BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,137,127	A→G	N231S (AAT→AGT)	yghT →	dTMP kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,137,127	0	A	G	99.5%	1928.2 / NA	555	N231S (AAT→AGT)	yghT	dTMP kinase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (270/282); minor base T (2/1); total (272/283)
Fisher's exact test for biased strand distribution p-value = 6.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 555 total aligned reads displayed.

AAGATTGCCATAACGCCACAGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑G‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGCCATCAGGATGCTG  >  NZ_CP025268/3136991‑3137263

   aTTGCCATAACGCCACAGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCaaa                                                                                                                                     <  1:8628099/140‑1 (MQ=255)

   aTTGCCATAACGCCACAGTTAACC‑TTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCaaaa                                                                                                                                    <  2:3005501/140‑1 (MQ=255)

    ttGCCATAACGCCACAGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCaaaa                                                                                                                                    >  1:10259517/1‑140 (MQ=255)

    ttGCCATAACGCCACAGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCaaaa                                                                                                                                    >  2:11033394/1‑140 (MQ=255)

           caCGCCACAGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGaaa                                                                                                                             <  1:8024587/139‑1 (MQ=255)

            aCGCCACAGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAAt                                                                                                                            >  1:593489/1‑140 (MQ=255)

             cGCCACAGTTAACCTGTGGTGGTGCACAACTGGGTGATATCGATGCCAATCAGCCACTGGAACAGGTGTGGGTTGATCCAGAAAAAGCGATGACGGATTTTCTGCCCGCGC‑GTGGTTATCCCTAGTCAAAAATGGAAATg                                                                                                                           >  2:6722407/1‑140 (MQ=255)

                 acaGTTAACCTTTGGTGGTGCACATCTGGTTGATATCGATGCCAATCAGCCAGTGGATCAGGTGTTTGTTGATGCAGAAACAGCGATTACGGATTTTATGACCGCGC‑GTGGTTTTAACTAGTCTAAATTGGAAATGCCCg                                                                                                                       <  2:9363279/140‑1 (MQ=255)

                 acaGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCg                                                                                                                       <  1:1242661/140‑1 (MQ=255)

                 acaGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAAGGCCCg                                                                                                                       <  1:6684631/140‑1 (MQ=255)

                 acaGTTAAACTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGTTTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCg                                                                                                                       <  2:1944017/140‑1 (MQ=255)

                   aGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGAt                                                                                                                     <  2:5994388/140‑1 (MQ=255)

                     ttAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCg                                                                                                                   >  1:6446826/1‑140 (MQ=255)

                      tAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGACAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGc                                                                                                                  <  2:1467371/140‑1 (MQ=255)

                       aaCCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTCGTCAAAAATGGAAATGCCCGATCGcc                                                                                                                 >  1:180234/1‑140 (MQ=255)

                        aCCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAATGCGATTACGGATTTTATGAGCGCGC‑GTGGGTATCACTCGGCCAAATTGGTAATGCCCGATCGccg                                                                                                                >  2:6297771/1‑139 (MQ=255)

                        aCCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCa                                                                                                                >  2:4074894/1‑140 (MQ=255)

                            ttGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACCGGTGTTGGTGGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCCATAATGGAAATGCCCGATCGCCAGGAc                                                                                                            >  2:6939837/1‑140 (MQ=255)

                            ttGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGAc                                                                                                            <  1:9338474/140‑1 (MQ=255)

                            ttGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGAc                                                                                                            <  2:5736739/140‑1 (MQ=255)

                             tggtggTGCTCAACTGGTTGATATCGATGCAAATCAGCGACTGGTACTGGTGTTGGTTGTTGCTGTATAAGCGATTACGGATGTGATGAGCGCGC‑GTGGTTAGCACGAGTCACTCTGGGAAATGCCCGATCGACAGGAcg                                                                                                           >  2:7598542/1‑139 (MQ=255)

                               gtggtgCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCgg                                                                                                         >  1:1671700/1‑140 (MQ=255)

                               gtggtgCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCgg                                                                                                         >  2:334866/1‑140 (MQ=255)

                               gtggtgCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCgg                                                                                                         >  1:9085695/1‑140 (MQ=255)

                                tggtgCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCggg                                                                                                        <  1:616229/140‑1 (MQ=255)

                                    tcacaACTGGTTGATATCGTTGCCATTCAGCCTCTGTTACAGGTGTTGGTTGATGCAGAAAAAGCGTTTAAGTATTTTATGACCGCTC‑GTGGTTATCACTAGTCAAATATGGAAATGCCCGATCGCCAGGACCGGTCAtt                                                                                                    <  1:89504/139‑1 (MQ=255)

