Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 1,292,848 A→T noncoding (11/132 nt) CXP41_RS06830 ← RtT sRNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252681,292,8480AT76.5% 378.0 / 212.3 414noncoding (11/132 nt)CXP41_RS06830RtT sRNA
Reads supporting (aligned to +/- strand):  ref base A (62/30);  new base T (57/242);  total (135/279)
Fisher's exact test for biased strand distribution p-value = 2.75e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 419 total aligned reads displayed.

GATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTC  >  NZ_CP025268/1292709‑1292987
                                                                                                                                           |                                                                                                                                           
    attTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTggg                                                                                                                                         <  1:6211667/140‑1 (MQ=14)
    attTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTggg                                                                                                                                         <  2:6144995/140‑1 (MQ=14)
      ttAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCa                                                                                                                                       <  1:1772424/140‑1 (MQ=11)
      ttAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCa                                                                                                                                       <  1:760608/140‑1 (MQ=11)
      ttAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCa                                                                                                                                       <  2:2926560/140‑1 (MQ=11)
           gacgCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAata                                                                                                                                  <  2:10540866/137‑1 (MQ=255)
              gCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATAttt                                                                                                                               <  2:5591106/140‑1 (MQ=11)
               cGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGACGAACCTTGGTCGAAGCTTCTCATCCATCCCCGCTTGGGCAGACTAttgg                                                                                                                              >  2:10836394/1‑138 (MQ=11)
                                         aGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGTCGGCAACGCTCTCAAGATGGCGCTCAAAACGAAACGTGGTGGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGGGTGTGAATTCCgg                                                                                                    >  2:5369952/1‑140 (MQ=11)
                                         aGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCCACCCGCTCTCGCGGGCGCTCAAGTCGAACCGTGGTCGAAGCTTCTCAACCATCCCCGCGTGGGGAGCAGAGTTGATTGCGGATTCGTGTGAGAATTcccg                                                                                                    >  1:8107381/1‑138 (MQ=2)
                                          gcgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCggg                                                                                                   <  1:6727908/140‑1 (MQ=11)
                                          gcgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCggg                                                                                                   <  1:8587358/140‑1 (MQ=11)
                                          gcgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCGTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCggg                                                                                                   <  2:2564211/140‑1 (MQ=11)
                                           cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCgggg                                                                                                  <  1:8358579/140‑1 (MQ=11)
                                                cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGTATTCCGGGGCtttt                                                                                             <  1:2606596/140‑1 (MQ=11)
                                                cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCtttt                                                                                             <  1:11114854/140‑1 (MQ=11)
                                                cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCtttt                                                                                             <  2:10239851/140‑1 (MQ=11)
                                                cgcgcTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGCTCTCTCGCTGGCGCTCCAGTCGAACCGTGGTCGAAGCTTCTCATCCTTCCCCGCGTGGGCAGTATATTTGATTGCGGATTCGGTTGTGACTTGCGGGGCtttt                                                                                             >  2:3741504/1‑140 (MQ=11)
                                                   gcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGaa                                                                                          <  1:4328975/140‑1 (MQ=11)
                                                   gcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGaa                                                                                          <  1:10165195/140‑1 (MQ=11)
                                                     ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGGTTGAGAATTCCGGGGCTTTTGAAAg                                                                                        >  2:4060751/1‑140 (MQ=11)
                                                     ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCAACCTTCCCCGCTTGGGCAGAATATTATATAGCGGAGGCGTTTGAGAAGTACGGGGCTTTTGAAAg                                                                                        >  2:716715/1‑140 (MQ=11)
                                                     ttCGCCCTTCGGGTCGTTGCCGGCGGCAACGCTCTCTCGCTGGCGCTCAAGGCGACCCGTGGGCGAAGCTTCTCATCCGTGCCCGCGTGGGGAGAATATTTGATGGCGGCTTAGGTTGAGAATTCCGGGGCTTTTGAAAg                                                                                        >  1:11220159/1‑140 (MQ=11)
                                                        gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGa                                                                                     <  1:2897035/140‑1 (MQ=11)
                                                        gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGGCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCGTGGGCAGAATATTTGATTGCGGATTCGTGTGAGAATTCCGGGGCTTTTGACAGGGa                                                                                     >  2:9701074/1‑140 (MQ=11)
                                                           cTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGAtgg                                                                                  <  1:743984/140‑1 (MQ=11)
                                                              cGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGAtggtgg                                                                               <  1:1267591/140‑1 (MQ=11)
                                                               gggTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCTAAGCTACACATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGATTGAGAAGTCCGGGGCTTTTGAAAGTGAtggtggt                                                                              <  2:7043646/140‑1 (MQ=11)
                                                               gggTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGAtggtggt                                                                              <  2:8240768/140‑1 (MQ=11)
                                                               gggTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGAtggtggt                                                                              <  1:773641/140‑1 (MQ=11)
                                                                  tCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCGTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTggg                                                                           <  1:5579110/140‑1 (MQ=11)
                                                                    gTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCAAGCCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGATTGAGAATTCCGGGTCTTTTGAAAGTGATGGTGGTggggg                                                                         <  1:3681782/140‑1 (MQ=11)
