BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,752,014	A→G	287 (TAA→TAG) K3E (AAG→GAG)	sgbU → araD →	L‑ribulose‑5‑phosphate 3‑epimerase L‑ribulose‑5‑phosphate 4‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,752,014	0	A	G	99.8%	1760.6 / NA	506	287 (TAA→TAG) K3E (AAG→GAG)	sgbU araD	L‑ribulose‑5‑phosphate 3‑epimerase L‑ribulose‑5‑phosphate 4‑epimerase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (281/223); minor base T (1/0); total (282/224)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 506 total aligned reads displayed.

CGCTGCATAAACTGAACTATCGCGGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCTTCCGGCGTCGAGTA  >  NZ_CP025268/3751878‑3752150

  cTGCATAAACTGAACTATCGCGGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCac                                                                                                                                     >  1:1129665/1‑139 (MQ=255)

  cTGCATAAACTGAACTATCGCGGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCaa                                                                                                                                     <  1:3171773/140‑1 (MQ=255)

  cTGCATAAACTGAACTATCGCGGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCaa                                                                                                                                     >  1:2778566/1‑140 (MQ=255)

  cTGCATAAACTGAACTATCGCGGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATCGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCaa                                                                                                                                     <  1:8405581/140‑1 (MQ=255)

   tGCATAAACTGAACTATCGCGGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAAc                                                                                                                                    >  1:81751/1‑140 (MQ=255)

   tGCATAAACTGAACTATCGCGGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAAc                                                                                                                                    >  1:9557674/1‑140 (MQ=255)

   tGCATAAACTGAACTATCGCGGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAAc                                                                                                                                    >  2:10301528/1‑140 (MQ=255)

                  aTCGCGGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGc                                                                                                                     >  2:5515051/1‑140 (MQ=255)

                    cgcgGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTg                                                                                                                   >  1:211438/1‑140 (MQ=255)

                     gcgGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTgg                                                                                                                  <  1:2268924/140‑1 (MQ=255)

                      cgGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTggc                                                                                                                 >  2:8814012/1‑140 (MQ=255)

                         ttCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTggcggc                                                                                                              <  1:9143007/140‑1 (MQ=255)

                           cTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGa                                                                                                            >  1:10538142/1‑140 (MQ=255)

                             ttCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAAt                                                                                                          >  1:4847539/1‑140 (MQ=255)

                                   aTTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGGTGGAGATTATACAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGTCTGCTGGATTTATCTGTTAGAGCAACTGAAAGCCGACGTGCAGGCGGCGAATCTGgcg                                                                                                    >  2:6802519/1‑140 (MQ=255)

                                   aTTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGgcg                                                                                                    >  2:530035/1‑140 (MQ=255)

                                    ttGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGgcgc                                                                                                   <  2:5658965/140‑1 (MQ=255)

                                     tGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCt                                                                                                  >  2:3972674/1‑140 (MQ=255)

                                      gagaTGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCtt                                                                                                 >  2:4394801/1‑140 (MQ=255)

                                       agaTGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTc                                                                                                <  2:4620154/140‑1 (MQ=255)

                                       agaTGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTc                                                                                                >  2:5835141/1‑140 (MQ=255)

                                        gaTGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTcc                                                                                               <  1:8596692/140‑1 (MQ=255)

                                        gaTGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTcc                                                                                               <  1:5139768/140‑1 (MQ=255)

                                        gaTGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTcc                                                                                               <  1:5315076/140‑1 (MQ=255)

                                            tgGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCt                                                                                           >  1:1882835/1‑140 (MQ=255)

                                              gACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTcc                                                                                         >  1:6214688/1‑139 (MQ=255)

                                              gACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCa                                                                                         >  2:9486856/1‑140 (MQ=255)

                                              gACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCa                                                                                         >  1:4262901/1‑140 (MQ=255)

                                              gACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCa                                                                                         <  1:1032790/140‑1 (MQ=255)

                                              gACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGAGTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCa                                                                                         >  1:11072926/1‑140 (MQ=255)

                                                 cGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGACTCTGGCGCTTCCCGCTCACCa                                                                                      >  2:8399476/1‑140 (MQ=255)

