Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 247,291 A→G *250* (TAA→TAG)  yafL → C40 family peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP025268247,2910AG99.5% 2095.7 / NA 609*250* (TAA→TAG) yafLC40 family peptidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (260/346);  minor base T (1/2);  total (261/348)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 609 total aligned reads displayed.

GTGTATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAG‑AC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGCCA  >  NZ_CP025268/247154‑247424
                                                                                                                                         |                                                                                                                                       
gtgtATTTGGGCGATGGGCCATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑a                                                                                                                                      >  2:7808456/1‑140 (MQ=255)
gtgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑a                                                                                                                                      <  2:4840068/140‑1 (MQ=255)
 tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGANTTTGACGGAAGAGACGATTTTGTAGG‑Ac                                                                                                                                     >  1:6015947/1‑140 (MQ=255)
 tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTGTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑Ac                                                                                                                                     >  1:7745985/1‑140 (MQ=255)
 tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATGTTGTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATGTTGTAGG‑Ac                                                                                                                                     >  1:9701715/1‑140 (MQ=255)
 tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATGAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑Ac                                                                                                                                     >  2:8518563/1‑140 (MQ=255)
  gtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑AC‑g                                                                                                                                   >  2:5608467/1‑140 (MQ=255)
     tttGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑AC‑ggat                                                                                                                                <  1:5057403/140‑4 (MQ=255)
     cttGGGCGCTGGGCAAGTTATCGAGTCGCCACGTACCGGCGACACCACGCGGATAAGCCGATTCGCCGAACCTTGCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGCCGGAAGAGACGATTTTGTCGG‑AC‑ggat                                                                                                                                <  2:6208755/139‑4 (MQ=255)
       tGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑AC‑ggataa                                                                                                                              <  1:8909681/140‑6 (MQ=255)
       tGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑AC‑ggataa                                                                                                                              <  2:1435453/140‑6 (MQ=255)
       tGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑AC‑ggataa                                                                                                                              <  1:3719115/140‑6 (MQ=255)
        gggCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAg                                                                                                                              >  1:9556569/1‑140 (MQ=255)
        gggCGATGGGCAAGTTCTCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAg                                                                                                                              >  2:9311587/1‑140 (MQ=255)
             aTGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGCTGTTGTAGG‑ACGGATAAGGCGtt                                                                                                                         >  2:6396545/1‑140 (MQ=255)
             aTGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGtt                                                                                                                         >  1:1144170/1‑140 (MQ=255)
             aTGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGtt                                                                                                                         >  2:8025874/1‑140 (MQ=255)
             aTGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGtt                                                                                                                         >  1:4884493/1‑140 (MQ=255)
               gggCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTa                                                                                                                       >  1:9197339/1‑140 (MQ=255)
               gggCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTa                                                                                                                       >  2:286032/1‑140 (MQ=255)
                   aaattctCGAGTCGCCACGTACCGGCGAAACCATGCGGAGAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCATGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATACGGCGTTTAcgcc                                                                                                                   <  1:7497798/134‑1 (MQ=255)
                    aTTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTAcgccg                                                                                                                  <  2:8393412/140‑1 (MQ=255)
                      ttATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCa                                                                                                                <  1:5658679/140‑1 (MQ=255)
                      ttATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCa                                                                                                                <  1:10123207/140‑1 (MQ=255)
                        aTCGAGTCGCCACGTACCGGCGAAATCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATc                                                                                                              >  2:7601009/1‑140 (MQ=255)
                          cGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCg                                                                                                            >  1:327890/1‑140 (MQ=255)
                           gcgTCGCCACGTCCCGGCGCACCCCGTCGGAGAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTGTGGGCGCGCGCAGGATTGTGACGGAAGAGACGATTGTGTCGG‑CCGGATAAGGCGTTTACGCCGCATCCgg                                                                                                           <  2:4327169/138‑1 (MQ=25)
                           gAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGCCGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCgg                                                                                                           <  1:674741/140‑1 (MQ=255)
                           gAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCgg                                                                                                           >  2:1910152/1‑140 (MQ=255)
                           gAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCgg                                                                                                           <  2:4006400/140‑1 (MQ=255)
                           gAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTGTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCgg                                                                                                           <  2:2289218/140‑1 (MQ=255)
                              tCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAg                                                                                                        <  1:2455867/140‑1 (MQ=255)
                              tCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAg                                                                                                        <  2:3320741/140‑1 (MQ=255)
                              tCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAg                                                                                                        <  2:2181213/140‑1 (MQ=255)
                              tCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAg                                                                                                        <  1:6180167/140‑1 (MQ=255)
                                gCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGtt                                                                                                      >  2:5821319/1‑140 (MQ=255)
                                  cACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGt                                                                                                    <  1:8025875/140‑1 (MQ=255)
                                   aCGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTa                                                                                                   >  2:717278/1‑140 (MQ=255)
                                     gTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACg                                                                                                 <  1:5303827/140‑1 (MQ=255)
                                     gTACCGGCGAAACCATTCGGAGAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACg                                                                                                 <  1:995603/140‑1 (MQ=255)
                                       aCCGGCGAAACAATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCa                                                                                               <  1:4108969/140‑1 (MQ=255)
                                            cGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGc                                                                                          >  1:8219187/1‑140 (MQ=255)
                                            cGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGc                                                                                          >  1:3041484/1‑140 (MQ=255)
                                               aaCCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTg                                                                                       >  2:10787990/1‑140 (MQ=255)
                                                aCCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGAAG‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGa                                                                                      >  2:2544439/1‑140 (MQ=255)
                                                  cATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGACGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATg                                                                                    <  1:7368289/140‑1 (MQ=255)
                                                  cATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATg                                                                                    <  1:1773874/140‑1 (MQ=255)
                                                     tCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGa                                                                                 >  1:2042599/1‑140 (MQ=255)
