BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	4,608,154	G→A	noncoding (48/87 nt)	CXP41_RS23870 ←	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	4,608,154	0	G	A	97.3%	1351.5 / NA	426	noncoding (48/87 nt)	CXP41_RS23870	tRNA‑Leu
Reads supporting (aligned to +/- strand): ref base G (3/4); major base A (216/186); minor base T (4/7); total (223/203)
Fisher's exact test for biased strand distribution p-value = 3.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 426 total aligned reads displayed.

CCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGC  >  NZ_CP025268/4608017‑4608290

cccccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCACTTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGACGCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCACACGTCCGTAAGTac                                                                                                                                        >  2:10240559/1‑140 (MQ=25)

  cccACGTCCGTAAGGACACTCACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGAGGTGGACCGCCACGTCCGTACGAacac                                                                                                                                      >  2:8840948/1‑140 (MQ=25)

   ccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTNATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACt                                                                                                                                     <  1:6041606/140‑1 (MQ=255)

   ccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACt                                                                                                                                     <  1:1084180/140‑1 (MQ=255)

     aCGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTaa                                                                                                                                   <  1:4920443/140‑1 (MQ=255)

     aCGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTaa                                                                                                                                   <  1:4354876/140‑1 (MQ=255)

        tCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTATGATATCGCCGCGAAGGGTGCGA‑GGGGGGGGCCGTGAACCCCCACGACCGAAAGAACACTAacac                                                                                                                                >  2:11047945/1‑140 (MQ=21)

         ccGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACAcc                                                                                                                               <  1:1354291/140‑1 (MQ=255)

             aaGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGaa                                                                                                                           >  1:9425576/1‑140 (MQ=255)

                 acacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTaa                                                                                                                                   >  1:1063873/1‑128 (MQ=255)

                 acacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCCTCGCACAGTCATCTTACTTTAATTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCTCGTCCTAACGGACACTAACACCTGCAGCTa                                                                                                                       >  2:7044612/1‑140 (MQ=37)

                   acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTCgc                                                                                                                     >  2:9536700/1‑140 (MQ=255)

                   acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgc                                                                                                                     >  1:466951/1‑140 (MQ=255)

                   acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGACTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAAAACCTGAAGCTAgc                                                                                                                     >  2:926300/1‑140 (MQ=255)

                    cTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCGCGTCCGTAAGAACACTACCACCTGAAGCTAgcg                                                                                                                    >  2:7418027/1‑140 (MQ=255)

                    cTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgcg                                                                                                                    <  2:1492675/140‑1 (MQ=255)

                       acacCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGGTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAAAACTAACACCTGAAGCTAGCGCGt                                                                                                                 >  1:1240694/1‑140 (MQ=255)

                         acCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTACCTCCTGAAGCTAGCGCGTCt                                                                                                               >  1:11068923/1‑140 (MQ=255)

                          ccTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGTGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTa                                                                                                              >  2:5723138/1‑140 (MQ=255)

                           cTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGGCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGCCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAc                                                                                                             >  1:3780793/1‑140 (MQ=255)

                            tGAAGCTAGCGCTTATACCAATTCCGACAACTTCGCACAGTCATCTTACTTTTTTTGATATCTCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAcc                                                                                                            <  2:10653818/140‑1 (MQ=25)

                               aGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTGTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGATCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCact                                                                                                         >  1:964869/1‑138 (MQ=255)

                                   aGCTCGTATACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                                                                                                     <  2:6170830/140‑1 (MQ=255)

                                   aGCGCGTCTACATATTCCGCCACCTTCGCTCAGTCATCTTTCTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCAAGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                                                                                                     <  2:6862989/140‑1 (MQ=21)

                                   aGCGCGACTACCAATTCCGCCACCTTCGCACAGAAATATTACTTTTTTTGATATCGCATCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAATAAAACTAACACCTGAAGCTAGCGCGTCTACAAATTCCg                                                                                                     >  2:4256464/1‑140 (MQ=21)

                                    gcgcGTCTACCTATTCCGCCAACTTCGCACAGTCATCTTACATTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                                                    <  1:890824/140‑1 (MQ=255)

                                    gcgcGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                                                    <  1:90477/140‑1 (MQ=255)

                                      gcgTCTACCCATTCCGCCACCTTCGCACAGTCAACTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa                                                                                                  <  1:7869045/140‑1 (MQ=255)

                                        gTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAcc                                                                                                >  1:3147820/1‑140 (MQ=255)

                                              ccagaccGCCACCTACGCACAGTCAACTTACATTTTTTGCTATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                          <  1:3441186/135‑1 (MQ=18)

                                              ccaTTCCGCCACCTTCTCACATTCATCTTACTTTATTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                          <  2:9116184/138‑1 (MQ=18)

                                              ccaTTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                          <  1:3285085/138‑1 (MQ=255)

