BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,423,978	A→G	163 (TAA→TAG) S51S (AGT→AGC)	sieB → ydaF ←	superinfection exclusion B family protein YdaF family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,423,978	0	A	G	99.4%	2719.8 / NA	788	163 (TAA→TAG) S51S (AGT→AGC)	sieB ydaF	superinfection exclusion B family protein YdaF family protein
Reads supporting (aligned to +/- strand): ref base A (0/1); major base G (277/504); minor base T (3/2); total (280/508)
Fisher's exact test for biased strand distribution p-value = 3.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 788 total aligned reads displayed.

ATGAAAAAATTAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGA  >  NZ_CP025268/1423841‑1424114

aTGAAAAAATTAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCt                                                                                                                                        <  2:11102801/140‑1 (MQ=255)

aTGAAAAAATTAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCt                                                                                                                                        <  2:6574616/140‑1 (MQ=255)

aTGAAAAAATTAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCt                                                                                                                                        <  2:8249411/140‑1 (MQ=255)

aTGAAAAAATTAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCt                                                                                                                                        <  2:9340517/140‑1 (MQ=255)

  gAAAAAATTAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTa                                                                                                                                      <  1:6298304/140‑1 (MQ=255)

  gAAAAAATTAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTa                                                                                                                                      <  2:9164896/140‑1 (MQ=255)

  gAAAAAATTAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTa                                                                                                                                      <  1:7800332/140‑1 (MQ=255)

  gAAAAAATTAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTa                                                                                                                                      <  1:2591274/140‑1 (MQ=255)

  gAAAAAATTAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGACACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTa                                                                                                                                      <  2:10023796/140‑1 (MQ=255)

   aaaaaaTTAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTAc                                                                                                                                     >  1:3440175/1‑140 (MQ=255)

    aaaaaTTAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACt                                                                                                                                    <  2:5564372/140‑1 (MQ=255)

    aaaaaTTAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACt                                                                                                                                    <  1:11082713/140‑1 (MQ=255)

    aaaaaTTAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACt                                                                                                                                    <  1:9111313/140‑1 (MQ=255)

    aaaaaTTAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACt                                                                                                                                    <  2:9286594/140‑1 (MQ=255)

    aaaaaTTAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACt                                                                                                                                    <  2:1153114/140‑1 (MQ=255)

     aaaaTTAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACtg                                                                                                                                   <  1:7583390/140‑1 (MQ=255)

       aaTTAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACtgtg                                                                                                                                 <  1:3613260/140‑1 (MQ=255)

       aaTTAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACtgtg                                                                                                                                 <  2:3205176/140‑1 (MQ=255)

        aTTAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACtgtgt                                                                                                                                <  2:8059144/140‑1 (MQ=255)

        aTTAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACtgtgt                                                                                                                                <  1:612431/140‑1 (MQ=255)

        aTTAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACtgtgt                                                                                                                                <  2:10238693/140‑1 (MQ=255)

        aTTAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAAGCGTTAGCTTACtgtgt                                                                                                                                >  2:1787343/1‑140 (MQ=255)

         ttAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACtgtgtg                                                                                                                               <  1:1862286/140‑1 (MQ=255)

         ttAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACtgtgtg                                                                                                                               <  1:5016174/140‑1 (MQ=255)

         atAGAACGGACGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACtgtgtg                                                                                                                               <  1:8175721/139‑1 (MQ=255)

           aGAACGGAATGGAATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCG‑AAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTATAATCGTTAGCTTACTGTGAGcgt                                                                                                                            <  2:3767346/140‑3 (MQ=255)

           aGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCt                                                                                                                             <  2:8506026/140‑1 (MQ=255)

           aGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCt                                                                                                                             <  1:4360244/140‑1 (MQ=255)

            gAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCtt                                                                                                                            <  2:716139/140‑1 (MQ=255)

            gAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCtt                                                                                                                            <  2:4577471/140‑1 (MQ=255)

              aaggAAGGTCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCACATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTtct                                                                                                                          <  2:7319284/138‑1 (MQ=255)

                           atTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGgcg                                                                                                             <  2:9079601/140‑1 (MQ=255)

                            ttCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGgcgc                                                                                                            <  2:1920251/140‑1 (MQ=255)

                            ttCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGgcgc                                                                                                            >  1:10018415/1‑140 (MQ=255)

