BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,514,921	A→G	419 (TAA→TAG)	yfeO →	ion channel protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,514,921	0	A	G	99.5%	2597.6 / NA	750	419 (TAA→TAG)	yfeO	ion channel protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (398/347); minor base T (0/4); total (398/352)
Fisher's exact test for biased strand distribution p-value = 4.78e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 750 total aligned reads displayed.

GCGGCAGTCGTTGTACCCAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACAATGTTCAGGTCACTGTTTCTGG  >  NZ_CP025268/2514784‑2515057

gcggcAGTCGTTGTACCCAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCa                                                                                                                                        >  2:981541/1‑140 (MQ=255)

     aGTCGTTGTACCCAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCaaaaaa                                                                                                                                   <  1:2066436/140‑1 (MQ=255)

         gTTGTACCCAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGct                                                                                                                               <  1:6990417/140‑1 (MQ=255)

         gTTGTACCCAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGct                                                                                                                               <  1:10820642/140‑1 (MQ=255)

         gTTGTACCCAATACCACAGTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGct                                                                                                                               <  2:3483401/140‑1 (MQ=255)

          ttGTACCCAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGctc                                                                                                                              <  1:9093126/140‑1 (MQ=255)

                 cAATACCACATTGCTACCGCTGCTCTGTTTCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTa                                                                                                                       <  2:6879683/140‑1 (MQ=255)

                 cAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTa                                                                                                                       <  1:2700138/140‑1 (MQ=255)

                  aaTACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTAt                                                                                                                      <  2:4336714/140‑1 (MQ=255)

                    tACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGATCTCCTTTATGa                                                                                                                    >  2:3002637/1‑140 (MQ=255)

                    tACCACATTGCTACCGCTGCTCTGTAACGTCATGCTTCCGGCATCGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCAGAAGCAACAGCCACCCCACGATAACTTATAGCAAAAAATGCTCTCATtgatga                                                                                                                    >  1:6118343/1‑140 (MQ=255)

                       cacaTTGCTACCGCTGCTCTGTATCGTCTTTCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTGATGAttt                                                                                                                 <  2:10845132/140‑1 (MQ=255)

                       cacaTTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGAttt                                                                                                                 <  2:6180466/140‑1 (MQ=255)

                       cacaTTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGAttt                                                                                                                 >  1:10584586/1‑140 (MQ=255)

                       cacaTTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGAGGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGAttt                                                                                                                 >  1:8615380/1‑140 (MQ=255)

                         caTTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTaa                                                                                                               >  2:5879579/1‑140 (MQ=255)

                         caTTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTGTATGATTTaa                                                                                                               >  1:3215481/1‑140 (MQ=255)

                          aTTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGACGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAag                                                                                                              >  2:8116686/1‑140 (MQ=255)

                            tGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAagag                                                                                                            >  2:10211805/1‑140 (MQ=255)

                            tGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGAGTTAagag                                                                                                            >  1:2834022/1‑140 (MQ=255)

                            tGCTACCGCTGCTATGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCACAAAATGCTCTCCTGTATGATTTAagag                                                                                                            >  1:9832986/1‑140 (MQ=255)

                              cTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGtt                                                                                                          >  1:5082264/1‑140 (MQ=255)

                              cTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGtt                                                                                                          >  2:2257856/1‑140 (MQ=255)

                              cTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGtt                                                                                                          >  2:5746571/1‑140 (MQ=255)

                                aCCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTAt                                                                                                        <  2:11105218/140‑1 (MQ=255)

                                aCCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATGAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACTATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTAt                                                                                                        <  2:6021534/140‑1 (MQ=255)

                                 ccGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATg                                                                                                       >  1:4502814/1‑140 (MQ=255)

                                   gctgctCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGc                                                                                                     <  2:3193660/140‑1 (MQ=255)

                                    ctgctCTGTATCGTCATGCTTCCGGCTTGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCg                                                                                                    <  2:7740887/140‑1 (MQ=255)

                                    ctgctCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCg                                                                                                    >  2:11389660/1‑140 (MQ=255)

                                    ctgctCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCg                                                                                                    >  2:3727568/1‑140 (MQ=255)

                                    ctgctCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCg                                                                                                    >  1:6214437/1‑140 (MQ=255)

                                    ctgctCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCg                                                                                                    >  2:5165200/1‑140 (MQ=255)

                                      gctCTGTATCGTCATGCTTCCGGCATGGCTGTTATGAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCCCACAATGCTCTCCTTTATGATTTAAGAGTTATGGCgat                                                                                                  <  2:1810316/140‑1 (MQ=255)

                                        tctGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCgatga                                                                                                >  1:8769815/1‑140 (MQ=255)

                                          gggaTCGTCATGCTTCCGGCATGGCTGTTATTCGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATa                                                                                              <  2:9575104/137‑1 (MQ=255)

                                          ggTATCGTCCTGCTTCCGGCATGGCTGTTATTAGCAGGTACGCCGAGGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATa                                                                                              <  1:10345482/139‑1 (MQ=255)

                                           gTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATAc                                                                                             <  1:5880712/140‑1 (MQ=255)

                                           gTATCGTCATGCTTCCGGCATGGCCGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATAc                                                                                             <  2:4763172/140‑1 (MQ=255)

                                             aTCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACgg                                                                                           <  1:10955914/140‑1 (MQ=255)

                                             aTCGTCATGCTACCGGCATGGCTGATATGAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACgg                                                                                           <  1:11260113/140‑1 (MQ=255)

                                                 tCATGCTTCCGGCATGGCTGTTCATAGCAGGAAAGCCGATGATCATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATCCTCTCCATTATGATTTATGAGTTATGTCGATGATACGGGGCt                                                                                       >  2:7929263/1‑140 (MQ=255)

                                                 tCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGTTGATGGTAAATCGTCCGAATCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCATTTATGATGTAAGAGTTATGGCGATGATACGGGGCt                                                                                       >  1:771692/1‑140 (MQ=255)

                                                 tCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAACAAATGCTCTCCTGTATGATTTAAGAGTTATGGCGATGATACGGGGCt                                                                                       >  2:7612961/1‑140 (MQ=255)

                                                 tCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCt                                                                                       >  1:7201989/1‑140 (MQ=255)

                                                 tCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCt                                                                                       >  2:9166811/1‑140 (MQ=255)

                                                 tCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATGTAAGAGTTATGGCGATGATACGGGGCt                                                                                       >  1:9216699/1‑140 (MQ=255)

                                                    tGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTa                                                                                    >  2:8297975/1‑140 (MQ=255)

                                                    tGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTa                                                                                    >  1:2239617/1‑140 (MQ=255)

                                                     gCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTCGCAAAAAATGCTCTCCTTTATGATTTAAGAGGTATGGCGATGATACGGGGCTTTAc                                                                                   <  1:803453/140‑1 (MQ=255)

                                                      cTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTAcc                                                                                  >  2:6428779/1‑140 (MQ=255)

                                                       ttCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCt                                                                                 <  2:4122825/140‑1 (MQ=255)

                                                       ttCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCt                                                                                 <  1:3793902/140‑1 (MQ=255)

                                                         ccGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTcc                                                                               >  1:3322302/1‑140 (MQ=255)

                                                                ggCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAat                                                                        <  2:6624214/140‑1 (MQ=255)

                                                                 gCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGTGTTAAGAGGTATGGCGATGATCCGGGGCTTTACCGCCCCGTCata                                                                       >  1:8923703/1‑140 (MQ=255)

                                                                 gCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAata                                                                       >  1:5645433/1‑140 (MQ=255)

                                                                 gCTGTTATTAGCAGGTAAGCCAATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAata                                                                       <  1:6791346/140‑1 (MQ=255)

                                                                  cTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAatat                                                                      <  2:1295623/140‑1 (MQ=255)

                                                                    gTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTCAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTg                                                                    <  2:8457424/140‑1 (MQ=255)

                                                                           gCAGGTAAGCCGATGATGATGGTCAATCGTACTAAGCAACAGCCACGCAACGATAACGATTAGCAAAAAATGCTCTCCATTATGATTTAAGAGATATTGAGATGATAAGTGTCTTTACCTCCCCGTAATATTGCTTTAAc                                                             >  2:10588090/1‑140 (MQ=255)

                                                                            cAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACa                                                            >  1:1894540/1‑140 (MQ=255)

                                                                            cAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACa                                                            >  1:5535374/1‑140 (MQ=255)

                                                                            cAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGAAATATTGCGTTAACa                                                            >  2:10387985/1‑140 (MQ=255)