                                                      cGGATAAGCCGATTAGCCGAAACTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTGGTACGCAGGTGCCTGATGCGAc                                                                                <  1:8750167/140‑1 (MQ=39)
                                                       ggATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACg                                                                               <  1:5240716/140‑1 (MQ=255)
                                                             gCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgcg                                                                         >  1:10648578/1‑140 (MQ=255)
                                                                gATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGt                                                                      <  1:7808457/140‑1 (MQ=255)
                                                                gATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGt                                                                      <  1:9039425/140‑1 (MQ=255)
                                                                 aTTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGTCGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTc                                                                     >  1:3394850/1‑140 (MQ=255)
                                                                    aGCCGAACCTTTCTGGCAGGACGATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGAAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTa                                                                  <  2:8194195/140‑1 (MQ=37)
                                                                    aGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTa                                                                  <  2:10727703/140‑1 (MQ=255)
                                                                    aGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGAATTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTa                                                                  <  2:10292568/140‑1 (MQ=255)
                                                                     gCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTAt                                                                 <  1:1383845/140‑1 (MQ=255)
                                                                      ccGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATc                                                                <  1:9374736/140‑1 (MQ=255)
                                                                        gAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACTCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCAt                                                              <  2:5119067/140‑1 (MQ=255)
                                                                        gAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCAt                                                              <  1:10413852/140‑1 (MQ=255)
                                                                        gAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCAt                                                              >  1:8378970/1‑140 (MQ=255)
                                                                        gAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCAt                                                              <  1:6857930/140‑1 (MQ=255)
                                                                              ttCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTAc                                                        >  1:2579562/1‑140 (MQ=255)
                                                                               tCTGGCAGGAACATTTTTTGGGCGCGCGCAGGATTTTGACTGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACg                                                       <  2:645183/140‑1 (MQ=25)
                                                                                cTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGa                                                      <  1:10937550/140‑1 (MQ=255)
                                                                                cTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGa                                                      >  1:11114327/1‑140 (MQ=255)
                                                                                cTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGa                                                      >  2:7446434/1‑140 (MQ=255)
                                                                                      ggACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCcgc                                                >  2:9195272/1‑140 (MQ=255)
                                                                                      ggACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCcgc                                                >  2:8595506/1‑140 (MQ=255)
                                                                                      ggACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCcgc                                                >  2:3110843/1‑140 (MQ=255)
                                                                                      ggACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCcgc                                                >  2:6026330/1‑140 (MQ=255)
                                                                                      ggACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCcgc                                                <  1:4635117/140‑1 (MQ=255)
                                                                                      ggACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCcgc                                                >  1:550589/1‑140 (MQ=255)
                                                                                        aCCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGa                                              >  2:10516658/1‑140 (MQ=255)
                                                                                        aCCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGa                                              >  1:8485170/1‑140 (MQ=255)
                                                                                            ttttttGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAatat                                          >  2:9685012/1‑140 (MQ=255)
                                                                                                 tGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCg                                     >  1:6579394/1‑140 (MQ=255)
                                                                                                   ggCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAg                                   <  1:4412471/140‑1 (MQ=255)
                                                                                                       cgcgcAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                          >  1:7006958/1‑97 (MQ=255)
                                                                                                       cgcgcAGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGc                               <  1:3927725/140‑1 (MQ=255)
                                                                                                            acgATTTTGACGGAAGAGACGAGTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                          <  1:10704811/138‑1 (MQ=25)
                                                                                                            aGGATTTTGACGGCAGAGACGAATTTGTAGG‑ACGGATCAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGCGCCGCTTTcc                          <  2:953120/140‑1 (MQ=25)
                                                                                                            aGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                          <  1:5785599/140‑1 (MQ=255)
                                                                                                            aGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                          <  2:1215303/140‑1 (MQ=255)
                                                                                                            aGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                          <  2:872934/140‑1 (MQ=255)
                                                                                                            aGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                          <  2:3514310/140‑1 (MQ=255)
                                                                                                            aGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                          <  1:11253782/140‑1 (MQ=255)
                                                                                                            aGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                          <  2:6893539/140‑1 (MQ=255)
                                                                                                            aGGATTTTGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                          <  2:6736324/140‑1 (MQ=255)
                                                                                                                 tttGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGCTGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGata                     <  1:2263814/140‑1 (MQ=255)
                                                                                                                  ttGACGGAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGatat                    <  2:11068387/140‑1 (MQ=255)
                                                                                                                       ggAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAaac               <  1:159607/140‑1 (MQ=255)
                                                                                                                        gAAGAGACGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGAGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAaaca              >  2:3710019/1‑140 (MQ=255)
                                                                                                                            agaCGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCt          <  1:2725018/140‑1 (MQ=255)
                                                                                                                            agaCGATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCt          <  1:2844123/140‑1 (MQ=255)
                                                                                                                                gATTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCAtt      <  1:8581084/140‑1 (MQ=255)
                                                                                                                                 aTTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTg     <  1:644511/140‑1 (MQ=255)
                                                                                                                                 aTTTTGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTg     <  2:5330220/140‑1 (MQ=255)
                                                                                                                                   tttGTAGG‑ACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGcc   >  2:4562654/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                       
GTGTATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAG‑AC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGCCA  >  NZ_CP025268/247154‑247424

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