Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP025268164,4120AC22.9% 226.0 / 26.9 166intergenic (+47/‑149)hrpB/mrcBATP‑dependent helicase HrpB/bifunctional glycosyl transferase/transpeptidase
Reads supporting (aligned to +/- strand):  ref base A (58/70);  new base C (3/35);  total (61/105)
Fisher's exact test for biased strand distribution p-value = 1.41e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 166 total aligned reads displayed.

AAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAA‑TACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGT‑CTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCG  >  NZ_CP025268/164274‑164550
                                                                                                                                           |                                                                                                                                           
aaaaaGAGAGGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAA‑TACTGCACCGACGCGACGGACGAAAAAGTAGTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGGGCGGGCAt                                                                                                                                            >  2:7023887/1‑140 (MQ=255)
  aaaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAA‑TACTGCACCGACGCGACGGACGAAAATGTATTCGTAAGGGTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATcc                                                                                                                                          >  1:8555770/1‑139 (MQ=255)
    agagATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAA‑TACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAgc                                                                                                                                        >  1:5312665/1‑140 (MQ=255)
      agaTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAA‑TACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCAGGGGTGAGGGCATCAgcgc                                                                                                                                      >  2:7670306/1‑140 (MQ=255)
       gaTGAATGGGCGTTATCCCAAAATTGTCTGGCCGTACGACCCGGCAAA‑TACTGCACCGACGCGACGGACGAAAAAGTATTCGTATGGTTGGTTTTCTCCCTCTCCCTGTGGGTGAGGGCCGGGGTGAGGGCATCAgcgcg                                                                                                                                     <  2:6062801/140‑1 (MQ=38)
       gaTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAA‑TACTGCACCGACGCGACGGACGAAAAATTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAgcgcg                                                                                                                                     <  2:9518371/140‑1 (MQ=255)
         tGAACGGGGTTTTTCCAAAACTTTTCTGGCCGTACGACCCGTCAAA‑TACTGAACCGACGCGACGGACGAAAATGTATTGTTTTGGTTGGTTTTCTCCCTCGCCGTGTGGGTTTGGGCCGGGGTGTGGGCCTCAGCGCGCa                                                                                                                                   <  2:1448636/140‑1 (MQ=255)
         tGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAA‑TACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCa                                                                                                                                   >  1:2114301/1‑140 (MQ=255)
         tGAAAGGGAGTTATCACAAACATGTATCGCCGGACGACCCGGCAAA‑TACTTCAACGACGCGACGGACGAAAAAGTATTCGTCAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGACGGTGTGAGTGCATCAGCGCGCa                                                                                                                                   >  1:583708/1‑140 (MQ=37)
           aaaGGGCGTTAACCCAAACATGTCGGGCCGGACGACCCGGCAAA‑TACTGCACCGCCGCGACGGACGAAAATTTTTTCGTAAGGTTGGTTTTCTCCCGCTCCCTGTGGGTGTGGGCCGGGGTGAGGGCATCAGCGCGCACg                                                                                                                                 <  2:7769882/140‑1 (MQ=37)
             tgggCGTTAGCCAAAACATGTCTGGCCGGACGACCCGGCAAA‑TACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCGCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGtt                                                                                                                               <  2:11407516/139‑1 (MQ=255)
             aGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGTCAAA‑TACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGtt                                                                                                                               <  2:10300904/140‑1 (MQ=255)
                 ggtttccccACACATGTGGGGCCGGACGCCCGGGAAAC‑TAGGGCCCCGCGGCGCCGGACGAAAAAGTATTCGTATGGTGGGTTTTCGCCCTCGCCGTGTGGGTTTGGGCGGGGGTGCGGGCCTCAGCGCGCACGTtcacc                                                                                                                           <  2:4250899/134‑1 (MQ=255)
                  gTTATCCCACACATTTCTGGCCGGACGACCCGCCAAA‑TACTCCACCGACGCGACGGACTAAAAAGTATTCTTAAGGTTGGTTTTCTCCATCTCCCTGTGGGTGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccc                                                                                                                          <  2:9918568/140‑1 (MQ=37)
