BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,660,166	T→C	K109R (AAG→AGG)	ynfA ←	YnfA family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,660,166	0	T	C	99.5%	1947.1 / NA	561	K109R (AAG→AGG)	ynfA	YnfA family protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (269/289); minor base A (3/0); total (272/289)
Fisher's exact test for biased strand distribution p-value = 1.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 561 total aligned reads displayed.

TAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCG‑C‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACC  >  NZ_CP025268/1660029‑1660302

taAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTa                                                                                                                                          >  2:1336036/1‑140 (MQ=255)

    gATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTg                                                                                                                                      <  1:4373890/140‑1 (MQ=255)

     aTCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTgc                                                                                                                                     >  2:10089397/1‑140 (MQ=255)

     aTCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTgc                                                                                                                                     >  2:1131806/1‑140 (MQ=255)

     aTCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTgc                                                                                                                                     >  2:8849619/1‑140 (MQ=255)

     aTCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTgc                                                                                                                                     >  2:610085/1‑140 (MQ=255)

       cGAAAACAGCCGGAGTGCTCACAAAACAAGGCGTAGAGTAGCGCTGAGAATGGTAGACAAAAGAGGAAAGAGCATACCATTTGTCAGCTGACACTTCCTCTTATCGTATTGATAAAAGGGATTTATGGTCCTACGTgcg‑c                                                                                                                                  <  2:3741975/140‑1 (MQ=255)

       cGAAAAAAGCCGGAGTTATCACAAAAAAAGGCTTATATTTGCGCTGTGAAGGGTTGACAAAATATGAAATAGAATACATTTTGTCAGCTGACACTTAATCTTATCTTATTGATAAAATGGATTTATTTTCCTAAGTgcg‑c                                                                                                                                  >  2:4745602/1‑140 (MQ=255)

       cGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTgcg‑c                                                                                                                                  >  1:6510240/1‑140 (MQ=255)

        gAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑c‑c                                                                                                                                >  1:3379181/1‑140 (MQ=255)

          aaaaaGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑c‑ccc                                                                                                                              <  2:7406633/140‑1 (MQ=255)

               gCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑c‑ccccagcc                                                                                                                         >  1:2460519/1‑135 (MQ=255)

                 cGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCg                                                                                                                        <  1:9929641/140‑1 (MQ=255)

                 cGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCg                                                                                                                        <  1:10484380/140‑1 (MQ=255)

                    aGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGACTACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGcaa                                                                                                                     <  1:5006182/140‑1 (MQ=255)

                    aGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGcaa                                                                                                                     <  1:836974/140‑1 (MQ=255)

                        aTCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATCGAAGACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTCCGTGCGCC‑CCCAGCCCGCAACAAt                                                                                                                 >  2:7965251/1‑140 (MQ=255)

                              aaaaaaGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCaa                                                                                                           >  1:4609610/1‑140 (MQ=255)

                              aaaaaaGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAAAAGCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGCTTTATGGTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATaaa                                                                                                           >  2:9588445/1‑140 (MQ=255)

                                  aaGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAAGGATCAACATg                                                                                                       >  1:8831715/1‑140 (MQ=255)

                                    ggCGTATATTTGCGATGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGcc                                                                                                     >  1:3234759/1‑140 (MQ=255)

                                        tataTTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCaa                                                                                                 <  1:7861017/140‑1 (MQ=255)

                                             ttGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAgcgc                                                                                            <  2:4951775/140‑1 (MQ=255)

                                              tGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCa                                                                                           <  2:2311657/140‑1 (MQ=255)

                                               gcgcTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCaa                                                                                          >  2:9316052/1‑140 (MQ=255)

                                               gcgcTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCaa                                                                                          >  2:1736228/1‑140 (MQ=255)

                                                cgcTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAAt                                                                                         >  1:7957900/1‑140 (MQ=255)

                                                  cTGTGAATGGTTGACAAAAGATGAACTAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCAACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCa                                                                                       >  2:2711396/1‑140 (MQ=255)

                                                  cTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCa                                                                                       <  1:640669/140‑1 (MQ=255)

                                                   tgttAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCaa                                                                                      <  1:3130799/140‑1 (MQ=255)

                                                    gtgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAAc                                                                                     >  1:4240315/1‑140 (MQ=255)

                                                     tgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTTATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACg                                                                                    >  2:9060726/1‑140 (MQ=255)

                                                     tgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACg                                                                                    >  2:9925276/1‑140 (MQ=255)

                                                     tgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACg                                                                                    <  2:2634711/140‑1 (MQ=255)

                                                        aTGGTTGACAAAAGATGAAATAGAATACCTATTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCAc                                                                                 <  1:172685/140‑1 (MQ=255)

                                                           gTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCg                                                                              <  2:165493/140‑1 (MQ=255)

                                                             tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                            <  1:5417221/140‑1 (MQ=255)

                                                             tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                            <  1:919274/140‑1 (MQ=255)

                                                              gACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTcc                                                                           >  2:4004370/1‑140 (MQ=255)

                                                               tcAAAAGATGAAATATAATACCTTTTGTCAGCTGACACTGCCTCTTAACATATTGATAAACTTGAGTTATGGTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCACCATGCCGCAAAGCGCCATCAACGCACCCGTCCa                                                                          <  2:10869437/139‑1 (MQ=255)

                                                               aCAAAAGATGTAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                          >  1:1243646/1‑140 (MQ=255)

                                                               aCAAAAGATGAAATAGAATANCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCNACATGCCNCAAAGCGCAATCAACGCACCCGTCCa                                                                          >  1:5977937/1‑140 (MQ=255)

                                                               aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                          >  2:6522069/1‑140 (MQ=255)

                                                               aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                          >  2:8480961/1‑140 (MQ=255)