                                                  gAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGCGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCAt                                                                                     >  2:10772266/1‑140 (MQ=255)

                                                   aaaaaGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATc                                                                                    >  1:5948536/1‑140 (MQ=255)

                                                   aaaaaGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATc                                                                                    >  2:3506322/1‑140 (MQ=255)

                                                   aaaaaGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATc                                                                                    >  2:1249173/1‑140 (MQ=255)

                                                   aaaaaGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATc                                                                                    >  1:7563056/1‑140 (MQ=255)

                                                   aaaaaGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATc                                                                                    >  1:11457712/1‑140 (MQ=255)

                                                   aaaaaGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGAGTTATATGTGAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTGCCGCTCTCCagc                                                                                    >  2:7053013/1‑138 (MQ=255)

                                                       aGCCAAAGAGCCGGGGCTGGAGATTATGCAGGCGCGCGGTTGGATTGAAGCGCGTATGAAGGAGGCTGGATTTATATGTTAGAGCAACTGACAGCCGACGTGCTGGCGGCGAATCTTGCGCTTCCCGCTCACCATCTGGt                                                                                <  2:6625327/140‑1 (MQ=255)

                                                            aaGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATCGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGTGCGTCCCGCTCACCATCTGGTGACGt                                                                           <  1:7470758/140‑1 (MQ=255)

                                                             agagCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGtt                                                                          >  2:189786/1‑140 (MQ=255)

                                                             agagCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGtt                                                                          >  2:8525092/1‑140 (MQ=255)

                                                                 ccGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCAcc                                                                      <  1:591992/140‑1 (MQ=255)

                                                                  cGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCt                                                                     <  1:8976448/140‑1 (MQ=255)

                                                                   ggTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTg                                                                    >  1:10295457/1‑140 (MQ=255)

                                                                    gTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTgg                                                                   <  1:3635621/140‑1 (MQ=255)

                                                                         ggAGATTATTCAGGCGCGGCGTTGGAGTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGGGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCaa                                                              >  1:5031791/1‑140 (MQ=255)

                                                                               tattCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAg                                                        >  1:5640974/1‑140 (MQ=255)

                                                                               tattCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAg                                                        >  2:6703526/1‑140 (MQ=255)

                                                                               tattCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAg                                                        >  2:264736/1‑140 (MQ=255)

                                                                                 ttCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAgcg                                                      <  1:8860806/140‑1 (MQ=255)

                                                                                 ttCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAgcg                                                      <  1:3478966/140‑1 (MQ=255)

                                                                                 ttCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAgcg                                                      <  1:10016544/140‑1 (MQ=255)

                                                                                  tCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAgcgc                                                     <  2:9329536/140‑1 (MQ=255)

                                                                                  tCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTGAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAgcgc                                                     <  2:980247/140‑1 (MQ=255)

                                                                                   cAGGCGCGGCGTTGGATTGAAGCGCGTCTGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTTGTGACGTTCACCTGGGGCAATGTCAGcgcg                                                    <  1:9802534/140‑1 (MQ=255)

                                                                                    aGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCgg                                                   >  2:7549505/1‑140 (MQ=255)

                                                                                      gcgcGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTa                                                 >  2:922833/1‑140 (MQ=255)

                                                                                      gcgcGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTa                                                 >  2:8893520/1‑140 (MQ=255)

                                                                                      gcgcGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTa                                                 >  1:696104/1‑140 (MQ=255)

                                                                                        gcggcgTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGa                                               <  1:730638/140‑1 (MQ=255)

                                                                                        gcggcgTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGa                                               <  2:1272305/140‑1 (MQ=255)

                                                                                         cggcgTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGAc                                              >  2:6466295/1‑140 (MQ=255)

                                                                                           gcgTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGa                                            >  2:3712907/1‑140 (MQ=255)

                                                                                           gcgTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGa                                            >  2:9045086/1‑140 (MQ=255)

                                                                                             gTTGGATTGAAGCGCGTATGGTGGAGGGTGGTTTTATTTGTTTGCGGAACTGTAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGaaa                                          <  2:3369348/140‑1 (MQ=255)

