BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	306,029	C→T	V786M (GTG→ATG)	ecpC ←	fimbrial usher EcpC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	306,029	0	C	T	98.4%	2093.6 / NA	569	V786M (GTG→ATG)	ecpC	fimbrial usher EcpC
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (281/279); minor base G (3/6); total (284/285)
Fisher's exact test for biased strand distribution p-value = 5.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 572 total aligned reads displayed.

CGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCACGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCTTCACCTCTGGCTCA  >  NZ_CP025268/305892‑306165

          gATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACaaa                                                                                                                              <  2:1284595/140‑1 (MQ=255)

              agagGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCg                                                                                                                          <  1:10853824/140‑1 (MQ=255)

                  gCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGt                                                                                                                      >  1:2314915/1‑140 (MQ=255)

                    cGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGAGAGTGGGGTCTTTCTTATCCAGGTCCAGGACAAACTCGCCGgtt                                                                                                                    >  2:6378474/1‑140 (MQ=255)

                     gCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTGATTGCCACTGTCGCGAAGTGCGATAGTGGGGTAGTTCGTTTCCAGGTCAGTGACAATCTCGCCGtgtg                                                                                                                   >  2:9150649/1‑137 (MQ=255)

                      cTGCAGACCACATCACCGACCAAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGACACTTCGCAGGTTTTATTGCCACTGAAGCGAAAATCGATAGTGGGGTATTTCTTATCCCTGTCCATGACAAACTCGCCGtggtc                                                                                                                  >  1:9129407/1‑136 (MQ=255)

                        gCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCAt                                                                                                                <  2:6480168/140‑1 (MQ=255)

                        gCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCAt                                                                                                                <  1:1109653/140‑1 (MQ=255)

                        gCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCAt                                                                                                                <  2:3048067/140‑1 (MQ=255)

                          aGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCg                                                                                                              >  2:6745285/1‑140 (MQ=255)

                                caTCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAACATCGATAGTGGGGTATTTCTTATCCCTGTCCATGACAAACTCGCCGTTTTCATCggtgcgg                                                                                                        >  1:1203804/1‑140 (MQ=255)

                                caTCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCggttcgg                                                                                                        >  1:9326595/1‑140 (MQ=255)

                                caTCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCggttcgg                                                                                                        <  1:6900169/140‑1 (MQ=255)

                                 aTCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCggttcggg                                                                                                       >  2:10792257/1‑140 (MQ=255)

                                   cACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCggttcgggtt                                                                                                     >  1:8658861/1‑140 (MQ=255)

                                   cACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCggttcgggtt                                                                                                     >  1:7195699/1‑140 (MQ=255)

                                   cACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCggttcgggtt                                                                                                     <  1:7349471/140‑1 (MQ=255)

                                   cACCGACCCAGACGGCACCGCGCGCCTCGTTGAGTTCCAGAGCCACTTCGCAGGATTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCggttcgggtt                                                                                                     <  1:7601143/140‑1 (MQ=255)

                                     ccGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTGTTCATCggttcgggttcg                                                                                                   >  2:10391864/1‑140 (MQ=255)

                                       gACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGc                                                                                                 <  2:4998996/140‑1 (MQ=255)

                                       gACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGc                                                                                                 <  1:1414200/140‑1 (MQ=255)

                                        aCCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGcc                                                                                                >  1:3943912/1‑140 (MQ=255)

                                        aCCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGcc                                                                                                >  2:5680940/1‑140 (MQ=255)

                                        aCCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGcc                                                                                                >  2:4818307/1‑140 (MQ=255)

                                        aCCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGcc                                                                                                >  2:4374501/1‑140 (MQ=255)

                                        aCCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGcc                                                                                                >  2:11251425/1‑140 (MQ=255)

                                        aCCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGc                                                                                                 <  1:11386072/139‑1 (MQ=255)

                                        aCCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGGTCGGcc                                                                                                >  1:3651262/1‑140 (MQ=255)

