Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 2,940,107 G→A G112D (GGC→GAC)  fucK → L‑fuculokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252682,940,1070GA99.6% 2687.2 / ‑6.5 709G112D (GGC→GAC) fucKL‑fuculokinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (306/400);  minor base T (1/2);  total (307/402)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 709 total aligned reads displayed.

TAGTGAACTGACTGAATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGCCTGG  >  NZ_CP025268/2939970‑2940243
                                                                                                                                         |                                                                                                                                        
  gTGAACTGACTGAATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGATCTGGTAGATAAGCAAGGCAATCTGCTCTCTCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGGTATGGACact                                                                                                                                      >  1:5418414/1‑138 (MQ=255)
     aaCTGACTGAATTCCACATCCGCGGTATCGCCGTCACCACCGTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATAtt                                                                                                                                   >  2:10957005/1‑140 (MQ=255)
     aaCTGACTGAATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATAtt                                                                                                                                   >  1:1975416/1‑140 (MQ=255)
      actgactgAATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTg                                                                                                                                  <  2:1518831/140‑1 (MQ=255)
        tgactgaATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGaa                                                                                                                                <  2:4126760/140‑1 (MQ=255)
        tgactgaATGACACATCCGCTGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGaa                                                                                                                                <  2:7771829/140‑1 (MQ=255)
          actgaATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCCGCGGTTATGGACAAGATTGAACg                                                                                                                              >  2:8440377/1‑140 (MQ=255)
          actgaATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAAct                                                                                                                              >  2:10210706/1‑139 (MQ=255)
          actgaATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACg                                                                                                                              >  1:4226504/1‑140 (MQ=255)
             gaATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGtt                                                                                                                           <  1:8737215/140‑1 (MQ=255)
             gaATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGtt                                                                                                                           <  2:1264193/140‑1 (MQ=255)
             gaATGCCACATCCGCGGGATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGtt                                                                                                                           <  2:6232167/140‑1 (MQ=255)
               aTGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTaa                                                                                                                         >  1:6951042/1‑140 (MQ=255)
                tGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAAt                                                                                                                        <  2:11421444/140‑1 (MQ=255)
                    acaTCCGCGGTATCGCCGTCACCACCGTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTCTTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTcc                                                                                                                    >  1:5798784/1‑140 (MQ=255)
                      aTCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGc                                                                                                                  <  2:275992/140‑1 (MQ=255)
                          gcgGTATCGCCGTCACCACCATTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAg                                                                                                              <  1:3179040/140‑1 (MQ=255)
                                tCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTg                                                                                                        >  1:10042865/1‑140 (MQ=255)
                                tCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTAGTAGCTGGAAATGTCCGCGAACAGCAGCGGGTAGGGACAATAGTGAACGGTTAATCTCCGCACCGCGGTTg                                                                                                        >  1:7927072/1‑140 (MQ=255)
                                 cGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGc                                                                                                       >  1:2965416/1‑140 (MQ=255)
                                  gCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCa                                                                                                      <  2:5754954/140‑1 (MQ=255)
                                    cGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAgg                                                                                                    >  2:887838/1‑140 (MQ=255)
                                          caccTTTGGTGTGGATGGCGCTCTGGTAAATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTAttt                                                                                              >  2:8058398/1‑140 (MQ=255)
                                              tttGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGATATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTgg                                                                                          >  2:1953097/1‑140 (MQ=255)
                                                tGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCg                                                                                        >  1:1694550/1‑140 (MQ=255)
                                                    gtgGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGACCAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCgg                                                                                    >  2:9705599/1‑140 (MQ=255)
                                                    gtgGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCgg                                                                                    <  1:7580143/140‑1 (MQ=255)
                                                    gtgGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCgg                                                                                    >  2:7470337/1‑140 (MQ=255)
                                                     tgGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGa                                                                                   >  1:3629601/1‑140 (MQ=255)
                                                       gATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTCTTTCTGGCGTCGGAGc                                                                                 >  2:7609408/1‑140 (MQ=255)
                                                          ggCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCtt                                                                              <  2:11200129/140‑1 (MQ=255)
                                                          ggCGCTCTCGTAGATAAGCAAGGCACTAAGCTCTAACCGATTATTAGCGGGACAGGTCCGCGCACAGCAGCCGTTCTGGACAATATTGACCGGTGAATCTCCGCACAGCGGTTGCAGTCTCTATCGGGCGTCGGAGcggt                                                                              >  2:6493631/1‑137 (MQ=255)
                                                            cgcTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTa                                                                            >  2:11124792/1‑140 (MQ=255)
                                                              ctctGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGt                                                                          <  2:2888690/140‑1 (MQ=255)
                                                                   gTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCATCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCaa                                                                     <  1:8181947/140‑1 (MQ=255)
                                                                    tAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAAt                                                                    <  2:5134988/140‑1 (MQ=255)
                                                                     aGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATa                                                                   <  2:6358340/140‑1 (MQ=255)
                                                                     aGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATa                                                                   <  1:1263601/140‑1 (MQ=255)
                                                                     aGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATa                                                                   <  2:9012077/140‑1 (MQ=255)
                                                                     aGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATa                                                                   <  2:3734162/140‑1 (MQ=255)
                                                                     aGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATa                                                                   <  2:470398/140‑1 (MQ=255)
                                                                     aGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATa                                                                   <  1:248578/140‑1 (MQ=255)
                                                                     aGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATa                                                                   <  2:692240/140‑1 (MQ=255)
                                                                     aGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATa                                                                   <  2:5312069/140‑1 (MQ=255)
                                                                     aGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATa                                                                   <  2:600660/140‑1 (MQ=255)
                                                                     aGATAAGCAACGCAATCTGCTCAATCCGATTAATAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATa                                                                   <  2:5878348/140‑1 (MQ=255)
                                                                       aTAAGCAAGGCAATCTGCTCTATCCGATTATTCGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACg                                                                 <  1:6730804/140‑1 (MQ=255)
                                                                       aTAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACg                                                                 <  1:10251493/140‑1 (MQ=255)
                                                                       aTAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACg                                                                 <  1:2783678/140‑1 (MQ=255)
                                                                       aTAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACg                                                                 <  1:439118/140‑1 (MQ=255)
                                                                       aTAAGCAAGGCAATCTGCTCTATCCGATTATTAGATGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACg                                                                 <  1:8333329/140‑1 (MQ=255)
                                                                       aTAAGCAAGGCAATCTGCTCGATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACg                                                                 <  1:10692171/140‑1 (MQ=255)
                                                                         aaGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGtt                                                               <  1:4884557/140‑1 (MQ=255)
                                                                           gCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTtat                                                             >  1:5157799/1‑140 (MQ=255)
                                                                           gCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTtat                                                             >  2:3014217/1‑140 (MQ=255)
                                                                           gCAAGGCAATCTGCTCTATCCGATTATGAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTtat                                                             <  1:6353620/140‑1 (MQ=255)
                                                                            cAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACTGAGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTtata                                                            <  2:43401/140‑1 (MQ=255)
                                                                             aaGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTCTGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTGTAGATTCAATACGTtttat                                                           >  2:6772164/1‑140 (MQ=255)
                                                                             aaGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATAATGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTCTTTCTGGCGTCGGAGCCTTTAGTTGCAATACGTtatat                                                           >  1:8525667/1‑140 (MQ=255)
                                                                              aGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTtatata                                                          >  2:2491312/1‑140 (MQ=255)
                                                                              aGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTtatata                                                          >  2:5545513/1‑140 (MQ=255)
                                                                                    tCTGCTCTNTCCGAGTATTAGCTGGAAATGTCCGCGAACAGCAGGGGTTATGGACAATNTTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTgg                                                    >  1:6067103/1‑140 (MQ=255)
                                                                                    tCTGCTCTATCCTATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTgg                                                    >  2:1072501/1‑140 (MQ=255)
                                                                                    tCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACg                                                                 <  1:4963576/127‑1 (MQ=255)
                                                                                    tCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTgg                                                    >  2:4880884/1‑140 (MQ=255)
                                                                                     cTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGgt                                                   <  2:3913698/140‑1 (MQ=255)
                                                                                      tGCTCTATCCGATTATTAGCTGGAAATGGCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGgtg                                                  <  2:2367673/140‑1 (MQ=255)
