BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	95,839	A→G	453 (TAA→TAG) I3V (ATT→GTT)	murF → mraY →	UDP‑N‑acetylmuramoyl‑tripeptide‑‑D‑alanyl‑D‑ alanine ligase phospho‑N‑acetylmuramoyl‑pentapeptide‑ transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	95,839	0	A	G	99.9%	2275.5 / NA	656	453 (TAA→TAG) I3V (ATT→GTT)	murF mraY	UDP‑N‑acetylmuramoyl‑tripeptide‑‑D‑alanyl‑D‑ alanine ligase phospho‑N‑acetylmuramoyl‑pentapeptide‑ transferase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (350/304); minor base T (0/1); total (350/306)
Fisher's exact test for biased strand distribution p-value = 4.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 656 total aligned reads displayed.

GATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCATT  >  NZ_CP025268/95705‑95974

gATAAAACTGCGTTAATTACTCGTCTTAAATTACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTtgg                                                                                                                                    <  2:11336132/140‑1 (MQ=255)

 aTAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTtggc                                                                                                                                   >  1:10303517/1‑140 (MQ=255)

 aTAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTtggc                                                                                                                                   >  1:6324216/1‑140 (MQ=255)

  tAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTtggct                                                                                                                                  >  2:9439717/1‑140 (MQ=255)

     aaCTGCGTTAATTACGCGTCTTAAATTACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTtggctggc                                                                                                                               <  1:1447137/140‑1 (MQ=255)

        tGCGTTAATTACGCGTCTTAAATTACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGa                                                                                                                            >  1:2389445/1‑140 (MQ=255)

           gTTAATTACGCGTCTTAAATTACTGATTGCTGAGCAACATGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACa                                                                                                                         >  2:391653/1‑140 (MQ=255)

           gTTAATTACGCGTCTTAAATNACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACa                                                                                                                         >  1:5978655/1‑140 (MQ=255)

           gTTAATTACGCGTCTTAAATAACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACa                                                                                                                         >  2:1692703/1‑140 (MQ=255)

            ttAATTACGCGTCTTAAATTACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACAt                                                                                                                        >  2:11015105/1‑140 (MQ=255)

                  aCGCGTCTTAAATTACTGTTTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTc                                                                                                                  <  2:2882528/140‑1 (MQ=255)

                  aCGCGTCTTAAATTACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTc                                                                                                                  <  1:11431618/140‑1 (MQ=255)

                  aCGCGTCTTAAATTACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTc                                                                                                                  <  2:1468708/140‑1 (MQ=255)

                    gcgTCTTAAATTACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCaa                                                                                                                >  1:1542542/1‑140 (MQ=255)

                    gcgTCTTAAATTACTGATTGCTGAGCAACAGGTAATTACGATTGTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCaa                                                                                                                >  1:1346954/1‑140 (MQ=255)

                         ttAAATTACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGGAGTGCCGCCATGGAAGAGGTAGTACGCGCGTTACAGGAGAATGGGACAGGTTAGTTTGGCTGGCCGAACATTTGGTCAAAtatt                                                                                                           >  1:7676800/1‑140 (MQ=255)

                               tACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCg                                                                                                     <  2:4845504/140‑1 (MQ=255)

                                aCTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCGTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATCTTATTCCgg                                                                                                    >  2:2439169/1‑140 (MQ=255)

                                  tGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCt                                                                                                  >  2:1817291/1‑140 (MQ=255)

                                     ttGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTa                                                                                               <  1:8998792/140‑1 (MQ=255)

                                      tGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTaa                                                                                              >  1:2260426/1‑140 (MQ=255)

                                       gCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAAc                                                                                             <  1:3653024/140‑1 (MQ=255)

                                           aGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCt                                                                                         >  1:3435151/1‑140 (MQ=255)

                                                cAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTATcc                                                                                    >  2:3451948/1‑140 (MQ=255)

                                                     aaTTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTCCAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCt                                                                               >  2:65224/1‑140 (MQ=255)

                                                     aaTTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCt                                                                               >  1:10101651/1‑140 (MQ=255)

                                                          cGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGt                                                                          >  1:6973344/1‑140 (MQ=255)

                                                          cGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGt                                                                          >  2:8200877/1‑140 (MQ=255)

                                                            aTTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGttt                                                                        <  2:3142957/140‑1 (MQ=255)

                                                             ttttAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATTTTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTc                                                                       >  1:1961447/1‑140 (MQ=255)

                                                             agatAGTTGACGGGTCACGTCGTGCCGCACTGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGTCTGGCCGAACATTTGGAACAATATTATTCCTGCGATAACGTCTTTTCCGATCTGACGttta                                                                       >  2:2760740/4‑139 (MQ=255)