                            ttCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGgcgc                                                                                                            >  2:6125333/1‑140 (MQ=255)

                               caCATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAAAACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCa                                                                                                         <  1:10446947/139‑1 (MQ=255)

                                    cAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGGGAGCTTACTGTGTGCTTCTCCCACCATCGGCGCGCACCAGt                                                                                                    >  2:8648635/1‑140 (MQ=255)

                                    cAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAAGCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGt                                                                                                    >  1:7870896/1‑140 (MQ=255)

                                       agTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTc                                                                                                 >  1:2346872/1‑140 (MQ=255)

                                       agTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTc                                                                                                 >  1:8502769/1‑140 (MQ=255)

                                       agTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTc                                                                                                 >  2:4193141/1‑140 (MQ=255)

                                           aTAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGACCAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGtt                                                                                             >  1:3053402/1‑140 (MQ=255)

                                           aTAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCTTCGGCGCGCACCAGTTTCGGtt                                                                                             >  1:6930211/1‑140 (MQ=255)

                                           aTAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGtt                                                                                             >  2:5738654/1‑140 (MQ=255)

                                               cgcAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTaa                                                                                         <  2:11301150/140‑1 (MQ=255)

                                               cgcAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTaa                                                                                         >  2:9408982/1‑140 (MQ=255)

                                               cgcAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTaa                                                                                         >  1:9688723/1‑140 (MQ=255)

                                                 cAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTGTTAATCGTTAGCTTACTGTGTGCTTCTCCCACCAACGGCGCGCACCCGTGTCGGTTTTAAAt                                                                                       >  2:10399931/1‑140 (MQ=255)

                                                    aCTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTAGGTTTTAAATGtt                                                                                    <  1:9835415/140‑1 (MQ=255)

                                                      tGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGtttt                                                                                  <  1:7253224/140‑1 (MQ=255)

                                                          cTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCtt                                                                              <  2:7550351/140‑1 (MQ=255)

                                                           ttattaTCTCTTCACCGAAAAATAATCCAATTTTATGAAGTTATTATGTAACAGCATGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACAATCTGAGAGCACAATTTTCGGTTTTAAATGTTTTGAttt                                                                             <  2:8795376/140‑1 (MQ=255)

                                                           ttattaTCTCGTCACCGAAAAATACTCCCATTTTATGACGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCttt                                                                             <  1:1593502/140‑1 (MQ=255)

                                                           ttattaTCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCttt                                                                             <  2:7015922/140‑1 (MQ=255)

                                                                atCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGta                                                                        >  1:4125968/1‑140 (MQ=255)

                                                                atCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGta                                                                        <  2:4404947/140‑1 (MQ=255)

                                                                atCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGta                                                                        <  2:3642852/140‑1 (MQ=255)

                                                                atCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGta                                                                        <  1:3302881/140‑1 (MQ=255)

                                                                atCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGta                                                                        <  2:5325455/140‑1 (MQ=255)

                                                                atCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGta                                                                        <  2:4951684/140‑1 (MQ=255)

                                                                  ctcGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGtata                                                                      >  2:4676072/1‑140 (MQ=255)

                                                                  ctcGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTCTTGGtata                                                                      <  2:8950585/140‑1 (MQ=255)

                                                                  ctcGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTGTCGGTTTTAAATGTTTTGCTTTTGGtata                                                                      >  2:6352858/1‑140 (MQ=255)

                                                                  ctcGTCACCGAAAAATACTCCCATTTTATGAAGTTAGTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGtata                                                                      <  1:665984/140‑1 (MQ=255)

                                                                    cgtaaaagAAATATACTCACATTTTATGTAGTTATTCTGGATAAGCTGGATTAGTCGTTTTAATAGTTAGCTTACTGTGTGCTTCTCCACCCATCTGCGCGCAGCAGTTTCGGTTTTATATGTTTTGCTTTTGGTATACg                                                                    <  2:3432506/133‑1 (MQ=255)

                                                                     gTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGt                                                                   >  2:3073508/1‑140 (MQ=255)

                                                                     gTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGt                                                                   <  1:1282158/140‑1 (MQ=255)

                                                                      tCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCAGCAGTTTCGGTTTTAAATGTTGTGCTGTTGGTATACTTc                                                                  >  2:7935948/1‑140 (MQ=255)

