Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 1,206,865 (T)6→5 pseudogene (1043/1129 nt) intE ← site‑specific integrase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252681,206,8600T.99.8% 12058.2 / 7.1 2517pseudogene (1048/1129 nt)intEsite‑specific integrase
Reads supporting (aligned to +/- strand):  ref base T (3/2);  new base . (916/1594);  total (919/1598)
Fisher's exact test for biased strand distribution p-value = 3.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2712 total aligned reads displayed.

TTTTAGTTTTTATCATGTGCGGTGAGAAATTCAATGTGGCGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCGCAGGTGAACCCTCTCCCCAGTCAATTCTTGCTAAATCTCTCGCTTTACTAAAATTCATCGTCAATGTAT  >  NZ_CP025268/1206721‑1206997
                                                                                                                                           |                                                                                                                                         
 tttAGTTTTTATCATGTGCGGTGAGAAATTCAATGTGGCGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGtt                                                                                                                                          >  2:10495256/1‑140 (MQ=255)
 tttAGTTTTTATCATGTGCGGTGAGAAATTCAATGTGGCGTTGAGATGCTTAAAGGTTAACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCTGTTTGTTGTTGTGTTTTATGACCAAGTAGtt                                                                                                                                          >  1:6842450/1‑140 (MQ=255)
       ttttATCATGTGCGGTGAGAAATTCAATGTGGCGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGtttttgtg                                                                                                                                    <  2:268762/140‑4 (MQ=255)
       ttttATCATGTGCGGTGAGAAATTCAATGTGGCGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGtttttgtg                                                                                                                                    <  1:4422599/140‑4 (MQ=255)
         ttATCATGTGCGGTGAGAAATTCAATGTGGCGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATACCGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTtgtgtg                                                                                                                                 >  2:36969/1‑140 (MQ=255)
            tCATGTGCGGTGAGAAATTCAATGTGGCGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCt                                                                                                                              <  1:7970130/140‑1 (MQ=255)
            tCATGTGCGGTGAGAAATTCAATGTGGCGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCt                                                                                                                              <  2:4946093/140‑1 (MQ=255)
            tCATGTGCGGTGAGAAATTCAATGTGGCGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCt                                                                                                                              <  2:6781170/140‑1 (MQ=255)
                gtgCGGTGAGAAATTCAATGTGGCGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAAcc                                                                                                                          >  1:10503523/1‑140 (MQ=255)
                gtgCGGTGAGAAATTCAATGTGGCGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTCGTTGTGTTTTATGACCAAGTAGtttttgtg                                                                                                                                    >  1:10001573/1‑128 (MQ=255)
                 tgCGGTGAGAAATTCAATGTGGCGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAAccc                                                                                                                         <  2:6997197/140‑1 (MQ=255)
                 tgCGGTGAGAAATTCAATGTGGCGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAAccc                                                                                                                         <  1:1643486/140‑1 (MQ=255)
                   cGGTGAGAAATTCAATGTGGCGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCt                                                                                                                       <  2:1716290/140‑1 (MQ=255)
                   cGGTGAGAAATTCAATGTGGCGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCt                                                                                                                       <  1:5850646/140‑1 (MQ=255)
                   cGGTGAGAAATTCAATGTGGCGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCt                                                                                                                       <  2:2896428/140‑1 (MQ=255)
                   cGGTGAGAAATTCAATGTGGCGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCt                                                                                                                       <  1:5121160/140‑1 (MQ=255)
                   cGGTGAGAAATTCAATGTGGCGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCt                                                                                                                       <  2:8543112/140‑1 (MQ=255)
                   cGGTGAGAAATTCAATGTGGCGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCt                                                                                                                       <  1:8175069/140‑1 (MQ=255)
                     gTGAGAAATTCAATGTGGCGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCTTCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGt                                                                                                                     <  2:10148695/140‑1 (MQ=255)
                      tGAGTAATTCAAGGTGGCGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCTTGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGtt                                                                                                                    <  2:8145583/140‑1 (MQ=255)
                      tGAGAAATTCAATGTGGCGTTGAGATGCTTAAAGGTTCTCAACGATACTTTGCTACATACTTTACCTCGATCATCATGATAACGATCTGTTTGTTGTTGTGTTTTATGACCAATTAGTTTTTGTGTGTCTAACCCCTGtt                                                                                                                    <  1:464476/140‑1 (MQ=255)
                      tGAGAAATTCAATGTGGCGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGtt                                                                                                                    <  2:1307030/140‑1 (MQ=255)
                      tGAGAAATTCAATGTGGCGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGtt                                                                                                                    <  2:9569114/140‑1 (MQ=255)
                      tGAGAAATTCAATGTGGCGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGtt                                                                                                                    <  1:3624658/140‑1 (MQ=255)
                      tGAGAAATTCAATGTGGCGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGtt                                                                                                                    <  2:4535670/140‑1 (MQ=255)
                      tGAGAAATTCAATGTGGCGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGtt                                                                                                                    <  1:1277912/140‑1 (MQ=255)
                      tGAGAAATTCAATGTGGCGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGtt                                                                                                                    <  1:8553412/140‑1 (MQ=255)
                      tGAGAAATTCAATGTGGCGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGtt                                                                                                                    <  1:1104451/140‑1 (MQ=255)
                      tGAGAAATTCAATGTGGCGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGtt                                                                                                                    <  2:7900540/140‑1 (MQ=255)
                      tGAGAAATTCAATGTGGCGTTGAGATGCTTAAAGGTTCAAAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGtt                                                                                                                    <  2:253624/140‑1 (MQ=255)
                      tGAGAAATTCAATGTGGAGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGtt                                                                                                                    <  2:7517162/140‑1 (MQ=255)
                        agaAATTCAATGTGGCGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCt                                                                                                                  <  2:5882948/140‑1 (MQ=255)
                         gaAATTCAATGTGGCGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCtt                                                                                                                 >  1:3807568/1‑140 (MQ=255)
                           aaTTCAATGTGGCGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCTTta                                                                                                               <  2:6577244/140‑1 (MQ=255)
                             ttCAATGTGGCGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCTTtata                                                                                                             <  1:737417/140‑1 (MQ=255)
                             ttCAATGTGGCGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCTTtata                                                                                                             <  2:7199199/140‑1 (MQ=255)
                                  tgtgGCGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCTTta                                                                                                               >  1:886974/1‑133 (MQ=255)
                                     ggCGTTGAGATGCTCAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGtt                                                                                                     <  1:3323103/140‑1 (MQ=255)
                                      gCGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTc                                                                                                    <  1:6486981/140‑1 (MQ=255)
                                      gCGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTc                                                                                                    <  1:8394359/140‑1 (MQ=255)
                                       cGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCa                                                                                                   >  2:986376/1‑140 (MQ=255)
                                              aTGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCt                                                                                                                       >  1:2901217/1‑113 (MQ=255)
                                                gCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCt                                                                                                                       >  2:4161203/1‑111 (MQ=255)
                                                gCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGAc                                                                                          >  2:2344583/1‑140 (MQ=255)
                                                  ttAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCt                                                                                        <  1:9491598/140‑1 (MQ=255)
                                                      aGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGtt                                                                                                                    <  1:10202332/108‑1 (MQ=255)
                                                      aGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGc                                                                                    <  2:5118882/140‑1 (MQ=255)
                                                      aGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGc                                                                                    >  1:4898768/1‑140 (MQ=255)
                                                         ttCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCa                                                                                 <  2:9203275/140‑1 (MQ=255)
                                                         ttCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCa                                                                                 >  1:2719335/1‑140 (MQ=255)
