BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,701,269	+A	intergenic (‑380/+10)	uidA ← / ← uidR	beta‑glucuronidase/DNA‑binding transcriptional regulator UidR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,701,265	1	.	A	91.4%	2402.0 / 189.3	675	intergenic (‑376/+14)	uidA/uidR	beta‑glucuronidase/DNA‑binding transcriptional regulator UidR
Reads supporting (aligned to +/- strand): ref base . (22/36); new base A (271/343); total (296/379)
Fisher's exact test for biased strand distribution p-value = 4.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 691 total aligned reads displayed.

GTATTGGGAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCG‑AA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTAAA  >  NZ_CP025268/1701129‑1701399

gTATTGGGAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCG‑aa‑a                                                                                                                                       >  2:342277/1‑140 (MQ=255)

      ggAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCG‑AA‑a‑a‑‑agaga                                                                                                                              <  2:2470248/140‑1 (MQ=255)

           gATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGaa                                                                                                                           >  1:1871103/1‑140 (MQ=255)

             tAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATc                                                                                                                         >  1:2241330/1‑140 (MQ=255)

              aaaCTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCa                                                                                                                        <  2:263906/140‑1 (MQ=255)

                aCTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAgg                                                                                                                      <  2:4011489/140‑1 (MQ=255)

                 ctctCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGa                                                                                                                     <  1:9580850/140‑1 (MQ=255)

                 ctctCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGa                                                                                                                     <  1:6590116/140‑1 (MQ=255)

                  tctcATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGATACGCGGGCACAGTTATCACCAGCCCGCCG‑AA‑A‑ATGAGAGAGAATCAGGAt                                                                                                                    >  1:8282537/1‑140 (MQ=255)

                  tctcATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGAt                                                                                                                    <  2:2791769/140‑1 (MQ=255)

                  tctcATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGAt                                                                                                                    <  1:9178086/140‑1 (MQ=255)

                  tctcATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGAt                                                                                                                    <  1:4854416/140‑1 (MQ=255)

                  tctcATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGAt                                                                                                                    <  1:5390765/140‑1 (MQ=255)

                  tctcATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGAt                                                                                                                    <  1:6031053/140‑1 (MQ=255)

                  tctcATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGAt                                                                                                                    <  1:7008262/140‑1 (MQ=255)

                  tctcATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGAt                                                                                                                    <  2:1353699/140‑1 (MQ=255)

                  tctcATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGAt                                                                                                                    <  1:7431373/140‑1 (MQ=255)

                  tctcATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGAt                                                                                                                    <  1:8491845/140‑1 (MQ=255)

                  tctcATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGAt                                                                                                                    <  1:8675403/140‑1 (MQ=255)

                  tctcATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGAt                                                                                                                    <  1:10847993/140‑1 (MQ=255)

                  tctcATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGAt                                                                                                                    <  1:114227/140‑1 (MQ=255)

                  tctcATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGAt                                                                                                                    <  1:2669107/140‑1 (MQ=255)

                  tctcATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGAt                                                                                                                    <  2:4298344/140‑1 (MQ=255)

                  tctcATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGAt                                                                                                                    <  2:4748684/140‑1 (MQ=255)

                    tcATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGc                                                                                                                  <  2:7751710/140‑1 (MQ=255)

                    tcATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGc                                                                                                                  <  1:8850627/140‑1 (MQ=255)

                    tcATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGc                                                                                                                  <  2:1706029/140‑1 (MQ=255)

                     cATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCg                                                                                                                 >  2:9875519/1‑140 (MQ=255)

                     cATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCg                                                                                                                 >  1:6263360/1‑140 (MQ=255)

                      aTGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAgg                                                                                                                      >  2:5213734/1‑134 (MQ=255)

                       tGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGt                                                                                                               <  2:3065480/140‑1 (MQ=255)

                       ggACAGATGGCTACAGTGAAAATTTTGGGCGGAATTCTCCGAAGCCGGGAAGGAGAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTCTCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGCTGCGGt                                                                                                               <  2:10253050/139‑1 (MQ=255)

