BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	442,101	T→C	197 (TAA→TAG)	yajL ←	protein deglycase YajL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	442,101	0	T	C	99.7%	1215.6 / NA	362	197 (TAA→TAG)	yajL	protein deglycase YajL
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (191/170); minor base A (0/1); total (191/171)
Fisher's exact test for biased strand distribution p-value = 4.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 363 total aligned reads displayed.

TGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGC  >  NZ_CP025268/441964‑442236

tgtgAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTa                                                                                                                                       >  1:8413952/1‑140 (MQ=255)

tgtgAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTa                                                                                                                                       <  1:7528315/140‑1 (MQ=255)

    aGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAAGAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCTTCTGGTCAGATGCGACGTGTGCCGCATCCGAGACTACTCg                                                                                                                                   >  2:9476190/1‑140 (MQ=255)

    aGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCg                                                                                                                                   >  2:6819279/1‑140 (MQ=255)

    aGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATGTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCg                                                                                                                                   >  2:4066409/1‑140 (MQ=255)

      cgcgGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGta                                                                                                                                 <  2:4318593/140‑1 (MQ=255)

        cgGGGTGATGAGCCGTCTGCAGGCGCTCGATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGtaat                                                                                                                               <  1:8832789/140‑1 (MQ=255)

        cgGGGTGATGAGCAGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGtaat                                                                                                                               <  2:906688/140‑1 (MQ=255)

         ggggTGCTGAGCCGGCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGtaata                                                                                                                              <  2:1616121/140‑1 (MQ=255)

          gggTGATGAGCCGTCTGTAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGGCATTTGTGGTTTTTACGGCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGtaataa                                                                                                                             >  1:4268496/1‑140 (MQ=255)

          gggTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTCCGCGtaataa                                                                                                                             >  1:1571152/1‑140 (MQ=255)

          gggTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGtaataa                                                                                                                             >  1:9756197/1‑140 (MQ=255)

          gggTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGtaataa                                                                                                                             <  2:2822332/140‑1 (MQ=255)

          gggTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGtaataa                                                                                                                             >  1:5055184/1‑140 (MQ=255)

          gggTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCGTTAACAATGTGCAGGTATAGCGCCCGTCAGTTGTGGTGTGTACGTCGCATCTGGTCCGATGCGACGTTTGCCGCCTCCGACCCTACTCGgaatca                                                                                                                             >  2:6216199/1‑138 (MQ=255)

              gatgaGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATtat                                                                                                                         <  2:551299/140‑1 (MQ=255)

               atgaGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATtata                                                                                                                        <  1:9282211/140‑1 (MQ=255)

                tgaGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATaa                                                                                                                       <  2:5944152/140‑1 (MQ=255)

                     cGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATccc                                                                                                                  <  2:8778889/140‑1 (MQ=255)

                     cGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATcca                                                                                                                  >  2:10094656/1‑139 (MQ=255)

                       tCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTg                                                                                                                >  1:4241168/1‑140 (MQ=255)

                         tGCAGGCGCTCTATCTGCGCGAGCCGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACGCGTAATAATTATAAATCCCTgcc                                                                                                              >  1:7314071/1‑140 (MQ=255)

                           cAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATGTGTGGTGTTTACGTCGCAGCTGGTCAGATGCGACGGTTGCCGCATCCGACACGACTCGTAATAATTCTAAATCCCTgccgc                                                                                                            >  2:11187141/1‑140 (MQ=255)

                            aGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGTTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTgccgcc                                                                                                           <  2:2948331/140‑1 (MQ=255)

                              gcgcTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCAt                                                                                                         <  2:6945256/140‑1 (MQ=255)

                              gcgcTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCAt                                                                                                         >  1:348416/1‑140 (MQ=255)

                                 ctctATCTGCGCGAGCAGGGCTTTAACACTGTGAAGGTATATCGCCCGTAAGTTGTGGTTTTTACGTCGCATCTGGTCCGATGCGACGTTTGCCGCATCCGACACTACTCGTCATAATTATAAATCCCTGCCGCCATCAc                                                                                                      >  2:10645370/1‑140 (MQ=255)

                                 ctctATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCAc                                                                                                      >  2:3827528/1‑140 (MQ=255)

                                 ctctATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTGTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCAc                                                                                                      >  1:1971898/1‑140 (MQ=255)