                                                           cacaACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATg                                                                               >  1:9281021/1‑140 (MQ=255)
                                                           cacaACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATg                                                                               >  1:3471565/1‑140 (MQ=255)
                                                           cacaACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATg                                                                               >  1:4010937/1‑140 (MQ=255)
                                                            acaACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATgg                                                                              <  1:10376075/140‑1 (MQ=255)
                                                            acaACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATgg                                                                              <  1:145079/140‑1 (MQ=255)
                                                              aaCGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGCTCGGTTTGTTGGTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGaa                                                                            <  1:779790/140‑1 (MQ=255)
                                                                  cTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTGTGTTGTTGTGTTTTATGACCAAGTCGTTTTTGTGTGTCTAACCCCTGTTCTTTATAAAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTc                                                                        >  2:4363276/1‑140 (MQ=255)
                                                                   tACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCg                                                                       <  2:8952046/140‑1 (MQ=255)
                                                                        tGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTCACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCGCAGGt                                                                  <  2:1117735/140‑1 (MQ=255)
                                                                          cTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCt                                                                                                                       <  1:6629406/85‑1 (MQ=255)
                                                                          cTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCa                                                                                 >  2:5321414/1‑123 (MQ=255)
                                                                          cTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCGCAGGTGa                                                                <  1:10677317/140‑1 (MQ=255)
                                                                          cTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCGCAGGTGa                                                                <  2:552064/140‑1 (MQ=255)
                                                                             cATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCt                                                                                                                       >  1:6273002/1‑82 (MQ=255)
                                                                             cATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCGCAGGTGAAcc                                                             >  1:5434973/1‑140 (MQ=255)
                                                                               tCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCt                                                                                                                       <  1:2521313/80‑1 (MQ=255)
                                                                               tCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCGCAGGTGAACCct                                                           >  2:1923666/1‑140 (MQ=255)
                                                                               tCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCGCAGGTGAACCct                                                           >  2:3345259/1‑140 (MQ=255)
                                                                               tCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCGCAGGTGAACCct                                                           >  2:7727136/1‑140 (MQ=255)
                                                                               tCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCGCAGGTGAACCct                                                           >  2:737324/1‑140 (MQ=255)
                                                                                  tttACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTc                                                                                                    >  1:5652379/1‑96 (MQ=255)
                                                                                    tACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCt                                                                                                                       >  2:10289505/1‑75 (MQ=255)
                                                                                    tACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCGCAGGTGAACCCTCTccc                                                      <  2:3156880/140‑1 (MQ=255)
                                                                                       cTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCGCAGGTGAACCCTCTCCCCAg                                                   >  1:1861542/1‑140 (MQ=255)
                                                                                        tCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCGCAGGTGAACCCTCTCCCCAGt                                                  <  2:8740600/140‑1 (MQ=255)
                                                                                        tCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCGCAGGTGAACCCTCTCCCCAGt                                                  <  1:9052551/140‑1 (MQ=255)
                                                                                            tcatcaTGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCGCAGGTGAACCCTCTCCCCAGTCAAt                                              <  2:157187/140‑1 (MQ=255)
