Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252682,233,2390TA36.7% 168.3 / 51.2 134intergenic (+139/+234)yohP/dusCsmall membrane protein YohP/tRNA dihydrouridine(16) synthase DusC
Reads supporting (aligned to +/- strand):  ref base T (30/51);  new base A (47/0);  total (83/51)
Fisher's exact test for biased strand distribution p-value = 8.69e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 136 total aligned reads displayed.

TAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTT  >  NZ_CP025268/2233108‑2233378
                                                                                                                                    |                                                                                                                                            
taatGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCAccccc                                                                                                                                      <  2:5500518/140‑1 (MQ=255)
   ggTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAAACACGGGCGCACGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCggg                                                                                                                                   <  1:3928286/139‑1 (MQ=255)
        tcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GGGGGGGGGGGGCAAAAGCCCCCCCCCCCCCGACCCCCCGGcgcag                                                                                                                              >  1:597375/1‑136 (MQ=255)
               cGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCAGCACCCCCGGGCTCAGGCGA‑ACa                                                                                                                      >  2:3707303/1‑140 (MQ=255)
               cGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAACGCCGCCGCCCCCAGCACCCCCCGGCGCTGGCGA‑ACa                                                                                                                      >  2:3706139/1‑140 (MQ=255)
                 aaaaTAACGAGAATATTTCAGTCTCTCCTCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCAGCACCCCCGGGCTCTGGCGc‑acaca                                                                                                                    >  2:8851865/1‑138 (MQ=255)
                 aaaaTAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACaaa                                                                                                                    >  2:8172508/1‑140 (MQ=255)
                      aaCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GGCGGGGGCGGGCAAAAGCCGCCCCCCCCCGCACCCCCCGGCGCTGGCGC‑ACAAAAAcgc                                                                                                               >  1:7082540/1‑140 (MQ=25)
                      aaCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATcgc                                                                                                               >  2:7424523/1‑140 (MQ=255)
                      aaCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATagc                                                                                                               >  2:10607357/1‑140 (MQ=255)
                       aCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGCGGGGGGGGGCCAA‑CCCCCCCCCCCCCAACCCCCCCCCCCCCTGGCGG‑ACaaaaaaacc                                                                                                              >  1:5270288/1‑135 (MQ=255)
                       aCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGGGGCCAATGGCCCCCGCCCCCACCACCCCCCGGGTCTGGCGA‑ACaaaaaagcc                                                                                                              >  2:1306273/1‑135 (MQ=21)
                       aCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATcgcc                                                                                                              <  2:4020717/140‑1 (MQ=255)
                       aCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAACGCCGCCGCCCCCAGCACCCCCCGGCTCAGGCGA‑ACAAAATagcc                                                                                                              >  2:5620300/1‑140 (MQ=21)
                              tatTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGCGGCCCAAAGCCCCCCCCCCCACCACCCCCCGGCTCTGGCGA‑ACCAAATAGCCGCAgcgc                                                                                                       >  1:8916100/1‑140 (MQ=21)
                              tatTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATAGCCGCCCCCCCCAGCACCCCCGGGCTCTGGCGA‑AAAAAATCGCCGCGgcgc                                                                                                       >  2:1672862/1‑140 (MQ=21)
                                        tctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GGAGGGGGCGGCCCATAGCCCCCGCCCCCAGAACCCCCCGGCACTTGCGA‑ACAAAATCGCCGCGGCGCGGGGCCcgcg                                                                                             >  1:5680480/1‑140 (MQ=18)
                                        tctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCACGGGGCGGCCAAAAGCCCCCGCCCCCAGCACCCCCCGGCACAGGCGA‑AAAAAAAAGCCCCAGCGCCGTGCCCACg                                                                                             >  2:3503441/1‑140 (MQ=18)
                                          tcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGGGGCCCAACGCCCCCCCCCCCAGCACCCCCCCGCGCTTGCGACACAAAA‑AACCCCAGCGCGGGGCCCTCCGc                                                                                           >  1:9550354/1‑140 (MQ=16)
                                           cATCCTGTTGCGCTCCTGTCATGTGCATTGCTGCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCCATCGGCGCCGCCCCCAGCACCCACGGGCTCTGGCGT‑ACCAAATCGGCGCTGCGGGGGGGCCTggggg                                                                                          >  2:9123069/1‑138 (MQ=17)
                                           cATCCTGTGGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGCGGCCAATCGCAGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑AACAACTCGCCGCTGCGCGGTGCCCTCGGCt                                                                                          >  2:6569557/1‑140 (MQ=18)
                                             cccTGTTGCGCTCCTGTCAATTTCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGAACCCCCGGGCTATGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTa                                                                                        <  2:10500733/139‑1 (MQ=18)