                                   ctATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCa                                                                                                    >  2:4641244/1‑140 (MQ=255)

                                       cTGCGCGAGCAGGGCTTTACCAAGGTGACGGAATAACGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGCCGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTtg                                                                                                <  1:6982105/140‑1 (MQ=255)

                                        tGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTtgt                                                                                               >  1:7220859/1‑140 (MQ=255)

                                         gcgcGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTtgtg                                                                                              >  1:6533215/1‑140 (MQ=255)

                                          cgcgAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGa                                                                                             >  1:5677432/1‑140 (MQ=255)

                                          cgcgAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTAGTGa                                                                                             >  1:11291783/1‑140 (MQ=255)

                                            cgAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATg                                                                                           >  2:10926117/1‑140 (MQ=255)

                                            cgAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATg                                                                                           >  2:7852824/1‑140 (MQ=255)

                                             gAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGc                                                                                          <  1:438478/140‑1 (MQ=255)

                                             gAGCAGGGCATTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATACATCCCTGCCGCCATCACCAGTTGTGATGc                                                                                          <  1:1424208/140‑1 (MQ=255)

                                              aGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGcc                                                                                         <  1:6794825/140‑1 (MQ=255)

                                              aGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGcc                                                                                         <  1:10768970/140‑1 (MQ=255)

                                                cAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTgccgcc                                                                                                           <  1:10563926/120‑1 (MQ=255)

                                                    gCTTTAACAATGTGAAGGTATATCGCCAGTAATTTGTGGTTTTTACGTCGCATCTGGTCATATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCa                                                                                   <  2:3625611/140‑1 (MQ=255)

                                                             aTGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCtttt                                                                          >  1:6708949/1‑140 (MQ=255)

                                                             aTGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCtttt                                                                          >  2:1046243/1‑140 (MQ=255)

                                                             aTGTGAAGGTATATCGCCCGTAAGTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCGGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCtttt                                                                          >  1:6151128/1‑140 (MQ=255)

                                                              tgtgACGGGATATCGCCCGGGAGGTGTGGTTTGTACGGCGCATCTGGTCAGATGCGACGTGTGCCGCATCCGACACGACGCGTATTAGGTCTATTTCCCTGCCGCCATCACCCGTTGTGCTGCCCCTGGATGGGGtgttt                                                                         >  2:8883556/1‑140 (MQ=255)

                                                              tgtgAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCttttt                                                                         >  2:310432/1‑140 (MQ=255)

                                                              tgtgAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCttttt                                                                         >  2:7670018/1‑140 (MQ=255)

                                                               gtgAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCTTCACCAGTTGTGATGCCACTTCATGGGCTTTTTc                                                                        >  2:7505897/1‑140 (MQ=255)

                                                               gtgAAGGTATATCGCCCGGAATGTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTc                                                                        >  1:8692110/1‑140 (MQ=255)

                                                                tgAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCTCATCTGGTCAGATGAGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGAAGTGATGCCACTTCATGGGCTTTTTCa                                                                       >  2:9918963/1‑140 (MQ=255)

                                                                 gAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCAc                                                                      <  1:9542408/140‑1 (MQ=255)

                                                                 gAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCAc                                                                      <  2:9798542/140‑1 (MQ=255)

                                                                  aaGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATACTTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACg                                                                     <  1:1145152/140‑1 (MQ=255)

                                                                  aaGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTATTCACg                                                                     <  2:7380831/140‑1 (MQ=255)

                                                                   cggTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGc                                                                    <  1:3236262/139‑1 (MQ=255)

                                                                   aGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGc                                                                    <  1:2638346/140‑1 (MQ=255)

                                                                    ggTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGcc                                                                   <  2:6332283/140‑1 (MQ=255)

                                                                     gTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGccc                                                                  <  2:5359347/140‑1 (MQ=255)

                                                                        tatCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGGTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGAccaac                                                               >  2:1270920/1‑140 (MQ=255)

                                                                            gCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCcaaccaac                                                           >  2:8518279/1‑140 (MQ=255)

                                                                               cGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTACTAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAgg                                                        >  2:9570984/1‑140 (MQ=255)

                                                                               cGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAgg                                                        >  1:4671621/1‑140 (MQ=255)

                                                                               cGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTGTCACGCCCAACCAACAgg                                                        >  2:2092378/1‑140 (MQ=255)

                                                                                gTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGt                                                       >  2:4155191/1‑140 (MQ=255)

                                                                                gTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGt                                                       >  2:7350653/1‑140 (MQ=255)

                                                                                        tgGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGt                                                       >  2:11297796/1‑132 (MQ=255)

                                                                                        tgGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAAtt                                               >  1:5499719/1‑140 (MQ=255)

                                                                                          gTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGTTGCCACTTCATGGGCTTTTTCACGCCCAGCCGACAGGTCGTTAtttgt                                             >  1:10115676/1‑138 (MQ=255)

                                                                                          gTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAAtttt                                             <  2:2435903/140‑1 (MQ=255)

                                                                                                 cGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCa                                      <  2:7115625/140‑1 (MQ=255)

                                                                                                    cGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAg                                   >  2:10429495/1‑140 (MQ=255)

                                                                                                      cATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTc                                 >  2:8114550/1‑140 (MQ=255)

                                                                                                        tCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGa                               <  2:5677431/140‑1 (MQ=255)

                                                                                                        tCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGa                               <  2:10839386/140‑1 (MQ=255)

                                                                                                        tCTGGTCAGATGCGACGATTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGa                               <  2:6548122/140‑1 (MQ=255)

                                                                                                           ggTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAg                            <  2:9664930/140‑1 (MQ=255)

                                                                                                            gTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGc                           <  2:1346427/140‑1 (MQ=255)

                                                                                                             tCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCt                          <  2:1244238/140‑1 (MQ=255)

                                                                                                                 aTGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAAATTTCAGACCAAAGTCGATAGCTGTAc                      >  2:363518/1‑140 (MQ=255)

                                                                                                                  tGCGCCGTTTGCCGCAGCCGACACGACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGCTTTGCTGCCCCTGCAGGGGCATTTTCACGCCCAACCCACAGGTCGATAATTTTCAGACCATAGTCGATAGCTGGAcc                     <  2:4934685/140‑1 (MQ=255)

                                                                                                                  tGCGACGTTTGCCGCATCCGACACTACTCGTCATAATTATAAATCCCTGCCGCCAGCCCCAGTGGTGCTGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCCGACCAAAGTCGATAGCTGTAcc                     <  2:5102004/140‑1 (MQ=255)

                                                                                                                  tGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTAcc                     <  1:4066410/140‑1 (MQ=255)

                                                                                                                  tGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTAcc                     <  1:10868375/140‑1 (MQ=255)

                                                                                                                  agCGACGTTTGCCGCAACCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTACATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTAcc                     <  2:11407797/139‑1 (MQ=255)

                                                                                                                    cGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAATGTCGATAGCTGTACCCg                   >  1:9337519/1‑140 (MQ=255)

                                                                                                                    cGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCg                   >  1:8672178/1‑140 (MQ=255)

                                                                                                                      aCGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGAGACTTTTCAGACCAAAGTCGATAGCTGTACCCGGc                 >  2:7995149/1‑140 (MQ=255)

                                                                                                                        gattGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGccc               <  2:10189364/138‑1 (MQ=255)

                                                                                                                           tGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCctg            >  2:3522208/1‑140 (MQ=255)

                                                                                                                           tGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCctg            >  1:9923507/1‑140 (MQ=255)

                                                                                                                           tGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCctg            >  1:11115936/1‑140 (MQ=255)

                                                                                                                               gCATCCGACACTACTCGTAATAATGATACATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCctggctg        <  2:9161724/140‑1 (MQ=255)

                                                                                                                                 aTCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGGCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGt      >  1:10306396/1‑140 (MQ=255)

                                                                                                                                   ccGACACTACTCGTAATACTTATAAATCCCAGCCGCCATCACCAGTTGTGATGCCACTTCCTGGGCTTTTTCACGCCCACCCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCa    <  1:9711985/140‑1 (MQ=255)

                                                                                                                                   ccGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCa    >  2:8361349/1‑140 (MQ=255)

                                                                                                                                   ccGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCa    >  2:1444530/1‑140 (MQ=255)

                                                                                                                                   ccGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCa    <  2:9756196/140‑1 (MQ=255)

                                                                                                                                     gACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGc  <  1:3522209/140‑1 (MQ=255)

TGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGC  >  NZ_CP025268/441964‑442236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`