                                                                                                catGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCGCAGGTGAACCCTCTCCCCAGTCAATTCtt                                          >  2:4681338/1‑140 (MQ=255)
                                                                                                 atGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCGCAGGTGAACCCTCTCCCCAGTCAATTCTTg                                         >  2:9396999/1‑140 (MQ=255)
                                                                                                 atGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCGCAGGTGAACCCTCTCCCCAGTCAATTCTTg                                         >  2:6391593/1‑140 (MQ=255)
                                                                                                   gATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCGCAGGTGAACCCTCTCCCCAGTCAATTCTTGCt                                       <  2:8342637/140‑1 (MQ=255)
                                                                                                        cGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCGCAGGTGAACCCTCTCCCCAGTCAATTCTTGCTAAAtc                                  >  1:701949/1‑140 (MQ=255)
                                                                                                          aTCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCGCAGGTGAACCCTCTCCCCAGTCAATTCTTGCTAAAtctc                                >  1:232864/1‑140 (MQ=255)
                                                                                                          aTCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCGCAGGTGAACCCTCTCCCCAGTCAATTCTTGCTAAAtctc                                >  1:1985608/1‑140 (MQ=255)
                                                                                                          aTCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCTATATCCAGACGTTCATATAAAGACCTTTGCTCATGGAATGTCGCAGGTGAAACCTCACCCCAGTCAATTCTTGCTCAAtctc                                >  2:2096352/1‑140 (MQ=255)
                                                                                                                ttgttgttgTGTTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCGCAGGTGAACCCTCTCCCCAGTCAATTCTTGCTAAATCTCTCGCtt                          <  2:3479666/140‑1 (MQ=255)
                                                                                                                         tgtTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCGCAGGTGAACCCTCTCCCCAGTCAATTCTTGCTa                                      >  2:10708005/1‑119 (MQ=255)
                                                                                                                         tgtTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCGCAGGTGAACCCTCTCCCCAGTCAATTCTTGCTAAATCTCTCGCTTTACTAAAAt                 >  2:38822/1‑140 (MQ=255)
                                                                                                                         tgtTTTATGACCAAGTAGTTTTTGTGTGTCTAACCCCAGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCGCAGGTGAACCCTCTCCCCAGTCAATTCTTGCTAAATCTCTCGCTTTACTAAAAt                 >  1:995888/1‑140 (MQ=255)
                                                                                                                           ttttATGACCAAGTAGTTTTTGTGTGTCTAACCCCt                                                                                                                       <  1:6068664/36‑1 (MQ=255)
                                                                                                                           ttttATGACCAAGTAGTTTTTGTGTGTCTAACCCCt                                                                                                                       >  2:6068664/1‑36 (MQ=255)
                                                                                                                             ttATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCGCAGGTGAACCCTCTCCCCAGTCAATTCTTGCTAAATCTCTCGCTTTACTAAAATTCAt             >  1:5267410/1‑140 (MQ=255)
                                                                                                                             ttATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCGCAGGTGAACCCTCTCCCCAGTCAATTCTTGCTAAATCTCTCGCTTTACTAAAATTCAt             >  1:7231930/1‑140 (MQ=255)
                                                                                                                             ttATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCGCAGGTGAACCCTCTCCCCAGTCAATTCTTGCTAAATCTCTCGCTTTACTAAAATTCAt             <  1:7570178/140‑1 (MQ=255)
                                                                                                                             ttATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCGCAGGTGAACCCTCTCCCCAGTCAATTCTTGCTAAATCTCTCGCTTTACTAAAATTCAt             <  1:422390/140‑1 (MQ=255)
                                                                                                                             ttATGACCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCGCAGGTGAACCCTCTCCCCAGTCAATTCTTGCTAAATCTCTCGCTTTACTAAAATTCAt             <  1:3125399/140‑1 (MQ=255)
                                                                                                                                 gACCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCGCAGGTGAACCCTCTCCCCAGTCAATTCTTGCTAAATCTCTCGCTTTACTAAAATTCATCGTc         <  1:2158283/140‑1 (MQ=255)
                                                                                                                                  aCCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCGCAGGTGAACCCTCTCCCCAGTCAATTCTTGCTAAATCTCTCGCTTTACTAAAATTCATCGTCa        <  1:975993/140‑1 (MQ=255)
                                                                                                                                  aCCAAGTAGTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCGCAGGTGAACCCTCTCCCCAGTCAATTCTTGCTAAATCTCTCGCTTTACTAAAATTCATCGTCa        <  2:5668428/140‑1 (MQ=255)
                                                                                                                                    cAAGTAGTTTTTGTGGGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCGCAGGTGAACCCTCTCCCCATTCAATTCTTGCTAAATCTCTCGCTTTACTAAAATTCATCGTCAAt      <  2:6220512/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                         
TTTTAGTTTTTATCATGTGCGGTGAGAAATTCAATGTGGCGTTGAGATGCTTAAAGGTTCACAACGCTACTTTGCTCCATCCTTTACCTCGATCATCATGATAACGATCGGTTTGTTGTTGTGTTTTATGACCAAGTAGTTTTTTGTGTGTCTAACCCCTGTTCTTTATACAGACGTTCAGATAAAGACCTTTGCTCATGGAATGTCGCAGGTGAACCCTCTCCCCAGTCAATTCTTGCTAAATCTCTCGCTTTACTAAAATTCATCGTCAATGTAT  >  NZ_CP025268/1206721‑1206997

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: