314672/1‑140 (MQ=255)

                                              tgtgtgCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTgacgac                                                                                           >  1:11378455/1‑140 (MQ=255)

                                              tgtgtgCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTgacgac                                                                                           >  1:7330542/1‑140 (MQ=255)

                                               gtgtgCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGacgacg                                                                                          <  1:9185109/140‑1 (MQ=255)

                                                tgtgCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCTGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGAcgacga                                                                                         <  2:4988895/140‑1 (MQ=255)

                                                tgtgCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACg                                                                                                                           >  1:4447479/1‑106 (MQ=255)

                                                tgtgCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGAcgacga                                                                                         <  2:9695658/140‑1 (MQ=255)

                                                 gtgCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGAt                                                                                        >  2:826842/1‑140 (MQ=255)

                                                  tgCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATa                                                                                       <  1:11150216/140‑1 (MQ=255)

                                                  tgCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATa                                                                                       >  2:11434526/1‑140 (MQ=255)

                                                  tgCGCAGGTAGAAGCTTTAGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATa                                                                                       >  1:7670354/1‑140 (MQ=255)

                                                       aGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAg                                                                                  <  1:306960/140‑1 (MQ=255)

                                                        ggTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAgc                                                                                 >  2:5741841/1‑140 (MQ=255)

                                                        ggTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAgc                                                                                 >  1:2468805/1‑140 (MQ=255)

                                                          tAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAgctg                                                                               >  1:6563805/1‑140 (MQ=255)

                                                          tAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAgctg                                                                               >  2:7059462/1‑140 (MQ=255)

                                                                 tttGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCACAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGcgcg                                                                        >  1:3878766/1‑140 (MQ=255)

                                                                   tGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGAt                                                                      >  2:2905039/1‑140 (MQ=255)

                                                                   tGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGAt                                                                      >  2:322313/1‑140 (MQ=255)

                                                                   tGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGAt                                                                      >  2:4491236/1‑140 (MQ=255)

                                                                    ggAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGAtt                                                                     <  2:334906/140‑1 (MQ=255)

                                                                    ggAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGAtt                                                                     <  1:3364106/140‑1 (MQ=255)

                                                                         ttATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTa                                                                <  1:7972866/140‑1 (MQ=255)

                                                                          tATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTaa                                                               >  1:2188693/1‑140 (MQ=255)

                                                                            tCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAaag                                                             <  1:1029994/140‑1 (MQ=255)

                                                                             cGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAaaga                                                            <  1:9413464/140‑1 (MQ=255)

                                                                               tCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAaagaag                                                          >  2:8208047/1‑140 (MQ=255)

                                                                                       aTGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGaa                                                  <  2:5072586/140‑1 (MQ=255)

                                                                                       aTGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGaa                                                  <  2:7670353/140‑1 (MQ=255)

                                                                                       aTGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGaa                                                  <  2:3982015/140‑1 (MQ=255)

                                                                                         gCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGACATCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGc                                                <  1:8532104/140‑1 (MQ=255)

                                                                                          cTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTCAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCa                                               >  2:9442963/1‑140 (MQ=255)

                                                                                          cTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCa                                               >  1:4523913/1‑140 (MQ=255)

                                                                                             cGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATcc                                            >  1:4955925/1‑140 (MQ=255)

                                                                                               cAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGGAAAGAAGTTATTGAAGCATCCTc                                          >  1:10027080/1‑140 (MQ=255)

                                                                                                aaTATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCg                                         <  1:1365214/140‑1 (MQ=255)

                                                                                                  tatGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTc                                       >  1:985003/1‑140 (MQ=255)

                                                                                                    tGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAg                                     >  1:11026600/1‑140 (MQ=255)

                                                                                                    tGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAg                                     >  2:6616306/1‑140 (MQ=255)

                                                                                                    tGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAg                                     >  1:422001/1‑140 (MQ=255)

                                                                                                    tGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAg                                     >  1:376067/1‑140 (MQ=255)

                                                                                                    tGGCGCAAAATGACCAACAGCGGTTGAGTGATCCGGGAGAGG‑G‑GGCGCTGTACGAGGTAATGCCCGATGCCCGCATTCCTGACGACGATACGGAGCGGCTGCGCGATTTCGTAATGAAGTTATTGAAGGTTCCTCGTCAg                                     >  2:346483/1‑140 (MQ=255)

                                                                                                     ggCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGt                                    >  2:4754261/1‑140 (MQ=255)

                                                                                                       cgcAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAaag                                                             >  1:878065/1‑113 (MQ=255)

                                                                                                       cgcAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTaa                                  >  1:11281695/1‑140 (MQ=255)

                                                                                                       cgcAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTaa                                  >  2:11072075/1‑140 (MQ=255)

                                                                                                       cgcAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTaa                                  >  1:1788497/1‑140 (MQ=255)

                                                                                                       cgcAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGATATTGAAGCATCCTCGACAGtca                                  >  2:7792600/1‑138 (MQ=255)

                                                                                                         cAAAATGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTaaaa                                >  2:6179828/1‑140 (MQ=255)

                                                                                                            aaTGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTCAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGACGTTATTGAAGCATCCTCGTCAGTAAAAAGt                             >  2:8830225/1‑140 (MQ=255)

                                                                                                              tGACCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTa                           <  2:8517227/140‑1 (MQ=255)

                                                                                                                aCCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAAt                         <  2:1401907/140‑1 (MQ=255)

                                                                                                                aCCAACAGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAAt                         <  1:8929964/140‑1 (MQ=255)

                                                                                                                      aGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTc                   >  1:3374829/1‑140 (MQ=255)

                                                                                                                      aGCGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTc                   >  1:10802110/1‑140 (MQ=255)

                                                                                                                        cGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCaa                 <  2:9636971/140‑1 (MQ=255)

                                                                                                                        cGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCaa                 <  2:8337265/140‑1 (MQ=255)

                                                                                                                        cGGTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGATAATCTTTTCaa                 >  2:796678/1‑140 (MQ=255)

                                                                                                                          gTTGATTGATCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACa               >  1:4886282/1‑140 (MQ=255)

                                                                                                                          gTTGATTGATCAGGTAGA‑G‑GTGGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACTATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCTTCAGTAAAAAGTTAATCTTTTCAACa               >  2:539382/1‑140 (MQ=255)

                                                                                                                           ttgattgaTCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAg              <  2:8716193/140‑1 (MQ=255)

                                                                                                                            tgattgatCAGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGc             <  2:2315308/140‑1 (MQ=255)

                                                                                                                               ttgatCAGGTAGA‑GTG‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGc             <  2:3665415/137‑1 (MQ=255)

                                                                                                                                     aGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATaaa    <  2:2122322/140‑1 (MQ=255)

                                                                                                                                     aGGTAGAGG‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATaaa    <  2:2861298/140‑1 (MQ=255)

GAGTTGTTATTTAAGCTTGCCCAAAAAGAAGAAGAGTCGAAAGAACTGTGTGCGCAGGTAGAAGCTTTGGAGATTATCGTCACTGCAATGCTTCGCAATATGGCGCAAAATGACCAACAGCGGTTGATTGATCAGGTAGA‑G‑G‑GGCGCTGTACGAGGTAAAGCCCGATGCCAGCATTCCTGACGACGATACGGAGCTGCTGCGCGATTACGTAAAGAAGTTATTGAAGCATCCTCGTCAGTAAAAAGTTAATCTTTTCAACAGCTGTCATAAAGT  >  NZ_CP025268/400455‑400728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`