BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	4,063,079	A→G	237 (TAA→TAG)	yihL →	GntR family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	4,063,079	0	A	G	99.9%	2720.0 / NA	781	237 (TAA→TAG)	yihL	GntR family transcriptional regulator
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (495/285); minor base C (0/1); total (495/286)
Fisher's exact test for biased strand distribution p-value = 3.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 781 total aligned reads displayed.

CAAGTATCTTGGCGGTTCTGCGGGTATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATAT  >  NZ_CP025268/4062944‑4063214

    tATCTTGGCGGTTCTGCGGGTATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTa                                                                                                                                 >  2:3028469/1‑140 (MQ=255)

    tATCTTGGCGGTTCTGCGGGTATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGAGATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTa                                                                                                                                 >  2:8559342/1‑140 (MQ=255)

       cTTGGCGGTTCTGCGGGTATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATgg                                                                                                                              <  1:8692505/140‑1 (MQ=255)

       cTTGGCGGTTCTGCGGGTATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATgg                                                                                                                              <  2:9093335/140‑1 (MQ=255)

        ttGGCGGTTCTGCGGGTATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGt                                                                                                                             <  2:6097812/140‑1 (MQ=255)

          ggCGGTTCTGCGGGTATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGa                                                                                                                           >  2:4425269/1‑140 (MQ=255)

            cGGTTCTGCGGGTATGCCATCGATATTAATTGTAAAGCATCGCGCCGACCAGAAAGGCAATATTGTCCAAATAGATATTGAATTTTGGAGATTTGAGGCCGTAGACCTCATCATTAATCTGGAGGTGTTTTATGGTGACa                                                                                                                         <  2:3157170/140‑1 (MQ=255)

              gTTCTGCGGGTATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACaat                                                                                                                       >  1:5723786/1‑140 (MQ=255)

                     gggTAAGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAAc                                                                                                                <  1:6755373/140‑1 (MQ=255)

                            ccAGCGATCTTAATTGAAAAGCATCGCGCCGACCCGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCaagaa                                                                                                         <  2:7689442/140‑1 (MQ=255)

                            ccAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCaagaa                                                                                                         <  2:5548944/140‑1 (MQ=255)

                            ccAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCaagaa                                                                                                         <  2:8644894/140‑1 (MQ=255)

                            ccAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCaagaa                                                                                                         <  2:6770919/140‑1 (MQ=255)

                            ccAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCaagaa                                                                                                         <  2:8905262/140‑1 (MQ=255)

                            ccAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCaagaa                                                                                                         <  2:6960948/140‑1 (MQ=255)

                              aGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCaagaaag                                                                                                       <  1:5594575/140‑1 (MQ=255)

                                  aTCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTc                                                                                                   >  2:10993360/1‑140 (MQ=255)

                                    cTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTa                                                                                                 >  1:7793125/1‑140 (MQ=255)

                                           gAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTACAAATAGATATTGAATATTGGCGATTTGAGGACGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACgtg                                                                                          >  2:6482547/1‑140 (MQ=255)

                                              aaGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCg                                                                                       >  2:10054950/1‑140 (MQ=255)

                                               aGCATCGCGCCGACCATCAAGGCAATATTGTCCAAATAGATATGGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGa                                                                                      <  2:1304518/140‑1 (MQ=255)

                                                 cATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGaca                                                                                    <  2:4024702/140‑1 (MQ=255)

                                                 cATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGaca                                                                                    <  1:3955607/140‑1 (MQ=255)

                                                 cATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGaca                                                                                    >  2:1056719/1‑140 (MQ=255)

                                                           aCCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCctct                                                                          >  1:5928007/1‑140 (MQ=255)

                                                               gCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCtttat                                                                      <  1:322418/140‑1 (MQ=255)

                                                                  aGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCtttattta                                                                   <  2:2427383/140‑1 (MQ=255)

                                                                  aGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCtttattta                                                                   <  1:10054951/140‑1 (MQ=255)