                                                                                             gTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGaaa                                          <  2:6051677/140‑1 (MQ=255)

                                                                                             gTTGGAGTGACGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCACCGGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGaaa                                          <  2:8148203/140‑1 (MQ=255)

                                                                                               tGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAAcg                                        <  2:10417443/140‑1 (MQ=255)

                                                                                                  aTTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCgg                                     <  1:4780974/140‑1 (MQ=255)

                                                                                                    tGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCa                                   <  1:5457658/140‑1 (MQ=255)

                                                                                                      aaGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAat                                 <  1:1082510/140‑1 (MQ=255)

                                                                                                      aaGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGNCAGCGCGGTAGACGAAACGCGGCAat                                 >  1:6872595/1‑140 (MQ=255)

                                                                                                        gcgcGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAatgg                               >  1:6129307/1‑140 (MQ=255)

                                                                                                          gcgTATGCAGGAGGCTGGATTTATATGTTAGAGCTTCTGAATGCCGACGTGCTTGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGAGGTTGAAGTAACGCGGCTatggat                             <  2:11455916/140‑1 (MQ=255)

                                                                                                          gcgTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAatggat                             <  2:5963994/140‑1 (MQ=255)

                                                                                                          gcgTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAatggat                             <  2:4775861/140‑1 (MQ=255)

                                                                                                           cgTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAatggatg                            >  1:6551934/1‑140 (MQ=255)

                                                                                                           cgTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAatggatg                            >  2:2202467/1‑140 (MQ=255)

                                                                                                           cgTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAatggatg                            >  2:1474761/1‑140 (MQ=255)

                                                                                                           cgTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAatggatg                            >  2:11262478/1‑140 (MQ=255)

                                                                                                            gTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGgcg                                                                                                    <  2:2004571/67‑1 (MQ=255)

                                                                                                                   ggaggCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCaa                    >  2:2319073/1‑140 (MQ=255)

                                                                                                                      ggCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAAcc                 >  2:3858088/1‑140 (MQ=255)

                                                                                                                       gCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACATGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCt                >  2:5137579/1‑140 (MQ=255)

                                                                                                                       gCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCAGCTGGTGACGTTCACCTGGGGCAATGTCCGCGCGGTAGACGAAACGCGGCACTGGATGGTAATCAAACCt                >  2:1088854/1‑140 (MQ=255)

                                                                                                                        cTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCtt               >  1:6448767/1‑140 (MQ=255)

                                                                                                                         tGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCTTc              >  1:1970500/1‑140 (MQ=255)

                                                                                                                         gggATTTATATGTTAGAGCAACGGAAAGCCGACGTGCTGGCGGCTAATCTGGCTCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCTTc              <  2:6213290/139‑1 (MQ=255)

                                                                                                                          ggATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGAGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCGTcc             >  2:7720128/1‑140 (MQ=255)

                                                                                                                           gATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCTTCCg            <  1:1449625/140‑1 (MQ=255)

                                                                                                                            aTTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCTTCCgg           >  2:7369031/1‑140 (MQ=255)

                                                                                                                              ttATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCTTCCGGCg         <  2:1728595/140‑1 (MQ=255)

                                                                                                                               tataTGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCTTCCGGCGt        <  2:3043814/140‑1 (MQ=255)

                                                                                                                                 tatGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCTTCCGGCGTCg      <  2:7622660/140‑1 (MQ=255)

                                                                                                                                  atGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCTTCCGGCGTCGa     >  1:7812585/1‑140 (MQ=255)

                                                                                                                                     ttAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCTTCCGGCGTCGAGtc  >  2:4223421/1‑139 (MQ=255)

                                                                                                                                     ttAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCTTCCGGCGTCGAGTa  >  1:3855583/1‑140 (MQ=255)

                                                                                                                                     ttAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCTTCCGGCGTCGAGTa  >  1:2397790/1‑140 (MQ=255)

                                                                                                                                     ttAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCTTCCGGCGTCGAGTa  >  1:3116197/1‑140 (MQ=255)

CGCTGCATAAACTGAACTATCGCGGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCTTCCGGCGTCGAGTA  >  NZ_CP025268/3751878‑3752150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`