                   ttATCCCAAACATGTCTGGCGGGACGCCCCGGCAAA‑TACTCCCCCGCCGCGCCGGACTAAAACTGATTCGTATGGTTGGTTTTCTCCCTCCCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccct                                                                                                                         <  2:10916258/140‑1 (MQ=25)
                   ttATCCCAAACATGTCTGGCCGTAGGACCCGGCAAT‑TACTGCACCGACGCGCCGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGTTGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccct                                                                                                                         <  2:10011989/140‑1 (MQ=38)
                   ttATCCCAAACATGTCTGGCCGGCCGACCCGGCAAA‑TACTGCACCGACGCGACGGACGAAAAAGTATTCGTACGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccct                                                                                                                         <  2:4945745/140‑1 (MQ=255)
                   ttATCCCAAACATGTCTGGCCGGACGACCCGGCAAA‑TACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccct                                                                                                                         >  1:3045521/1‑140 (MQ=255)
                   ttAACCCAAACAGGTCTGGCGGGACGACCCGGAAAA‑TACTCCCCCGACGCGACGTACGAAAATGTTTGCGTATGGTTGTTTTTCTCCCTCGCCCTGTGGGTGAGGGCCGGGGTGTGGGCCTCAGCGCGCACGTtcaccct                                                                                                                         <  2:2249742/140‑1 (MQ=255)
                   gtagcccAAACATGTCTGGCCGGACGACCCGGCAAA‑TACTCCCCCGACGCTCCTGCCGAAAAAGTATTCGTACGGTTGGTTGTCTCCCTCTCCCTGTGGGTGAGGGCCGGGGGGAGGGCATCAGCGCGCCCGTtcaccct                                                                                                                         <  2:6699310/136‑1 (MQ=255)
                          aaaCATGTCTGGCCGGACGACCCGGCAAA‑TACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGgcaccctcacccga                                                                                                                  >  2:7173423/1‑138 (MQ=255)
                          aaaCATGTCTGGCCGCACGACCCGGCAAA‑TACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTAGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAACCGCGCGCACGTTCACCCGAACCCTa                                                                                                                  >  2:7173529/1‑140 (MQ=38)
                           aaCTTGTCTGGCCGGACGACCCGGCAAA‑TACTGCACCGACGCGACGGACGAAAAAGTATTCGTATGGTTGGTTTTCGCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTaa                                                                                                                 <  2:8298143/140‑1 (MQ=255)
                           aaCATGTCTGGCCGGACGACCCGGCAAA‑TACTGCACCGCCGCGACGGTCGAATATGTCTTGGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGTGGGCGGGGGTGAGGGCAGCCGCGCGCACGGtgaccctcacccgtc                                                                                                                 >  2:11342610/1‑137 (MQ=25)
                           aaCATGTCTGGCCGGACGACCCGGCAAA‑TACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGGCACCCACACCCaaa                                                                                                                 >  2:1196248/1‑140 (MQ=255)
                           aaCATGTCGGGCCGGACGACCCGCCCAA‑TACTCCCCCGCCGCGACGGACGAAAAAGTATTCGAAAGGTTGGATTACTCCCTCCCCCTGTGGGCGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTaa                                                                                                                 <  2:9413698/140‑1 (MQ=255)
                              aTGTCTGGCCGGACGACCCGGCAAA‑TACTGCACCGACGCGACGGACGAAAATGTATTCGTAAGGTGGTTTTTCTCCCGCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAAccc                                                                                                              <  2:8900794/140‑1 (MQ=255)
                              aTGACTGGCCGGACGCCCCGCCAAA‑GCCTGCACCGCCGCGCCGGACTAAAAAGTTGTCGTACGTTTGGTTTTCGCCCACGCCGGGTGGGTGTGGGCCGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAAccc                                                                                                              <  1:2751890/140‑1 (MQ=255)
                                gTCTGGCCGTACGACCCGGAAAA‑TACGGCACCGACGCGACGGCCGAAAAAGTATTCGTATGTTTGGTTTTCTCCCTCGCCCTGTGGGAGTGGGCCGGGTTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCctc                                                                                                            <  1:10589277/140‑1 (MQ=255)
                                gTCTGGCCGGACGACCCGGCAAA‑TACTGCACCGACGCGACGGACTAAAAAGTATTCGTACGTTTGGTTTTCTCCCTCTCCCTGTGGGTGAGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCctc                                                                                                            <  2:9124021/140‑1 (MQ=38)