                                                                             aGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAg                                                           >  1:8495392/1‑140 (MQ=255)

                                                                               gTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGc                                                         >  1:10029957/1‑140 (MQ=255)

                                                                               gTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGc                                                         >  1:7494173/1‑140 (MQ=255)

                                                                               gTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGGAATATTGCGTTAACAGGc                                                         >  1:308256/1‑140 (MQ=255)

                                                                                    ccGATGATGAGGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAATTGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTg                                                    <  2:7204316/140‑1 (MQ=255)

                                                                                         gatgatgGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGCTTAGCAAAAAATGCTCTCCTGTATGATTTAAGAGTTATGGCGAGGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGc                                               >  1:4019293/1‑140 (MQ=255)

                                                                                          atgatgGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCt                                              >  1:3448027/1‑140 (MQ=255)

                                                                                            gatgGTCAATCGTCCGAAGCAACAGCCACCCCACGATCACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTc                                            <  1:1170198/140‑1 (MQ=255)

                                                                                            gatgGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTc                                            <  1:5390294/140‑1 (MQ=255)

                                                                                             atgGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCg                                           >  1:4391785/1‑140 (MQ=255)

                                                                                             atgGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCg                                           >  2:11179722/1‑140 (MQ=255)

                                                                                              tgGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGa                                          >  1:9540222/1‑140 (MQ=255)

                                                                                                gTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGc                                        >  2:2109567/1‑140 (MQ=255)

                                                                                                 tCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCg                                       <  2:9012312/140‑1 (MQ=255)

                                                                                                   aaTCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTGTATGATTAAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAAGATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTg                                     >  2:1926314/1‑140 (MQ=255)

                                                                                                    aTCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGa                                    <  1:3669302/140‑1 (MQ=255)

                                                                                                    aTCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGa                                    <  2:4913215/140‑1 (MQ=255)

                                                                                                    aTCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGa                                    >  2:2670061/1‑140 (MQ=255)

                                                                                                    aTCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGATTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGa                                    <  2:1677749/140‑1 (MQ=255)

                                                                                                      cGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAg                                  <  1:1406900/140‑1 (MQ=255)

                                                                                                       gTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAgg                                 >  1:6305737/1‑140 (MQ=255)

                                                                                                           gAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGaa                             <  2:8918048/140‑1 (MQ=255)

                                                                                                               cAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAAc                         >  1:1583209/1‑140 (MQ=255)

                                                                                                                aaCAGCCACCCCACGATAACGTTTAGTAAAAAATGCTCTCCATTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACa                        <  1:8150053/140‑1 (MQ=255)

                                                                                                                  cAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACAAt                      >  2:453679/1‑140 (MQ=255)

                                                                                                                    gCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACAATGt                    <  1:2377490/140‑1 (MQ=255)

                                                                                                                       aCCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACAATGTTCa                 >  1:9955451/1‑140 (MQ=255)

                                                                                                                       aCCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGAGTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATACCAATGTTCa                 >  1:10494219/1‑140 (MQ=255)

                                                                                                                         cccACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACg                                                                                            >  1:94168/1‑63 (MQ=255)

                                                                                                                         cccACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACAATGTTCAgg               >  1:7410772/1‑140 (MQ=255)

                                                                                                                         cccACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACAATGTTCAgg               >  2:7451996/1‑140 (MQ=255)

                                                                                                                         cccACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTGAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACCGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACAATGTTCAgg               >  2:9378542/1‑140 (MQ=255)

                                                                                                                         cccACGATAACGTTTAGCAAAAAATGCTCTCCTTTAGGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATAGTGCGTTAACAGGCCGCTGACTGCTTCGAGCGGGAAGGAGAATAACAATGTTCAgg               >  2:7338277/1‑140 (MQ=255)

                                                                                                                               aTAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACAATGTTCAGGTCACTg         >  1:2482184/1‑140 (MQ=255)

                                                                                                                                   cGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTAc                                                                                   <  2:249427/62‑1 (MQ=255)

                                                                                                                                     tttAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACAATGTTCAGGTCACTGTTTCTg   >  2:552024/1‑140 (MQ=255)

GCGGCAGTCGTTGTACCCAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACAATGTTCAGGTCACTGTTTCTGG  >  NZ_CP025268/2514784‑2515057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`