BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,913,061	T→C	112 (TAA→TAG)	mazF ←	endoribonuclease MazF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,913,061	0	T	C	99.4%	2190.3 / NA	633	112 (TAA→TAG)	mazF	endoribonuclease MazF
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (365/262); minor base A (4/0); total (370/263)
Fisher's exact test for biased strand distribution p-value = 1.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 637 total aligned reads displayed.

ACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCT  >  NZ_CP025268/2912924‑2913197

aCGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTa                                                                                                                                        >  1:4401242/1‑140 (MQ=255)

 cGGGCAGCCCCTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAACATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTAc                                                                                                                                       <  1:10815796/140‑1 (MQ=255)

  gggCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTAcc                                                                                                                                      <  2:5573732/140‑1 (MQ=255)

  gggCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTAcc                                                                                                                                      >  2:10095156/1‑140 (MQ=255)

  gggCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTAcc                                                                                                                                      >  1:7375200/1‑140 (MQ=255)

  gggCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTAcc                                                                                                                                      >  1:1226515/1‑140 (MQ=255)

       gCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATc                                                                                                                                 <  2:5139483/140‑1 (MQ=255)

       gCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATc                                                                                                                                 >  2:10757036/1‑140 (MQ=255)

         cGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAg                                                                                                                               <  1:3813304/140‑1 (MQ=255)

                    tCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCCGTACGTTAAttt                                                                                                                    <  1:7706877/140‑1 (MQ=255)

                     cgcgCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTTGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCATTACGTTAAtttt                                                                                                                   >  1:10218769/1‑140 (MQ=255)

                     cgcgCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCGGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGGTAAtttt                                                                                                                   >  1:2366572/1‑140 (MQ=255)

                      gcgcAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTg                                                                                                                  <  2:6763887/140‑1 (MQ=255)

                      gcgcAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTg                                                                                                                  <  1:7169605/140‑1 (MQ=255)

                       cgcAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCAGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTgg                                                                                                                 >  2:1856610/1‑140 (MQ=255)

                         cAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCt                                                                                                               <  1:9909035/140‑1 (MQ=255)

                               cTGCATCAGAGTGAGCAAACGGTCGATTTGATTCATTGAAGTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCACTCAGTACGTTAATTTTGGCTTTAATg                                                                                                         <  2:11157622/140‑1 (MQ=255)

                               cTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATg                                                                                                         <  1:1565022/140‑1 (MQ=255)

                               cTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATg                                                                                                         <  1:8315297/140‑1 (MQ=255)

                                 gCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAAATGTACTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTATTAACAATACCCAATAAGTACGTTTATTTTGGCTTTAATggt                                                                                                       >  1:60477/1‑138 (MQ=255)

                                  catcatAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGt                                                                                                      <  1:9598482/140‑1 (MQ=255)

                                  cATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGt                                                                                                      >  1:11061382/1‑140 (MQ=255)

                                       taGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTaa                                                                                                 >  2:10478583/1‑140 (MQ=255)

                                        aGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAAt                                                                                                >  1:8472799/1‑140 (MQ=255)

                                           gAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTGAATGAGTTGTAATTcc                                                                                             <  2:1649125/140‑1 (MQ=255)

                                            aGCAAACGGTCGATTTGATTCATTGAATTTTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCct                                                                                            >  1:698219/1‑140 (MQ=255)

                                            aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTcat                                                                                            >  2:10973872/1‑138 (MQ=255)

                                            aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCct                                                                                            >  2:2250243/1‑140 (MQ=255)

                                            aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCct                                                                                            >  2:4742976/1‑140 (MQ=255)

                                            aGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCct                                                                                            >  2:9253438/1‑140 (MQ=255)

                                              cAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCctct                                                                                          >  2:5808497/1‑140 (MQ=255)

                                                 aCGGTCGATTTGATTCATTGAATTTTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGGTTTAATGAGTTGTAATTCCTCTggg                                                                                       >  1:6257021/1‑140 (MQ=255)

                                                 aCGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTggg                                                                                       >  1:2720967/1‑140 (MQ=255)

                                                    gTCGATTTGATTCATTGAGTTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCa                                                                                    >  1:3725950/1‑140 (MQ=255)

                                                    gTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCACTCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTACTCTGGGGCa                                                                                    >  1:2539480/1‑140 (MQ=255)

                                                     tCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCaa                                                                                   <  1:7846771/140‑1 (MQ=255)