                                          ccAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGa                                                                                              >  2:4519480/1‑140 (MQ=255)

                                           cAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGat                                                                                             >  1:1654567/1‑140 (MQ=255)

                                            aGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGata                                                                                            >  2:1358089/1‑140 (MQ=255)

                                            aGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGata                                                                                            <  1:792573/140‑1 (MQ=255)

                                             gACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGatat                                                                                           <  2:9797018/140‑1 (MQ=255)

                                             gACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGatat                                                                                           <  1:10490119/140‑1 (MQ=255)

                                             gACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGatat                                                                                           <  1:1790832/140‑1 (MQ=255)

                                             gACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGatat                                                                                           <  2:881409/140‑1 (MQ=255)

                                             gACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGatat                                                                                           <  1:2588685/140‑1 (MQ=255)

                                             gACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGatat                                                                                           <  2:7501852/140‑1 (MQ=255)

                                             gACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGatat                                                                                           <  1:3346948/140‑1 (MQ=255)

                                             gACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGatat                                                                                           <  1:4163397/140‑1 (MQ=255)

                                             gACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTAGTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGatat                                                                                           <  1:10043318/140‑1 (MQ=255)

                                              aCGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGNTTTCATCGGTTCGGGTTCGGCCGATCTg                                                                                          >  1:6021341/1‑140 (MQ=255)

                                                ggCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAATGCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATggg                                                                                        >  2:2011963/1‑139 (MQ=255)

                                                     cgcgcgCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCTTGACAAACTCGCCGTTTTCATCGGTTCGGGGTCGGCCGATATGGTTGTTa                                                                                   >  1:6565512/1‑140 (MQ=255)

                                                      gcgcgcCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCTTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTaa                                                                                  >  2:7751566/1‑140 (MQ=255)

                                                       cgcgcCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAAt                                                                                 >  2:1443879/1‑140 (MQ=255)

                                                       cgcgcCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAAt                                                                                 >  2:3195995/1‑140 (MQ=255)

                                                       cgcgcCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAAt                                                                                 >  2:3589994/1‑140 (MQ=255)

                                                       cgcgcCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAAt                                                                                 >  2:10567276/1‑140 (MQ=255)

                                                        gcgcCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATc                                                                                <  1:4880286/140‑1 (MQ=255)

                                                              ggTTGAGTTCCAGAGCCTATTCGCAGGTTTTATTGCCACTGTAGAGAATATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCg                                                                          <  2:8661009/140‑1 (MQ=255)

                                                              ggTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCg                                                                          <  2:1971150/140‑1 (MQ=255)

                                                              ggTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTAACCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCg                                                                          <  1:5686322/140‑1 (MQ=255)

                                                              ggTTGAGTTCCAGAGCCACTTCGCAGGATTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCg                                                                          <  2:9580209/140‑1 (MQ=255)

                                                                   aGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGc                                                                     <  2:9068238/140‑1 (MQ=255)

                                                                    gTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGcc                                                                    >  2:3791922/1‑140 (MQ=255)

                                                                      tCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCcag                                                                  <  1:6705114/140‑1 (MQ=255)

                                                                        cAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCcagcg                                                                >  2:8500573/1‑139 (MQ=255)

                                                                        cAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCcagca                                                                >  1:1903955/1‑140 (MQ=255)

                                                                        cAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCcagca                                                                <  2:9372568/140‑1 (MQ=255)

                                                                          gagCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAgt                                                              >  1:7751801/1‑140 (MQ=255)

                                                                          gagCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAgt                                                              >  2:5832104/1‑140 (MQ=255)

                                                                               aCTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGcc                                                         <  1:9415668/140‑1 (MQ=255)

                                                                               aCTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGcc                                                         >  2:6074287/1‑140 (MQ=255)

                                                                                cTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCg                                                        >  1:3812637/1‑140 (MQ=255)

                                                                                    gCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCtt                                                    <  1:3412420/140‑1 (MQ=255)

