Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252683,505,5590TA21.4% 662.1 / 56.3 309intergenic (+90/+62)phoB/yhfSDNA‑binding transcriptional regulator FrlR/aminotransferase class I/II‑fold pyridoxal phosphate‑dependent enzyme
Reads supporting (aligned to +/- strand):  ref base T (61/170);  new base A (61/2);  total (137/172)
Fisher's exact test for biased strand distribution p-value = 4.59e-26
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 322 total aligned reads displayed.

TCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGC  >  NZ_CP025268/3505420‑3505698
                                                                                                                                           |                                                                                                                                           
tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCct                                                                                                                                             <  2:2483485/140‑1 (MQ=255)
       cgcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACACCCCCACCCCCTCCCCa                                                                                                                                      >  1:5241746/1‑140 (MQ=39)
       cgcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCGCACCCAAACCCACTCCCCa                                                                                                                                      >  1:741038/1‑140 (MQ=255)
         ccAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTGCCCCCACCCCAACCCACAcccccca                                                                                                                                    >  1:2432823/1‑137 (MQ=38)
           aaTCGCATCACCTTACCTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCACTCCCCAAAgg                                                                                                                                  <  2:3804632/140‑1 (MQ=255)
            aTCGCATCACCTTAACTATTGAGAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGACTTTTACATCCCGTACGGTCCCCCCACCCTAAACCACACCCCCAAcgg                                                                                                                                 >  1:188533/1‑140 (MQ=37)
                cATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGa                                                                                                                             <  1:8789105/140‑1 (MQ=255)
                 aTCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGGGCCCACAACCGAAACCACTCCCCAAAgcggggcg                                                                                                                            >  1:3693/1‑136 (MQ=37)
                     ccatcaCTATTGATAATAAAAGACATTACCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCACTCCCCAAAGGGGCGAGGGGa                                                                                                                        <  1:8367621/135‑1 (MQ=38)
                     ccTTCACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGa                                                                                                                        <  1:3084782/140‑1 (MQ=255)
                     ccTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGa                                                                                                                        <  1:7668198/140‑1 (MQ=255)
                      cTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACCACCAAACCCTCTCCCCAAAGGGGCGAGGGGAc                                                                                                                       >  1:11219312/1‑140 (MQ=255)
                          aCTATTGATAATAAAAGACATTAACCGTCGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTCCGTTCCCCACACCCGAACCCTCTCCCCCAAGGGGCGCGGCGCCCggt                                                                                                                   >  2:7637600/1‑138 (MQ=38)
                          aCTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACACCCCAAACCACAACCCACACGGGGGCGGGGGcccgg                                                                                                                   >  1:3584637/1‑137 (MQ=25)
                          aCTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGtt                                                                                                                   <  1:1787300/140‑1 (MQ=255)
                              ttGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTAACCCAAACCCACTACCCAAAGGGGCGAGGGGAACGTTAAGt                                                                                                               >  2:2962665/1‑140 (MQ=38)
                              ttGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCAAACCCGAACCCGCTCCCCAAAGGGGCGAGGGGACCGGTCAGt                                                                                                               >  1:11387105/1‑140 (MQ=38)
                               tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTa                                                                                                              <  1:10962694/140‑1 (MQ=255)
                               tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCAACCAAAACCTCAACCCAAAGGGGCGAGGGGACCGATCAgaa                                                                                                              >  2:11189817/1‑138 (MQ=38)
                               tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTGCCCCGCACCCTGACCCACTCCCCCAATGGGCGCGGGGACCCTTCAGTa                                                                                                              >  2:7785266/1‑140 (MQ=38)
                                gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCCAACCCAAACCCCCTACCCAACGGGGGGAGGGGGCCGGTGAGtcc                                                                                                             >  1:10633220/1‑138 (MQ=37)
                                gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTAc                                                                                                             <  2:1882905/140‑1 (MQ=255)
                                gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAACCCACTACCCCAAGGGGCGAGGGGACCGTTCAATAc                                                                                                             >  2:3462313/1‑140 (MQ=38)