                                                                    gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCTAGTCGAACGTTGGTCGAAGCTTATCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTggggg                                                                         <  1:10851164/140‑1 (MQ=11)
                                                                    gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTggggg                                                                         <  1:6361834/140‑1 (MQ=11)
                                                                    gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTggggg                                                                         <  2:4557446/140‑1 (MQ=11)
                                                                    gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTggggg                                                                         <  1:4807683/140‑1 (MQ=11)
                                                                    gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTggggg                                                                         <  1:3163163/140‑1 (MQ=11)
                                                                    gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTggggg                                                                         <  1:3881254/140‑1 (MQ=11)
                                                                    gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTAGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTggggg                                                                         <  1:7300374/140‑1 (MQ=11)
                                                                         cTGCGGCAACGCTCTCTCGCTGGCGCTCACGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGa                                                                    <  1:66333/140‑1 (MQ=11)
                                                                         cTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGa                                                                    <  1:6552387/140‑1 (MQ=11)
                                                                         cTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGa                                                                    <  2:836136/140‑1 (MQ=11)
                                                                         cTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGa                                                                    <  1:8814258/140‑1 (MQ=11)
                                                                         cTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGa                                                                    <  1:6027280/140‑1 (MQ=11)
                                                                                   gCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAgcg                                                          <  2:343217/140‑1 (MQ=14)
                                                                                     tctctcGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCt                                                        <  1:4040626/140‑1 (MQ=14)
                                                                                      ctctcGCTGGCGCTCAAGTCGAACCGTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATGGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTg                                                       <  2:7642371/140‑1 (MQ=14)
                                                                                        ctcGCTGCCGCTCGAGCCGAACCTTAGACGAAGCTTCTCATCCGTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTgcg                                                     <  2:10682949/140‑1 (MQ=11)
                                                                                            cTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCt                                                 >  1:9508551/1‑140 (MQ=14)
                                                                                             tgtcgcTCGTGCCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTAGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCtt                                                <  2:8604533/137‑1 (MQ=11)
                                                                                             tGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGACTATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCtt                                                <  2:3117872/140‑1 (MQ=14)
                                                                                              ggCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTc                                               <  2:9341591/140‑1 (MQ=14)
                                                                                              ggCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCCGCTCTACGAGCTTc                                               >  2:6773741/1‑140 (MQ=11)
                                                                                              gacgatCGAGCCGAACCTTAGTCGAAGCTTCTCAGCCTTCCCCGCATGGGCAGATTATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTc                                               <  2:7808965/135‑1 (MQ=11)
                                                                                               acgcTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCg                                              <  1:4500744/139‑1 (MQ=255)
                                                                                               acgcTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGAGCTGCGCGCTTCg                                              >  1:6863666/2‑140 (MQ=11)
                                                                                                cgcTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGc                                             <  2:10419228/140‑1 (MQ=255)
                                                                                                 gcTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGACTATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCGGCGCGCTTCGcc                                            >  1:2457828/1‑140 (MQ=11)
                                                                                                 gcTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGcc                                            >  1:4704075/1‑140 (MQ=14)
                                                                                                  cTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGccc                                           >  1:10419229/1‑140 (MQ=14)
                                                                                                   tcaagtcGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCt                                          >  1:5137611/7‑140 (MQ=14)
                                                                                                    cacgtcGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCtt                                         <  2:7526700/135‑1 (MQ=14)
                                                                                                     aagtcGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTc                                        <  2:2410516/136‑1 (MQ=14)
                                                                                                     aagtcGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTc                                        <  2:4256932/136‑1 (MQ=14)
                                                                                                     aagtcGAACCTTGGTCGAAGCTTCTCATCCGTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTc                                        <  1:8387126/136‑1 (MQ=14)
                                                                                                     aagtcGAACCTTGGTCGAAGCTTCTCATCCGTCACCGCTTTGGCAGAATATTTGATTGCGGATTCGTTTGATAATTCCTGGGCTTTTGAAAGTGATGGTTGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTc                                        <  2:4947534/136‑1 (MQ=14)
                                                                                                          gAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCGTGGGCAGAATTTGTGATTGCGGATTCGTGTGAGAAGTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTc                                   <  2:2229355/140‑1 (MQ=14)