                        gACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACCGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGtt                                                                                                              <  2:4105370/140‑1 (MQ=255)

                          cATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTaa                                                                                                            <  1:309539/140‑1 (MQ=255)

                            tatGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGc                                                                                                                  <  1:10025293/132‑1 (MQ=255)

                            tatGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGa                                                                                                          <  2:6808064/140‑1 (MQ=255)

                            tatGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGa                                                                                                          <  2:3499818/140‑1 (MQ=255)

                            tatGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTGTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGa                                                                                                          <  2:6095518/140‑1 (MQ=255)

                            tatGGCTACAGGGCAGATTTTGGGGGGAAGTCTCGGAAGCCGGGAAGGATAATTTTTGCACAGAGACAGTACGATATGAAACGCGGGCACAGGTATCACCAGCCCGCGGCAC‑T‑G‑GAGCGAGACTCCGGAGGCGGTGAAGa                                                                                                          >  2:33847/1‑140 (MQ=255)

                                    cAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGACGGATAATGTTTGCACCGAGAAATTACGATATGAAACGCGGGCACATTTAGCACCAGCCCGCCG‑AACA‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCa                                                                                                  >  2:4959618/1‑140 (MQ=255)

                                    cAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCa                                                                                                  >  2:7104479/1‑140 (MQ=255)

                                       tGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAatc                                                                                               >  1:8112236/1‑140 (MQ=255)

                                        gAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAatca                                                                                              >  1:794764/1‑140 (MQ=255)

                                         aaTATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAatcat                                                                                             >  1:10403519/1‑140 (MQ=255)

                                           tcttttGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCG‑AA‑ACA‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATcg                                                                                           <  2:1781207/138‑1 (MQ=255)

                                           tatTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATcg                                                                                           <  2:9009753/140‑1 (MQ=255)

                                              tttGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATcgcgc                                                                                        >  1:1270676/1‑140 (MQ=255)

                                                tGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGt                                                                                      <  1:7652710/140‑1 (MQ=255)

                                                tGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGt                                                                                      <  1:9378078/140‑1 (MQ=255)

                                                tGGGGGGAAGTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGt                                                                                      <  2:10714098/140‑1 (MQ=255)

                                                 ggggggAATTCTCCGAAGCCGGGACGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTa                                                                                     >  2:3746701/1‑140 (MQ=255)

                                                       aaTTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCt                                                                               >  2:5140981/1‑140 (MQ=255)

                                                        aTTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTg                                                                              <  2:8798742/140‑1 (MQ=255)

                                                                 aGCCGGGAAGGATAATCTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGAt                                                                                                                    >  2:8359468/1‑93 (MQ=255)

                                                                   ccGGGACGGATAATTTTTGCACAGAGAAATGACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GTGAGAGAATCAGGATGCGGTTAAGATCCCGCCAATCATCGCGCGTAATCCCTGAGCAAacgacg                                                                   >  2:5657667/1‑140 (MQ=255)

                                                                   ccGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAacgacg                                                                   >  1:7801545/1‑140 (MQ=255)

                                                                   ccGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAacgacg                                                                   >  1:10500660/1‑140 (MQ=255)

                                                                        aaGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACCGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCACGATGCGCTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCa                                                              <  2:20609/140‑1 (MQ=255)

                                                                            aTAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATAAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCAcgcg                                                          <  2:4473018/140‑1 (MQ=255)

                                                                              aaTTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGcc                                                        <  2:7563500/140‑1 (MQ=255)

                                                                              aaTTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGcc                                                        <  2:8477830/140‑1 (MQ=255)

                                                                                     gCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCtt                                                 <  2:1871165/140‑1 (MQ=255)

                                                                                      cacaGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTc                                                <  2:10002581/140‑1 (MQ=255)