                                gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAACCCACACCCCAAATGGGCGAGGGGTCCGGTCAGTaa                                                                                                             >  2:411703/1‑139 (MQ=38)
                                gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACAACCGAACCCTCTACCCAAAGGGGCGAGGGGTCCGTTGCGTAc                                                                                                             >  1:6308794/1‑140 (MQ=38)
                                gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACACCCTAACCCACACCCCCAAGGGGCGAGGGGACCGTTCAGTAc                                                                                                             >  1:9948762/1‑140 (MQ=39)
                                     ttaaaGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTTCGTTCCCCGCCCCCTAACCCACTCCCCAATGGGGCGAGGGGACCGGTCCGGACGGAAt                                                                                                        >  2:8346214/3‑140 (MQ=37)
                                     taaAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCCAATGGGCGAGGGGACCGTTCAGTAAGGaaa                                                                                                        >  2:6573327/1‑139 (MQ=255)
                                      aaaaGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAAtt                                                                                                       <  1:4025937/140‑1 (MQ=255)
                                           aCATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCACCCCCAAGGGGCGAGGGGACCGGTCCGTACCGAATTTAGCt                                                                                                  >  2:9064329/1‑140 (MQ=38)
                                             aTTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTg                                                                                                >  2:6664302/1‑140 (MQ=255)
                                             aTTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTGGCCCGCACCCCAACCCGCAAACCCAAAGGGCGCGGGGGCCCGTGACTACGGGAATTAGcatt                                                                                                >  1:8926537/1‑137 (MQ=19)
                                              ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTACCCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                               <  2:365406/140‑1 (MQ=255)
                                              ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                               <  1:4919131/140‑1 (MQ=255)
                                              ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                               <  1:6717843/140‑1 (MQ=255)
                                              ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                               <  2:2899815/140‑1 (MQ=255)
                                              ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGAAATTTAGCTTGa                                                                                               <  1:6948074/140‑1 (MQ=255)
                                              ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCAGACGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCGTGa                                                                                               <  2:8538583/140‑1 (MQ=255)
                                              gtAACCGTAGGCCGGATAAGAGGCGCCAGCATCGCCTCCGGCGATGCTGGCGCGTTGAATTGTACATCCCGAACGTTCCCCTCACCCTACCCCTCTCCCCACCGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                               <  2:9700989/139‑1 (MQ=255)
                                               tAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTCCGTGGCCCACCACCAAACCCACAACACCAAGGGGCGGGGGGACCGGTCCGGACAGTATTTAGCTTgtg                                                                                              >  1:9768849/1‑138 (MQ=19)
                                                 aCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACgg                                                                                            <  2:1412599/140‑1 (MQ=255)
                                                   cGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                          <  2:6447234/140‑1 (MQ=255)
                                                   cGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                          <  1:8668567/140‑1 (MQ=255)
                                                     tAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCACTCCCCAAAGGGGCGAGGGGACCGTTCACTAAGGAATATAGCTTGAAGGTGCt                                                                                        >  2:8521033/1‑140 (MQ=38)
                                                      aGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTGTACATCCCGTACGTTCCCCACACCCTAACCCTCTCCCCCAAGGGGCGTGGGGACCGGTCAGTTCGGAATTGAGCTTGTCGGTGCtg                                                                                       >  2:10166838/1‑139 (MQ=38)
                                                       ggCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACACCCTAACCCAAAACCCAAAGGGGCGAGGGGACCCTTCAGTACCGAAATTATCTTTAAGGTGCTaa                                                                                      >  2:6986339/1‑139 (MQ=37)
                                                       ggCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCCAACCCGCTCCCCACAGGGGCGGGGGGTCCGGGCCGTGCGGCATTTAGGTTGACggggggct                                                                                      >  1:7152818/1‑134 (MQ=25)
                                                        gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGTGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATc                                                                                     <  1:1324645/140‑1 (MQ=255)
                                                          cGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCa                                                                                   <  1:7932477/140‑1 (MQ=255)
                                                          cGGAGAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCATGACGGTGCTATCCa                                                                                   <  1:8084742/140‑1 (MQ=255)
