BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,855,893	A→G	291 (TAA→TAG) S4G (AGC→GGC)	hypB → hypC →	hydrogenase nickel incorporation protein HypB hydrogenase 3 maturation protein HypC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,855,893	0	A	G	99.8%	1659.5 / ‑5.2	481	291 (TAA→TAG) S4G (AGC→GGC)	hypB hypC	hydrogenase nickel incorporation protein HypB hydrogenase 3 maturation protein HypC
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (272/207); total (273/208)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

Only 100 of 481 total aligned reads displayed.

TCAACTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGCCAGTGGGTACT  >  NZ_CP025268/2855757‑2856029

tcAACTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCg                                                                                                                                       >  2:11346335/1‑140 (MQ=255)

tcAACTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCg                                                                                                                                       >  1:9696395/1‑140 (MQ=255)

tcAACTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCg                                                                                                                                       >  1:9468557/1‑140 (MQ=255)

tcAACTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCg                                                                                                                                       >  1:81669/1‑140 (MQ=255)

   aCTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTc                                                                                                                                    <  1:10377052/140‑1 (MQ=255)

   aCTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTc                                                                                                                                    >  2:1337845/1‑140 (MQ=255)

   aCTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTc                                                                                                                                    <  2:10802383/140‑1 (MQ=255)

    cTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTcc                                                                                                                                   <  2:6782765/140‑1 (MQ=255)

      ttGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCATATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCg                                                                                                                                 >  2:8829539/1‑140 (MQ=255)

       tGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACCGCGATGTGCATAGGCGTTCCCgg                                                                                                                                >  2:7621243/1‑140 (MQ=255)

       tGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCgg                                                                                                                                >  1:622304/1‑140 (MQ=255)

       tGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCgg                                                                                                                                >  2:5600411/1‑140 (MQ=255)

        gACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGc                                                                                                                               >  1:11304658/1‑140 (MQ=255)

        gACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGc                                                                                                                               <  2:622304/140‑1 (MQ=255)

         tcGTTGAAAAGTGCATCGCCTGCGCCCGCGACGTCAATCCAGAACTTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGcc                                                                                                                              <  2:10927890/139‑1 (MQ=255)

         aCGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCCATCCAGACAGTGCAATCATCCTTATATCCGCCACCAGCGGCGACGGGCTGGCCCAGTGGCTGAACTGGCTGGAGACCCAGCGATGTGCATAGGCGGTCCCGGcc                                                                                                                              >  2:2729552/1‑140 (MQ=255)

             tGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGAt                                                                                                                          <  1:2512881/140‑1 (MQ=255)

              gAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATc                                                                                                                         >  2:702586/1‑140 (MQ=255)

              gAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATc                                                                                                                         <  1:781662/140‑1 (MQ=255)

              gAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATc                                                                                                                         >  1:3436276/1‑140 (MQ=255)

                  aGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTCTTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCa                                                                                                                     <  1:526636/140‑1 (MQ=255)

                    tGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCAcc                                                                                                                   >  1:6401324/1‑140 (MQ=255)

                     gCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCa                                                                                                                  >  1:2711822/1‑140 (MQ=255)

                     gCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCa                                                                                                                  >  1:599930/1‑140 (MQ=255)

                     gCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCa                                                                                                                  <  1:9214318/140‑1 (MQ=255)

                      cATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCAt                                                                                                                 >  2:1752453/1‑140 (MQ=255)

                         cGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGa                                                                                                              >  2:1549820/1‑140 (MQ=255)

                              gcgcCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCa                                                                                                         >  2:198645/1‑140 (MQ=255)

                              gcgcCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCa                                                                                                         >  1:2333129/1‑140 (MQ=255)

                              gcgcCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCa                                                                                                         >  2:6676969/1‑140 (MQ=255)

                              gcgcCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCa                                                                                                         >  2:5951139/1‑140 (MQ=255)

                                 cccGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAAcc                                                                                                      <  1:6898690/140‑1 (MQ=255)

                                    gcgAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAgg                                                                                                   <  1:5632088/140‑1 (MQ=255)

                                     cgAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGc                                                                                                  <  1:825782/140‑1 (MQ=255)

                                          tCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAg                                                                                             <  1:396481/140‑1 (MQ=255)

                                                   aaaTTGAAATCATCCTTATTTCCGCCACCCGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCt                                                                                    <  1:132256/140‑1 (MQ=255)

                                                    aaTTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTg                                                                                   >  1:8939030/1‑140 (MQ=255)

                                                      ctGAAATCATCCGTACTTCCGCCCCCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCg                                                                                 <  1:3294806/139‑1 (MQ=255)

                                                                ccTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAgcg                                                                       <  2:4348436/140‑1 (MQ=255)

                                                                    aTTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGAt                                                                   >  1:6759800/1‑140 (MQ=255)

                                                                    aTTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGAt                                                                   >  1:4189237/1‑140 (MQ=255)

                                                                     tttCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATg                                                                  <  2:2248813/140‑1 (MQ=255)

                                                                     tttCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATg                                                                  <  1:9859455/140‑1 (MQ=255)

                                                                        cctccaccaGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGACg                                                               >  2:7593595/4‑140 (MQ=255)

                                                                        ccGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCg                                                               >  2:8695695/1‑140 (MQ=255)

                                                                           ccaccaGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCACGATGTCGAtt                                                            <  2:7147506/140‑1 (MQ=255)

                                                                              ccaGCGGCGAAGGGATTGACCAGTTGCTGATCTGGCTGGAGACACAGCGATGTTCATAGTCGTTCCCGGCCAGTTCCGCACACTTGACGGCAACCAGTCGAAAGTCGACGTATGCGTCATTCAGCGCGATGTCGATTTaa                                                         <  2:9521924/140‑1 (MQ=255)

                                                                              ccaGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTaa                                                         >  2:11219466/1‑140 (MQ=255)

                                                                              ccaGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTaa                                                         <  1:3197312/140‑1 (MQ=255)

                                                                                  cggcgAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGtt                                                     >  1:6561525/1‑140 (MQ=255)

                                                                                   ggcgAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTa                                                    >  2:2400416/1‑140 (MQ=255)

                                                                                            aTGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGc                                           >  2:10429459/1‑140 (MQ=255)

                                                                                             tGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCt                                          <  2:4079451/140‑1 (MQ=255)

                                                                                                aCCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCg                                       <  2:8422179/140‑1 (MQ=255)

                                                                                                    ggggCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGa                                   <  1:8013509/138‑1 (MQ=255)

                                                                                                    gTGGCTGAACTGGCTGGAGACCCAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGa                                   >  2:5147043/1‑140 (MQ=255)

                                                                                                    gTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAGCGGTAGTCGGCAGCTGCGATGa                                   >  1:1994500/1‑140 (MQ=255)

                                                                                                    gTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGa                                   >  2:3299678/1‑140 (MQ=255)

                                                                                                    gTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGa                                   >  1:1656597/1‑140 (MQ=255)

                                                                                                    gTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGa                                   >  1:3015825/1‑140 (MQ=255)

                                                                                                    gTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGa                                   >  2:900496/1‑140 (MQ=255)

                                                                                                     tGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGaa                                  >  1:7076474/1‑140 (MQ=255)

                                                                                                     tGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGaa                                  >  1:4833679/1‑140 (MQ=255)

                                                                                                         tGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACg                              >  2:4459697/1‑140 (MQ=255)

                                                                                                         tGAACTGGCTGGAGACAAAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGTCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGTCAGCAGCGAAGAAATCg                              >  2:4658874/1‑140 (MQ=255)

                                                                                                          gAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCTACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGCTTTCACGTTAGTCGGCAGCTGCGATGAACACgg                             <  2:11399343/140‑1 (MQ=255)