                                                  ttGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GGATGGGGGCGCCAAAACCCCCCCCCCCCACCCCCCCCCCGCGCAGGCGT‑ACAAAAAAGCCGCGGCGCGGGGGCCACGGCATATacca                                                                                   >  2:103227/1‑139 (MQ=255)
                                                  ttGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGGGGCCAAAACCCCCCCCCCCCAGCAACCCCCGGCACAGGCGA‑ACAAAAACCCCCCGGCGCCGTGGCCACCGCGTATacca                                                                                   >  1:4723040/1‑139 (MQ=12)
                                                  ttGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATAGCCGCCGCCCCCAGCACCCCCCCGCTCTGGCGA‑ACAAAATAGCCGCAGCGCGGTGCCCACGGCGTATccca                                                                                   >  2:6785481/1‑139 (MQ=18)
                                                  ttGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAAAGCCGCCCCCCCCAGCACCCCCCGGCGCAGGCGA‑ACAAAAACGCCCCAGCGCGGTGCCCACCGCTTATccca                                                                                   >  1:9926338/1‑139 (MQ=18)
                                                  ttGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAAACCCCCCCCCCCCAGCACCCCCCGGCACAGGCGA‑ACAAAAAAGCCCCAGCGCGGTGCCCACGGCGTATacca                                                                                   >  2:1156827/1‑139 (MQ=18)
                                                    gcgcTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTa                                                                                 <  2:2621875/140‑1 (MQ=255)
                                                    gcgcTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCACCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAACCCtga                                                                                 >  2:9382461/1‑138 (MQ=255)
                                                    gcgcTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATAGCCGCCGCCCCCAGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCGTATCCCATa                                                                                 >  2:9068817/1‑140 (MQ=255)
                                                     cgcTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTAc                                                                                <  1:5393948/140‑1 (MQ=255)
                                                      gcTCCTGTCTTGTGCATTGGTTCAGATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCCGCGAATCCCCCCGGCCCCCTGGCCCCCCGGGCTCTGGGGC‑ACCAAAACGCCCCAGAGCGGTGGCCTCGGCTTAAACCATACg                                                                               >  2:9693655/1‑140 (MQ=12)
                                                      gcTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGCCGCCACACCCCCCCGCCCCCAGCACCCCCCGGCCCAGGCGA‑ACAAAAAACCCGCGGCGCGGGGCCCAAGGCATATACCATaag                                                                               >  2:5285461/1‑138 (MQ=12)
                                                       cTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCATGGGGCGGCCCAACGCCCCCGCCCCCAGCACCCCCCGGCACAGTCGA‑ACAAAATACCCCCGGCGCGGTGCCCACCGCGTATcccgaaccg                                                                              >  2:291913/1‑134 (MQ=16)
                                                       cTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGTGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCAGGCGA‑ACAAAATCGCCGCTGCTCGGTGCCCACTGCTTATAACTTACgg                                                                              >  2:4788190/1‑140 (MQ=18)
                                                       cTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAACACCCCCCCCCCCAGCACCCCCCGGCACTTGCCA‑ACAAAAACGCCGCGGCCCGGGGGCCACGGCGTATACCATaacg                                                                              >  2:4033319/1‑137 (MQ=12)
                                                       cTCATGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCCCCCCCAGCACCCCCGGGCACAGGCGA‑ACAAAAACGCCGCAGCGCGCTGCCCTCGGCATATAACATACgg                                                                              >  2:4787928/1‑140 (MQ=17)
                                                           tGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTAc                                                                          <  1:1891231/140‑1 (MQ=255)
                                                               aTGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCCCCGCCCCCAGCAACCCCCGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCATACGGCTaaccgg                                                                      >  2:6182145/1‑135 (MQ=17)
                                                                tgtgCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAGCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGt                                                                     <  1:10314667/140‑1 (MQ=255)
                                                                  tgCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGCGGCCAAACGCCGCCCCCCCCAGCACCCCCCGGCTCTGGCGA‑ACAAAAACGCCGCTGCGCGGTGCCCACGGCATATCCCTTACGGCTACCGCGTCg                                                                   >  2:9556051/1‑140 (MQ=14)
                                                                  tgCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGGGGCCCATCCCCCCCCCCCCCGGCACCCCCCGGCTCTTGCGA‑ACAAAAAACCCCCAGCGCGGTGCCCACCGCGGAACCCATACCGCTAACCGGTCg                                                                   >  2:610776/1‑140 (MQ=9)
                                                                  tgCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCg                                                                   >  2:9065514/1‑140 (MQ=255)
                                                                    cATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCggg                                                                 <  1:7918104/140‑1 (MQ=255)
                                                                    cATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCggg                                                                 <  2:3202409/140‑1 (MQ=255)
                                                                     aTTGCTTCATATAATCCCTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGgc                                                                <  2:2241384/140‑1 (MQ=255)
                                                                       tGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCCAAACCCCCCCCCCCCAGCAACCCCCGGCTCTTGCGC‑AAAAAAAAACCGCAGCGCGGGGCCCCCCGCATAAGCCATAAGGCAAACCCGTCGGgcgc                                                              >  1:6514867/1‑140 (MQ=9)