                                                                   ggCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTAc                                                                  <  2:6269025/140‑1 (MQ=255)

                                                                   ggCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTAc                                                                  >  1:11192891/1‑140 (MQ=255)

                                                                    gCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACa                                                                 <  2:4558058/140‑1 (MQ=255)

                                                                     cAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTATACCTCATCATTAATCTGTAGGTGTTTTATGGGGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACAt                                                                >  1:4922275/1‑140 (MQ=255)

                                                                     cAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCtt                                                                               >  2:2191167/1‑125 (MQ=255)

                                                                     cAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCtt                                                                               <  1:2191168/125‑1 (MQ=255)

                                                                     cAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACAt                                                                >  1:9313991/1‑140 (MQ=255)

                                                                       atatTGTCCAAATAGATATTGAATATTGTCGATTTGAGGCAGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGc                                                              <  2:11239619/140‑1 (MQ=255)

                                                                        tatTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCt                                                             >  1:4218418/1‑140 (MQ=255)

                                                                         atTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCtt                                                            >  2:2286445/1‑140 (MQ=255)

                                                                         atTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCtt                                                            >  1:10545257/1‑140 (MQ=255)

                                                                          ttGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTa                                                           >  1:7615142/1‑140 (MQ=255)

                                                                          ttGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTa                                                           <  2:4220172/140‑1 (MQ=255)

                                                                          gtGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTGTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTa                                                           >  2:2934945/2‑140 (MQ=255)

                                                                             tCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTGATCTGTAGGTGTTTTATGGTGACAATAAATATCGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCGTATGc                                                        >  1:6153993/1‑140 (MQ=255)

                                                                             tCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCtttattta                                                                   <  1:432535/129‑1 (MQ=255)

                                                                             tCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGc                                                        >  2:1171968/1‑140 (MQ=255)

                                                                             tCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGc                                                        >  1:449963/1‑140 (MQ=255)

                                                                             tCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGc                                                        >  2:7891170/1‑140 (MQ=255)

                                                                             tCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGCGTCGACACTCTTCCTCTTTATTTACATGCTTATGc                                                        >  1:10848475/1‑140 (MQ=255)

                                                                               cAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTCGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCGTCCTCTTTATTTACATGCTTATGCCt                                                      <  2:7537351/140‑1 (MQ=255)

                                                                               cAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCt                                                      >  2:5878491/1‑140 (MQ=255)

                                                                               cAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCGGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCt                                                      >  2:9666050/1‑140 (MQ=255)

                                                                               cAAATAGATATAGAATATTGGCGATTAGAGGCCGTAGACCTCATCATTAATCTGGAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATGTACATGCTTATGCCt                                                      <  2:9285645/140‑1 (MQ=255)

                                                                                  aTAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTc                                                   >  1:2951473/1‑140 (MQ=255)

                                                                                      atatTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGGAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCAttt                                               >  2:8398813/1‑140 (MQ=255)

                                                                                       tatTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCttttc                                              >  2:8164097/1‑139 (MQ=255)

                                                                                       tatTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTa                                              >  2:3412466/1‑140 (MQ=255)

                                                                                           gAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAata                                          >  1:11400002/1‑140 (MQ=255)

                                                                                           gAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAata                                          <  1:9191291/140‑1 (MQ=255)

                                                                                            aaTATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCaagaa                                                                                                         >  2:4820921/1‑76 (MQ=255)

                                                                                            aaTATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAatat                                         >  1:112909/1‑140 (MQ=255)

                                                                                            aaTATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAatat                                         >  1:979756/1‑140 (MQ=255)

                                                                                             atatTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATg                                        <  1:2024739/140‑1 (MQ=255)

                                                                                                ttGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCt                                                      <  2:1529258/123‑1 (MQ=255)

                                                                                                ttGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCgg                                     >  1:4619512/1‑140 (MQ=255)

                                                                                                ttGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCgg                                     <  1:3769971/140‑1 (MQ=255)

                                                                                                 tGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGc                                    >  2:10803326/1‑140 (MQ=255)

