Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 3,894,373 A→G T281A (ACG→GCG)  mdtL → multidrug efflux MFS transporter MdtL

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252683,894,3730AG99.7% 2106.5 / NA 603T281A (ACG→GCG) mdtLmultidrug efflux MFS transporter MdtL
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (336/265);  minor base T (1/1);  total (337/266)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 631 total aligned reads displayed.

TTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGCCGTTTTCATTACGCGCGGGCG  >  NZ_CP025268/3894236‑3894508
                                                                                                                                         |                                                                                                                                       
ttGAGCGCGGTGAATACGCCACCATTCTGGCGCTGACCGCTGGCGTCAGCATGACCGTGTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGgcggcg                                                                                                                                       <  1:8078668/140‑1 (MQ=255)
 tGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGcggcgg                                                                                                                                      >  1:9237966/1‑140 (MQ=255)
 tGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGcggcgg                                                                                                                                      >  1:6323562/1‑140 (MQ=255)
       cgGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCa                                                                                                                                >  2:7645368/1‑140 (MQ=255)
       cgGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCa                                                                                                                                >  1:10371288/1‑140 (MQ=255)
        ggTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCAc                                                                                                                               >  1:4434894/1‑140 (MQ=255)
        ggTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCAc                                                                                                                               >  2:9465102/1‑140 (MQ=255)
                    accaTTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTc                                                                                                                   >  1:8725086/1‑140 (MQ=255)
                    accaTTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTc                                                                                                                   >  1:7200440/1‑140 (MQ=255)
                     ccaTTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCa                                                                                                                  <  2:481500/140‑1 (MQ=255)
                      caTTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTCAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCAc                                                                                                                 >  2:2376980/1‑140 (MQ=255)
                      caTTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCAc                                                                                                                 >  1:1305497/1‑140 (MQ=255)
                      caTTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCAc                                                                                                                 >  1:9095611/1‑140 (MQ=255)
                         tATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCtt                                                                                                              >  2:6943143/1‑140 (MQ=255)
                             gcgcTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCa                                                                                                          <  1:1172937/140‑1 (MQ=255)
                             gcgcTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCa                                                                                                          <  1:3168382/140‑1 (MQ=255)
                             gcgcTGACCGCTGGCGTCAGCATGACCGTTTCATTCACCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCa                                                                                                          >  2:4188510/1‑140 (MQ=255)
                                cTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGc                                                                                                       >  2:2540178/1‑140 (MQ=255)
                                cTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGc                                                                                                       >  2:5155596/1‑140 (MQ=255)
                                cTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTAGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGc                                                                                                       >  1:2376272/1‑140 (MQ=255)
                                cTGACCGCTGGCGTCAGCATGACCGTGTCATTCTCCACGCCCTTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGc                                                                                                       >  2:1291363/1‑140 (MQ=255)
                                   aCCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGt                                                                                                    >  1:7958699/1‑140 (MQ=255)
                                   aCCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGt                                                                                                    >  1:11073959/1‑140 (MQ=255)
                                   aCCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGt                                                                                                    >  2:5248453/1‑140 (MQ=255)
                                   aCCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGt                                                                                                    >  1:5529081/1‑140 (MQ=255)
                                   aCCGCTGGCGTCAGCATGACCGGTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGt                                                                                                    >  1:3425007/1‑140 (MQ=255)
                                     cGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGttt                                                                                                  >  2:7814356/1‑140 (MQ=255)
                                       cTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTtct                                                                                                <  1:6984672/140‑1 (MQ=255)
                                       cTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTtct                                                                                                <  1:1894989/140‑1 (MQ=255)
                                        tGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGACTTGTTCAGCCACGTCCGTTGATGATCACCTCGCAGGTGGTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTtctc                                                                                               >  1:3797500/1‑140 (MQ=255)
                                          gCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACGCTTGCCGTGTCACCTTCCCATGCGGTTTCTCTg                                                                                             >  2:2751516/1‑140 (MQ=255)
                                            gTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGtt                                                                                           <  2:4014189/140‑1 (MQ=255)
                                               aGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTgg                                                                                        >  1:10087238/1‑140 (MQ=255)