                                                                       tGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCCCCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCGCGGCTTATCCCTTACGGCGACCGGGTCGGgcgc                                                              >  2:7169724/1‑140 (MQ=255)
                                                                       tGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAACGCCGCCGCCCCCTGCCCCCCCCGGCTCTGGCGA‑ACAAAATAGCCGCAGCGCGGGGCCCACGGCGTATCCCAAACGGCTACCCGGTCGGgcgc                                                              >  2:6223627/1‑140 (MQ=14)
                                                                       tGCTTCAGATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGGCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑CCAAAATCGCCGCTGCGCGGTGCCCACGGCTTATCCCTTACGGCTCCCCGGTCGGgcgc                                                              >  2:9799124/1‑140 (MQ=14)
                                                                        gCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGcgcg                                                             <  1:4394736/140‑1 (MQ=255)
                                                                          ttCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCAGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCACGGCGTATCCCATACGGCAACCCGGTCGGGCGCGAg                                                           >  2:6543419/1‑140 (MQ=14)
                                                                              tataATCACTGGCGCAAGGAGCGC‑GGCGGGGGCGGCCAAAAGCCGCCCCCCCCAGCACCCCCCGGCTCTGGGGC‑ACAAAATAGCCGCAGCGCGGTGCCCACGGCGTATACCATAAGGCGACCCGGGGGGGCGCGAGGGaa                                                       >  2:4394736/1‑140 (MQ=2)
                                                                              tataATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCAGCCCCCTGCACACCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTAACGGGTCGGGCGCGAGGTaa                                                       <  1:624283/140‑1 (MQ=255)
                                                                              tataATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAAAGCCGCCGCCCCCAGCACCCCCCGGCTCTGGCGA‑ACCAAATCGCCCCGGCGCGGTGCCCGCGGCTGATACCGTAAGGCTACCCGGTCGGGCGCGAGGTaa                                                       >  2:8693784/1‑140 (MQ=11)
                                                                              tataATCACTGGCGCAAGGAGCGC‑GACAGTGGCGGGCAAACTCCCCCCCCCCCATCACCCCCGGGCTCTGGCGA‑AAAAAAAAGCCCGAGCGCGGTTGCCTCGGCTGTTCCCATACGGCTACACGGTCGGGCGCGAGGTaa                                                       >  2:8428590/1‑140 (MQ=2)
                                                                                taATCCCTGGCGCAAGGAGCGC‑GCCGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACa                                                     <  1:1156827/140‑1 (MQ=21)
                                                                                taATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACa                                                     <  2:6514866/140‑1 (MQ=35)
                                                                                taATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACa                                                     <  2:6853107/140‑1 (MQ=35)
                                                                                taATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACa                                                     <  1:507117/140‑1 (MQ=35)
                                                                                taATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACa                                                     <  1:8469085/140‑1 (MQ=35)
                                                                                 aaTCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACAt                                                    <  2:3778388/140‑1 (MQ=34)
                                                                                 aaTCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACAt                                                    <  1:8693785/140‑1 (MQ=34)
                                                                                   tCACTGGCGCAAGGAGCGC‑GCCGGGGGCGGCCAATCGCCCCCCCCCCCAGCCCCCCCCGGCGCTGGCGA‑ACAAAATCCCCCCGGCGCGGTGCCCACGGCGTATACCATACGGCTACCCGGGCGGGCGCGCGGGAACAtac                                                  >  2:3653327/1‑138 (MQ=2)
                                                                                   tCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCCAACGCCGCCGCCCCCAGCACCCCCGGGCTCTGGCGA‑ACAAAATAGCCGCGGCGCGGTGCCCACGGCTTATACCATAACGCTACCCGGTCGGGCGCGAGGTAACAAcc                                                  >  2:808809/1‑140 (MQ=11)
                                                                                   tCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAACGCCGCCCCCCCCAGCACCCCCGGGCTAAGTCGA‑AAAAAAAACCCGCTGCGGGGTGCCCCCCGGATATACCTAACGGCTACCGGGTCGGGCGCGAGGGAACaaac                                                  >  2:6797949/1‑137 (MQ=2)
                                                                                   tCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAAAGCCCCCCCCCCCAGCACCCCCCCGCTCAGGCGA‑AAAAAAAAGCCCCAGCGCGGTGCCCACGGCGTATACCATAAGGCTACCCGGGCGGGCGCGAGGTaaaaacc                                                  >  1:9991258/1‑135 (MQ=2)
                                                                                        ggCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTa                                             <  2:9926337/140‑1 (MQ=32)
                                                                                                 aGCGC‑GCAGGGGGCGGCCAAACCCCGCCGCCCCCAGCCCCCCCCGGCTAATGCGA‑ACAAAATAGCCCCTGCGCGGTGCCCGCGGCGTATCCCATAAGGCAAACCGGTCGGGCGCGAGGTAACAACCCAGTAAAACGCGcc                                    >  2:929907/1‑140 (MQ=2)
                                                                                                   cgc‑gcAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCt                                  <  1:1672862/140‑1 (MQ=32)
                                                                                                   cgc‑gcAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCt                                  <  1:5518519/140‑1 (MQ=32)
                                                                                                    gc‑gcTGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTc                                 <  1:951106/140‑1 (MQ=17)
                                                                                                    gc‑gcGGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTc                                 <  1:1220795/140‑1 (MQ=17)