                                                                                                  ggCGATTTGAGGCCGTAGACCTCATCTTTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCt                                   <  1:8147942/140‑1 (MQ=255)

                                                                                                  ggCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCt                                   <  2:979756/140‑1 (MQ=255)

                                                                                                   gCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTg                                  >  1:5451394/1‑140 (MQ=255)

                                                                                                      aTTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCtttattta                                                                   <  1:2404168/104‑1 (MQ=255)

                                                                                                      aTTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGaa                               >  1:1556630/1‑140 (MQ=255)

                                                                                                      aTTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGaa                               >  2:2663091/1‑140 (MQ=255)

                                                                                                      aTTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGaa                               >  1:7417176/1‑140 (MQ=255)

                                                                                                        ttGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAag                             >  1:3606248/1‑140 (MQ=255)

                                                                                                        ttGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAag                             >  1:1188881/1‑140 (MQ=255)

                                                                                                           aGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAgt                          <  1:6621140/140‑1 (MQ=255)

                                                                                                              ccGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGtt                       <  1:5262292/140‑1 (MQ=255)

                                                                                                                 tAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGcc                    >  2:7186097/1‑140 (MQ=255)

                                                                                                                 tAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTGCATTTAAATATGCGGCTGGAAAGAGGGTTGcc                    >  1:3058896/1‑140 (MQ=255)

                                                                                                                   gACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCctg                  <  1:3412467/140‑1 (MQ=255)

                                                                                                                     ccTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATg                                        >  1:8980786/1‑116 (MQ=255)

                                                                                                                        catcatTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCaagaaa                                                                                                        <  2:10229580/49‑1 (MQ=255)

                                                                                                                        catcatTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGAt             <  1:1749506/140‑1 (MQ=255)

                                                                                                                        catcatTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGAt             >  1:2736579/1‑140 (MQ=255)

                                                                                                                        catcatTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGAt             >  1:4768601/1‑140 (MQ=255)

                                                                                                                        catcatTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGAt             >  1:851985/1‑140 (MQ=255)

                                                                                                                        catcatTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATGCTACAACGTGTCGACACTCTTCCTCCTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGAt             >  1:7123970/1‑140 (MQ=255)

                                                                                                                         atcatTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGAt                                                                                                     <  1:7919174/51‑1 (MQ=255)

                                                                                                                         atcatTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCtttattta                                                                   >  1:1021953/1‑85 (MQ=255)

                                                                                                                         atcatTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGAtt            >  2:5030200/1‑140 (MQ=255)

                                                                                                                         atcatTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGAtt            >  2:1873937/1‑140 (MQ=255)

                                                                                                                         atcatTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGAtt            >  1:7247082/1‑140 (MQ=255)

                                                                                                                          tcatTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGAttt           >  2:5707232/1‑140 (MQ=255)

                                                                                                                          tcatTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGAttt           >  1:1353247/1‑140 (MQ=255)

                                                                                                                           catTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTa          >  1:628153/1‑140 (MQ=255)

                                                                                                                              tAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACtt       >  2:3715893/1‑140 (MQ=255)

                                                                                                                              tAATCTGTAGGTGTTTTATGGAGACAATAAATAACTCAAGAAAGATTCTACAACGTGTCGACACTATTCCTCTATATTTACATGCTAATGCCTTTCATTTAAATATGCGGCTGGAAATAGTGTTGCCTGCTGATTTACtt       >  1:10307183/1‑140 (MQ=255)

                                                                                                                                aTCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGa     >  2:104270/1‑140 (MQ=255)

                                                                                                                                aTCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTATCCAACGTGTCGACACTCTTCCTCTTTATTTACATGCTGATGACTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGa     >  2:11343971/1‑140 (MQ=255)

                                                                                                                                   tGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTc                                                                              <  1:9577880/64‑1 (MQ=255)

CAAGTATCTTGGCGGTTCTGCGGGTATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACTTGATAT  >  NZ_CP025268/4062944‑4063214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`