                                               aGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTgg                                                                                        >  2:9127903/1‑140 (MQ=255)
                                               aGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTgg                                                                                        >  1:7406882/1‑140 (MQ=255)
                                               aGCAAGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATGTTTACGCCACGTACGTTGATGATCACCTCGCAGGTGTGAGTCCTGGCGGCGGGGATCCCTCGTGCCGTTGCACCTTCCCATGCGGTTGCTCGGGTTgg                                                                                        >  2:7529126/1‑140 (MQ=255)
                                                 cATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTa                                                                                      <  2:5408659/140‑1 (MQ=255)
                                                    gACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCa                                                                                   >  2:10197202/1‑140 (MQ=255)
                                                      ccGTTTCATTCTCCACGCCATTTGCGCTTGGAATTTTTAAGCCACGTACGTTGATGATCACCTAGCATGTGTTATTCCTGGCTTCTGGGATCACTCTTGACGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACg                                                                                 <  1:1087562/140‑1 (MQ=255)
                                                      ccGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACg                                                                                 >  1:676929/1‑140 (MQ=255)
                                                         tttCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTg                                                                              >  1:2636410/1‑140 (MQ=255)
                                                          ttCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCAGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGa                                                                             >  2:7985775/1‑140 (MQ=255)
                                                           tCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGAt                                                                            >  1:6511655/1‑140 (MQ=255)
                                                           tCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGAt                                                                            <  2:11110259/140‑1 (MQ=255)
                                                              ttCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTg                                                                         >  2:3453933/1‑140 (MQ=255)
                                                                 tcCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTgcgc                                                                      >  2:10645092/1‑140 (MQ=255)
                                                                  ccACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGcc                                                                     <  2:6429674/140‑1 (MQ=255)
                                                                  ccACGCCATGTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGcc                                                                     <  1:1597355/140‑1 (MQ=255)
                                                                     cGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGTTCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTGTGTTTGGTATCACGCTGTTTTGCGCCGGt                                                                  >  1:8382034/1‑140 (MQ=255)
                                                                     cGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGt                                                                  >  2:5949396/1‑140 (MQ=255)
                                                                        cATTTGCGCTGGGAATTTTTAAGCCAAGTACGTTGAAGATAACCTCGCAGGTTTTATTCCTGGCTGAGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGTTATCTCTGTTTGGTATAAAGCTGATTTTAGACGGTTtc                                                               >  2:7386079/1‑140 (MQ=255)
                                                                           ttGCGCTGGGACTTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCg                                                            >  2:153729/1‑140 (MQ=255)
                                                                           ttGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCg                                                            >  1:5378094/1‑140 (MQ=255)
                                                                             gcgcTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGt                                                          <  2:3733604/140‑1 (MQ=255)
                                                                              cgcTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTa                                                         <  2:2117518/140‑1 (MQ=255)
                                                                                cTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAgg                                                       >  2:8671547/1‑140 (MQ=255)
                                                                                cTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAgg                                                       <  2:6580474/140‑1 (MQ=255)
                                                                                  gggAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGtt                                                     >  1:6161096/1‑140 (MQ=255)
                                                                                       tttttAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGgt                                                <  2:2842353/140‑1 (MQ=255)
                                                                                       tttttAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACATTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCTCCGGTTTCTCTGTAGGTTTTGgt                                                <  1:10283356/140‑1 (MQ=255)
                                                                                        ttttAAGCCACGTACGTGGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGgtg                                               <  2:10890945/140‑1 (MQ=255)
                                                                                             aGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCg                                          >  2:1811580/1‑140 (MQ=255)
                                                                                             aGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCg                                          >  2:3074050/1‑140 (MQ=255)
                                                                                              gCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGa                                         <  1:2231582/140‑1 (MQ=255)
                                                                                                         tgatgaTCACCTCGCAGGTGTTATTCCTGGCGGCAGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTtct                                                                                                <  1:576288/74‑1 (MQ=255)
                                                                                                         tgatgaTCACCTCGCAGGTGTTATTCCTGGCGGCAGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTtct                                                                                                >  2:576288/1‑74 (MQ=255)
                                                                                                          gatgatCACCTCGCAGGGGTTATTCCTGGCGGCGGGGATCACTCTGGCCGTTTCAGCTTCCGATGCGGTTTCTCTGTTTGGTATCACGCTGTGTTGCGCCGGTGTGTCGGTCGGTGTTGGGGTGGCGATGAGTCAGGCGt                             >  1:4278867/1‑140 (MQ=255)
