Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 2,997,537 (A)8→7 intergenic (‑140/+83) CXP41_RS15625 ← / ← CXP41_RS24400 response regulator transcription factor/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252682,997,5300A.93.3% 55.5 / ‑2.0 15intergenic (‑133/+90)CXP41_RS15625/CXP41_RS24400response regulator transcription factor/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base . (8/6);  minor base T (1/0);  total (9/6)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGTTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCC  >  NZ_CP025268/2997409‑2997662
                                                                                                                         |                                                                                                                                    
gAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCAt                                                                                                                   >  1:5220255/1‑140 (MQ=255)
 aGTGTAAATGCTTTAACGCATTCATTTATAATCATCACTTCCATTATTATTATAAGCAAAATCCAAAGAATAAAAACATGAAATAATAATGAAATATAATTAAAAATAAAATTTTTGCTTAAAAAAATACAACAGGCAtt                                                                                                                  >  2:3744765/1‑140 (MQ=255)
    ttataTGATTTAACGCATTAATTTATAATCATCACTTCAATTATTATTATAAGAAAAATCAAAAGAATACATTGATGAAATAATAATGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTaaa                                                                                                               <  2:9359192/136‑1 (MQ=255)
    gtAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTaaa                                                                                                               <  2:3405432/140‑1 (MQ=255)
             ttAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATgt                                                                                                      >  2:4754259/1‑139 (MQ=255)
                   cattcattTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAgatgat                                                                                                <  1:2608383/140‑1 (MQ=255)
                                  atcCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAAtt                                                                                 >  2:7181152/1‑140 (MQ=255)
                                                    ataAGCAAAATCCAAAGAATACATTGATGAAATAATAATGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCAt                                                               >  2:7123620/1‑140 (MQ=255)
                                                                               tgaAATAATAATGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTc                                    >  1:5565903/1‑140 (MQ=255)
                                                                                        aatGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGTCt                           >  1:4285867/1‑140 (MQ=255)
                                                                                        aatGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGTCt                           >  1:4286493/1‑140 (MQ=255)
                                                                                              atatAATTAAAAATAAAATTTTTGCGTAAAAAAACACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCaa                     >  1:1569148/1‑140 (MQ=255)
                                                                                                            aaaatTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGTCTTGCCAAAACAGAATTATTCa       <  2:4285867/140‑1 (MQ=255)
                                                                                                            aaaatTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGTCTTGCCAAAACAGAATTATTCa       <  2:4286493/140‑1 (MQ=255)
                                                                                                                 ttttGCTTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCATAACAGAATTATTCATATcc  <  2:2453627/140‑1 (MQ=255)
                                                                                                                         |                                                                                                                                    
GAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGTTATAAGTCCTCAGGGCTTGCCAAAACAGAATTATTCATATCC  >  NZ_CP025268/2997409‑2997662

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: