Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252682,233,2480GC26.9% 247.2 / 78.9 145intergenic (+148/+225)yohP/dusCsmall membrane protein YohP/tRNA dihydrouridine(16) synthase DusC
Reads supporting (aligned to +/- strand):  ref base G (54/52);  new base C (39/0);  total (93/52)
Fisher's exact test for biased strand distribution p-value = 7.74e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 146 total aligned reads displayed.

GTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAAC  >  NZ_CP025268/2233112‑2233386
                                                                                                                                         |                                                                                                                                           
   atcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCCAAAGCCCCCCCCCCCAGCACCCCCCGGctct                                                                                                                                       >  1:9913442/1‑140 (MQ=37)
    tccggTCCGAAAATAACGAGAATCTTTCCGGCTCTCATCCTGTTGCGCTCCTGTCATGTGCAGTGCTTCCTAGTATCACTGGCGCGAGGAGCGC‑GCAGGGGGCGGGCCCTCGCCGCCGCCCCCGGCACCCCCGGGCGCTg                                                                                                                                      >  2:3678740/4‑140 (MQ=21)
    tcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GGGGGGGGGGGGCAAAAGCCCCCCCCCCCCCGACCCCCCGGcgcag                                                                                                                                      >  1:597375/1‑136 (MQ=255)
        gTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAACGCCGCCGCCCCCAGCACCCCCCGGCGCTGGCGa                                                                                                                                  >  1:4583319/1‑140 (MQ=255)
           cGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACa                                                                                                                              >  2:9529544/1‑140 (MQ=255)
           cGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAACGCCGCCGCCCCCAGCACCCCCCGGCGCTGGCGA‑ACa                                                                                                                              >  2:3706139/1‑140 (MQ=255)
             aaaaTAACGAGAATATTTCAGTCTCTCCTCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCAGCACCCCCGGGCTCTGGCGc‑acaca                                                                                                                            >  2:8851865/1‑138 (MQ=255)
             aaaaTAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACaaa                                                                                                                            >  2:8172508/1‑140 (MQ=255)
                  aaCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GGCGGGGGCGGGCAAAAGCCGCCCCCCCCCGCACCCCCCGGCGCTGGCGC‑ACAAAAAcgc                                                                                                                       >  1:7082540/1‑140 (MQ=25)
                  aaCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATcgc                                                                                                                       >  2:7424523/1‑140 (MQ=255)
                  aaCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATagc                                                                                                                       >  2:10607357/1‑140 (MQ=255)
                   aCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGCGGGGGGGGGCCAA‑CCCCCCCCCCCCCAACCCCCCCCCCCCCTGGCGG‑ACaaaaaaacc                                                                                                                      >  1:5270288/1‑135 (MQ=255)
                          tatTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGCGGCCCAAAGCCCCCCCCCCCACCACCCCCCGGCTCTGGCGA‑ACCAAATAGCCGCAgcgc                                                                                                               >  1:8916100/1‑140 (MQ=21)
                                    tctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCACGGGGCGGCCAAAAGCCCCCGCCCCCAGCACCCCCCGGCACAGGCGA‑AAAAAAAAGCCCCAGCGCCGTGCCCACg                                                                                                     >  2:3503441/1‑140 (MQ=18)
                                      tcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGGGGCCCAACGCCCCCCCCCCCAGCACCCCCCCGCGCTTGCGACACAAAA‑AACCCCAGCGCGGGGCCCTCCGc                                                                                                   >  1:9550354/1‑140 (MQ=16)
                                       cATCCTGTTGCGCTCCTGTCATGTGCATTGCTGCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCCATCGGCGCCGCCCCCAGCACCCACGGGCTCTGGCGT‑ACCAAATCGGCGCTGCGGGGGGGCCTggggg                                                                                                  >  2:9123069/1‑138 (MQ=17)
                                       cATCCTGTGGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGCGGCCAATCGCAGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑AACAACTCGCCGCTGCGCGGTGCCCTCGGCt                                                                                                  >  2:6569557/1‑140 (MQ=18)
                                         cccTGTTGCGCTCCTGTCAATTTCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGAACCCCCGGGCTATGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTa                                                                                                <  2:10500733/139‑1 (MQ=18)
                                              ttGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGGGGCCAAAACCCCCCCCCCCCAGCAACCCCCGGCACAGGCGA‑ACAAAAACCCCCCGGCGCCGTGGCCACCGCGTATacca                                                                                           >  1:4723040/1‑139 (MQ=12)
                                              ttGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATAGCCGCCGCCCCCAGCACCCCCCCGCTCTGGCGA‑ACAAAATAGCCGCAGCGCGGTGCCCACGGCGTATccca                                                                                           >  2:6785481/1‑139 (MQ=18)
                                              ttGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAAACCCCCCCCCCCCAGCACCCCCCGGCACAGGCGA‑ACAAAAAAGCCCCAGCGCGGTGCCCACGGCGTATacca                                                                                           >  2:1156827/1‑139 (MQ=18)
                                                gcgcTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCACCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAACCCtga                                                                                         >  2:9382461/1‑138 (MQ=255)
