Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP025268164,3930AT21.0% 341.4 / 10.7 170intergenic (+28/‑168)hrpB/mrcBATP‑dependent helicase HrpB/bifunctional glycosyl transferase/transpeptidase
Reads supporting (aligned to +/- strand):  ref base A (66/66);  new base T (1/34);  total (67/103)
Fisher's exact test for biased strand distribution p-value = 3.86e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 201 total aligned reads displayed.

AGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTT  >  NZ_CP025268/164254‑164532
                                                                                                                                           |                                                                                                                                           
cggAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTATGGTTGGTTTTCTCCCTCGCCCTGTGGga                                                                                                                                             <  1:1802994/139‑1 (MQ=255)
aGGAGCGTACCGTGCGGTGCAAAAAGATATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACGGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGga                                                                                                                                             <  2:2348103/140‑1 (MQ=255)
aGGAGCGTACCGTGAGGTGCAAAAAGAGATGAACGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGga                                                                                                                                             <  1:4712333/140‑1 (MQ=255)
aGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGga                                                                                                                                             <  1:757645/140‑1 (MQ=255)
aGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGga                                                                                                                                             <  2:7707527/140‑1 (MQ=255)
aGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGga                                                                                                                                             <  1:3936222/140‑1 (MQ=255)
aGGAGCGTACCGTGAGGTGCAAAAAGAGATCAAAGGGCGTAAACCCAACCATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGga                                                                                                                                             <  1:4868575/140‑1 (MQ=255)
aGGAGCGTACCGTGAGGTGAAAAAAGATATGAAAGGGCGTTAACCCAAACATGTCTGGCCGGACGACCCGGAAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGga                                                                                                                                             <  1:8206126/140‑1 (MQ=255)
aGGAGCGTACCCTGAGGTGCAAACCGAGATGAAAGGGCGTGATCCCAACCCTGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGga                                                                                                                                             <  2:11008664/140‑1 (MQ=255)
 ggAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGgag                                                                                                                                            <  2:1872944/140‑1 (MQ=255)
 ggAGCGTACCGTGAGGTGCAAAAAGACATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCTGCAAATACTGCACCGACGCGACTGACGAAAAAGTATTCGTAAGGTTGGTGTTCTCCCTCTCCCTGTGGgag                                                                                                                                            <  1:8754217/140‑1 (MQ=255)
  gAGCGTATCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGgaga                                                                                                                                           >  2:2656184/1‑140 (MQ=255)
  gAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGgaga                                                                                                                                           <  1:7139181/140‑1 (MQ=255)
  gAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGgaga                                                                                                                                           >  1:8866264/1‑140 (MQ=255)
  gAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTAGTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGgaga                                                                                                                                           >  1:4256391/1‑140 (MQ=255)
     cGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTCTCCCAACCATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGGTTTCTCCCACTCCCTGTGGGAGAggg                                                                                                                                        <  2:10260186/140‑1 (MQ=255)
     cGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAATCATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAATTAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGTGCggg                                                                                                                                        >  1:799220/1‑140 (MQ=255)
     cGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAggg                                                                                                                                        <  2:1601618/140‑1 (MQ=255)
     cGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAggg                                                                                                                                        >  2:9004602/1‑140 (MQ=255)
      gTACCGTGCGGTGCAAAAAGAGATTAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGc                                                                                                                                       <  1:5016201/140‑1 (MQ=255)
      gTACCGTGAGGTGCAAACAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGc                                                                                                                                       <  2:3517258/140‑1 (MQ=255)
       tACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGcc                                                                                                                                      <  2:7771220/140‑1 (MQ=255)
        aCCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCg                                                                                                                                     <  2:10436909/140‑1 (MQ=255)
            tGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGt                                                                                                                                 >  2:7112914/1‑140 (MQ=255)
               ggTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAg                                                                                                                              >  2:3017701/1‑140 (MQ=255)