                                    gCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCAtt                                                                                                    >  2:10132588/1‑140 (MQ=255)

                                         aCTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAg                                                                                               >  1:2938583/1‑140 (MQ=255)

                                          cTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAgg                                                                                              <  2:2154592/140‑1 (MQ=255)

                                             gTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCg                                                                                                                       >  2:4159098/1‑112 (MQ=255)

                                             gTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAg                                                                                           >  1:10598847/1‑140 (MQ=255)

                                             gTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAg                                                                                           >  2:10848816/1‑140 (MQ=255)

                                               tGATATCGATGCCAATCTGCCACTGGAACTGGTGTGGGTTGATGCAGAAAAAGCGATGACGGATTTTATGACCGCGC‑GTGGGTCTCACTAGTCCAAAATGGAAGTGCCCGAGCGCCAGGACCGGGCAGTGTCAGGCAGGt                                                                                         >  2:9108438/1‑140 (MQ=255)

                                                gATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGTAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGtt                                                                                        <  1:4078475/140‑1 (MQ=255)

                                                  tatCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTaa                                                                                      >  2:8931868/1‑140 (MQ=255)

                                                  tatCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTaa                                                                                      >  1:11048931/1‑140 (MQ=255)

                                                    tCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAAt                                                                                    >  1:4537871/1‑140 (MQ=255)

                                                    tCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGAGTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTaatt                                                                                    >  2:10974164/1‑138 (MQ=255)

                                                     cGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATc                                                                                   <  2:320712/140‑1 (MQ=255)

                                                     cGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATc                                                                                   <  1:7746145/140‑1 (MQ=255)

                                                       aTGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCaa                                                                                 >  2:9716319/1‑140 (MQ=255)

                                                       aTGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCaa                                                                                 >  1:5026297/1‑140 (MQ=255)

                                                       aTGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCaa                                                                                 >  2:7912119/1‑140 (MQ=255)

                                                       aTGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCaa                                                                                 >  1:10513289/1‑140 (MQ=255)

                                                        tGCCAATCAGCCACTGGCACAGGTGTTGGTTGATGCAGAAACAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAACTCAAc                                                                                <  2:4441710/140‑1 (MQ=255)

                                                          cctaTCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAACAGCGATTACGGATTTTAGGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAAGGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTg                                                                              <  2:10306706/137‑1 (MQ=255)

                                                          ccAATCAGCCTCTGGAACAGGTGTTGGTTGATGCAGAAACAGCGATTACGGATTTTATGACCGCGCTT‑GGTTATCACTAGTCAAATATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTg                                                                              <  1:7476686/140‑1 (MQ=255)

                                                          ccAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTg                                                                              <  1:5497663/140‑1 (MQ=255)

                                                          ccAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGAGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTg                                                                              <  2:5345288/140‑1 (MQ=255)

                                                           cAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGc                                                                             >  2:11417812/1‑140 (MQ=255)

                                                               cAGCCACTGGCACAGGTGTTGGTTGATGCAGAAAAAGAGATTACGGCTTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                                         <  2:7773976/140‑1 (MQ=255)

                                                               cAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                                         <  2:4831380/140‑1 (MQ=255)

                                                               cAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                                         <  2:7387740/140‑1 (MQ=255)

                                                               cAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                                         <  1:10176090/140‑1 (MQ=255)

                                                               cAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                                         <  1:11400290/140‑1 (MQ=255)

                                                               cAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                                         <  1:347561/140‑1 (MQ=255)

                                                               cAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                                         <  1:7234223/140‑1 (MQ=255)

                                                               cAGACACTTGAACATGTGTTGGTTGATGCAGAAAAAGCGATTACGTATTTTATGACCGCGC‑GTGGTTATCACGAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGTGCATTTTCAGGAAGGTTAAATCAACTGctttg                                                                         <  2:6506451/140‑5 (MQ=255)

                                                                  ccACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTa                                                                      <  1:4822989/140‑1 (MQ=255)

                                                                    aCTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAACATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATc                                                                    <  1:3198202/140‑1 (MQ=255)

                                                                    aCTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTg                                                                              <  1:10007614/130‑1 (MQ=255)

                                                                    aCTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTg                                                                              <  1:2423005/130‑1 (MQ=255)