                                                               aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                          >  2:972705/1‑140 (MQ=255)

                                                               aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                          >  1:2905602/1‑140 (MQ=255)

                                                               aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                          >  2:6993634/1‑140 (MQ=255)

                                                                    aGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCAt                                                                     >  1:5221121/1‑140 (MQ=255)

                                                                      aTGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATac                                                                   >  1:7397545/1‑139 (MQ=255)

                                                                      aTGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATaa                                                                   >  1:4070352/1‑140 (MQ=255)

                                                                           aTAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGt                                                              <  1:972802/140‑1 (MQ=255)

                                                                           aTAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGt                                                              <  2:3250840/140‑1 (MQ=255)

                                                                           aTAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGt                                                              <  1:11128563/140‑1 (MQ=255)

                                                                                aTACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGtt                                                         <  1:367128/140‑1 (MQ=255)

                                                                                  aCCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTc                                                       <  1:10295544/140‑1 (MQ=255)

                                                                                    cTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATTTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATAAACAAGCCGCAAAGCGCAATCAACGCACCCGTCACGTCATAAAGAGTCAGttgaac                                                     >  2:6351491/1‑136 (MQ=255)

                                                                                     ttttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAACGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACg                                                    <  1:3545756/140‑1 (MQ=255)

                                                                                     ttttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACg                                                    <  2:314544/140‑1 (MQ=255)

                                                                                     ttttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACg                                                    <  2:3543328/140‑1 (MQ=255)

                                                                                      tttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCAGCAAAGCTCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGc                                                   >  2:10659770/1‑140 (MQ=255)

                                                                                             gCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCcac                                            <  2:4397881/140‑1 (MQ=255)

                                                                                              cTGACACTTCCTCTTCTCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATACAGAGTCAGTTTCACGCCATCcaca                                           <  1:902429/140‑1 (MQ=255)

                                                                                              cTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCcaca                                           <  2:5644149/140‑1 (MQ=255)

                                                                                                 acacTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAAcg                                        <  2:5819486/140‑1 (MQ=255)

                                                                                                 acacTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAAcg                                        >  1:11401619/1‑140 (MQ=255)

                                                                                                   acgtCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAAcgcg                                      <  1:6228423/137‑1 (MQ=255)

                                                                                                    cTTCCTCTTATCTGATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACgcgc                                     >  2:872259/1‑140 (MQ=255)

                                                                                                       ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAACGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                  <  1:10694431/140‑1 (MQ=255)

                                                                                                       ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                  <  1:7137783/140‑1 (MQ=255)

                                                                                                       ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                  <  1:2707819/140‑1 (MQ=255)

                                                                                                       ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                  <  1:2065756/140‑1 (MQ=255)

                                                                                                       ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                  >  1:7602129/1‑140 (MQ=255)

                                                                                                       ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                  >  1:1099197/1‑140 (MQ=255)

                                                                                                       ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACACGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                  <  2:5203819/140‑1 (MQ=255)

                                                                                                        ctctTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGcc                                 >  2:10811830/1‑140 (MQ=255)

                                                                                                        ctctTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTGCACGCCATCCACAACGCGCAGcc                                 >  1:6800668/1‑140 (MQ=255)

                                                                                                         tcttatcttaTTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCca                                <  1:5475335/140‑1 (MQ=255)

                                                                                                         tcttatcataTTGATAAAATGGATTTATGTTCCAACGTGCG‑CACCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCca                                <  2:10344013/140‑1 (MQ=255)

                                                                                                           ttatcttatTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCCTCCACAACGCGCAGCcaca                              >  1:10951615/1‑140 (MQ=255)

                                                                                                             atcttatTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATc                            >  2:7514018/1‑140 (MQ=255)

                                                                                                               cttatTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCaa                          >  2:10425901/1‑140 (MQ=255)

                                                                                                               cttatTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCaa                          >  2:8262231/1‑140 (MQ=255)

                                                                                                                 tatTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACg                        >  1:6885295/1‑140 (MQ=255)

                                                                                                                 tatTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACg                        <  2:2099505/140‑1 (MQ=255)

                                                                                                                 tatTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACg                        <  2:1166301/140‑1 (MQ=255)

                                                                                                                 tatTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACg                        >  1:8550482/1‑140 (MQ=255)

                                                                                                                  atTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGc                       >  2:3002491/1‑140 (MQ=255)

                                                                                                                  atTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGc                       >  2:6782283/1‑140 (MQ=255)

                                                                                                                  atTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGc                       >  1:1483077/1‑140 (MQ=255)

                                                                                                                   ttGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGcc                      >  1:2195431/1‑140 (MQ=255)

                                                                                                                      aTAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTg                   <  1:10836487/140‑1 (MQ=255)

                                                                                                                      aTAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTg                   <  2:2499455/140‑1 (MQ=255)

                                                                                                                       tAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGc                  >  1:10037168/1‑140 (MQ=255)

                                                                                                                        aaaaTGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCa                 >  1:576137/1‑140 (MQ=255)

                                                                                                                             ggATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACAt            <  2:4609609/140‑1 (MQ=255)

                                                                                                                                tttATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAAACAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCATCCACATCAACGCCGTGCAGACATaaa         >  2:5971913/1‑140 (MQ=255)

                                                                                                                                  tATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACg       <  1:8090197/140‑1 (MQ=255)

                                                                                                                                  tATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACg       <  1:9459017/140‑1 (MQ=255)

                                                                                                                                  tATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACg       <  1:6676657/140‑1 (MQ=255)

                                                                                                                                   aTGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGc      >  2:1890996/1‑140 (MQ=255)

TAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCG‑C‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACC  >  NZ_CP025268/1660029‑1660302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`