                                                              tttAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTcg                                                                      >  2:4571959/1‑140 (MQ=255)

                                                                tAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTcgcg                                                                    <  2:7930701/140‑1 (MQ=255)

                                                                 aGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCgcgc                                                                   <  1:5364974/140‑1 (MQ=255)

                                                                     aaGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGTGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTcgcg                                                                    >  2:11069105/1‑135 (MQ=255)

                                                                            cACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGcc                                                        >  1:6479759/1‑140 (MQ=255)

                                                                            cACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACAGTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGcc                                                        >  1:973296/1‑140 (MQ=255)

                                                                              cGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCctg                                                      <  2:1227999/140‑1 (MQ=255)

                                                                                 aGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCctgctg                                                   >  1:10901292/1‑140 (MQ=255)

                                                                                  gTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGa                                                  <  1:8125389/140‑1 (MQ=255)

                                                                                  gTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGa                                                  <  2:4345463/140‑1 (MQ=255)

                                                                                   tGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGAc                                                 >  1:1913091/1‑140 (MQ=255)

                                                                                    gccgccATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGAcc                                                >  1:9849859/1‑140 (MQ=255)

                                                                                     ccgccATGGCAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTGTCCTATGTGACGTTTCGCGCCATCGTCAGCCTGCTGACcg                                               <  2:5805542/140‑1 (MQ=255)

                                                                                     ccgccATGGATGAGGTAGTACGCGCTTTACTGGAGATTGGGACATGTTAGTTTGGATGGCCGAACATTTGGTCAATTATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACcg                                               <  2:670916/140‑1 (MQ=255)

                                                                                       gccTTGGAAGAGGGAGTACGCGCTTTACAGGACAATGGGACATCTTAGTTTGGCTGGCCGAACAATTGGTCAAATATTAATCCGGCTTTAACGTCTTTTCCTAACTGACGTTTCGCGCCATCGTCAGCCTGCTGACcgcg                                             <  2:8847635/140‑1 (MQ=255)

                                                                                       gccATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACcgcg                                             <  1:8342806/140‑1 (MQ=255)

                                                                                       gccATGGAAGAGGTAGTACGCGCTTGACAGGAGAATGGGACATGTGAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACcgcg                                             <  2:7080921/140‑1 (MQ=255)

                                                                                       gccATGGAAGAGGTAGTACGCGCTGTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACcgcg                                             <  1:10459435/140‑1 (MQ=255)

                                                                                           tGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGt                                         <  2:5210911/140‑1 (MQ=255)

                                                                                           tGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGt                                         <  2:4081797/140‑1 (MQ=255)

                                                                                           tGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGt                                         <  1:4283425/140‑1 (MQ=255)

                                                                                             gAAGAGGTAGTACGCGCTTTACAGGAGACTGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTc                                       >  2:2126783/1‑140 (MQ=255)

                                                                                             gAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTc                                       >  2:4973873/1‑140 (MQ=255)

                                                                                             gAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTc                                       >  1:5757241/1‑140 (MQ=255)

                                                                                             gAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTc                                       >  2:1994946/1‑140 (MQ=255)

                                                                                               tgagGTAGTCCGCCCATTCCACGAGCATGTGACATCTTTGTTTTGCTGGCAGAACATTTGGTCAAATATTATTCCGTCTTTAACGTCTTTTCCTATCTTCAGTATCGCGCCCTCGTCCTCCTGCTGACCGCGCTGTTCag                                     <  2:6364753/139‑2 (MQ=255)

                                                                                               agagGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCAt                                     <  1:3083012/140‑1 (MQ=255)

                                                                                               agagGTAGTACGCGCCTTACAGGAGAAAGGGACATGTTAGATTGGCTGGCCGAACAATTGGTCAAATAATATTCCGGCTTTAACGTCTTTTCCTCTCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCAt                                     <  1:5249241/140‑1 (MQ=255)

                                                                                                   gtagtaCGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCa                                 >  2:5722847/1‑140 (MQ=255)

                                                                                                   gtagtaCGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCa                                 >  2:8974074/1‑140 (MQ=255)

                                                                                                   gtagtaCGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCa                                 <  1:7394458/140‑1 (MQ=255)

                                                                                                   gtagtaCGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCa                                 >  1:3853304/1‑140 (MQ=255)

                                                                                                     agtaCGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCAtt                               >  1:6192965/1‑140 (MQ=255)

                                                                                                     agtaCGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCAtt                               <  1:7472789/140‑1 (MQ=255)