                                                                    aCTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATc                                                                    >  1:8554088/1‑140 (MQ=255)

                                                                      tGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCa                                                                  >  2:10665884/1‑140 (MQ=255)

                                                                         aaCAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCACCTGCAATGCTATCCAGTa                                                               <  2:2557679/140‑1 (MQ=255)

                                                                         aaCAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTa                                                               <  1:3610272/140‑1 (MQ=255)

                                                                           ccggTGTTGGTTGATGCAGAAAAAGCTATGACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACa                                                             <  2:8210898/138‑1 (MQ=255)

                                                                              gtgtTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGcc                                                          >  2:8380512/1‑140 (MQ=255)

                                                                                  tggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCccacc                                                      >  2:2346376/1‑140 (MQ=255)

                                                                                  tggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCccacc                                                      >  2:5029564/1‑140 (MQ=255)

                                                                                  tggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCccacc                                                      >  2:3864633/1‑140 (MQ=255)

                                                                                  tggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGGTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTGTCAGGAAGGTGAAATCAACTGCAATGCTATCCAGTACAGCccacc                                                      >  1:8225975/1‑140 (MQ=255)

                                                                                   ggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCccacca                                                     >  1:8450971/1‑140 (MQ=255)

                                                                                      tgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCagaa                                                  <  2:8099867/140‑1 (MQ=255)

                                                                                        aTGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCagaaag                                                >  1:10314763/1‑140 (MQ=255)

                                                                                        aTGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCagaaag                                                >  2:617636/1‑140 (MQ=255)

                                                                                        aTGCAGAAAAAGCGATTACGGATATTATGACCGCGC‑GTGGTTATCACTAGTCACAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCagaaag                                                >  1:11228050/1‑140 (MQ=255)

                                                                                         tGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATc                                                                                   <  2:2719196/104‑1 (MQ=255)

                                                                                                   gCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTgccg                                     >  2:4629342/1‑140 (MQ=255)

                                                                                                     gATTACGGATTTTATGACCGCGC‑GTGGTTATCACTCGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTgccgcc                                   <  1:10883379/140‑1 (MQ=255)

                                                                                                      aTTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAAGGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGccgccg                                  <  1:6790960/140‑1 (MQ=255)

                                                                                                        tACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTc                                                                                                                                        <  1:9549331/36‑1 (MQ=255)

                                                                                                         aCGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAACAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGATCTCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGgta                               <  2:713569/140‑1 (MQ=255)

                                                                                                         aCGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGgta                               <  2:11183017/140‑1 (MQ=255)

                                                                                                          cGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAACATGGAAATGCCCGAGCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGgtaa                              <  1:9847104/140‑1 (MQ=255)

                                                                                                          cGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAACTGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGgtaa                              <  1:1459300/140‑1 (MQ=255)

                                                                                                          cGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGgtaa                              <  1:7654585/140‑1 (MQ=255)

                                                                                                                 tATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTATCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAAt                       >  2:1620448/1‑140 (MQ=255)

                                                                                                                      ccGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTCCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCa                  <  1:5280131/140‑1 (MQ=255)

                                                                                                                       cgcgc‑gtGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCAc                 >  1:3807523/1‑140 (MQ=255)

                                                                                                                          cgcgtggTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTCCAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAATTACCCACGc               >  1:7978452/6‑140 (MQ=255)

                                                                                                                          cgcgtggTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGc               >  1:2033940/6‑140 (MQ=255)

                                                                                                                          cgcgtggTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGc               >  2:1364776/6‑140 (MQ=255)

                                                                                                                          cgcgtggTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGc               >  1:4210204/6‑140 (MQ=255)

                                                                                                                          cgcgtggTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGc               >  1:5185753/6‑140 (MQ=255)

                                                                                                                          cgcgtggTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCAGGTTCAGGAAGGGGAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGc               >  2:3758654/6‑140 (MQ=255)

                                                                                                                            cgtggTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGCCa             >  2:8631329/4‑140 (MQ=255)

                                                                                                                             gtggTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGCCAt            <  1:2582369/138‑1 (MQ=255)

                                                                                                                             gtggTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGCCAt            <  1:7076586/138‑1 (MQ=255)

                                                                                                                             gtggTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAACTAAATACCCACGCCAt            <  2:3529401/138‑1 (MQ=255)

AAGATTGCCATAACGCCACAGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑G‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGCCATCAGGATGCTG  >  NZ_CP025268/3136991‑3137263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`