                                                                                                          gAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACgg                             >  2:10205588/1‑140 (MQ=255)

                                                                                                          gAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACgg                             >  2:5066793/1‑140 (MQ=255)

                                                                                                           aaCTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGt                            >  2:7700615/1‑140 (MQ=255)

                                                                                                               ggctggAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAgcg                                                                       >  1:5512362/1‑93 (MQ=255)

                                                                                                               ggctggAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAgcg                                                                       <  2:5512361/93‑1 (MQ=255)

                                                                                                                 ctggAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGCTGAAAACGGTCAGCcg                      >  1:10021816/1‑140 (MQ=255)

                                                                                                                     agaCACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCcgcgcg                  <  2:3919382/140‑1 (MQ=255)

                                                                                                                     agaCACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCcgcgcg                  >  2:477338/1‑140 (MQ=255)

                                                                                                                      gaCACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGt                 <  2:7397189/140‑1 (MQ=255)

                                                                                                                        cacaGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTgg               >  2:5368666/1‑140 (MQ=255)

                                                                                                                         acaGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTTACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTggg              <  2:9657310/140‑1 (MQ=255)

                                                                                                                         acaGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTggg              <  2:6489297/140‑1 (MQ=255)

                                                                                                                         acaGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTggg              <  2:2773618/140‑1 (MQ=255)

                                                                                                                         acaGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATGTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTggg              <  2:797535/140‑1 (MQ=255)

                                                                                                                          caGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGc             >  1:11174060/1‑140 (MQ=255)

                                                                                                                          caGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGc             >  1:1745846/1‑140 (MQ=255)

                                                                                                                          caGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGc             >  1:7646267/1‑140 (MQ=255)

                                                                                                                          caGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGc             >  1:1151012/1‑140 (MQ=255)

                                                                                                                          caGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGc             >  2:6065201/1‑140 (MQ=255)

                                                                                                                          caGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGc             >  2:4516112/1‑140 (MQ=255)

                                                                                                                           aGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGcc            >  1:10810253/1‑140 (MQ=255)

                                                                                                                            gCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCACCCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGCCa           <  2:3512898/140‑1 (MQ=255)

                                                                                                                            gCGAGGTGCATAGGCGTGCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGCCa           <  1:870748/140‑1 (MQ=255)

                                                                                                                              gATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAAGCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGCCAGt         <  1:3891295/140‑1 (MQ=255)

                                                                                                                              gATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGCCAGt         >  2:4945226/1‑140 (MQ=255)

                                                                                                                              gATGTGCATAGGCGGTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGCCAGt         <  2:9384671/140‑1 (MQ=255)

                                                                                                                               aTGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGCCAGTg        >  1:2154250/1‑140 (MQ=255)

                                                                                                                                 gtgCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGCCAGTggg      <  2:7208125/140‑1 (MQ=255)

                                                                                                                                 gtgCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGCCAGTggg      <  2:3815923/140‑1 (MQ=255)

                                                                                                                                 gtgCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGCCAGTggg      <  2:8205152/140‑1 (MQ=255)

                                                                                                                                 gtccATAGGCGTTCCCTGCCAGATCCGCACCATTGACGGCAACCATGCGATAGTCGACGTCTGCGGCATTAAGCGCGATGTCGATTTAACTTTAGTCGGCAGCTGAGATGAAAACGGTCAGCCGCGCGTGGGCCAGTggg      <  2:10579561/137‑1 (MQ=255)

                                                                                                                                  tgCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGACAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGCCAGTGGGt     >  2:3153948/1‑140 (MQ=255)

                                                                                                                                  tgCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGCCAGTGGGt     >  1:7397869/1‑140 (MQ=255)

                                                                                                                                   gCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGCCAGTGGGTa    <  2:295708/140‑1 (MQ=255)

TCAACTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGCCAGTGGGTACT  >  NZ_CP025268/2855757‑2856029

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`