                                                                                                     agtaCGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCAtt                               >  1:3588167/1‑140 (MQ=255)

                                                                                                       taCGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATtgt                             >  2:9311744/1‑140 (MQ=255)

                                                                                                       taCGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATtgt                             >  1:9476564/1‑140 (MQ=255)

                                                                                                       taCGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATtgt                             >  1:10627056/1‑140 (MQ=255)

                                                                                                        aCGCGCTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATtgtg                            >  1:7868430/1‑140 (MQ=255)

                                                                                                          gcgcTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGa                          >  1:7163245/1‑140 (MQ=255)

                                                                                                           cgcTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGAt                         >  2:3780245/1‑140 (MQ=255)

                                                                                                             cTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATgg                       >  1:2972876/1‑140 (MQ=255)

                                                                                                             cTTTACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATgg                       >  2:6063183/1‑140 (MQ=255)

                                                                                                              tttACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCGAGCGTCAGCCTGCTGACCGCGCTGTTGATCTCATTGTGGATggg                      >  2:10737187/1‑140 (MQ=255)

                                                                                                              tttACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCCTCTCAATGTGGATggg                      >  2:6216987/1‑140 (MQ=255)

                                                                                                              tttACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATggg                      >  2:10488198/1‑140 (MQ=255)

                                                                                                               ttACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGc                     <  2:5586844/140‑1 (MQ=255)

                                                                                                               ttACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGc                     <  1:4543282/140‑1 (MQ=255)

                                                                                                               ttACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGc                     <  2:6962109/140‑1 (MQ=255)

                                                                                                               ttACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGc                     <  2:7746281/140‑1 (MQ=255)

                                                                                                               ttACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGc                     <  1:8759883/140‑1 (MQ=255)

                                                                                                               ttACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGc                     <  2:8064999/140‑1 (MQ=255)

                                                                                                               ttACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGc                     <  2:8304202/140‑1 (MQ=255)

                                                                                                               ttACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTAACCGCGCTGTTCATCTCATTGTGGATGGGc                     <  1:9176814/140‑1 (MQ=255)

                                                                                                               ttACAGGAGAATGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTAGTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGc                     <  2:5886434/140‑1 (MQ=255)

                                                                                                                     gagaAGGGGACATGTTAGTGTGGCTGGCCGAACATTTGGTCAAAGATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGt               <  2:10060167/140‑1 (MQ=255)

                                                                                                                        aaagggACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGACTTTTCCTATCTGACGATTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATg            >  2:1610991/4‑140 (MQ=255)

                                                                                                                        aaagggACATGTTAGTATGGCTGGCAGAAAATTTGGTAAAATAATATTCCGGCTTTAACGTCTTTACCTATATGACGTTTCGAGCCATCGTCAGCCAGCAGACCGCGCAGTTCATAACACTGTGGATGGGCCCGCGAATg            >  2:9656790/4‑140 (MQ=255)

                                                                                                                        aaTGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATg            >  1:2804763/1‑140 (MQ=255)

                                                                                                                        aaTGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATg            >  1:9350409/1‑140 (MQ=255)

                                                                                                                        aaTGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATg            >  1:4882985/1‑140 (MQ=255)

                                                                                                                          tGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGAt          >  2:3587221/1‑140 (MQ=255)

                                                                                                                          tGGGACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGAt          >  2:1402866/1‑140 (MQ=255)

                                                                                                                            ggACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTg        >  1:6787392/1‑140 (MQ=255)

                                                                                                                            ggACATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTg        >  2:8597349/1‑140 (MQ=255)

                                                                                                                               cATGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTc     >  1:9607436/1‑140 (MQ=255)

                                                                                                                                aTGTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCa    <  2:7369329/140‑1 (MQ=255)

                                                                                                                                  gTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCCtt  >  2:102758/1‑140 (MQ=255)

                                                                                                                                  gTTAGTTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCAtt  >  1:4073369/1‑140 (MQ=255)

GATAAAACTGCGTTAATTACGCGTCTTAAATTACTGATTGCTGAGCAACAGGTAATTACGATTTTAGTTAAGGGTTCACGTAGTGCCGCCATGGAAGAGGTAGTACGCGCTTTACAGGAGAATGGGACATGTTAATTTGGCTGGCCGAACATTTGGTCAAATATTATTCCGGCTTTAACGTCTTTTCCTATCTGACGTTTCGCGCCATCGTCAGCCTGCTGACCGCGCTGTTCATCTCATTGTGGATGGGCCCGCGTATGATTGCTCATT  >  NZ_CP025268/95705‑95974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`