                                                                                                             gatCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTGTCACCTTCACATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTGTGGGTGTGGCGATTAGTCAGGCGGTAg                          >  1:3053779/1‑140 (MQ=255)
                                                                                                              atCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAgg                         >  1:8883980/1‑140 (MQ=255)
                                                                                                              atCACCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAgg                         >  2:8852522/1‑140 (MQ=255)
                                                                                                                 aCCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTCGGGTTTGGTGTGGCGATGAGTCAGGCGTTAGGGcc                      >  1:503432/1‑140 (MQ=255)
                                                                                                                 aCCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGcc                      >  1:9563386/1‑140 (MQ=255)
                                                                                                                 aCCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGcc                      >  2:1034830/1‑140 (MQ=255)
                                                                                                                 aCCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGcc                      >  2:6190518/1‑140 (MQ=255)
                                                                                                                 aCCTCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGcc                      >  1:756553/1‑140 (MQ=255)
                                                                                                                  ccTCGCAGGTGTTATTCCTGGCGGCGGGNATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCCGGCGTTAGGGCCg                     <  1:6032280/140‑1 (MQ=255)
                                                                                                                   cTCGCAGGTGTTATTCCTGGCGGCGGGGATCACACTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGCCGt                    <  1:11280049/140‑1 (MQ=255)
                                                                                                                    tCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGNCGTTAGGGCCGtt                   <  1:9826856/140‑1 (MQ=255)
                                                                                                                    tCGCAGGTGTTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGCCGtt                   <  1:2128253/140‑1 (MQ=255)
                                                                                                                          gtgtTATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGCCGTTTTCAtt             <  2:5625949/140‑1 (MQ=255)
                                                                                                                           tgtTATTCCTGGCGGCGGGGATCACTCTTGCCGGTTCACCGTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGGAGGTGGTGGTGTGGCGTGGAGTCAGGCGTTAGGGCCGGTTTCATTa            >  2:4659047/1‑140 (MQ=255)
                                                                                                                             ttATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGCCGTTTTCATTAcg          >  2:11299184/1‑140 (MQ=255)
                                                                                                                             ttATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGCCGTTTTCATTAcg          >  1:8561500/1‑140 (MQ=255)
                                                                                                                             ttATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGCCGTTTTCATTAcg          >  1:1407991/1‑140 (MQ=255)
                                                                                                                             ttATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGCCGTTTTCATTAcg          >  1:11237794/1‑140 (MQ=255)
                                                                                                                             ttATTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGCCGTTTTCATTAcg          >  1:10765691/1‑140 (MQ=255)
                                                                                                                               aTTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGCCGTTTTCATTAcgcg        <  2:8454987/140‑1 (MQ=255)
                                                                                                                               aTTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGCCGTTTTCATTAcgcg        <  2:8226793/140‑1 (MQ=255)
                                                                                                                               aTTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGCCGTTTTCATTAcgcg        <  2:956631/140‑1 (MQ=255)
                                                                                                                               aTTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGCCGTTTTCATTAcgcg        <  2:7191227/140‑1 (MQ=255)
                                                                                                                               aTTCCTGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGCCGTTTTCATTAcgcg        <  1:4006387/140‑1 (MQ=255)
                                                                                                                               aTTCCTGGCGGCGGGGATCACTCTTGACGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGTTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGACTTTTTCATTAcgcg        <  1:2911781/140‑1 (MQ=255)
                                                                                                                               aTTCCTGGCGGCGGGGATCACTCGTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGCCGTTTTCATTAcgcg        <  2:8958426/140‑1 (MQ=255)
                                                                                                                               aTTCCGGGCGGCGGGGCTCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACTCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGTGTCAGGCGTTAGGGCCGTTTTCATTAcgcg        <  2:9317421/140‑1 (MQ=255)
                                                                                                                               aTTCCCGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGCCGTTTTCATTAcgcg        <  1:9368787/140‑1 (MQ=255)
                                                                                                                                    tGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGCCGTTTTCATTACGCGCGGGc   >  1:7826783/1‑140 (MQ=255)
                                                                                                                                    tGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGCCGTTTTCATTACGCGCGGGc   <  1:4848218/140‑1 (MQ=255)
                                                                                                                                    tGGCGGCGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGCCGTTTTCATTACGCGCGGGc   <  1:10505788/140‑1 (MQ=255)
                                                                                                                                     ggcggcGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTAt                                                                                     <  1:9491357/57‑1 (MQ=255)
                                                                                                                                     ggcggcGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGCCGTTTTCATTAcgcg        <  2:4399890/134‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                       
TTGAGCGCGGTGAATACGCCACCATTATGGCGCTGACCGCTGGCGTCAGCATGACCGTTTCATTCTCCACGCCATTTGCGCTGGGAATTTTTAAGCCACGTACGTTGATGATCACCTCGCAGGTGTTATTCCTGGCGACGGGGATCACTCTTGCCGTTTCACCTTCCCATGCGGTTTCTCTGTTTGGTATCACGCTGATTTGCGCCGGTTTCTCGGTAGGTTTTGGTGTGGCGATGAGTCAGGCGTTAGGGCCGTTTTCATTACGCGCGGGCG  >  NZ_CP025268/3894236‑3894508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