BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	360,362	0	C	A	20.5%	539.5 / 24.4	316	E184* (GAA→TAA)	lacA	galactoside O‑acetyltransferase
Reads supporting (aligned to +/- strand): ref base C (121/123); new base A (63/0); total (193/123)
Fisher's exact test for biased strand distribution p-value = 5.71e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 325 total aligned reads displayed.

TCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACG  >  NZ_CP025268/360223‑360501

tCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTc                                                                                                                                             <  1:3167227/140‑1 (MQ=255)

   tGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTcgcg                                                                                                                                          <  2:9732650/140‑1 (MQ=255)

    gCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGa                                                                                                                                         >  1:249512/1‑140 (MQ=255)

       ggATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGaaaa                                                                                                                                      >  1:63676/1‑138 (MQ=255)

       ggATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATa                                                                                                                                      >  2:3989598/1‑140 (MQ=255)

         aTGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAAc                                                                                                                                    <  2:6284386/140‑1 (MQ=255)

              gCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGAc                                                                                                                               >  1:5809603/1‑140 (MQ=255)

                   tGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAAACCGACAAGGa                                                                                                                          >  1:5871407/1‑140 (MQ=255)

                         tCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAAACCCACAAGGAACGCCa                                                                                                                    >  1:1297818/1‑140 (MQ=255)

                            cTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAgcc                                                                                                                 >  2:9407515/1‑140 (MQ=255)

                            cTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAACCCCACAAGGAACGCCAgcc                                                                                                                 >  1:4247314/1‑140 (MQ=255)

                                  ttGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCacg                                                                                                           <  1:9478968/140‑1 (MQ=255)

                                    gTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAACCCGACAAGGAACGCCAGCCGCCacgac                                                                                                         >  2:2361791/1‑140 (MQ=255)

                                    gTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGAAGAATCAACTTTATAATCTTTGAAATAATAGTGCTTATACCGGTCGTTTATTTGGCGAATAAACCGACCAAGAACGCCAGCCGCCacgac                                                                                                         >  2:106408/1‑140 (MQ=255)

                                       ggCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAACCCCACAAGGAACGCCACCCCCCACGACGtt                                                                                                      >  1:5685649/1‑140 (MQ=255)

                                         ccTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTtg                                                                                                    >  2:2256009/1‑140 (MQ=255)

                                         ccTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAAAGCCAGCCGCCACGACGTTtg                                                                                                    >  1:1935515/1‑140 (MQ=255)

                                           tGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTTTTTAGCGAATAAACCCAAAAGGAAACCCAGCCGCCACCACCTTttgt                                                                                                  >  1:6382255/1‑140 (MQ=255)

                                           tGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTAATTCGCGACTAACCCGACAAGGAACGCCAGCCGCCACGACGTTtggt                                                                                                  >  2:10051401/1‑140 (MQ=255)

                                            gATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTAATTAGCGTATAACCCGACAAGGAACGCCAGCCGCCACGACGTTtggtg                                                                                                 >  1:9913800/1‑140 (MQ=255)

                                             aTAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTtggtgg                                                                                                >  2:5429208/1‑140 (MQ=255)

                                              tAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGa                                                                                               <  2:10902355/140‑1 (MQ=255)

                                                 gcgcAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATg                                                                                            <  1:9319211/140‑1 (MQ=255)

                                                  cgcAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATATTAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGTCGTTTGGGGGAATGt                                                                                           >  2:10451057/1‑140 (MQ=255)

                                                  cgcAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGt                                                                                           >  2:4609035/1‑140 (MQ=255)

                                                  cgcAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGt                                                                                           >  2:1191413/1‑140 (MQ=255)

                                                  cgcAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGGTTGGTGGACTGt                                                                                           >  2:1578958/1‑140 (MQ=255)

                                                  cgcAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAAACCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGTATGt                                                                                           >  1:8416756/1‑140 (MQ=255)

                                                      gCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCttt                                                                                       <  2:11370196/140‑1 (MQ=255)

                                                      gCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCttt                                                                                       <  1:2229647/140‑1 (MQ=255)

                                                      gCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCttt                                                                                       <  1:3856688/140‑1 (MQ=255)

                                                       cGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAAACCGACAAGGAACGCCAGCCGCCACGAAGGTTGGTGGACTGTCtttt                                                                                      >  2:5140018/1‑140 (MQ=255)