                                                                       cACCGAAAAATACTCCCATTTTATGAGGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCa                                                                 >  1:10155786/1‑140 (MQ=255)

                                                                       cACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCa                                                                 <  1:2115206/140‑1 (MQ=255)

                                                                        aCCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGta                                                                        <  1:5787049/132‑1 (MQ=255)

                                                                            aaaaaTACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCa                                                            <  2:2788603/140‑1 (MQ=255)

                                                                            aaaaaTACTCCCATATTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCa                                                            <  1:8715329/140‑1 (MQ=255)

                                                                               aaTACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATTTTTTGCTTTTGGTATACGTCATGGCAGTg                                                         >  1:10825537/1‑140 (MQ=255)

                                                                                acaCTACCATTTGATGAAGGTAATCAGGACCACCAGGAGTAGCCGCTAAAATCGTTATCTTACTGTGTGCTACTCCAACCCTCGGAGCGCCCCAGTTTCGGTTTTAAATGTTTTGCTTGTGGTATACGTCATGGCAGTGa                                                        <  2:9726469/138‑1 (MQ=255)

                                                                                    tCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGt                                                    <  1:11359176/140‑1 (MQ=255)

                                                                                         ttttATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCAt                                               >  2:2420867/1‑140 (MQ=255)

                                                                                              tGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGTCAGTGAACGTTCCATCCTgg                                          <  1:7120164/140‑1 (MQ=255)

                                                                                                aaGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGtt                                        >  1:943252/1‑140 (MQ=255)

                                                                                                  gTTATTCTGGACCAGCAGGAGTCGACGTTTTAATCGTGAGCTTAGTGTGTGCTTATCCAACCATCGGCGCGCACCAGGTGCGGTATTATATGTTTTGCTGTTGGTATACGTCATGGAAGTGAACGTTCCATCCGGGTTgg                                      >  2:6771291/1‑140 (MQ=255)

                                                                                                     aTTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGa                                   >  1:320696/1‑140 (MQ=255)

                                                                                                          ggAACAGCAGGTGTAGTCGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCTGTGAACGTTCCATCCTGGTTGGGGAACAcg                              <  1:10955514/140‑1 (MQ=255)

                                                                                                            aaCAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACAcgcc                            <  2:2186521/140‑1 (MQ=255)

                                                                                                             aCAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACAcgccg                           <  2:10745905/140‑1 (MQ=255)

                                                                                                             aCAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACAcgccg                           <  1:5547478/140‑1 (MQ=255)

                                                                                                                gcagGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGcac                        <  2:4780660/140‑1 (MQ=255)

                                                                                                                gcagGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGcac                        >  2:4028111/1‑140 (MQ=255)

                                                                                                                gcagGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGcac                        <  1:485390/140‑1 (MQ=255)

                                                                                                                ccagGAGTAGACGTTTTAATCGTTCGCTTACTGGTTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTATATTTTTTGCTTTTGGTATACTTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGcac                        <  2:10602332/139‑1 (MQ=255)

                                                                                                                   ggAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACAcc                     >  1:5235361/1‑140 (MQ=255)

                                                                                                                    gAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCa                    <  2:1393963/140‑1 (MQ=255)

                                                                                                                          aCGTTTTAATAGTTAGCTTACTGTGTGCTTCTCCAACCCTCTGCGCGCACCAGTTTCGGTTGTAACTGTTTTGCTTTTGGTATAAGTCATGGCAGTGAACGTTCCATCGTGGTTGGGGAACACGCCGCACACCAGGGAtt              >  2:2560083/1‑140 (MQ=255)

                                                                                                                           cGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTc             >  1:3785378/1‑140 (MQ=255)

                                                                                                                            gTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCg            >  1:4728082/1‑140 (MQ=255)

                                                                                                                              tttAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCt                                            >  1:9596375/1‑106 (MQ=255)

                                                                                                                              tttAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCgtt          >  1:2868100/1‑140 (MQ=255)

                                                                                                                              tttAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGGTTTGc                                                                                >  1:10658429/1‑70 (MQ=255)

                                                                                                                               ttAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCgttg         >  2:9830929/1‑140 (MQ=255)

                                                                                                                                     gTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCg   <  2:5895099/140‑1 (MQ=255)

                                                                                                                                      ttAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGa  >  2:5104847/1‑140 (MQ=255)

ATGAAAAAATTAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGA  >  NZ_CP025268/1423841‑1424114

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`