                                                                   tGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCACTCAGTACGCTAATTTTGGCTTTAATGAGt                                                                                                      >  2:2429761/1‑107 (MQ=255)

                                                                   tGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTttcttc                                                                     >  2:10623922/1‑140 (MQ=255)

                                                                   agAATTGTCCTGAAAATTGCGTGTCTGTCAGGTGGAAACCTGTGACCCGACTAGAAGAGAGTTAGTAACACTACCCAATCAGTACGTTACTGAAGGCTTTAATCAGTTGTTCTTCCTCTGGGGCAAATGTAGCTttcttc                                                                     >  2:9154740/2‑140 (MQ=255)

                                                                       ttGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTg                                                                 <  2:1452452/140‑1 (MQ=255)

                                                                       ttGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTg                                                                 >  1:4859894/1‑140 (MQ=255)

                                                                          tCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCct                                                                                            >  2:5292630/1‑110 (MQ=255)

                                                                          tCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGctc                                                              <  1:10050077/140‑1 (MQ=255)

                                                                          tCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGctc                                                              >  1:9097295/1‑140 (MQ=255)

                                                                           ccTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGctcc                                                             >  2:206309/1‑140 (MQ=255)

                                                                            cTGAAAATTGCGGGTCTGACAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTAGGTTAATTTTGGCTTTAATGAGTTGTAATACCTCTGGGGCAACTGTTCCTATCTTCGTTGatcct                                                            >  1:5551831/1‑140 (MQ=255)

                                                                                    tGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTCACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCg                                                    <  2:8082593/140‑1 (MQ=255)

                                                                                    tGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGt                                                                   <  1:11346320/125‑1 (MQ=255)

                                                                                    tGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGt                                                                   >  2:11346319/1‑125 (MQ=255)

                                                                                    tGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCg                                                    >  1:4128315/1‑140 (MQ=255)

                                                                                    tGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGAGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCg                                                    >  2:7116692/1‑140 (MQ=255)

                                                                                    tGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGGTAGTAACACTACCCAATCAGTACGTTACTTTTGGCTTTAATGAGGTGTAATTGCGCTGGGGCAACTGTTCGTTTCGTCTTTGCTCCTCGTGCCCg                                                    >  2:7770568/1‑140 (MQ=255)

                                                                                         gTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAAGTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAgg                                               <  2:2938128/140‑1 (MQ=255)

                                                                                            tGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCTATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGATCCTCTTGCCCGCCAGGCGa                                            <  2:823047/140‑1 (MQ=255)

                                                                                            tGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGa                                            <  2:2684076/140‑1 (MQ=255)

                                                                                                     gAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTa                                   >  1:3231025/1‑140 (MQ=255)

                                                                                                      aaaCCTGTGACCAGAATAGAAGTGAGTTAGTAAAACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTAc                                  <  1:2955729/140‑1 (MQ=255)

                                                                                                        aCCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTACGTCCTCTGGGGCAACTGTTCCTGTCTTCGTTGCTGCTCTTGCCCGCCAGGCGATACTTTTTACCt                                >  2:7448388/1‑140 (MQ=255)

                                                                                                        aCCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCt                                >  1:1387039/1‑140 (MQ=255)

                                                                                                        aCCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCt                                >  2:7568086/1‑140 (MQ=255)

                                                                                                        aCCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCt                                >  1:708208/1‑140 (MQ=255)

                                                                                                        aCCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCGCTGGGGCAACTGTTCCTTTCTTCGTTGCTACTCTTGCCCGCCAGGCGCTACTTTTTACCt                                >  2:1253098/1‑140 (MQ=255)

                                                                                                          cTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGa                              <  1:180510/140‑1 (MQ=255)

                                                                                                          cTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGa                              <  1:9253446/140‑1 (MQ=255)

                                                                                                          cTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCGTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGa                              <  2:4867649/140‑1 (MQ=255)

                                                                                                           tgtgACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGAt                             <  2:7006628/140‑1 (MQ=255)

                                                                                                            gtgACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGAAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATc                            >  1:10296747/1‑140 (MQ=255)

                                                                                                            gggACCCGAATAGACGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTGTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTATTTACCTGATc                            >  2:3656478/3‑140 (MQ=255)

                                                                                                             tgACCAGAATTGAAGTGAGATAGTAACACTACCCAATCAGTACGTTATTTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCa                           <  1:3420034/140‑1 (MQ=255)

                                                                                                             tgACCAGAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTttctt                                                                      <  1:2841611/97‑1 (MQ=255)

                                                                                                                 cAGAATAGAAGTGAGTTATTAACACTACCCAATCAGTACGTTACTTTTGTCTTTAATGAGTTGTATTTCCTCTGGGGGAACTGTTACTTTCTTCGTTGCTCCTCTTGCACGCCAGTCTATACTTATTAAATGATCAGCTa                       >  2:6303531/1‑140 (MQ=255)

                                                                                                                   gAATAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAAc                     >  1:9764266/1‑140 (MQ=255)

                                                                                                                    aaTAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCTACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACg                    >  2:8807997/1‑140 (MQ=255)

                                                                                                                     aTAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGc                   <  2:9364427/140‑1 (MQ=255)

                                                                                                                     aTAGAAGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGc                   <  1:206309/140‑1 (MQ=255)

                                                                                                                         aaGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACg               <  2:3526818/140‑1 (MQ=255)

                                                                                                                         aaGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACg               <  2:3454891/140‑1 (MQ=255)

                                                                                                                          aGTGAGTTCTTTACACTACCCTTTCAGTTCGTTTTTTTTGGATTTAATGTGTTGTAATTACTCTGTGGCAACTGTTCCTTTCTTCGTTGCTCGTCTTGCCCGCCAGGCGATACTTTTTAACTGATCAGCTAACGCTACGc              <  2:5994929/140‑1 (MQ=255)

                                                                                                                          aGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGc              <  2:6476446/140‑1 (MQ=255)

                                                                                                                          aGTGAGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTGACCTGATCAGCTAACGCTACGc              <  1:882732/140‑1 (MQ=255)

                                                                                                                              aGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCaa                                                                                   <  1:3097585/67‑1 (MQ=255)

                                                                                                                              aGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCaa                                                                                   >  2:4218639/1‑67 (MQ=255)

                                                                                                                              aGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCaa                                                                                   >  2:3097584/1‑67 (MQ=255)

                                                                                                                              aGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATc          <  1:804543/140‑1 (MQ=255)

                                                                                                                              aGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATc          >  2:4983945/1‑140 (MQ=255)

                                                                                                                              aGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATc          >  2:4417249/1‑140 (MQ=255)

                                                                                                                              aGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATc          >  1:5888135/1‑140 (MQ=255)

                                                                                                                              aGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATc          >  1:5340131/1‑140 (MQ=255)

                                                                                                                              aGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATc          >  1:10658022/1‑140 (MQ=255)

                                                                                                                              aGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATc          >  1:10480191/1‑140 (MQ=255)

                                                                                                                              aGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACATGATCAGCTAACGCTACGCCATc          >  2:9731736/1‑140 (MQ=255)

                                                                                                                              aGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCATTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATc          >  1:5001179/1‑140 (MQ=255)

                                                                                                                              aGTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTGTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATc          >  1:6546039/1‑140 (MQ=255)

                                                                                                                              aGTTAGTAACACGACCCAATCAGTACGTTAAATTGGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATc          <  2:10294075/140‑1 (MQ=255)

                                                                                                                               gTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCa         >  1:1664490/1‑140 (MQ=255)

                                                                                                                               gTTAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCa         <  2:4128314/140‑1 (MQ=255)

                                                                                                                                 tAGTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACg       <  1:4629831/140‑1 (MQ=255)

                                                                                                                                   gTAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCATCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATAATTTTTACCTGATCAGCTAACGCTACGCCATCACGtt     >  2:7968916/1‑140 (MQ=255)

                                                                                                                                    tAACACTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTc    <  2:8329005/140‑1 (MQ=255)

                                                                                                                                      acacTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCt  >  2:8524225/1‑140 (MQ=255)

ACGGGCAGCCGTTTTCCGGATCGCGCAGGCGCTGCATAATAGTGAGCAAACGGTCGATTTGATTCATTGAATTGTCCTGAAAATTGCGGGTCTGTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACATTACCCAATCAGTACGTTAATTTTGGCTTTAATGAGTTGTAATTCCTCTGGGGCAACTGTTCCTTTCTTCGTTGCTCCTCTTGCCCGCCAGGCGATACTTTTTACCTGATCAGCTAACGCTACGCCATCACGTTCCT  >  NZ_CP025268/2912924‑2913197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`