                                                       cGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAAAAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCtttt                                                                                      >  2:1019724/1‑140 (MQ=255)

                                                          aTCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTtgtg                                                                                   <  1:1478433/140‑1 (MQ=255)

                                                          aTCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTtgtg                                                                                   <  1:1092468/140‑1 (MQ=255)

                                                           tCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGa                                                                                  <  2:7786969/140‑1 (MQ=255)

                                                            cAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAANTAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAAACCCACAAAGAACGCCACCCCCCACCACCTTATGTGTAATTTATTTTTTGaa                                                                                 >  1:10622030/1‑139 (MQ=255)

                                                            cAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGAc                                                                                 >  2:6638842/1‑140 (MQ=255)

                                                            cAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAAACCGACAAGGAACGCCAGCCGCCACGTCGGTTGGTGGAATGTCTTTTGTGAc                                                                                 >  2:3573214/1‑140 (MQ=255)

                                                            cAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCACGGTCGTTTATTTCGCGAATAACACGACAAGGAAAGCCAGCCGCCAAGACGTTTGGTGTAATGTCTTTTGTGAc                                                                                 >  2:6023704/1‑140 (MQ=255)

                                                                 aaTTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTGTTTTGCGACAAAACCCACAAGGAACGCCCGCCCCCCCGCCGTGTGGTGGAATTTCGTTtgtgtaggtaa                                                                            >  1:7816333/1‑133 (MQ=255)

                                                                 aaTTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGAtac                                                                            >  2:11116737/1‑140 (MQ=255)

                                                                   tttttATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTGTATTTAGCGAAAAACACCCCCAGGAAAGCCCGCCCCCCCCACCTGTTGTGGGATGTATTTTGTGGAGAtacta                                                                          >  1:5015008/1‑140 (MQ=255)

                                                                      ttATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTAccc                                                                       <  2:4870729/140‑1 (MQ=255)

                                                                          aaTTTAAACTGACGATTCAACTTTATAATCTTGGAACTAATAGTGCTTCTCCCGGTCGTTTATTTCGCGAATAACCCGACATGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGTCGATACTTCCCgcgc                                                                   <  2:6624489/140‑1 (MQ=255)

                                                                           aTTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGcc                                                                  <  2:7287354/140‑1 (MQ=255)

                                                                           aTTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGcc                                                                  <  1:4162669/140‑1 (MQ=255)

                                                                            tttAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCa                                                                 <  2:3532972/140‑1 (MQ=255)

                                                                              tAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAAt                                                               <  2:7612804/140‑1 (MQ=255)

                                                                                       gATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAAtt                                                      <  1:9560403/140‑1 (MQ=255)

                                                                                       gATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAAtt                                                      <  1:2079925/140‑1 (MQ=255)

                                                                                          tCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATc                                                   <  1:8652285/140‑1 (MQ=255)

                                                                                          tCAACATTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGAAGTTTGGTGGAATGTCTTTTGTGAAGATACTACACGCGCCAAAAACATAATTATc                                                   >  2:1924309/1‑140 (MQ=255)

                                                                                               tttATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGa                                              <  2:802163/140‑1 (MQ=255)

                                                                                               tttATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGa                                              <  1:4595983/140‑1 (MQ=255)

                                                                                                 tataATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAAACCGAACAGGAAACCCAGCCGCCACCACGTTTTGTGGTATTTATTTTTTGACGATAAAAACCGCGCCAATAAAAGAATTATACCCCATg                                            >  1:1650559/1‑140 (MQ=255)

                                                                                                 tataATCTTTGAAATAATAGTGCTTATCCAGGTCGTTTATTTGGCGAATAAACCGACAAGGAAAGCCAGCCGCCACCACGTTTGGTGGACTGTGTTTTGTGACGATACTACCCGCGCCAATAAAAGAATTATACCCGATg                                            >  2:314006/1‑140 (MQ=255)

                                                                                                   taATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGGCGTTTGGTGGAATGTCTTTTTTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGAAggg                                          >  2:1766863/1‑139 (MQ=255)

                                                                                                   taATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGt                                          <  1:7175084/140‑1 (MQ=255)

                                                                                                   taATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGt                                          <  2:7232772/140‑1 (MQ=255)