                                                            gATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCCAAACCAAAACCAAAACCCCAAAGGGCGAGGGGACCGTGATGAACCGTATTTATGTTGAAGGTGGAATCCacc                                                                                 >  2:5003967/1‑138 (MQ=255)
                                                             aTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAACGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCa                                                                                <  1:1629622/140‑1 (MQ=255)
                                                             aTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCa                                                                                <  1:2289614/140‑1 (MQ=255)
                                                             aTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACAGCCCGTACGTTCCCCAAACCCAAACCCACAACCCCAAGGGGCGTGGGGCCCGTTCAGTACGGGAATTATCGTGAAGGTGCTATccacca                                                                                >  1:9649259/1‑140 (MQ=255)
                                                               aaGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCAtt                                                                              <  1:847758/140‑1 (MQ=255)
                                                               aaGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCAtt                                                                              <  2:4256856/140‑1 (MQ=255)
                                                                agctGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCACACCCTAACCCTCACCCCCAAGGGGCGAGGGGACCGTTCAGTCCGGAATTTAGCTTGACGGTGCTATCCAGCATTa                                                                             <  1:8587771/137‑1 (MQ=38)
                                                                   tGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCCAACCCACTCCCCAAAGGGGCGAGGGGACCGTTCAGGACGGAAATTTTCTTGACGGTGCTATCCAGCCTTAAAt                                                                          >  2:5239721/1‑140 (MQ=38)
                                                                   agcgcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCACTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGATTGACGGTGCTATCCAGCATTAAAt                                                                          >  2:9557282/2‑140 (MQ=255)
                                                                    gcgcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATa                                                                         <  2:5475097/140‑1 (MQ=255)
                                                                    gcgcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTGTACATCCCGTACGTTCCCCTCACCCTACCCCTCTCCCCAACGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCGTGACGGTGCTATCCAGCATTTAATa                                                                         <  2:10625194/140‑1 (MQ=255)
                                                                     cgcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATAc                                                                        <  1:7000328/140‑1 (MQ=255)
                                                                     cgcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACAGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATAc                                                                        <  2:4603276/140‑1 (MQ=255)
                                                                      gcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACt                                                                       <  2:3173443/140‑1 (MQ=255)
                                                                       ccAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCCCACCCCAACCCACTCCCCCAAAGGGCGGGGGGGCCGGTAAGTAAGGAATTTATCTTGACGGTGCTATCCAGCATTaaaaaatg                                                                      >  2:5637336/1‑135 (MQ=255)
                                                                       ccAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTGCCCACACCCAAACCCACACCCCAAAGGGGCGCGGGGCCCCTTCAGTACGGGATTTAGCTTGTCGGTGCTATCCCGCATTAAATACTg                                                                      >  1:10196917/1‑140 (MQ=255)
                                                                             tcgcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATa                                                                <  1:5637694/140‑1 (MQ=255)
                                                                               gcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATAct                                                              <  1:5391854/140‑1 (MQ=255)
                                                                                         aTGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCaaa                                                    <  1:4354329/140‑1 (MQ=255)
                                                                                           gCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCTAAACCACAACCCAAATGGGCGAGGGGACCGTTAAGTACGGTAATTATCTTTAAGGTGCTATCCAGCATTAAATAAAGGCAAAACACTAACGCAAAAt                                                  >  2:5772933/1‑140 (MQ=255)
                                                                                               gcgcGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCgc                                              <  1:5790420/140‑1 (MQ=255)
                                                                                               gcgcGTTGAATTTTACATCCCGTACGTTCCCCTCACCCGAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCgc                                              <  1:5003968/140‑1 (MQ=255)
                                                                                                  cgTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAACGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCgcagc                                           <  2:4152545/140‑1 (MQ=255)
                                                                                                  cgTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCgcagc                                           <  1:10039890/140‑1 (MQ=255)
                                                                                                    ttGAATTTTACATCCCGTACGTTCCCCTCACCCAAAACCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATACAGCCTTAAATAATGGCAATAATCTCACGCAAAATACGCAGCAc                                         >  2:11222936/1‑140 (MQ=38)
