Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252682,233,2330GC22.3% 238.9 / 32.5 121intergenic (+133/+240)yohP/dusCsmall membrane protein YohP/tRNA dihydrouridine(16) synthase DusC
Reads supporting (aligned to +/- strand):  ref base G (50/44);  new base C (27/0);  total (77/44)
Fisher's exact test for biased strand distribution p-value = 7.06e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 131 total aligned reads displayed.

ATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCC  >  NZ_CP025268/2233094‑2233372
                                                                                                                                            |                                                                                                                                            
atTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATcgccgccg                                                                                                                                              >  1:8226112/1‑140 (MQ=255)
atTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATcgccgccg                                                                                                                                              <  2:6266380/140‑1 (MQ=255)
  tttAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCgccgccgcc                                                                                                                                            <  2:7446988/140‑1 (MQ=255)
              taatGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCAccccc                                                                                                                                <  2:5500518/140‑1 (MQ=255)
                 ggTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAAACACGGGCGCACGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCggg                                                                                                                             <  1:3928286/139‑1 (MQ=255)
                     atcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCCAAAGCCCCCCCCCCCAGCACCCCCCGGctct                                                                                                                         >  1:9913442/1‑140 (MQ=37)
                      tccggTCCGAAAATAACGAGAATCTTTCCGGCTCTCATCCTGTTGCGCTCCTGTCATGTGCAGTGCTTCCTAGTATCACTGGCGCGAGGAGCGC‑GCAGGGGGCGGGCCCTCGCCGCCGCCCCCGGCACCCCCGGGCGCTg                                                                                                                        >  2:3678740/4‑140 (MQ=21)
                      tcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GGGGGGGGGGGGCAAAAGCCCCCCCCCCCCCGACCCCCCGGcgcag                                                                                                                        >  1:597375/1‑136 (MQ=255)
                      tcaGGTCCGAAAAAAACGAGAATATTTCAGTCTCTCTTCGTGTTGCGCTCCTGTCATGAGCATTGCTTAATATAATCACTGGAGCAAGGAGCGC‑GCAGGGGGGTGCCAATGGCCCGCGGCCCCTGCACACCCGGGCGCTg                                                                                                                        >  2:6756223/1‑140 (MQ=25)
                          gTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGGGGCCAAAAGCCGCCGCCCCCAGCACCCCCCGGCGCAGGCGa                                                                                                                    >  2:1136370/1‑140 (MQ=25)
                          gTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAACGCCGCCGCCCCCAGCACCCCCCGGCGCTGGCGa                                                                                                                    >  1:4583319/1‑140 (MQ=255)
                             cGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACa                                                                                                                >  2:9529544/1‑140 (MQ=255)
                             cGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCAGCACCCCCGGGCTCAGGCGA‑ACa                                                                                                                >  2:3707303/1‑140 (MQ=255)
                               aaaaTAACGAGAATATTTCAGTCTCTCCTCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCAGCACCCCCGGGCTCTGGCGc‑acaca                                                                                                              >  2:8851865/1‑138 (MQ=255)
                               aaaaTAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACaaa                                                                                                              >  2:8172508/1‑140 (MQ=255)
                                    aaCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GGCGGGGGCGGGCAAAAGCCGCCCCCCCCCGCACCCCCCGGCGCTGGCGC‑ACAAAAAcgc                                                                                                         >  1:7082540/1‑140 (MQ=25)
                                    aaCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATagc                                                                                                         >  2:10607357/1‑140 (MQ=255)
                                     aCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGCGGGGGGGGGCCAA‑CCCCCCCCCCCCCAACCCCCCCCCCCCCTGGCGG‑ACaaaaaaacc                                                                                                        >  1:5270288/1‑135 (MQ=255)
                                     aCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGGGGCCAATGGCCCCCGCCCCCACCACCCCCCGGGTCTGGCGA‑ACaaaaaagcc                                                                                                        >  2:1306273/1‑135 (MQ=21)