                                gTCTGGCCGGACGACCCGGCAAA‑TACTGCACCGACGCGACGGACTAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCctc                                                                                                            <  2:10192914/140‑1 (MQ=255)
                                gTCTGGCCGGACGACCCGGCAAA‑TACTGCACCGACGCGACGGACGAAAAATTATTCGTACGGTTGTTTTTCTCCCTCTCCCTGTGGTAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCctc                                                                                                            <  2:11374189/140‑1 (MQ=255)
                                gTCTGGCCGGACGACCCGGCAAA‑TACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCGCACCCAAAcccac                                                                                                            >  2:1279510/1‑138 (MQ=255)
                                gTCTGGCCGGACGACCCGGCAAA‑TACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCACACCCAAAcccac                                                                                                            >  2:8313336/1‑138 (MQ=255)
                                gTCTGGCCGGACGACCCGGCAAA‑TACTGCACCGACGCGACGGACGAAAAAGTATTCGGAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTGGTCCCGCACCCCAAcccac                                                                                                            >  1:9781916/1‑138 (MQ=37)
                                gTCTGGCCGCACGACCCGGCAAA‑TACTGCACCGACGCGAGGGACGAAAATGTTTTCTTATGGTTGGTTTTCTCCCTCTCCCTGTGGGTGAGGGCGGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCctc                                                                                                            <  2:3524930/140‑1 (MQ=37)
                                gTCTGCCCGGCCGCCCCGCCAAA‑TCCTGCCCCGCCGCGACGGCCCAAAACTTATTCGTACGTTGGGTTTTCGCCCCCCCCCGGTGGGCGCGGGCCGGGGTGAGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCctc                                                                                                            <  1:6185093/140‑1 (MQ=255)
                                gTCGGGCGGGACGACCCGGCAAA‑TACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCctc                                                                                                            <  2:1488865/140‑1 (MQ=255)
                                 tCTGGCCGGACGACCCGGCAAA‑TACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCAAACCctca                                                                                                           >  2:10425180/1‑139 (MQ=255)
                                  cTGGCCGGACCACCCGTCAAA‑CCCTCCACCGCCGCGACGGACCAAAATGTATTCGAACGTTGGGTTTACTCCCTCCCCCGGTGGGTTCGGGCGGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCtctc                                                                                                          <  1:9201639/140‑1 (MQ=255)
                                   gggCCGGACGACCCGGCAAA‑TACTGCACCGACGCGACGGACGAAAAAGCATGCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTcc                                                                                                         <  2:6129013/139‑1 (MQ=255)
                                    ggCCGGACGACCCGGCAAA‑TACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGTCACCCACAACCAAAACCactcac                                                                                                        >  1:3948013/1‑138 (MQ=37)
                                     gCCGGACGACCCGGAAAA‑TACGGCACCGACGCGACGGACGAAAAAGTATTCGTATGGTTGTTTTTCGCCCTCCCCCTGTGGGAGTGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCt                                                                                                       <  2:5507807/140‑1 (MQ=255)
                                        ggACGACCCGGCAAA‑TACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCTCACCCGAACCCTCACCCTCaa                                                                                                    >  2:3884224/1‑140 (MQ=255)
                                         tacgccCCGTAAAA‑TACTCCACCGCGGCGCCGGACGAAAATGTATTGTTACGGTTGGTTTTCGCCCTCCCCCGGGGGGTGAGGCCGGGGGTGAGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAg                                                                                                   <  2:2298653/139‑1 (MQ=255)
                                         gacgacCCGGCAAA‑TCCTGCACCGACGCGACGGACGACAACGTATTCTTAAGGTGGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGTCACCCTCACCCTAACCCTCTCCCTCAAg                                                                                                   <  2:10799416/140‑1 (MQ=37)
                                         gacgacCCGCAAAA‑TACGGCACCGACGCGACCGACCAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGATAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAg                                                                                                   <  2:6969735/140‑1 (MQ=38)
                                          acgacCCGGCAAA‑TACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCTCACCCTAAACCACACCCACAAgg                                                                                                  >  2:6458750/1‑140 (MQ=38)