                                              cacttCCGCCACCTTCGCACAGGCAACTGACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                          <  1:7207745/137‑1 (MQ=18)

                                              cAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAAGTCCGCCACCTTCGcc                                                                                          <  2:5480484/140‑1 (MQ=255)

                                              cAATTCCGCCACCTTCGCACAGTCATCTTACTTTTATTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                          <  2:10295891/140‑1 (MQ=255)

                                              cAATTCCGCCAACTTCGCACATTCATCTTACTTTTTTTGATATCGCCTCTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                          <  1:2077018/140‑1 (MQ=255)

                                               aaTTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGccc                                                                                         <  1:3951992/140‑1 (MQ=255)

                                                 ttCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcatac                                                                                       >  1:10329596/1‑136 (MQ=255)

                                                  tCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGTGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGAGCGTCTACCAAATCCGCCACCTTCGAACAGt                                                                                      >  2:11147059/1‑140 (MQ=21)

                                                  tCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTACGCCCAGt                                                                                      >  2:7911140/1‑140 (MQ=255)

                                                   ccGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg                                                                                     >  2:659879/1‑140 (MQ=255)

                                                   ccGCCACCTTCGCACAGTCAACTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg                                                                                     <  1:11404170/140‑1 (MQ=255)

                                                    cGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGc                                                                                    >  2:3235378/1‑140 (MQ=255)

                                                      ccACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTCTGAACACTATCACCTGAAGCTAGCGCGTGTCCCACTTCCGCCACCTTCGCGCCGTgaga                                                                                  >  2:10850355/1‑140 (MQ=18)

                                                      ccACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACt                                                                                                                                     <  2:4085315/89‑1 (MQ=255)

                                                       cACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                                                                 <  2:8914082/140‑1 (MQ=255)

                                                       cACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                                                                 <  1:9286847/140‑1 (MQ=255)

                                                       cACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                                                                 <  1:2736079/140‑1 (MQ=255)

                                                       cACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                                                                 <  2:8072846/140‑1 (MQ=255)

                                                            tCGCACAGTCATCTTACTTTTGTTGATATCGCCTCGGTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCAAGTGCGAGCAata                                                                            >  2:28002/1‑140 (MQ=255)

                                                               cacaGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATc                                                                          <  1:6403214/140‑1 (MQ=255)

                                                                   gTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGt                                                                     <  1:5223181/140‑1 (MQ=255)

                                                                    tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTg                                                                    >  1:10068048/1‑140 (MQ=255)

                                                                       tCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCAACTGCGAGCAATATCAACGTGttt                                                                 >  2:7766881/1‑140 (MQ=255)

                                                                        cTTACTTTTTTTGATATCGCCTCGTGTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGttt                                                                <  1:158039/140‑1 (MQ=255)

                                                                            ctgtttttGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCACTATCAAAGTGGTTTTTgg                                                            >  2:2975000/4‑140 (MQ=255)

                                                                            cTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGAGAGCACTATCAACGAGGTTTTTgg                                                            >  2:7581223/1‑140 (MQ=255)

                                                                            cTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTAGAACCACCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCACTTCCGCCCCCTTCGCCCAGTGCGAGCTTTATCTAGGTGGTGTTTgg                                                            >  2:10026003/1‑140 (MQ=18)

                                                                              ttttttGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAGTTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTg                                                          >  2:9703429/1‑140 (MQ=255)

                                                                              ttttttGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTg                                                          >  1:2250964/1‑140 (MQ=255)

                                                                              ttttttGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGAGGTTTTTGGTg                                                          >  2:3372969/1‑140 (MQ=255)

                                                                              ttttttGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGGCTACCAATTCCGCCACCTTCGCACACAGCGAGCTATATCAACGTGGTTTTTGGTg                                                          >  1:2146517/1‑140 (MQ=18)

                                                                                 tttGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGa                                                       >  1:3018699/1‑140 (MQ=255)

                                                                                 ttagATATAGCTTCGTTTGGTGCTA‑TGGGGGGGACTTGAACCCCCACGTACTGAAGAAATCATCCACATGAATCTTGCGAGTATTCCTATTACTACACCCACGCACATTGCTAGCAATCTCAACTAGGTATTTGGTGCGa                                                       <  2:3674380/137‑1 (MQ=255)

                                                                                  ttGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAg                                                      <  1:648626/140‑1 (MQ=255)

                                                                                  ttGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATAACAACGTGGTTGTAGGGGCGAg                                                      >  1:564406/1‑140 (MQ=18)

                                                                                  ttGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGAATTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGAGCGAGCACTATCAACGTGGTTATTGGTGCGAg                                                      >  1:3639995/1‑140 (MQ=18)