                                                  gcTCCTGTCTTGTGCATTGGTTCAGATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCCGCGAATCCCCCCGGCCCCCTGGCCCCCCGGGCTCTGGGGC‑ACCAAAACGCCCCAGAGCGGTGGCCTCGGCTTAAACCATACg                                                                                       >  2:9693655/1‑140 (MQ=12)
                                                  gcTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGCCGCCACACCCCCCCGCCCCCAGCACCCCCCGGCCCAGGCGA‑ACAAAAAACCCGCGGCGCGGGGCCCAAGGCATATACCATaag                                                                                       >  2:5285461/1‑138 (MQ=12)
                                                   cTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCATGGGGCGGCCCAACGCCCCCGCCCCCAGCACCCCCCGGCACAGTCGA‑ACAAAATACCCCCGGCGCGGTGCCCACCGCGTATcccgaaccg                                                                                      >  2:291913/1‑134 (MQ=16)
                                                   cTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGTGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCAGGCGA‑ACAAAATCGCCGCTGCTCGGTGCCCACTGCTTATAACTTACgg                                                                                      >  2:4788190/1‑140 (MQ=18)
                                                   cTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAACACCCCCCCCCCCAGCACCCCCCGGCACTTGCCA‑ACAAAAACGCCGCGGCCCGGGGGCCACGGCGTATACCATaacg                                                                                      >  2:4033319/1‑137 (MQ=12)
                                                   cTCATGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCCCCCCCAGCACCCCCGGGCACAGGCGA‑ACAAAAACGCCGCAGCGCGCTGCCCTCGGCATATAACATACgg                                                                                      >  2:4787928/1‑140 (MQ=17)
                                                     ccTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCCATCGCCGCCGCCCCCACCACCCCCCGGCTCTGGCGA‑CCAAAAACGCCGCAGCCCCGTGCCCGCGGCATATCCCATACGGCt                                                                                    >  2:10314666/1‑140 (MQ=17)
                                                      cTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCCATCGCCGCCGCCCCCAGCACCCCCCGGCTCAGGCGA‑ACAAAAACGCCGCGGCGCGGTGCCCTCGGCATATCCCATACGGCTa                                                                                   >  2:7124645/1‑140 (MQ=17)
                                                       tGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTAc                                                                                  <  1:1891231/140‑1 (MQ=255)
                                                            tgtgCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAGCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGt                                                                             <  1:10314667/140‑1 (MQ=255)
                                                              tgCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGCGGCCAAACGCCGCCCCCCCCAGCACCCCCCGGCTCTGGCGA‑ACAAAAACGCCGCTGCGCGGTGCCCACGGCATATCCCTTACGGCTACCGCGTCg                                                                           >  2:9556051/1‑140 (MQ=14)
                                                              tgCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGGGGCCCATCCCCCCCCCCCCCGGCACCCCCCGGCTCTTGCGA‑ACAAAAAACCCCCAGCGCGGTGCCCACCGCGGAACCCATACCGCTAACCGGTCg                                                                           >  2:610776/1‑140 (MQ=9)
                                                              tgCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCg                                                                           >  2:9065514/1‑140 (MQ=255)
                                                              tgCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAACGCCGCCCCCCCCAGCACCCCCCGGCGCAGGCGA‑ACCAAATCCCCGCAGCGCCGTGCCCCCGGCATATCCCATACCGCAACCCGGTCg                                                                           >  2:1891231/1‑140 (MQ=14)
                                                                cATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCggg                                                                         <  1:7918104/140‑1 (MQ=255)
                                                                cATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCggg                                                                         <  2:3202409/140‑1 (MQ=255)
                                                                cATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCggg                                                                         <  1:1530031/140‑1 (MQ=255)
                                                                 aTTGCTTCATATAATCCCTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGgc                                                                        <  2:2241384/140‑1 (MQ=255)
                                                                  ttGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGgcg                                                                       <  1:3191903/140‑1 (MQ=255)
                                                                   tGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGCGGCCCAACCCCCCCCCCCCCCCCACCCCCCGGCACTGGCCA‑CCAAAAACCCCCCAGCGCGGGGCCCGCCGCGTATCCCATAAGGCTACCCGG‑CGGTcgcg                                                                     >  2:1338264/1‑140 (MQ=9)
                                                                   tGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCCCCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCGCGGCTTATCCCTTACGGCGACCGGGTCGGgcgc                                                                      >  2:7169724/1‑140 (MQ=255)
                                                                   tGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAACGCCGCCGCCCCCTGCCCCCCCCGGCTCTGGCGA‑ACAAAATAGCCGCAGCGCGGGGCCCACGGCGTATCCCAAACGGCTACCCGGTCGGgcgc                                                                      >  2:6223627/1‑140 (MQ=14)
                                                                   tGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAACGCCGCCGCCCCCAGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCCCTGCGCGGTGCCCACGGCTTATCCCATACGGCTACCCGGTCGGgcgc                                                                      >  2:5667727/1‑140 (MQ=14)
                                                                   tGCTTCAGATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGGCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑CCAAAATCGCCGCTGCGCGGTGCCCACGGCTTATCCCTTACGGCTCCCCGGTCGGgcgc                                                                      >  2:9799124/1‑140 (MQ=14)
                                                                      ttCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCAGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCACGGCGTATCCCATACGGCAACCCGGTCGGGCGCGAg                                                                   >  2:6543419/1‑140 (MQ=14)
                                                                          tataATCCCTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTaa                                                               <  1:6785482/140‑1 (MQ=37)
                                                                          tataATCACTGGCGCAAGGAGCGC‑GGCGGGGGCGGCCAAAAGCCGCCCCCCCCAGCACCCCCCGGCTCTGGGGC‑ACAAAATAGCCGCAGCGCGGTGCCCACGGCGTATACCATAAGGCGACCCGGGGGGGCGCGAGGGaa                                                               >  2:4394736/1‑140 (MQ=2)
                                                                          tataATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCAGCCCCCTGCACACCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTAACGGGTCGGGCGCGAGGTaa                                                               <  1:624283/140‑1 (MQ=255)
                                                                          tataATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAAAGCCGCCGCCCCCAGCACCCCCCGGCTCTGGCGA‑ACCAAATCGCCCCGGCGCGGTGCCCGCGGCTGATACCGTAAGGCTACCCGGTCGGGCGCGAGGTaa                                                               >  2:8693784/1‑140 (MQ=11)
                                                                            taATCCCTGGCGCAAGGAGCGC‑GCCGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACa                                                             <  1:1156827/140‑1 (MQ=21)
                                                                             aaTCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACAt                                                            <  1:8693785/140‑1 (MQ=34)
                                                                             aaTCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACAt                                                            <  2:3778388/140‑1 (MQ=34)
                                                                               tCACTGGCGCAAGGAGCGC‑GGCGGGGGCGGCCAAACGCCGCCCCCCCCAGCACCCCCCGGCTCTGGCGC‑ACAAAAAAGCCCCCGCGCCGGGCCCCCCGCCTATACCCAACCGCTAACCGGGCGGGCGCCAGGGAACAAcc                                                          >  2:8469084/1‑140 (MQ=255)
                                                                               tCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCCAACGCCGCCGCCCCCAGCACCCCCGGGCTCTGGCGA‑ACAAAATAGCCGCGGCGCGGTGCCCACGGCTTATACCATAACGCTACCCGGTCGGGCGCGAGGTAACAAcc                                                          >  2:808809/1‑140 (MQ=11)
                                                                               tCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGGCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGGAACagac                                                          >  2:9080420/1‑137 (MQ=16)
                                                                               tCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCAGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTAAGGCTACCGGGTCGGGCGCGAGGGAACATcc                                                          >  2:6449900/1‑140 (MQ=16)
                                                                               tCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAACGCCGCCCCCCCCAGCACCCCCGGGCTAAGTCGA‑AAAAAAAACCCGCTGCGGGGTGCCCCCCGGATATACCTAACGGCTACCGGGTCGGGCGCGAGGGAACaaac                                                          >  2:6797949/1‑137 (MQ=2)
                                                                               tCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAAAGCCCCCCCCCCCAGCACCCCCCCGCTCAGGCGA‑AAAAAAAAGCCCCAGCGCGGTGCCCACGGCGTATACCATAAGGCTACCCGGGCGGGCGCGAGGTaaaaacc                                                          >  1:9991258/1‑135 (MQ=2)
                                                                                  cTGGCGCAAGGAGCGC‑GGAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCCCCCCTGCGCGGTGGCCGCGGCGTATCCCTTACGGCTACCCGGTCGGGCGCGTGGTAACCTcccag                                                       >  2:4528095/1‑138 (MQ=11)
                                                                                    ggCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTa                                                     <  2:9926337/140‑1 (MQ=32)
                                                                                               cgc‑gcAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCt                                          <  1:1672862/140‑1 (MQ=32)
                                                                                               cgc‑gcAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCt                                          <  1:5518519/140‑1 (MQ=32)
                                                                                               cgc‑gcAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCt                                          <  1:5620300/140‑1 (MQ=32)
                                                                                                gc‑gcGGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTc                                         <  1:1220795/140‑1 (MQ=17)
                                                                                                    cAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGc                                      <  2:3960937/140‑1 (MQ=32)
                                                                                                    cAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGc                                      <  1:9529545/140‑1 (MQ=32)
                                                                                                     aGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGcc                                     <  2:4166121/140‑1 (MQ=32)
                                                                                                         ggcggcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCcaca                                 <  2:2875404/140‑1 (MQ=32)
                                                                                                         ggcggcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCcaca                                 <  2:1345544/140‑1 (MQ=32)
                                                                                                         ggcggcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCcaca                                 <  2:5931697/140‑1 (MQ=32)
                                                                                                         ggcggcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCcaca                                 <  1:6182145/140‑1 (MQ=32)