                                           ccgaccGGCAAACGACTGCACCGCCGCGCCGGACTAAAAAGTTTTCTTTTGTGTGTTTTCCCCCCTCACCGTGGGGGTGTGGGGGGGGGTGCGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAgg                                                                                                  <  1:8313337/136‑1 (MQ=255)
                                             ccccGGCAAA‑TACTGCACCGACGCGACGGACGAAAAAGTATTCGTATGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGAATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGgag                                                                                               <  2:2919906/139‑1 (MQ=255)
                                             ccccGGCAAA‑TACGGCACCGACGCGACGGCCGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGgag                                                                                               <  2:8421800/139‑1 (MQ=255)
                                             acCCGGCAAA‑TCCGGCCCCGACGCGCCGGACGAAAACGTATTCGTATGGTTGGTTTACTCCCACACCCTGTGGGAGTGGGCGGGGGGGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGgag                                                                                               <  2:2207941/140‑1 (MQ=255)
                                             acCCGCCAAA‑TCCGGCCCCGCCGCGACGCACGAAAAAGTTTTCGAACGGTGGGTTTTCGCCCTCACCCTGGGGGAGAGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGgag                                                                                               <  2:9195714/140‑1 (MQ=255)
                                               ccGGCAAA‑TACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCACACCCAAACCCACACCCACAAGGGagag                                                                                             >  2:3759418/1‑140 (MQ=37)
                                                        tACTGCACCGACGCGACGGACGAAAAAGTATTCGTACGGTTGTTTTTCTCCCTCACCCTGTGGTAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGa                                                                                     <  1:7428609/140‑1 (MQ=39)
                                                         aCTCCACCGCCGCGACGGACGAAAAAGTCATCGTAGGTTTGTTTTTCACCCTCTCCTGGTGGGAGAGGGCCGGGTTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTACCCCTCTCCCTCAAGGGAGAGGGGACCGAt                                                                                    <  1:10057420/140‑1 (MQ=255)
                                                           tGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGACCCGCCCCCAAAACCACTCCCACAATGGAGAGGGGGCCgcaag                                                                                  >  1:10636769/1‑136 (MQ=25)
                                                             cACCGACGCGACGGACGAAAATTTATTCTTACGGTTGGTTTTCGCCATCTCCCTGGGGGTGAGGGCCGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAg                                                                                <  1:4783172/140‑1 (MQ=38)
                                                             cACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTGACCCGCACCCTAACCCACTCCCACAAGGGGGGGGGGGCCGCTCgtg                                                                                >  1:8540030/1‑138 (MQ=25)
                                                             cACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCGAACCCACTCCCACAAGGGAGAGGGGACCCATCCAg                                                                                >  2:6460375/1‑140 (MQ=37)
                                                                  acgcgacgGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccct                                                                                                                         <  2:7656495/94‑1 (MQ=255)
                                                                  acgcgacgGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccct                                                                                                                         >  1:7656496/1‑94 (MQ=255)
                                                                     cgccgtacgAAAAAGGATTCTTAAGGTTGGTTTACTCCCTCTCCCTGTGGGTGAGGGCCGGGGTGAGGGCATCAGCGCGCCCGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAtt                                                                        <  2:9066962/134‑1 (MQ=38)
                                                                            ctaaaatGTATTCGTATGGTTGGTTTTCTCCCTCTCCCGGGGGTTGAGGGCCGGGTTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgagag                                                                 <  1:9207458/138‑1 (MQ=38)
                                                                            cgAAATAGTATTCGTACGGTGGGTTTGCGCCCTCTCCCTGGGGGCGCGGGCCGGGGTTCGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgagag                                                                 <  1:6510324/140‑1 (MQ=37)