                                                                                                    ttGAATTGTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCAc                                         <  1:10097724/140‑1 (MQ=255)
                                                                                                     tGAATTTTACATCCCGTACGTTCCCCTCACCCTACCCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCAcc                                        <  2:2110416/140‑1 (MQ=255)
                                                                                                     tGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCACAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCAcc                                        <  2:6059237/140‑1 (MQ=255)
                                                                                                       aaTTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGt                                      <  2:1041503/140‑1 (MQ=255)
                                                                                                              cATCCCGTACGTTCCCCTCACCCAAACCCACAACCCAAAGGGGCGAGGGGACCGTTCAGTACGGAAAATAGCTTGACGGTGCTATACAGCAATAAATACTGGCAAAACACTCACGCAAAATCCGCAGCACCGtcaattaa                               >  2:910988/1‑134 (MQ=37)
                                                                                                                  ccGTACGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGc                           <  1:3449863/140‑1 (MQ=255)
                                                                                                                    gTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTg                         <  1:10354868/140‑1 (MQ=255)
                                                                                                                        gTTCCCCTCACCCTAACCCTCTCCCCCAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGAt                     <  1:7482473/140‑1 (MQ=255)
                                                                                                                          tCCCCTCACCCTCACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTc                   <  2:8720454/140‑1 (MQ=255)
                                                                                                                          tCCCCTCACCCGCACCCTCTCCCCACAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTc                   <  1:9005762/140‑1 (MQ=255)
                                                                                                                           ccccGCACCCTAACCCACAACCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAAAACACTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCg                  >  2:10281575/1‑140 (MQ=39)
                                                                                                                             ccTCACCCTATCCCTCTCCACATAGTGGCGAGGGGAACGTTCATTACGGAATTTAGCTTGACGTTTCTATGCAGGATTAAATACTGGGAATTCTCTCACGCAAAATCCGCAGCACAGTCTCTTCACCGCTGATATTAggg                >  2:10872875/1‑140 (MQ=255)
                                                                                                                              cTCACCCTAACCCTCTCCCCACAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGt               <  1:6482394/140‑1 (MQ=255)
                                                                                                                               tCACCCAAAACCACACCCCAAAGGGGCGAGGGGACCCTTCAGTACCGAATTTAGCTTTAAGGTGCTAACCAGCATTAAATAAAGTCAATACACTCACGCAAAATCCGCAGCAACCTATCATCACCGCAGCGAATAGGGtt              >  2:8939254/1‑140 (MQ=255)
                                                                                                                                  cccTAACCCTCTCCCCACAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAAt           <  2:9927594/140‑1 (MQ=255)
                                                                                                                                  cccTAACCCTCTCCCCACAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAAGACTGGCAATACTCTCACGCAAACTCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAAt           <  2:7200928/140‑1 (MQ=255)
                                                                                                                                  cccTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAAt           <  1:4229235/140‑1 (MQ=255)
                                                                                                                                      aaCCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTATTGCGa       >  2:2317224/1‑140 (MQ=255)
                                                                                                                                      aaCCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTATCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATGCGGGTTAATGCGa       >  2:1085202/1‑140 (MQ=255)
                                                                                                                                       aCCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGaa      >  1:3963963/1‑140 (MQ=255)
                                                                                                                                       aCCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGaa      <  2:8256055/140‑1 (MQ=255)
                                                                                                                                       aCCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGaa      >  1:4550895/1‑140 (MQ=255)
                                                                                                                                           tctcCCCAAAGGGGCGAGGGGACCTTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTATTCACCGCTGCGAGTCGGGTTAATGCGAATCGc  >  2:4875378/1‑140 (MQ=255)
                                                                                                                                           tctcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGc  >  1:4093233/1‑140 (MQ=255)
                                                                                                                                           tctcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCGCTTCACCGCTGCGATTCGGGTTAATGCGAATCGc  >  1:2652165/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                           
TCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGC  >  NZ_CP025268/3505420‑3505698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