                                                                                      attCGCCTCGTTTGGTGCGA‑GGGGGGGGACATGAACCCCCACGTCCGTAAGAACACTATCACCTGCAGCTAGCTCGTCTACCACTTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAggggg                                                  <  2:5930623/138‑1 (MQ=21)

                                                                                       atCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCACTTCCGCCACCTTCGCCCAGTGCGAGCACTATCAACGTGGTTTTTGGTGCGAgggggg                                                 >  2:1150594/1‑140 (MQ=255)

                                                                                         cGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGa                                                       >  2:2622996/1‑132 (MQ=255)

                                                                                         cGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGa                                                       <  1:2622997/132‑1 (MQ=255)

                                                                                               gTTTGGTGCGC‑GGGGGGGGACAAGAACCCCCACGTCCGAAAGAACACTAACACCTGAAGCTAGCGCGTCTACCCATTCCGCCACCTTCGCCCAGTTCTAGCTATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGa                                         <  1:5789083/140‑1 (MQ=18)

                                                                                                tttGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGaa                                        >  2:5278040/1‑140 (MQ=255)

                                                                                                     tgagA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATACCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTGGTAGGTCCGAGGGGGGGTACTTTAaccacc                                   >  2:6904229/4‑140 (MQ=18)

                                                                                                     tGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCAAGTGCCAGCACTAAAAACGTGGTTATTGGTGCGAGGCGGGGGACTTGAAacccc                                   >  2:8666033/1‑140 (MQ=18)

                                                                                                      gCGA‑GGGGGGGGACATGAACCCCCACGTCAGTAAGAACACTAACACCTGAAGCTAGCGCTTCTACAATTTCCGCCACCATCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCa                                  <  2:165744/140‑1 (MQ=25)

                                                                                                        gA‑GGGGGGGGACTTGACCCCCCACGTCCGTAAGAACACTACCACCTGACGCTAGCGCGTCTACCAAGTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACg                                <  1:11168133/140‑1 (MQ=25)

                                                                                                        gA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACg                                <  1:7454819/140‑1 (MQ=255)

                                                                                                         gtggggggggACTTGAACCCCCACGTCCGTAAGTACACTATCACCTGAAGCTAGCGCGTCTACCATTTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACg                                <  2:8983490/138‑1 (MQ=255)

                                                                                                           ggggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTc                              >  1:2928107/1‑140 (MQ=255)

                                                                                                            gggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTcc                             >  1:9854108/1‑140 (MQ=255)

                                                                                                              gggggACTTGAACCCCCACGTCCGTATGAACACTACCACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGt                           <  1:8230604/140‑1 (MQ=255)

                                                                                                               ggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCACTATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTa                          >  2:8492182/1‑140 (MQ=255)

                                                                                                               gggcACATGCACCCCCACTTCCGGAAGCACACTAACACCTGAAGATAGCGCGTCAACCCCTTACTCAACCCACGCCCACTGCGAGCAATCTCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTa                          <  1:7663788/140‑1 (MQ=21)

                                                                                                                gggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGaaa                         >  2:1300751/1‑140 (MQ=255)

                                                                                                                gggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTaa                         >  2:9879901/1‑140 (MQ=255)

                                                                                                                gggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTaa                         >  1:10151794/1‑140 (MQ=255)

                                                                                                                  gACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAAGTCCGCCCCCTTCGCCCAGTGCGTGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAgg                       <  1:4158989/140‑1 (MQ=255)

                                                                                                                  gACTTGAACCCCCACGTCCGTAAGAACACGAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAgg                       <  1:10728083/140‑1 (MQ=255)

                                                                                                                    cTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGac                     >  2:2367789/1‑140 (MQ=255)

                                                                                                                      tGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGTGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGacac                   >  1:4764950/1‑140 (MQ=255)

                                                                                                                      tGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCACTATCAACGTGGTGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTCAGGacac                   >  2:11339647/1‑140 (MQ=255)

                                                                                                                       gAACCCGCACGTCTTTAAGAACACTATCAGCTGAAGCTTTCGCGTCTACCTATACCGACACCTTCGCCCAGTGCGAGCAATATATACGTGGTTATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACt                  <  2:6365301/140‑1 (MQ=21)

                                                                                                                       gAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACt                  >  2:5133119/1‑140 (MQ=255)

                                                                                                                        aaCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTa                 <  2:4535525/140‑1 (MQ=255)

                                                                                                                            cccACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAacac             >  1:4594403/1‑140 (MQ=255)

                                                                                                                                cGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGa         >  1:6863939/1‑140 (MQ=255)

                                                                                                                                  tCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAg       >  1:8459850/1‑140 (MQ=255)

                                                                                                                                    cGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCt     >  2:4712547/1‑140 (MQ=255)

                                                                                                                                       aaGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgc  >  1:10464419/1‑140 (MQ=255)

CCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGC  >  NZ_CP025268/4608017‑4608290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`