                                                                                                           aaCCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCg                                  >  2:2482291/1‑140 (MQ=255)
                                                                                                           aaCCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCg                                  >  1:10574782/1‑140 (MQ=255)
                                                                                                             ccTTAGTCGAAGCTTCTCCTCCGTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCTGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGtt                                <  2:8824559/140‑1 (MQ=11)
                                                                                                               ttggtCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGACAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGc                              <  1:156339/136‑1 (MQ=255)
                                                                                                               ttggtCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGc                              >  1:2283047/5‑140 (MQ=255)
                                                                                                               ttggtCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGc                              >  2:521348/5‑140 (MQ=255)
                                                                                                                tggtCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGcc                             <  2:6952448/137‑1 (MQ=255)
                                                                                                                tggtCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGcc                             <  2:1434977/137‑1 (MQ=255)
                                                                                                                 agtCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCt                            >  1:10011124/3‑140 (MQ=255)
                                                                                                                 agtCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCt                            >  2:1554870/3‑140 (MQ=255)
                                                                                                                  gtCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGGCGTTGCCTg                           >  1:9435822/2‑140 (MQ=255)
                                                                                                                     gAAGCTTCTCATCCGTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCgg                        <  1:8750600/140‑1 (MQ=255)
                                                                                                                       agattCTCACCCTTACACGCTTTGCCAGATTATATGATTGCGGATTCGTTTGAGTATTCCCTGTCTTTAGTAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCa                      <  1:5696660/137‑1 (MQ=14)
                                                                                                                        gCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCaa                     >  2:1011109/1‑140 (MQ=255)
                                                                                                                        gCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGAGCTGAGAGCTTCGCCCTTCGGGTCGTTGCATGCGGCaa                     >  2:5815580/1‑140 (MQ=11)
                                                                                                                        gCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACACAGCGCTGCGCGCATCGCCCGTCGGGTCGTTGCCTGCGGCaa                     >  2:5909545/1‑140 (MQ=11)
                                                                                                                        gCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTGTGAAAGGGATGGTGGTGGGGGACGGATTACTCCGCGCTGCGCGCTTCGCCCTGCGGGTCGTGGCCTGCGGCac                     >  1:9119085/1‑139 (MQ=11)
                                                                                                                        gCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTAGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCaa                     >  1:5759896/1‑140 (MQ=255)
                                                                                                                         cTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTATGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAAc                    <  1:9834136/140‑1 (MQ=255)
                                                                                                                           tctcATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTTCGCTCATCGCCCTTCGGGTCGTTGCCTGAGGCAACGc                  >  2:177018/1‑140 (MQ=11)
                                                                                                                            ctcATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGct                 <  1:7993168/140‑1 (MQ=255)
                                                                                                                                   ttCCCCGCTTGGGCAGAATATGTGATTGCGGATTCGTGTGAGACTTCCGGGGCTTTTGACAGTGATGGTGGTGGGGGAAGGCTTACTCAGCGCTGCGCGCTTCGCCGTTCGGGGCGTTGCCTGCGGCAACGCTCTCTCGc          >  2:6281009/1‑140 (MQ=14)
                                                                                                                                   ttCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGGCGTTGCCTGCGGCAACGCTCTCTCGc          >  2:6633057/1‑140 (MQ=255)
                                                                                                                                    tCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGCTCTCTCGCt         <  2:1521593/140‑1 (MQ=255)
                                                                                                                                    tCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGGTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTAGTCAGAGCTGCGAGCTTCTCGCTTCGGGTCGTTGCATGAGGCAAAGCGcgctcgct         >  2:11333592/1‑140 (MQ=14)
                                                                                                                                    tCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCTGGTCGTTGCCTGAGGCAACGCTCTCTCGCt         >  2:3837681/1‑140 (MQ=11)
                                                                                                                                    tCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCt         <  1:2035563/140‑1 (MQ=255)
                                                                                                                                    tCCCCGATTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCt         <  2:6063063/140‑1 (MQ=255)
                                                                                                                                    gccccGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAAGTCCGGTGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGATTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCt         <  2:8985169/139‑1 (MQ=14)
                                                                                                                                     ccccGCATGGGCAGAATCGTTGATTGCGGAGTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGACGGAGTACTCAGCGCGGCGCGCTTCGCCCTTCGGGTCGTGGCCGGCGGCGACGCTCTCTCgggg        >  1:1711383/1‑137 (MQ=11)
                                                                                                                                      cccGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTACGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTgg       >  1:11441163/1‑139 (MQ=255)
                                                                                                                                         gCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCACCGCTCTCTCGCTGgcgc    >  2:7276578/1‑140 (MQ=11)
                                                                                                                                           aTGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTAGTTTCATGAGGCAACGCACTCTCGCTGgcgcgc  >  1:10729907/1‑138 (MQ=11)
                                                                                                                                           |                                                                                                                                           
GATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTC  >  NZ_CP025268/1292709‑1292987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