                                                                                 aaGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCGGGGGGTGAGGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtc                                                            <  1:10444018/140‑1 (MQ=39)
                                                                                  aGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCGCAAGGGAGAGGGGACCGATCGAGCACCAATTTTGTGAGATAtct                                                           >  2:1806482/1‑140 (MQ=255)
                                                                                    tctccGTACGGTGGGTGTCCTCCCTCCCCCGGTGGGAGTGGGCGGGGGTGAGGCCCTCAGCGCGCACGTGCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttc                                                         <  1:10029109/136‑1 (MQ=37)
                                                                                    tATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCAACCTCACCCAAAACCACTCCCACAAGGGAGAGGGGGCCCCGCGAGCCCAACATTTTATAGATGtcttc                                                         >  1:9524247/1‑140 (MQ=21)
                                                                                       tgttacggttggttTTCTCCCTCTCCCGGTGGGAGTGGGCGGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttcttc                                                      <  2:2110423/134‑1 (MQ=39)
                                                                                         gTATGGTTGTTTTTCACCCACTCCCTGTGGTAGTGGGCGGGGGTGAGGGACTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTg                                                    <  1:4720753/140‑1 (MQ=38)
                                                                                            tggttggttTTCCCCCACTCCCTGTGGGTGTGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGT‑Ct                                                <  1:5865365/139‑1 (MQ=39)
                                                                                                 gtttttCTCCCTCGCGCTGTGGGTGTGGGCGGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGT‑CTTGTaa                                           <  1:6460376/138‑1 (MQ=38)
                                                                                                 ggttTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGGTCACCCACACCCAGACCCGAACCCACAAGGGCGCGGGGCCCGATCGAGCCCCAATTTTGAGCGATATATTTTTCTGT‑CTGGtga                                           >  2:11147687/1‑138 (MQ=16)
                                                                                                   tttaCTCCCTCCCCCTGGGGGAGCGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGT‑CTTGTAACa                                         <  1:327727/140‑1 (MQ=255)
                                                                                                    tttCTCCCTCCCCGTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGT‑CTTGTAACag                                        <  1:1669560/140‑1 (MQ=255)
                                                                                                      tctccctctccgGGGGGGTGTGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGT‑CTTGTAACagaa                                      <  2:2548041/140‑1 (MQ=39)
                                                                                                      tctccctctcccTGTGGTTGTGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGT‑CTTGTAACagaa                                      <  1:4480311/140‑1 (MQ=255)
                                                                                                      tctccctctcccTGTGGGAGCGGGCCGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTACCCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGT‑CTTGTAACagaa                                      <  1:4718669/140‑1 (MQ=255)
                                                                                                               ccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGCCCCACACCCCAACCCACTACCACAAGGGAGAGGGGGCCCCTCGAGCCCCAAAATTGTGTGATATATTATTATGT‑GTTGTaacagaaaaacagaaa                             >  2:5127833/1‑140 (MQ=16)
                                                                                                                    tgGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccct                                                                                                                         <  1:9310792/44‑1 (MQ=255)
                                                                                                                        agagGGCCGGGGTGAGGGCATCCGCGCGCACGTTCACCCACACCCTAAACCACTCCCACAAGGGAGAGGGGACCGCTCGAGCACCAATTTTGAGTGATATCTTATTCTGT‑CTTGTAACAGAAGAACAGAAAATAgggggt                    >  2:11258507/1‑137 (MQ=255)
                                                                                                                         gcgggCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGT‑CTTGTAACAGAAGAACAGAAAATCGGGCttt                   <  1:1569198/138‑1 (MQ=255)
                                                                                                                          agGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCTAACCCACTCCCGCAAGGGGGGGGGGGCCGATAGAGGACAAATTTTGTGTGATATATTCTTCTGTAGTTGTAA‑ATAAGAACAAAAAAACGGGCgttt                  >  2:8915937/1‑140 (MQ=255)
                                                                                                                           gggCGGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGT‑CTTGTAACAGAAGAACAGAAAATCGGGCTTTTg                 <  1:8806631/140‑1 (MQ=255)
                                                                                                                           gggCGGGGGTGTGGCCCTCAGCGCGCACGTACACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGT‑CTTGTAACAGAAGAACAGAAAATCGGGCTTTTg                 <  1:3759419/140‑1 (MQ=39)
                                                                                                                           gggCCGGGGTTAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGT‑CTTGTAACAGAAGAACAGAAAATCGGGCTTTTg                 <  2:583708/140‑1 (MQ=255)
                                                                                                                           gggCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGT‑CTTGTAACAGAAGAACAGAAAATCGGGCTTTTg                 <  2:4121670/140‑1 (MQ=255)
                                                                                                                              ccGGGGTGCGGGCCTCCGCGCGCACGTTCACCCTCACCCTACCCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGT‑CTTGTAACAGAAGAACAGAAAATCGGGCTTTTgcgc              <  2:10810938/140‑1 (MQ=255)
                                                                                                                              ccGGGGTGAGGGCATCAGCGCGCACGTTGACCCTCACCCGAAACCTCTCCCTCAAGGGCGTGGGGTCCGCTAGAGCACAAATTTTGAGAGATATCTTATTCGGT‑GTTGTTCCAGTAGAACAGTGAAAAGGGCTTTTgggg              >  2:7340878/1‑137 (MQ=255)
                                                                                                                              ccGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGT‑CTTGTAACAGAAGAACAGAAAATCGGGCTTTTgcgc              <  2:3602571/140‑1 (MQ=255)
                                                                                                                              ccGGGGTGAGGGCATCAGCGCGCACGTTCACCCGCACCCAAAACCACTCCCAGAAGGGGGAGGGGCCCGATAGAGCACAAAATTTGTGATTTATATTAATATTT‑ATTGTTACCGAAGAACAGAAAAAAGGGCTTTTgagc              >  2:1883882/1‑140 (MQ=255)
                                                                                                                                ggggTGAGGGCATCAGCGCGCCCGTTCACCCTCCCCCAAAACCGCGCCCTCAAGGGAGCGGGGCCCGCTCGGGCACAAATTTTTAGTGATATCTTCTTCTGG‑AGTGTTACCCAAGAACCGACGATCGGGGTGTTGCGccg            >  2:9527517/1‑139 (MQ=255)
                                                                                                                                ggggTGAGGGCATCAGCGCGCACGTTAACCCACACCCAAAAACAATCCCCAAAGGGAGAGGGGACCGATAGAGCACAAATTTTGAGAGATATATTAATAAGT‑AATGTAACAAAATAACAGAAAATAAGGGtttttagcca            >  2:2865274/1‑134 (MQ=255)
                                                                                                                                      aGGGCATCAGCGCGCACGTGCACCCACACCCAAACCCACACCCACAAGGGGGAGGGGACCGATCGCGCACAAAATTTTTGAGATAAATTCTTATGT‑CTTGTTACAAAAAAACACAAAATCGGGCGTTTTCGCCAGAatat      >  2:2715864/1‑140 (MQ=255)
                                                                                                                                       gggCATCAGCGCGCACGTTCACCCAAACCCAAAACCACAACCACAAGGGGGGGGGGGCCCATCGCACACAAATTTTGTGAGATAAATTCATATGT‑ATTGTTACACAAGAACAGAAAATACGGCTTTTTGGCCAGAATAtt     >  2:6820941/1‑140 (MQ=255)
                                                                                                                                        ggCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTGTGAGAGATATCTTCTTCTGT‑CTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTg    <  2:5209859/140‑1 (MQ=255)
                                                                                                                                          cATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGT‑CTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg  <  1:8437920/140‑1 (MQ=255)
                                                                                                                                          cATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGT‑CTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg  <  1:498469/140‑1 (MQ=255)
                                                                                                                                          cATCAGCGCGCACGGTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGCTCGAGCACAAATTTTGAGAGATATCTTCTTCTGT‑CTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg  <  1:5618383/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
AAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAA‑TACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGT‑CTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCG  >  NZ_CP025268/164274‑164550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