                                                                                          gagaAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCt                                            >  1:4428832/1‑140 (MQ=255)

                                                                                          gagaAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCt                                            >  1:8948761/1‑140 (MQ=255)

                                                                                          gagaAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCAGCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCt                                            >  2:9217569/1‑140 (MQ=255)

                                                                                           agaAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGAt                                                                                                                    <  2:9650271/67‑1 (MQ=255)

                                                                                           agaAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCtt                                           <  1:5141428/140‑1 (MQ=255)

                                                                                           agaAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCtt                                           >  1:3830839/1‑140 (MQ=255)

                                                                                                ttACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGACTCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGata                                      <  2:6231646/140‑1 (MQ=255)

                                                                                                ttACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGata                                      <  1:3434403/140‑1 (MQ=255)

                                                                                                   cGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCg                                   >  2:1091697/1‑140 (MQ=255)

                                                                                                     atatGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑TAGAGAGACTCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGc                                 <  1:2540242/140‑1 (MQ=255)

                                                                                                     atatGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGNGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGc                                 >  1:5791112/1‑140 (MQ=255)

                                                                                                     atatGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGc                                 <  2:794764/140‑1 (MQ=255)

                                                                                                     atatGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGc                                 >  2:5943275/1‑140 (MQ=255)

                                                                                                     atatGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGc                                 <  1:9875520/140‑1 (MQ=255)

                                                                                                     atatGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGc                                 <  2:5033153/140‑1 (MQ=255)

                                                                                                     atatGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGc                                 >  1:6793252/1‑140 (MQ=255)

                                                                                                          taaCGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTa                            <  1:1613734/139‑1 (MQ=255)

                                                                                                               cgGGCACTGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCAAATCATCGCGCGTAATCCCTGAGCAAACGACGATTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCg                       <  1:1462682/140‑1 (MQ=255)

                                                                                                               cgGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCg                       <  1:4072990/140‑1 (MQ=255)

                                                                                                               cgGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCg                       <  2:5843699/140‑1 (MQ=255)

                                                                                                                gggCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGt                      <  2:1488548/140‑1 (MQ=255)

                                                                                                                 ggCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTa                     >  1:3210049/1‑140 (MQ=255)

                                                                                                                   cacaGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAgg                   <  2:7438476/140‑1 (MQ=255)

                                                                                                                        ttATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGa              <  1:6713614/140‑1 (MQ=255)

                                                                                                                            cACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCca          >  1:4179442/1‑140 (MQ=255)

                                                                                                                             aCCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCcag         <  2:10508977/140‑1 (MQ=255)

                                                                                                                                aGCCCGCCGAAA‑A‑A‑GAGAGAGAATCTGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCCGTACACCGTTGTTCAGATCcagcag      <  2:662245/140‑1 (MQ=255)

                                                                                                                                aGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCcagcag      >  1:5700060/1‑140 (MQ=255)

                                                                                                                                  cccGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAGTCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGc                                 >  1:4651732/1‑111 (MQ=255)

                                                                                                                                  cccGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTa    >  1:10634934/1‑140 (MQ=255)

                                                                                                                                  cccGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTa    >  2:9465656/1‑140 (MQ=255)

                                                                                                                                  cccGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTa    >  1:1023070/1‑140 (MQ=255)

                                                                                                                                  cccGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTa    >  1:2103412/1‑140 (MQ=255)

                                                                                                                                  cccGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTa    >  2:8148812/1‑140 (MQ=255)

                                                                                                                                  cccGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTa    >  1:4999679/1‑140 (MQ=255)

                                                                                                                                  cccGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTa    >  1:5603803/1‑140 (MQ=255)

                                                                                                                                  cccGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTa    >  1:7267900/1‑140 (MQ=255)

GTATTGGGAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCG‑AA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTAAA  >  NZ_CP025268/1701129‑1701399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`