                                                                                                         ggcggcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCcaca                                 <  2:7750260/140‑1 (MQ=32)
                                                                                                         ggcggcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCcaca                                 <  1:6909529/140‑1 (MQ=32)
                                                                                                             gcCAATCGCCGCCGCCCCCAGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCGCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCAGTAAAACGCGCCCTCAGCCCACATCCa                             >  1:7695988/1‑140 (MQ=12)
                                                                                                              ccAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACCTCCAt                            >  1:10446240/1‑140 (MQ=17)
                                                                                                                 aTCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAATTCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAGCATCCCTGTAAATCGCGCCCTCAGCCCACATCCAtgtg                         >  2:6121666/1‑140 (MQ=12)
                                                                                                                 aTCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAtgtg                         >  1:3910344/1‑140 (MQ=32)
                                                                                                                 aTCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAtgtg                         >  2:1252124/1‑140 (MQ=32)
                                                                                                                   cgccgccgcCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTggg                       >  2:240547/1‑140 (MQ=32)
                                                                                                                        ccgccCCCTGCACCCCCGGGCTCTTGCGA‑ACCAATTCGCACCTGCGCGGAGCCCGCGGCTGCTCCCGTGCGTCTACCGCGACGGTCGCGCGGACGCATCCCTGTAAAACGCGCCCTCAGCCCCCCGCCAGGTGGGcagac                  >  2:8784479/1‑136 (MQ=2)
                                                                                                                        ccgccCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGcc                  >  1:5264103/1‑140 (MQ=32)
                                                                                                                          gccCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGcccc                <  1:6223627/140‑1 (MQ=32)
                                                                                                                           cccccTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACTTCCATGTGGGCTGCCCCg               >  2:1758605/1‑140 (MQ=17)
                                                                                                                           cccccTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCg               <  1:9065515/140‑1 (MQ=32)
                                                                                                                           cccccTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCg               <  1:8172509/140‑1 (MQ=32)
                                                                                                                           cccccTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCACAGTAAAACGCGCCCTCAGCCCACATCCAGGTGGGCTGCCCCg               >  2:4036101/1‑140 (MQ=12)
                                                                                                                             cccTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTTGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGc             >  1:8319506/1‑140 (MQ=17)
                                                                                                                                tGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCtt          <  1:10109733/140‑1 (MQ=32)
                                                                                                                                  cACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCt                                          <  1:7384081/106‑1 (MQ=32)
                                                                                                                                  cACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCa        >  1:4315088/1‑140 (MQ=32)
                                                                                                                                     ccccGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAggg     >  1:7939046/1‑140 (MQ=32)
                                                                                                                                       ccGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGac   >  1:4191047/1‑139 (MQ=17)
                                                                                                                                       ccGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGaa   >  2:6245875/1‑140 (MQ=32)
                                                                                                                                       ccGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGaa   >  1:8779317/1‑140 (MQ=32)
                                                                                                                                        cGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAcg                                                <  1:1923966/94‑1 (MQ=33)
                                                                                                                                        cGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAcg                                                >  2:1923966/1‑94 (MQ=33)
                                                                                                                                        cGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAAc  >  2:537306/1‑140 (MQ=32)
                                                                                                                                        cGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAAc  >  1:418486/1‑140 (MQ=32)
                                                                                                                                         |                                                                                                                                           
GTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGA‑ACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAAC  >  NZ_CP025268/2233112‑2233386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