                                     aCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATcgcc                                                                                                        <  2:4020717/140‑1 (MQ=255)
                                     aCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAACGCCGCCGCCCCCAGCACCCCCCGGCTCAGGCGA‑ACAAAATagcc                                                                                                        >  2:5620300/1‑140 (MQ=21)
                                            tatTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGCGGCCCAAAGCCCCCCCCCCCACCACCCCCCGGCTCTGGCGA‑ACCAAATAGCCGCAgcgc                                                                                                 >  1:8916100/1‑140 (MQ=21)
                                            tatTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATAGCCGCCCCCCCCAGCACCCCCGGGCTCTGGCGA‑AAAAAATCGCCGCGgcgc                                                                                                 >  2:1672862/1‑140 (MQ=21)
                                                    tctctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCCCCGCCCCCAGCACCCCCGGGCTCAGGCGA‑ACAAAATCGCCGCAGCGCGGTGccca                                                                                         >  2:8803646/1‑139 (MQ=255)
                                                      tctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCACGGGGCGGCCAAAAGCCCCCGCCCCCAGCACCCCCCGGCACAGGCGA‑AAAAAAAAGCCCCAGCGCCGTGCCCACg                                                                                       >  2:3503441/1‑140 (MQ=18)
                                                        tcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGCGGCCCAAAACCCCCCCCCCCACCACCCCCCGGCTCAGGCGA‑AAAAAAAAACCCCAGCGCGGTGCCCACGGc                                                                                     >  1:10845147/1‑140 (MQ=16)
                                                        tcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGGGGCCCAACGCCCCCCCCCCCAGCACCCCCCCGCGCTTGCGACACAAAA‑AACCCCAGCGCGGGGCCCTCCGc                                                                                     >  1:9550354/1‑140 (MQ=16)
                                                         cATCCTGTTGCGCTCCTGTCATGTGCATTGCTGCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCCATCGGCGCCGCCCCCAGCACCCACGGGCTCTGGCGT‑ACCAAATCGGCGCTGCGGGGGGGCCTggggg                                                                                    >  2:9123069/1‑138 (MQ=17)
                                                         cATCCTGTGGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGCGGCCAATCGCAGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑AACAACTCGCCGCTGCGCGGTGCCCTCGGCt                                                                                    >  2:6569557/1‑140 (MQ=18)
                                                                ttGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GGATGGGGGCGCCAAAACCCCCCCCCCCCACCCCCCCCCCGCGCAGGCGT‑ACAAAAAAGCCGCGGCGCGGGGGCCACGGCATATacca                                                                             >  2:103227/1‑139 (MQ=255)
                                                                ttGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGGGGCCAAAACCCCCCCCCCCCAGCAACCCCCGGCACAGGCGA‑ACAAAAACCCCCCGGCGCCGTGGCCACCGCGTATacca                                                                             >  1:4723040/1‑139 (MQ=12)
                                                                ttGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATAGCCGCCGCCCCCAGCACCCCCCCGCTCTGGCGA‑ACAAAATAGCCGCAGCGCGGTGCCCACGGCGTATccca                                                                             >  2:6785481/1‑139 (MQ=18)
                                                                ttGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAAACCCCCCCCCCCCAGCACCCCCCGGCACAGGCGA‑ACAAAAAAGCCCCAGCGCGGTGCCCACGGCGTATacca                                                                             >  2:1156827/1‑139 (MQ=18)
                                                                  gcgcTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCACCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAACCCtga                                                                           >  2:9382461/1‑138 (MQ=255)
                                                                  gcgcTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATAGCCGCCGCCCCCAGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCGTATCCCATa                                                                           >  2:9068817/1‑140 (MQ=255)
                                                                   cgcTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTAc                                                                          <  1:5393948/140‑1 (MQ=255)
                                                                     cTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCATGGGGCGGCCCAACGCCCCCGCCCCCAGCACCCCCCGGCACAGTCGA‑ACAAAATACCCCCGGCGCGGTGCCCACCGCGTATcccgaaccg                                                                        >  2:291913/1‑134 (MQ=16)
                                                                     cTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGTGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCAGGCGA‑ACAAAATCGCCGCTGCTCGGTGCCCACTGCTTATAACTTACgg                                                                        >  2:4788190/1‑140 (MQ=18)