                                                                                       gCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGgtgt                                                 <  1:3931160/140‑1 (MQ=255)
                                                                                         tctATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTgg                                               >  1:9755316/1‑140 (MQ=255)
                                                                                          ctATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGt                                              <  1:7178117/140‑1 (MQ=255)
                                                                                           tATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGtt                                             >  1:10545326/1‑140 (MQ=255)
                                                                                           tATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGtt                                             <  2:10598461/140‑1 (MQ=255)
                                                                                             tCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTga                                           >  2:3364177/1‑140 (MQ=255)
                                                                                             tCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTga                                           >  1:11183979/1‑140 (MQ=255)
                                                                                                gATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTACTCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTgaaag                                        >  2:7274994/1‑140 (MQ=255)
                                                                                                  ttattaGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTGCAATACGTTCTATAAGTTGGTGTGGTTgaaagaa                                      >  1:7707286/1‑140 (MQ=255)
                                                                                                    attaGCTGGACATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGATAATCTCCGCACAGCGGTTGCAGGCTAGTTCTGGCGTCGGAGCCTATAGTTTCACTACGTTATATAAGTTGGTGTGGTTGAAAGaaaa                                    <  1:10931135/140‑1 (MQ=255)
                                                                                                    attaGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGaaaa                                    <  1:3707754/140‑1 (MQ=255)
                                                                                                       aGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTCTCTAAGTTGGTGTGGTTGAAAGAAAatca                                 >  1:9121313/1‑140 (MQ=255)
                                                                                                       aGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAatca                                 >  1:11000299/1‑140 (MQ=255)
                                                                                                       aGCTGGAAATGTCCGCGAACAGCAGCGGATATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAACatca                                 >  2:10483893/1‑140 (MQ=255)
                                                                                                         cTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGATGGTGTGGTTGAAAGAAAatcatc                               <  1:9503011/140‑1 (MQ=255)
                                                                                                          tGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATcc                              <  1:5658135/140‑1 (MQ=255)
                                                                                                            gAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCcac                            <  2:1714339/140‑1 (MQ=255)
                                                                                                            gAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCcac                            <  1:11417351/140‑1 (MQ=255)
                                                                                                            gAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCcac                            <  2:6968227/140‑1 (MQ=255)
                                                                                                            gAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCcac                            <  1:2275232/140‑1 (MQ=255)
                                                                                                            caaaTGTCCGCGAACAGCAGCGGTTATGGACAAAATGCCACGGATACTCTCCGCACAGCGGGAGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATCTAAGTTGGTGTGGGTGAAAGAAAATCATCcac                            <  2:8613110/139‑1 (MQ=255)
                                                                                                              acggcccGCGACCAGCCGCGGGTATGGACAAAATTGAACGGTTAAACTCCGCAAACCGGTTGCAGGCTAGTTCTGGCGTCGTAGCCTTCAGTATCAAAACGATATATAAGATGGTGTGGTTGAAAGAAAATCATCCCCaa                          <  2:10336958/135‑1 (MQ=255)
                                                                                                                  tCCTCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGAGGTTGCAGGCTATTTCTGGCGTCGGAGACTTTAGTTTCAATACGTTATATAAGATGGTGTGGTTGAAAGAAAATAATCCACAActcc                      >  2:4551659/1‑138 (MQ=255)
                                                                                                                  tCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAActgc                      >  2:10040769/1‑140 (MQ=255)
                                                                                                                  tCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAActgc                      >  2:928693/1‑140 (MQ=255)
                                                                                                                   ccGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAActgct                     >  2:9507514/1‑140 (MQ=255)
                                                                                                                    cgcgAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACCActgctg                    >  1:1480074/1‑140 (MQ=255)
                                                                                                                            gcagcGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAAcgcgc            <  2:3189385/140‑1 (MQ=255)
                                                                                                                             cagcGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAAcgcgcg           <  1:7359472/140‑1 (MQ=255)
                                                                                                                                 ggTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACg       >  1:3271162/1‑140 (MQ=255)
                                                                                                                                   ttATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGcc     <  1:652222/140‑1 (MQ=255)
                                                                                                                                    tATGGACAATATTGAACGGTTCATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTGTCAATACGTTATAGAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGCCt    <  2:8220915/140‑1 (MQ=255)
                                                                                                                                      tGGACAATATTGAACGGTTAATCTCCTCACAGCGGTTGCAGTCTATTTCTGGCGTCGGATACTTTAGTTTCAATACTTTTTATAAGTTGTTGTTGTTGAATGAAAATCATCTACAACTGCTGTAACGCGCGCACGCCTgg  <  2:362193/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                        
TAGTGAACTGACTGAATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGCCTGG  >  NZ_CP025268/2939970‑2940243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