BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,873,836	(T)_5→6	pseudogene (67/1283 nt)	yeaH →	YeaH/YhbH family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,873,831	1	.	T	97.9%	1910.0 / 24.8	515	pseudogene (62/1283 nt)	yeaH	YeaH/YhbH family protein
Reads supporting (aligned to +/- strand): ref base . (8/3); new base T (289/211); total (297/218)
Fisher's exact test for biased strand distribution p-value = 3.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 578 total aligned reads displayed.

CAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTTCATCA  >  NZ_CP025268/1873692‑1873965

     aCAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑tt‑ttt                                                                                                                                   >  1:10333591/1‑140 (MQ=255)

     aCAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑tt‑ttt                                                                                                                                   >  2:2444888/1‑140 (MQ=255)

       aGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑tt‑tttta                                                                                                                                 <  1:4844456/140‑3 (MQ=255)

       aGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑tt‑tttta                                                                                                                                 <  1:5957901/140‑3 (MQ=255)

       aGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑tt‑tttta                                                                                                                                 <  1:9608931/140‑3 (MQ=255)

         cGAACCGTGGGCCTGTGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑tt‑ttttacg                                                                                                                               <  2:10230977/140‑5 (MQ=255)

         cGAACCGTGGGCCTGAGAAGCTGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAACAGCATGGTGAATCGCCAGCG‑tt‑ttttacg                                                                                                                               <  1:7560325/140‑5 (MQ=255)

         cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑tt‑ttttacg                                                                                                                               <  2:8484335/140‑5 (MQ=255)

         cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑tt‑ttttacg                                                                                                                               >  1:4502194/1‑136 (MQ=255)

         cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑tt‑ttttacg                                                                                                                               <  2:5656952/140‑5 (MQ=255)

         cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑tt‑ttttacg                                                                                                                               <  2:4001171/140‑5 (MQ=255)

         cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑tt‑ttttacg                                                                                                                               <  2:7451349/140‑5 (MQ=255)

                tGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTtat                                                                                                                         <  1:2169948/140‑1 (MQ=255)

                tGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTtat                                                                                                                         >  2:10024473/1‑140 (MQ=255)

                     cTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAgc                                                                                                                    <  2:7828047/140‑1 (MQ=255)

                       gagaAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAgcgc                                                                                                                  <  2:4579369/140‑1 (MQ=255)

                             cGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTa                                                                                                            >  1:1904704/1‑140 (MQ=255)

                             cGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTa                                                                                                            >  1:10703241/1‑140 (MQ=255)

                               gCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTaaa                                                                                                          <  2:8282855/140‑1 (MQ=255)

                               gCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTaaa                                                                                                          <  1:7400878/140‑1 (MQ=255)

                               gCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTaaa                                                                                                          <  1:6297586/140‑1 (MQ=255)

                               gCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTATATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATAGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTaaa                                                                                                          >  2:7249218/1‑140 (MQ=255)

                                 aaCACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACa                                                                                                        >  2:2239469/1‑140 (MQ=255)

                                   cacaGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATCAAGCGCAAATTAAACAGt                                                                                                      >  2:3226943/1‑140 (MQ=255)

                                     caGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCg                                                                                                    <  1:8118562/140‑1 (MQ=255)

                                     caGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCg                                                                                                    <  2:2862196/140‑1 (MQ=255)

                                     caGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCg                                                                                                    <  2:3063012/140‑1 (MQ=255)

                                     caGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCg                                                                                                    >  2:6362056/1‑140 (MQ=255)

                                           tAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTcc                                                                                              <  1:10024474/140‑1 (MQ=255)

                                             gCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑tt‑tttta                                                                                                                                 >  2:8596535/1‑100 (MQ=255)

                                               aTACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                          <  2:3656195/140‑1 (MQ=255)

                                               aTACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                          <  1:10553395/140‑1 (MQ=255)

                                                  cAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTCTAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCa                                                                                       >  2:5511338/1‑140 (MQ=255)

                                                  cAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGa                                                                                            <  1:8588323/135‑1 (MQ=255)

                                                      tGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaa                                                                                   >  1:3600695/1‑140 (MQ=255)

                                                       ggCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCAtt                                                                                     >  1:7799884/1‑137 (MQ=255)

                                                       ggCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCAtt                                                                                     <  2:7799883/137‑1 (MQ=255)

                                                       ggCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaat                                                                                  <  1:3865745/140‑1 (MQ=255)

                                                       ggCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaat                                                                                  >  2:7021387/1‑140 (MQ=255)

                                                       ggCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGATATAAAGCGCAAATTAAACAGACGATCTCCCACGCCaaaaat                                                                                  >  2:5932528/1‑135 (MQ=255)

                                                        gCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCACATTAAACAGTCGATCTCCGAGGCCAGtaata                                                                                 >  1:7082285/1‑140 (MQ=255)

                                                        gCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaata                                                                                 >  2:1670360/1‑140 (MQ=255)

                                                        gCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaata                                                                                 >  1:5536340/1‑140 (MQ=255)

                                                          aaaTGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATCAg                                                                               >  2:6614105/1‑140 (MQ=255)

                                                          aaaTGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAg                                                                               <  1:4391437/140‑1 (MQ=255)

                                                             tGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGt                                                                            >  2:8972373/1‑140 (MQ=255)

                                                             tGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGAACTCCGAGGCCATTAATAAGCGt                                                                            >  2:3420019/1‑140 (MQ=255)

                                                               tagtaCGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTc                                                                          >  1:5280786/1‑140 (MQ=255)

                                                               tagtaCGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTc                                                                          >  1:230314/1‑140 (MQ=255)

                                                                      gggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgact                                                                   <  1:1489095/140‑1 (MQ=255)

                                                                       ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAgcgc                                                                                                                  >  1:934889/1‑92 (MQ=255)

                                                                       ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAgc                                                                                                                    >  2:1591338/1‑90 (MQ=255)

                                                                       ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                          >  1:7309219/1‑116 (MQ=255)

                                                                       ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactg                                                                  >  1:9925659/1‑140 (MQ=255)

                                                                       ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactg                                                                  >  1:7943301/1‑140 (MQ=255)

                                                                       ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactg                                                                  >  1:2012304/1‑140 (MQ=255)

                                                                       ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactg                                                                  >  1:5608981/1‑140 (MQ=255)

                                                                        gggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactga                                                                 >  1:2427838/1‑140 (MQ=255)

                                                                         ggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactgac                                                                >  1:8444805/1‑140 (MQ=255)

                                                                         ggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactgac                                                                >  1:4214067/1‑140 (MQ=255)

                                                                           cATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGt                                                              <  1:10385226/140‑1 (MQ=255)

                                                                            atatGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTc                                                             >  2:500042/1‑140 (MQ=255)

                                                                               tGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGAc                                                          <  2:3570632/140‑1 (MQ=255)

                                                                                     ggTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGGCGACAgcggc                                                    >  2:10854152/1‑140 (MQ=255)

                                                                                      gTTTATTGACCGGCGTCTGACCGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggcg                                                   <  2:9840662/140‑1 (MQ=255)

                                                                                      gTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggcg                                                   >  1:5039829/1‑140 (MQ=255)

                                                                                            tGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCg                                             <  2:10463606/140‑1 (MQ=255)

                                                                                            tGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCg                                             >  2:8804189/1‑140 (MQ=255)

                                                                                            tGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCg                                             >  1:6890161/1‑140 (MQ=255)

                                                                                            ccacccccGTCGGAACGGCAAAAACAACACCATGGCGACGCCCCCGCG‑TTAATTACGCCGTTATACAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCg                                             <  2:8019533/133‑1 (MQ=255)

                                                                                                cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATc                                         >  1:1247193/1‑140 (MQ=255)

                                                                                                cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATc                                         >  1:3772337/1‑140 (MQ=255)

                                                                                                cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATc                                         >  1:1682642/1‑140 (MQ=255)

                                                                                                cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATc                                         <  1:6653191/140‑1 (MQ=255)

                                                                                                 ggCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATcc                                        >  1:6462905/1‑140 (MQ=255)

                                                                                                     tCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTc                                    >  1:5888862/1‑140 (MQ=255)

                                                                                                        gAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCa                                 <  1:1634686/140‑1 (MQ=255)

                                                                                                        gAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCa                                 >  1:9059214/1‑140 (MQ=255)

                                                                                                        gAACGGCAAAAACAAAAGCATGGCGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCa                                 >  2:9509888/1‑140 (MQ=255)

                                                                                                         aaCGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCAc                                >  2:1265537/1‑140 (MQ=255)

                                                                                                          aCGGCAAAAACAATAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATTAAGCGCTAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATACGTATCCATTCCCACg                               <  2:527137/140‑1 (MQ=255)

                                                                                                          aCGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACg                               <  2:4085533/140‑1 (MQ=255)

                                                                                                           cGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACgg                              <  2:4781415/140‑1 (MQ=255)

                                                                                                            ggCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTACACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGa                             <  2:4319663/140‑1 (MQ=255)

                                                                                                            ggCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGGTCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGa                             <  2:3777455/140‑1 (MQ=255)

                                                                                                            ggCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGa                             <  1:8845954/140‑1 (MQ=255)

                                                                                                            ggCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGA‑CTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGaa                            <  1:6837683/140‑1 (MQ=255)

                                                                                                             gCAAAAACAAAAGCATGTTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGaa                            >  2:190647/1‑140 (MQ=255)

                                                                                                             gCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGaa                            >  1:2719765/1‑140 (MQ=255)

                                                                                                              cAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTaaa                                                                                                          <  2:6081607/61‑1 (MQ=255)

                                                                                                               aaaaaCAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGa                          <  1:2980858/140‑1 (MQ=255)

                                                                                                                       aaGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCg                  <  1:7233063/140‑1 (MQ=255)

                                                                                                                        aGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGa                 <  2:2633799/140‑1 (MQ=255)

                                                                                                                          cATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAAc               <  2:6883548/140‑1 (MQ=255)

                                                                                                                           aTGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAAcc              <  1:3335336/140‑1 (MQ=255)

                                                                                                                           aTGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAAcc              <  2:7693704/140‑1 (MQ=255)

                                                                                                                              gTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGAt           <  1:2605213/140‑1 (MQ=255)

                                                                                                                                gAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGt         <  1:3206484/140‑1 (MQ=255)

                                                                                                                                gAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGt         <  1:5232184/140‑1 (MQ=255)

                                                                                                                                      ccAGCGTTT‑TTTACGCCGGTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTtcatc   <  1:4137704/140‑1 (MQ=255)

CAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTTCATCA  >  NZ_CP025268/1873692‑1873965

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`