                                                                                                   taATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAAAAACCCCACAAGGAACCCCCGCCCCCACGCCGTGTGGTGGAAAGTCGTTTGTGTAGATAAAACCCCCCCCAAAAACAGAAATATACCCCAAGGt                                          >  1:531665/1‑140 (MQ=255)

                                                                                                      tCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGAc                                       <  1:3706421/140‑1 (MQ=255)

                                                                                                        tttGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGc                                     >  2:5899964/1‑140 (MQ=255)

                                                                                                        tttGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGCATAAACCCACCACGAACGCCCCCCCCCACCACCGTTTGTTGAATGTCTTTTTTTAAGATACAAACCCCCCCCAAAAAACAAATATACCCCAAGGTGAccc                                     >  1:6147081/1‑138 (MQ=255)

                                                                                                        tttGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAAACAGACAAGGAACGCCATCCGCCAAGACGTGTGGTGGAATTTATTTTGTTACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGAAGc                                     >  2:4440406/1‑140 (MQ=255)

                                                                                                        tttGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAAAAACCCGACAAGGAACGCCAGCCGCCACGACGGTTTGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAAAAACAGAAATATACCCCATGGTGACGc                                     >  2:3411610/1‑140 (MQ=255)

                                                                                                         ttGAAATAATAGTGCTTATCCCGGTCGTTTCTTTCGCGAATAACCCGACCAGGAACGCCACCCGCCACGACGTTGGGAGGATTGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAAGTATCCCCGATTGTGACGcc                                    >  2:6429583/1‑140 (MQ=255)

                                                                                                         ttGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAAAAACCCGACCAGGAACGCCATCCGCCACGACCTTTTGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAAAATTATCCCCCAAGGTGACGcc                                    >  2:131138/1‑140 (MQ=255)

                                                                                                         ttGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAAAAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTATTTTTTGAAGAAACTAACCGCGCCAAAAACAGAAATATCACCGATTGTGACGcc                                    >  2:1057368/1‑140 (MQ=255)

                                                                                                         ttGAAATAATAGTGCTTATCCCGGTCGTGTATTTAGCGAAAAACCCCACAAGGAACGCCACCCGCCCCCACGTTTTGTGGAATTTCTTTTGTGAAGATAATACCCGCGCCAAAAAAAGAATTATACCCGAAGGTGAAGcc                                    >  1:823687/1‑140 (MQ=255)

                                                                                                          tGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCt                                   <  1:3271801/140‑1 (MQ=255)

                                                                                                           gAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTg                                  <  1:8884104/140‑1 (MQ=255)

                                                                                                             aataatAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGa                                <  2:8665860/140‑1 (MQ=255)

                                                                                                              ataataGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGAt                               <  1:8215250/140‑1 (MQ=255)

                                                                                                                  taGTGCTTCTCCCGGTCGTTTATTTCGCGAATACCCCGACAAGGAAAGCCAGCCGCCACGACGTTTGGTGGCATGTCTTTTGTGACGATACTACCCGCGCCAATACCAGAATTATCCCCGATGGTGACGCCTGGATtaat                           <  2:2748290/140‑1 (MQ=255)

                                                                                                                  taGTGCTTATCCCGGTCGTTTATTTGGCGAATAAACCCACAATGAAACCCAGCCGCCACGACGTTTTGTGGGATTTCTTTTGTGACGATAATACCCGCGCCAAAAAAAGAAATATACCCCATGGTGACGCCGGGATtaat                           >  1:7424772/1‑140 (MQ=255)

                                                                                                                  taGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGTAACTACCCGCGCCCATACCAGAATTATCCCCGATGGTGACGCCTGGATtaat                           >  2:6473338/1‑140 (MQ=255)

                                                                                                                  taGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATtaat                           <  2:7469480/140‑1 (MQ=255)

                                                                                                                  taGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTAGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATtaat                           <  2:678649/140‑1 (MQ=255)

                                                                                                                     tGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAAc                        <  2:249512/140‑1 (MQ=255)

                                                                                                                       cTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGGTTGGTGGAATGTCTTTTGTGAGGATACTACCCCCGCCCATAACAGAATTATCCCCGCTGGTGACGCCTGGATTAATAACca                      >  2:10985439/1‑140 (MQ=255)

                                                                                                                       cTTATCCCGGTCGTTTATTTCGCGAATAACCCCACAAGGAACGCCAGCCGCCACGACGGTTTGTGGAATGTATTTTTTGACGATACAACCCGCGCCAAAAACATAATTATACCCCATGGTGACGCCAGGATtaataaacc                      >  2:924/1‑137 (MQ=255)

                                                                                                                         tATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACcaca                    <  1:10132824/140‑1 (MQ=255)

                                                                                                                          aTCCCGGTCGTTTATTTCGCGAAAAAACCCACAAGGAAACCCAGCCGCCACCAAGTTTGGTGGAATGTCATTTGTGTCCATACTACCCCCGCCAAAAACAAAAATATACCCGAAGGTGACGCCAGGATTAATAAAcacaa                   >  2:2149170/1‑139 (MQ=255)

                                                                                                                               ggTCGTTTATTTAGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATACCCGATGGTGACGCCTGGATTAATAACCACATGACtt              >  2:3760584/1‑140 (MQ=255)

                                                                                                                               ggTCGTTTAATTCGCGTATAACCCGAAAATGAACAACACCCGCCACGACGTTTGGTGGAATTTAATTTGTGACGATACTAACCGCGCCAATAACAGAAATATCCCCGATGGTTAAGCCTGGAATAATAACCACATGaaat              >  2:5286394/1‑137 (MQ=255)

                                                                                                                               ggTCGTGTATTTCGCGAATAAACCGACAAGGAACGCCAGCCGCCACGGCGGTTGGTGGAATGTCTTTTGGGACGATAATACCCGCGCCAATAACATAATTATCCCCGATGGTGACGCCTGGATTAATAACCACAAGAcgt              >  1:11320200/1‑138 (MQ=255)

                                                                                                                                gTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTc             >  2:9643665/1‑140 (MQ=255)

                                                                                                                                gTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACAAGACTTc             >  2:8432111/1‑140 (MQ=255)

                                                                                                                                gTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACAACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCAGGATTAATAACCACATGACTTc             >  2:10007441/1‑140 (MQ=255)

                                                                                                                                gTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAAAGTCTTTTGTGACGATACAACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTc             >  2:10212358/1‑140 (MQ=255)

                                                                                                                                gTCGTTTATTTAGCGAATAAACCGACAACGAACGCCAGCCGCCACGACGTTTTGTGGAAAGTGTTTTTTGAAGATAATACCCCCGCCCATAAAAAAATTATACCCCAAGGTGACGCCAGGATTAATAAACACAAGACtta             >  1:10981530/1‑139 (MQ=255)

                                                                                                                                gTCGTTTAATTCGCGAATAACCCCACACGGAACGCCAGCCGCCACGACGTTTGGTGGCCTGTCGTTTGTGACGCTCCGACCCGAGCCCATCACAGAAATATCCCCGCTGGTGACGCCTGGAGGATTCACCCCATGCCttg             >  2:8933487/1‑139 (MQ=255)

                                                                                                                                 tCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTcc            <  2:955912/140‑1 (MQ=255)

                                                                                                                                 tCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGAAGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTcc            <  2:10525867/140‑1 (MQ=255)

                                                                                                                                 tCGTTTATTTCGCGAATAACCCGACAAGGAACCCCAGCCCCCACGACGTTTGGTGGAATGTATTTTTTGACGATACTACCCCCGCCAATAAAAGAATTATACCCGATTGTGAAGCCTGGATTAATAAACACAAGACttac            >  2:6963774/1‑138 (MQ=255)

                                                                                                                                     ttATTTCGCGAATAAACCGACAAAGAACGCCAGCCGCCACGACGTTTTGTGGCAATTCTTTTGTGACGATAAAAACCCCGCCAATAAAAGAATTAAAACCCATGGTGACGCCAGGATTAATAACCACAAGACTTCCGATc        >  2:4210011/1‑140 (MQ=255)

                                                                                                                                      tATTTAGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACAACCCGCGCCAATAACATAAATAACCCCGATGGTGACGCCTGGATTAATAAACACATGACATACGAtac       >  2:2957832/1‑138 (MQ=255)

                                                                                                                                      tATTTAGCGAAAAACCCGACAAGGAACGCCAGCCGCCACGACGGTTTGTGGAATGTCTTTTGTGACGATAATAACCGCGCCAATAACAGAATTATACCCGATTGTGACGCCGGGATTAATAACCACATGAAATCCGAtac       >  2:5476133/1‑138 (MQ=255)

                                                                                                                                        tttCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAg     <  1:8045109/140‑1 (MQ=255)

TCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACG  >  NZ_CP025268/360223‑360501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`