                                                                                                        cAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGc                              <  1:9529545/140‑1 (MQ=32)
                                                                                                        cAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGc                              <  1:8851866/140‑1 (MQ=32)
                                                                                                        cAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGc                              <  2:1916741/140‑1 (MQ=32)
                                                                                                             ggcggcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCcaca                         <  2:5931697/140‑1 (MQ=32)
                                                                                                             ggcggcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCcaca                         <  1:6182145/140‑1 (MQ=32)
                                                                                                             ggcggcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCcaca                         <  2:1345544/140‑1 (MQ=32)
                                                                                                             ggcggcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCcaca                         <  2:2875404/140‑1 (MQ=32)
                                                                                                             ggcggcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCcaca                         <  2:7750260/140‑1 (MQ=32)
                                                                                                             ggcggcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCcaca                         <  1:6909529/140‑1 (MQ=32)
                                                                                                                 gcCAATCGCCGCCGCCCCCAGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCGCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCAGTAAAACGCGCCCTCAGCCCACATCCa                     >  1:7695988/1‑140 (MQ=12)
                                                                                                                  ccAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACCTCCAt                    >  1:10446240/1‑140 (MQ=17)
                                                                                                                  ccAAACGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAt                    >  1:7828420/1‑140 (MQ=17)
                                                                                                                     aTCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAtgtg                 >  2:1252124/1‑140 (MQ=32)
                                                                                                                     aTCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAtgtg                 >  1:3910344/1‑140 (MQ=32)
                                                                                                                       cgccgccgcCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGGAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTggg               <  2:607627/140‑1 (MQ=17)
                                                                                                                            ccgccCCCTGCACCCCCGGGCTCTTGCGA‑ACCAATTCGCACCTGCGCGGAGCCCGCGGCTGCTCCCGTGCGTCTACCGCGACGGTCGCGCGGACGCATCCCTGTAAAACGCGCCCTCAGCCCCCCGCCAGGTGGGcagac          >  2:8784479/1‑136 (MQ=2)
                                                                                                                            ccgccCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGcc          >  1:2071720/1‑140 (MQ=32)
                                                                                                                              gccCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGcccc        <  2:4583319/140‑1 (MQ=32)
                                                                                                                              gccCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGcccc        <  1:6223627/140‑1 (MQ=32)
                                                                                                                               cccccTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACTTCCATGTGGGCTGCCCCg       >  2:1758605/1‑140 (MQ=17)
                                                                                                                               cccccTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCg       <  1:8172509/140‑1 (MQ=32)
                                                                                                                               cccccTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCACAGTAAAACGCGCCCTCAGCCCACATCCAGGTGGGCTGCCCCg       >  2:4036101/1‑140 (MQ=12)
                                                                                                                                 cccTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTTGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGc     >  1:8319506/1‑140 (MQ=17)
                                                                                                                                 cccTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGc     <  1:1306273/140‑1 (MQ=32)
                                                                                                                                 cccTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGc     <  2:9550353/140‑1 (MQ=32)
                                                                                                                                    tGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCtt  <  1:10109733/140‑1 (MQ=32)
                                                                                                                                    tGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCtg  >  2:4389806/1‑139 (MQ=17)
                                                                                                                                    |                                                                                                                                            
TAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTT  >  NZ_CP025268/2233108‑2233378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