                                                                                     cAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTc                                                   <  2:7233271/140‑1 (MQ=255)

                                                                                        gTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGc                                                <  1:4003212/140‑1 (MQ=255)

                                                                                        gTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGc                                                <  1:95763/140‑1 (MQ=255)

                                                                                          tttATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCAc                                              >  2:2780887/1‑140 (MQ=255)

                                                                                          tttATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCAc                                              >  1:4338219/1‑140 (MQ=255)

                                                                                              ttGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGAt                                          <  1:10729681/140‑1 (MQ=255)

                                                                                                  cACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGa                                      >  1:5087745/1‑140 (MQ=255)

                                                                                                    cTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCAGCACGGATACGAcc                                    >  1:8075062/1‑140 (MQ=255)

                                                                                                      gTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCgg                                  <  1:274001/140‑1 (MQ=255)

                                                                                                           gAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGGTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACg                             >  1:5250662/1‑140 (MQ=255)

                                                                                                            acaaTCGAGAGGGGGGTATTTCTTATCCCTGTCCATGACAAACTCGCCGTTTTCAGCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGTGTCgg                            >  2:5306679/3‑140 (MQ=255)

                                                                                                              aaTCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGata                                                                                            <  1:8205699/74‑1 (MQ=255)

                                                                                                              aaTCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGata                                                                                            >  2:8205698/1‑74 (MQ=255)

                                                                                                               aTCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTaa                         <  2:229973/140‑1 (MQ=255)

                                                                                                               aTCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTaa                         >  2:8329487/1‑140 (MQ=255)

                                                                                                                 cGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAccc                       >  2:1550834/1‑140 (MQ=255)

                                                                                                                 cGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAAcc                       >  2:4136244/1‑140 (MQ=255)

                                                                                                                 cGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGAGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAAcc                       >  1:3054610/1‑140 (MQ=255)

                                                                                                                  gATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCa                      <  2:10067571/140‑1 (MQ=255)

                                                                                                                   aTAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGCTACGACCGGAGACGGTCACCag                     >  2:10979821/1‑139 (MQ=255)

                                                                                                                   aTAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCAt                     <  2:172633/140‑1 (MQ=255)

                                                                                                                   aTAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCAt                     <  2:7997530/140‑1 (MQ=255)

                                                                                                                    tAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATc                    <  2:5954618/140‑1 (MQ=255)

                                                                                                                        ggggTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCt                <  1:10313816/140‑1 (MQ=255)

                                                                                                                         gggTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCtt               <  1:9802401/140‑1 (MQ=255)

                                                                                                                         gggTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCtt               <  1:9997729/140‑1 (MQ=255)

                                                                                                                              tttCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCTTCACct          >  2:4036415/1‑140 (MQ=255)

                                                                                                                                tCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCTTCACctct        <  1:6208783/140‑1 (MQ=255)

                                                                                                                                 cTTCTCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGGCGATATGGTTGTTAATCCGTGGGGTTGGCAGCAGTGTGGCGTCGTCAGCTCGGAGACGACCGGAGTGGGGCACCAGCTGCTTCTCCTCTg       >  2:5474295/1‑140 (MQ=255)

                                                                                                                                 cTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCTTCACCTCTg       >  2:7003227/1‑140 (MQ=255)

                                                                                                                                  ttATCCATGTCCATGACACACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGGTGTTAATCCGTGCGTTAGCCAGCCGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCTTCACCTCTgg      <  2:2206287/140‑1 (MQ=255)

                                                                                                                                  ttATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCTTCACCTCTgg      <  2:11356838/140‑1 (MQ=255)

                                                                                                                                   tATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCTTCACCTCTGGc     >  1:8443153/1‑140 (MQ=255)

                                                                                                                                    aTCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCTTCACCTCTGGCt    >  2:6238203/1‑140 (MQ=255)

CGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCACGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCTTCACCTCTGGCTCA  >  NZ_CP025268/305892‑306165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`