                                                                     cTCATGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCCCCCCCAGCACCCCCGGGCACAGGCGA‑ACAAAAACGCCGCAGCGCGCTGCCCTCGGCATATAACATACgg                                                                        >  2:4787928/1‑140 (MQ=17)
                                                                       ccTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCCATCGCCGCCGCCCCCACCACCCCCCGGCTCTGGCGA‑CCAAAAACGCCGCAGCCCCGTGCCCGCGGCATATCCCATACGGCt                                                                      >  2:10314666/1‑140 (MQ=17)
                                                                        cTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCCATCGCCGCCGCCCCCAGCACCCCCCGGCTCAGGCGA‑ACAAAAACGCCGCGGCGCGGTGCCCTCGGCATATCCCATACGGCTa                                                                     >  2:7124645/1‑140 (MQ=17)
                                                                         tGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTAc                                                                    <  1:1891231/140‑1 (MQ=255)
                                                                              tgtgCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAGCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGt                                                               <  1:10314667/140‑1 (MQ=255)
                                                                                tgCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGCGGCCAAACGCCGCCCCCCCCAGCACCCCCCGGCTCTGGCGA‑ACAAAAACGCCGCTGCGCGGTGCCCACGGCATATCCCTTACGGCTACCGCGTCg                                                             >  2:9556051/1‑140 (MQ=14)
                                                                                tgCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGGGGCCCATCCCCCCCCCCCCCGGCACCCCCCGGCTCTTGCGA‑ACAAAAAACCCCCAGCGCGGTGCCCACCGCGGAACCCATACCGCTAACCGGTCg                                                             >  2:610776/1‑140 (MQ=9)
                                                                                tgCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCAGCACCCCCCGGCTCTGGCGA‑ACAAAAACCCCGCAGCGCGGAGCCCTCCGCATATCCCTTACGGCTACCGGGTCg                                                             >  2:9715988/1‑140 (MQ=14)
                                                                                tgCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAAAGCCGCCCCCCCCAGCACCCCCCGGCTCAGGCGG‑ACAAAAACGCCGCAGAGCGGTGGCCGCGGCGTATACCCTACGGCTACCGGGGCg                                                             >  2:3084049/1‑140 (MQ=14)
                                                                                  cATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCggg                                                           <  1:7918104/140‑1 (MQ=255)
                                                                                  cATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCggg                                                           <  1:1530031/140‑1 (MQ=255)
                                                                                   aTTGCTTCATATAATCCCTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGgc                                                          <  2:2241384/140‑1 (MQ=255)
                                                                                    ttGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGgcg                                                         <  1:3191903/140‑1 (MQ=255)
                                                                                     tGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGCGGCCCAACCCCCCCCCCCCCCCCACCCCCCGGCACTGGCCA‑CCAAAAACCCCCCAGCGCGGGGCCCGCCGCGTATCCCATAAGGCTACCCGG‑CGGTcgcg                                                       >  2:1338264/1‑140 (MQ=9)
                                                                                     tGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCCAAACCCCCCCCCCCCAGCAACCCCCGGCTCTTGCGC‑AAAAAAAAACCGCAGCGCGGGGCCCCCCGCATAAGCCATAAGGCAAACCCGTCGGgcgc                                                        >  1:6514867/1‑140 (MQ=9)
                                                                                     tGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCCCCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCGCGGCTTATCCCTTACGGCGACCGGGTCGGgcgc                                                        >  2:7169724/1‑140 (MQ=255)
                                                                                     tGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAACGCCGCCGCCCCCTGCCCCCCCCGGCTCTGGCGA‑ACAAAATAGCCGCAGCGCGGGGCCCACGGCGTATCCCAAACGGCTACCCGGTCGGgcgc                                                        >  2:6223627/1‑140 (MQ=14)
                                                                                     tGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAACGCCGCCGCCCCCAGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCCCTGCGCGGTGCCCACGGCTTATCCCATACGGCTACCCGGTCGGgcgc                                                        >  2:5667727/1‑140 (MQ=14)
                                                                                     tGCTTCAGATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGGCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑CCAAAATCGCCGCTGCGCGGTGCCCACGGCTTATCCCTTACGGCTCCCCGGTCGGgcgc                                                        >  2:9799124/1‑140 (MQ=14)
                                                                                            tataATCCCTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTaa                                                 <  1:6785482/140‑1 (MQ=37)
                                                                                            tataATCACTGGCGCAAGGAGCGC‑GGCGGGGGCGGCCAAAAGCCGCCCCCCCCAGCACCCCCCGGCTCTGGGGC‑ACAAAATAGCCGCAGCGCGGTGCCCACGGCGTATACCATAAGGCGACCCGGGGGGGCGCGAGGGaa                                                 >  2:4394736/1‑140 (MQ=2)
                                                                                            tataATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCAGCCCCCTGCACACCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTAACGGGTCGGGCGCGAGGTaa                                                 <  1:624283/140‑1 (MQ=255)
                                                                                            tataATCACTGGCGCAAGGAGCGC‑GACAGTGGCGGGCAAACTCCCCCCCCCCCATCACCCCCGGGCTCTGGCGA‑AAAAAAAAGCCCGAGCGCGGTTGCCTCGGCTGTTCCCATACGGCTACACGGTCGGGCGCGAGGTaa                                                 >  2:8428590/1‑140 (MQ=2)
                                                                                              taATCCCTGGCGCAAGGAGCGC‑GCCGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACa                                               <  1:1156827/140‑1 (MQ=21)
                                                                                              taATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACa                                               <  2:6514866/140‑1 (MQ=35)
                                                                                              taATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACa                                               <  1:8469085/140‑1 (MQ=35)
                                                                                              taATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACa                                               <  2:6853107/140‑1 (MQ=35)
                                                                                              taATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACa                                               <  1:507117/140‑1 (MQ=35)
                                                                                               aaTCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACAt                                              <  2:3778388/140‑1 (MQ=34)
                                                                                               aaTCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACAt                                              <  1:8693785/140‑1 (MQ=34)
                                                                                                 tCACTGGCGCAAGGAGCGC‑GGCGGGGGCGGCCAAACGCCGCCCCCCCCAGCACCCCCCGGCTCTGGCGC‑ACAAAAAAGCCCCCGCGCCGGGCCCCCCGCCTATACCCAACCGCTAACCGGGCGGGCGCCAGGGAACAAcc                                            >  2:8469084/1‑140 (MQ=255)
                                                                                                 tCACTGGCGCAAGGAGCGC‑GCCGGGGGCGGCCAATCGCCCCCCCCCCCAGCCCCCCCCGGCGCTGGCGA‑ACAAAATCCCCCCGGCGCGGTGCCCACGGCGTATACCATACGGCTACCCGGGCGGGCGCGCGGGAACAtac                                            >  2:3653327/1‑138 (MQ=2)
                                                                                                 tCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCCAACGCCGCCGCCCCCAGCACCCCCGGGCTCTGGCGA‑ACAAAATAGCCGCGGCGCGGTGCCCACGGCTTATACCATAACGCTACCCGGTCGGGCGCGAGGTAACAAcc                                            >  2:808809/1‑140 (MQ=11)
                                                                                                 tCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGGCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGGAACagac                                            >  2:9080420/1‑137 (MQ=16)
                                                                                                 tCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCAGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTAAGGCTACCGGGTCGGGCGCGAGGGAACATcc                                            >  2:6449900/1‑140 (MQ=16)
                                                                                                 tCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAAAGCCCCCCCCCCCAGCACCCCCCCGCTCAGGCGA‑AAAAAAAAGCCCCAGCGCGGTGCCCACGGCGTATACCATAAGGCTACCCGGGCGGGCGCGAGGTaaaaacc                                            >  1:9991258/1‑135 (MQ=2)
                                                                                                    cTGGCGCAAGGAGCGC‑GGAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCCCCCCTGCGCGGTGGCCGCGGCGTATCCCTTACGGCTACCCGGTCGGGCGCGTGGTAACCTcccag                                         >  2:4528095/1‑138 (MQ=11)
                                                                                                      ggCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTa                                       <  2:9926337/140‑1 (MQ=32)
                                                                                                               aGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGcc                              <  1:8019168/140‑1 (MQ=32)
                                                                                                                 cgc‑gcAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCt                            <  1:1672862/140‑1 (MQ=32)
                                                                                                                 cgc‑gcAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCt                            <  1:5518519/140‑1 (MQ=32)
                                                                                                                 cgc‑gcAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCt                            <  1:5620300/140‑1 (MQ=32)
                                                                                                                      cAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGc                        <  1:9529545/140‑1 (MQ=32)
                                                                                                                      cAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGc                        <  1:8851866/140‑1 (MQ=32)
                                                                                                                      cAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGc                        <  2:1916741/140‑1 (MQ=32)
                                                                                                                      cAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGc                        <  2:3960937/140‑1 (MQ=32)
                                                                                                                      cAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGc                        <  2:4843444/140‑1 (MQ=32)
                                                                                                                       aGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGcc                       <  2:4166121/140‑1 (MQ=32)
                                                                                                                         ggggCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCca                     <  1:7130984/140‑1 (MQ=32)
                                                                                                                           ggcggcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCcaca                   <  2:7750260/140‑1 (MQ=32)
                                                                                                                           ggcggcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCcaca                   <  2:1345544/140‑1 (MQ=32)
                                                                                                                           ggcggcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCcaca                   <  2:2875404/140‑1 (MQ=32)
                                                                                                                           ggcggcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCcaca                   <  1:6182145/140‑1 (MQ=32)
                                                                                                                               gcCAATCGCCGCCGCCCCCAGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCGCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCAGTAAAACGCGCCCTCAGCCCACATCCa               >  1:7695988/1‑140 (MQ=12)
                                                                                                                                ccAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACCTCCAt              >  1:10446240/1‑140 (MQ=17)
                                                                                                                                   aTCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAATTCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAGCATCCCTGTAAATCGCGCCCTCAGCCCACATCCAtgtg           >  2:6121666/1‑140 (MQ=12)
                                                                                                                                   aTCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAtgtg           >  2:7854414/1‑140 (MQ=32)
                                                                                                                                   aTCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAtgtg           >  1:6201883/1‑140 (MQ=32)
                                                                                                                                   aTCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAtgtg           >  1:3910344/1‑140 (MQ=32)
                                                                                                                                     cgccgccgcCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTggg         >  2:240547/1‑140 (MQ=32)
                                                                                                                                     cgccgccgcCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGGAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTggg         <  2:607627/140‑1 (MQ=17)
                                                                                                                                          ccgccCCCTGCACCCCCGGGCTCTTGCGA‑ACCAATTCGCACCTGCGCGGAGCCCGCGGCTGCTCCCGTGCGTCTACCGCGACGGTCGCGCGGACGCATCCCTGTAAAACGCGCCCTCAGCCCCCCGCCAGGTGGGcagac    >  2:8784479/1‑136 (MQ=2)
                                                                                                                                            |                                                                                                                                            
ATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCC  >  NZ_CP025268/2233094‑2233372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