                 tGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAggg                                                                                                                            >  1:4121671/1‑140 (MQ=255)
                 tGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAggg                                                                                                                            >  1:1468458/1‑140 (MQ=255)
                   cAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGcc                                                                                                                          >  2:1325509/1‑139 (MQ=255)
                    aaaaaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAt                                                                                                                         >  2:8335527/1‑140 (MQ=255)
                      aaaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAATGTATTCGTAAGGGTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATcc                                                                                                                       >  1:8555770/1‑139 (MQ=255)
                          agaTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCAGGGGTGAGGGCATCAgcgc                                                                                                                   >  2:7670306/1‑140 (MQ=255)
                           gaTGAAAGGGCGTTATCCCAAACTTGTCTGGCCGTACGACCCGGCAAATACGGCACCGACGCGACGTACTAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAgcgcg                                                                                                                  <  2:9518351/140‑1 (MQ=38)
                           gaTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAGCcgcgcg                                                                                                                  >  1:8412351/1‑140 (MQ=255)
                             tGACAGGGCGTAATCCCAACCATGTCGGGCGGGCCGCCCCGGAAAATACTCCACCGACGCGACGGACGAAAAATTATTCGTATGGTTGGTTTTATCCCACTCCCTGGGGTATTGGGCCGGGGTGAGGGCATCAGCGCGCa                                                                                                                <  2:6996005/140‑1 (MQ=255)
                             tGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCa                                                                                                                >  1:2114301/1‑140 (MQ=255)
                                  gggCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGttg                                                                                                           >  1:5730493/1‑139 (MQ=255)
                                    gCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGtgggg                                                                                                         >  1:5432118/1‑136 (MQ=255)
                                    gCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcac                                                                                                         >  2:11417653/1‑140 (MQ=255)
                                     ggtttccccACACATGTGGGGCCGGACGCCCGGGAAACTAGGGCCCCGCGGCGCCGGACGAAAAAGTATTCGTATGGTGGGTTTTCGCCCTCGCCGTGTGGGTTTGGGCGGGGGTGCGGGCCTCAGCGCGCACGTtcacc                                                                                                        <  2:4250899/134‑1 (MQ=255)
                                       ttATCCCAAACATGTCTGGCCGTAGGACCCGGCAATTACTGCACCGACGCGCCGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGTTGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccct                                                                                                      <  2:10011989/140‑1 (MQ=38)
                                       ttATCCCAAACATGTCTGGCCGGCCGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTACGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccct                                                                                                      <  2:4945745/140‑1 (MQ=255)
                                       ttATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccct                                                                                                      >  1:3045521/1‑140 (MQ=255)
                                       ttATCCCAAACATGTCTGGCCGGACGACCCGGAAAATCCTGCACCGACGCGAGGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccct                                                                                                      <  2:6309915/140‑1 (MQ=255)
                                       ttATCCAAAATTTTTCTGGCCGTACTACCGGTCAAATACTGAACGTCCGGGACGGACTAAAAATTTTTCTTATGGTTGGTTTTATCCCTCTCCCTGTGGGTGTGGGCGGGGGTGAGGGCCTCAGCGCGCACGTtcaccct                                                                                                      <  2:3419064/140‑1 (MQ=255)
                                       ttAACCCAAACAGGTCTGGCGGGACGACCCGGAAAATACTCCCCCGACGCGACGTACGAAAATGTTTGCGTATGGTTGTTTTTCTCCCTCGCCCTGTGGGTGAGGGCCGGGGTGTGGGCCTCAGCGCGCACGTtcaccct                                                                                                      <  2:2249742/140‑1 (MQ=255)
                                       gtagcccAAACATGTCTGGCCGGACGACCCGGCAAATACTCCCCCGACGCTCCTGCCGAAAAAGTATTCGTACGGTTGGTTGTCTCCCTCTCCCTGTGGGTGAGGGCCGGGGGGAGGGCATCAGCGCGCCCGTtcaccct                                                                                                      <  2:6699310/136‑1 (MQ=255)
                                         aTCCCAAACTTGTCGGGCCGGACGACCCGGAAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCGGGGGTGAGGGCATCAGCGCGCACGTtcaccctca                                                                                                    <  2:8072700/140‑1 (MQ=255)
                                              aaaCATGTCTGGCCGCACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTAGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAACCGCGCGCACGTTCACCCGAACCCTa                                                                                               >  2:7173529/1‑140 (MQ=38)
                                               aaCTTGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTATGGTTGGTTTTCGCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTaa                                                                                              <  2:8298143/140‑1 (MQ=255)
                                               aaCATGTCTGGCCGGACGACCCGGCAAATACTGCACCGCCGCGACGGTCGAATATGTCTTGGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGTGGGCGGGGGTGAGGGCAGCCGCGCGCACGGtgaccctcacccgtc                                                                                              >  2:11342610/1‑137 (MQ=25)
                                               aaCATGTCGGGCCGGACGACCCGCCCAATACTCCCCCGCCGCGACGGACGAAAAAGTATTCGAAAGGTTGGATTACTCCCTCCCCCTGTGGGCGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTaa                                                                                              <  2:9413698/140‑1 (MQ=255)
                                                    gTCTGGCCGTACGACCCGGAAAATACGGCACCGACGCGACGGCCGAAAAAGTATTCGTATGTTTGGTTTTCTCCCTCGCCCTGTGGGAGTGGGCCGGGTTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCctc                                                                                         <  1:10589277/140‑1 (MQ=255)
                                                    gTCTGGCCGGACGCCCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGTGAGGGCCGGGGGGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCctc                                                                                         <  2:858233/140‑1 (MQ=255)
                                                    gTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAATTTATTCGTATGTTTGGTTTTCTCCCTCTCCCTGTGGGATAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCctc                                                                                         <  2:6790460/140‑1 (MQ=38)
                                                    gTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAATTATTCGTACGGTTGTTTTTCTCCCTCTCCCTGTGGTAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCctc                                                                                         <  2:11374189/140‑1 (MQ=255)
                                                    gTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCgg                                                                                                                                    <  2:4100243/97‑1 (MQ=255)
                                                    gTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCgg                                                                                                                                    >  1:4100244/1‑97 (MQ=255)
                                                    gTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGGCCCGCACCCAAAaccac                                                                                         >  1:4649752/1‑138 (MQ=37)
                                                    gTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGGAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTGGTCCCGCACCCCAAcccac                                                                                         >  1:9781916/1‑138 (MQ=37)
                                                    gTCTGCCCGGCCGCCCCGCCAAATCCTGCCCCGCCGCGACGGCCCAAAACTTATTCGTACGTTGGGTTTTCGCCCCCCCCCGGTGGGCGCGGGCCGGGGTGAGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCctc                                                                                         <  1:6185093/140‑1 (MQ=255)
                                                    gTCTGCCCGCACGCCCCGCCAAATACTCCACCCACGCGCGGCACTAAAATTTTTTCTTCTGGTTGTTTTACCCCCTCGCCCTGTGGTAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCctc                                                                                         <  1:6357934/140‑1 (MQ=16)
                                                    gTCGGGCGGGACGACCCGGCAAATACGGCACCGACGCGCCGGACGAAAAATTATTCGTATGGTTGGTTTTCTCCCTCTCCCTGGGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCctc                                                                                         <  2:5725071/140‑1 (MQ=37)
                                                             tacgccCCGTAAAATACTCCACCGCGGCGCCGGACGAAAATGTATTGTTACGGTTGGTTTTCGCCCTCCCCCGGGGGGTGAGGCCGGGGGTGAGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAg                                                                                <  2:2298653/139‑1 (MQ=255)
                                                             gacgacCGGGCAAATACGGCCCCGCCGCGCGGGACTAAAATGTTTTCGTTGGGTGGGTTTTCGCCCTCTCCGTGGGGGAGTGGGCGGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAg                                                                                <  2:978458/140‑1 (MQ=255)
                                                             gacgacCCGGCAAATCCTGCACCGACGCGACGGACGACAACGTATTCTTAAGGTGGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGTCACCCTCACCCTAACCCTCTCCCTCAAg                                                                                <  2:10799416/140‑1 (MQ=37)
                                                                 acCCGGCAAATCCTGCACCGCCGCGCCGCACGAAACAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGgag                                                                            <  2:10114964/140‑1 (MQ=38)
                                                                 acCCGGCAAATCCGGCCCCGACGCGCCGGACGAAAACGTATTCGTATGGTTGGTTTACTCCCACACCCTGTGGGAGTGGGCGGGGGGGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGgag                                                                            <  2:2207941/140‑1 (MQ=255)
                                                                 acCCGCCAAATCCGGCCCCGCCGCGACGCACGAAAAAGTTTTCGAACGGTGGGTTTTCGCCCTCACCCTGGGGGAGAGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGgag                                                                            <  2:9195714/140‑1 (MQ=255)
                                                                   ccGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGga                                                                                                                                             <  2:8427907/73‑1 (MQ=255)
                                                                   ccGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCACACCCAAACCCACACCCACAAGGGagag                                                                          >  2:3759418/1‑140 (MQ=37)
                                                                      gCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGTCATCAGCGCGCACGTGCACCCTCACCCAAAACAAATCCCACAAGGGCGAgggg                                                                       >  2:6121702/1‑140 (MQ=37)
                                                                      gCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAGCAGCGCGCACGTTCACCCACACCCAAAACCACACCCACAAGGGGGAgggg                                                                       >  2:2725223/1‑140 (MQ=37)
                                                                           tACTGCACCGACGCGACGGACGAAAAAGTATTCGTACGGTTGTTTTTCTCCCTCACCCTGTGGTAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGa                                                                  <  1:7428609/140‑1 (MQ=39)
                                                                              tGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGACCCGCCCCCAAAACCACTCCCACAATGGAGAGGGGGCCgcaag                                                               >  1:10636769/1‑136 (MQ=25)
                                                                                cACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCCCACCCTAACCCGCAACCTCCACGGAGTGGGGGCCGCTCGAg                                                             >  2:9088067/1‑140 (MQ=25)
                                                                                cACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCGAACCCACTCCCACAAGGGAGAGGGGACCCATCCAg                                                             >  2:6460375/1‑140 (MQ=37)
                                                                                  ccGACGCGACGTACTAAAAAGTATTCGTATGTTTGTTTTTCTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGca                                                           <  2:3531782/140‑1 (MQ=38)
                                                                                          acggacgAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCCCCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAttta                                                   <  2:10350849/140‑2 (MQ=255)
                                                                                            ggacgAAAAATTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga                                                 <  2:3912983/140‑1 (MQ=255)
                                                                                               ctaaaatGTATTCGTATGGTTGGTTTTCTCCCTCTCCCGGGGGTTGAGGGCCGGGTTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgagag                                              <  1:9207458/138‑1 (MQ=38)
                                                                                               cgAAATAGTATTCGTACGGTGGGTTTGCGCCCTCTCCCTGGGGGCGCGGGCCGGGGTTCGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgagag                                              <  1:6510324/140‑1 (MQ=37)
                                                                                               cgAAAATGTATTCGTATGGTTGGTTTTCTCCCTCTCCCTGTGGGTGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgagag                                              <  2:1468458/140‑1 (MQ=255)
                                                                                                    atgTATTCGTAAGCTTGGTCTTCTCCCTCTCCCTGTGGTAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGTCACCCTCACCCTAACCCTCTCCCTCAAGGGGGAGGGGACCGATCGAGCACAAAAGTTGAGAGATAtc                                         <  2:1211216/138‑1 (MQ=37)
                                                                                                     aGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCGCAAGGGAGAGGGGACCGATCGAGCACCAATTTTGTGAGATAtct                                        >  2:1806482/1‑140 (MQ=255)
                                                                                                       tctccGTACGGTGGGTGTCCTCCCTCCCCCGGTGGGAGTGGGCGGGGGTGAGGCCCTCAGCGCGCACGTGCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttc                                      <  1:10029109/136‑1 (MQ=37)
                                                                                                       tATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCAACCTCACCCAAAACCACTCCCACAAGGGAGAGGGGGCCCCGCGAGCCCAACATTTTATAGATGtcttc                                      >  1:9524247/1‑140 (MQ=21)
                                                                                                          tcttAAGGTTGGTTTTCACCCTCTCCCTGTGGGTGCGGGCCGGGTTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttcttc                                   <  1:11417654/137‑1 (MQ=39)
                                                                                                          tCGTAAGGTTGGTTTTCTCTCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttcttc                                   <  1:8538703/140‑1 (MQ=255)
                                                                                                          tCGTAAGGTTGGTTTTCTCCCTCACCCTGTGGGAGAGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttcttc                                   <  1:10253509/140‑1 (MQ=255)
                                                                                                            gTATGGTTGTTTTTCACCCACTCCCTGTGGTAGTGGGCGGGGGTGAGGGACTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTg                                 <  1:4720753/140‑1 (MQ=38)
                                                                                                             aacggtgggttTTCGCCCCCCCCGGGTGGGAGAGGGCCGGGGTGCGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGt                                <  1:3486193/137‑1 (MQ=38)
                                                                                                               tggttggttTTCCCCCACTCCCTGTGGGTGTGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCt                              <  1:5865365/139‑1 (MQ=39)
                                                                                                                 gttggttTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCTAACCCACTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGATAGATAACTTCTTATGTCTTg                            >  2:7297086/1‑140 (MQ=38)
                                                                                                                    gtttttCTCCCTCGCGCTGTGGGTGTGGGCGGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTaa                         <  1:6460376/138‑1 (MQ=38)
                                                                                                                      tttaCTCCCTCCCCCTGGGGGAGCGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACa                       <  1:327727/140‑1 (MQ=255)
                                                                                                                         tctccctctcccTGTGGTTGTGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaa                    <  1:4480311/140‑1 (MQ=255)
                                                                                                                                  ccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGCCCCACACCCCAACCCACTACCACAAGGGAGAGGGGGCCCCTCGAGCCCCAAAATTGTGTGATATATTATTATGTGTTGTaacagaaaaacagaaa           >  2:5127833/1‑140 (MQ=16)
                                                                                                                                       tgGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccct                                                                                                      <  1:9310792/44‑1 (MQ=255)
                                                                                                                                           agagGGCCGGGGTGAGGGCATCCGCGCGCACGTTCACCCACACCCTAAACCACTCCCACAAGGGAGAGGGGACCGCTCGAGCACCAATTTTGAGTGATATCTTATTCTGTCTTGTAACAGAAGAACAGAAAATAgggggt  >  2:11258507/1‑137 (MQ=255)
                                                                                                                                           agagGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGTCCGATCGAGCACAAATTTTGAGTGATATCTTCTTATGTATTGTTACAGAAGAACAGAAAATAGGGCtt  >  2:3879867/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                           
AGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTT  >  NZ_CP025268/164254‑164532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