Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252683,505,5530TA26.0% 472.1 / 39.5 237intergenic (+84/+68)phoB/yhfSDNA‑binding transcriptional regulator FrlR/aminotransferase class I/II‑fold pyridoxal phosphate‑dependent enzyme
Reads supporting (aligned to +/- strand):  ref base T (42/123);  new base A (57/1);  total (110/127)
Fisher's exact test for biased strand distribution p-value = 1.04e-24
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 238 total aligned reads displayed.

ACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTA‑GCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATG  >  NZ_CP025268/3505415‑3505690
                                                                                                                                          |                                                                                                                                          
aCTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTa                                                                                                                                           <  2:7559170/140‑1 (MQ=255)
  tcttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCTCACCCAAAc                                                                                                                                         >  1:4634473/1‑140 (MQ=255)
   cttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAAcc                                                                                                                                        <  1:4067717/140‑1 (MQ=255)
    ttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCTAAccc                                                                                                                                       >  1:7376755/1‑140 (MQ=255)
    ttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATACGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCTCACCCTAAccc                                                                                                                                       >  2:1495120/1‑140 (MQ=255)
     tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCct                                                                                                                                      <  2:8579029/140‑1 (MQ=255)
     tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCct                                                                                                                                      <  2:1075014/140‑1 (MQ=255)
     tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCct                                                                                                                                      <  1:9610992/140‑1 (MQ=255)
       tGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCAAAcccaca                                                                                                                                    >  1:6360780/1‑137 (MQ=255)
            cgcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCa                                                                                                                               <  1:4615956/140‑1 (MQ=255)
              ccAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACACCCAAAACCACACCCCaaa                                                                                                                             >  1:496357/1‑140 (MQ=38)
              ccAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCGAACCCGCAcccccac                                                                                                                             >  1:8504492/1‑137 (MQ=39)
                aaTCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCaccgg                                                                                                                           <  1:4722843/140‑5 (MQ=255)
                aaTCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAgg                                                                                                                           <  1:10738595/140‑1 (MQ=255)
                aaTCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAgg                                                                                                                           <  2:8773647/140‑1 (MQ=255)
                     cATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGa                                                                                                                      <  1:7571720/140‑1 (MQ=255)
                     cATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGa                                                                                                                      <  1:10206476/140‑1 (MQ=255)
                          ccTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGa                                                                                                                 <  2:142123/140‑1 (MQ=255)
                          ccTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGa                                                                                                                 <  1:6008624/140‑1 (MQ=255)
                          ccTTAACTAGTGAAAATAAAAGACATTAACCGTCGGCCGGATAAGATGCGCCAGCATCGCCTCCGGCGATGCTGGCGCGATGAATTTTACATCCCGTACGTTCCCCTCACCCTAACACTCTCCCCAAAGGGGCGAGGGGa                                                                                                                 <  2:6268289/140‑1 (MQ=38)
                            ttAACGATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGAcc                                                                                                               <  1:6970527/140‑1 (MQ=255)
                             tAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAAACCACAACCCAAAAGGGGGCGGGGGccc                                                                                                              >  1:5113141/1‑139 (MQ=37)
                               ccgcttGATAATAAAAGACAGTAACCGGAGGCCGGATAAGATGCGCCCGCATCGCCTCCGGCGAGGCTGGCGCGTTGAATTTTACAACCCGTACGGTCCCCTCACCCTAACCCACTCCCCAAAGGGGCGAGGGGACCGtt                                                                                                            <  2:7919960/136‑1 (MQ=37)
                               aCTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGtt                                                                                                            <  1:3221671/140‑1 (MQ=255)
                               aCTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGtt                                                                                                            <  1:1897753/140‑1 (MQ=255)
                                cTATTGATAATAAAAGACATTAACCGTAGGCCGGATAATATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCAAACCCTCTCCCCAATGGGGCGAGGGGACCGGTc                                                                                                           >  2:5889066/1‑140 (MQ=39)
                                   ttGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTGGACTGTTTCATCCCGTACGTGACCCGGACCCTCACGCGCTACCGAAAGGGGCGAGGGGTCCGTTGAgg                                                                                                        >  2:7797307/1‑139 (MQ=25)
                                    tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACCCCCAAAACCAAGACCCAAAGGGGCGAGGGGGCCGTTAAGTa                                                                                                       >  2:1949486/1‑140 (MQ=37)
                                    tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTa                                                                                                       <  1:2798164/140‑1 (MQ=255)
                                    tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTACCCAAAGGGGCGCGGGGACCGTTCAGTa                                                                                                       >  1:7779426/1‑140 (MQ=255)
                                    tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCAAACCCTCGCCCCCAAGGGGCGCGGGGACCGGTCAggg                                                                                                       >  1:10394647/1‑138 (MQ=38)
                                    tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACACCCAAACCCACACCCCAAAGGGGCGAGGGGACCGGTCAGTa                                                                                                       >  1:8747927/1‑140 (MQ=38)
                                    tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTGGCCCACAACCAAAACCTAACACCAAACGGGCGTGGGGGCCGTTGAGTa                                                                                                       >  1:8386776/1‑140 (MQ=25)
                                    tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATACGGCGATGCTGGATCGTTGAATTTTACATCCCGTACGTTCCCCAAACCCCAACACACACCCCAAACGGGGGTGGGGAGCGTTCACTa                                                                                                       >  1:7721722/1‑140 (MQ=25)
                                     gATAATAAAAGACATTAACCGTAGGCCTGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACACCCAAAACCACACCCCAAACGGGCGAGGGGACCGTTCAGTaa                                                                                                      >  2:5628215/1‑139 (MQ=38)
                                     gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCTCACCCTAACCCACTCCCCCAAGGGGCGTGGGGTCCGTTCAGTAc                                                                                                      >  1:217547/1‑140 (MQ=39)
                                     gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTAc                                                                                                      >  1:7054372/1‑140 (MQ=255)
                                     gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAACCCACACCCCAAAGGGGCGAGGGGACCGTTCAGTAc                                                                                                      >  2:8407662/1‑140 (MQ=255)
                                     gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAAACCTCTCCCCAAAGGGGCGAGGGGACCGTTAAGGAc                                                                                                      >  2:8152661/1‑140 (MQ=255)
                                     gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCAACCTAACCCTCTCCCCCAAGGGGCGTGGGGACCGTTCAGTAc                                                                                                      >  2:10332481/1‑140 (MQ=255)
                                             aaGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACCCCCAAAACCTCACCCCAAATGGGCGAGGGGACCCTTCAGTACGGAAATTa                                                                                              >  1:10692834/1‑140 (MQ=38)
                                              aGACATGAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCTCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTA‑g                                                                                            <  1:8112771/140‑1 (MQ=255)
                                               gACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTA‑Gc                                                                                           <  2:3163274/140‑1 (MQ=255)
                                                  aTTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTA‑GCTTg                                                                                        >  2:7278195/1‑140 (MQ=255)
                                                  aTTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTA‑GCTTg                                                                                        >  2:9749067/1‑140 (MQ=255)
                                                  aTTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTA‑GGTTg                                                                                        >  1:9809970/1‑140 (MQ=255)
                                                   ttAACCGTCGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAACCCGCACCCCAAAGGGGCGAGGGGACCGGTCAGTACGGACTTTA‑GCtata                                                                                       >  2:4244483/1‑137 (MQ=38)
                                                   ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTA‑GCTTGa                                                                                       <  2:2394420/140‑1 (MQ=255)
                                                   ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTA‑GCTTGa                                                                                       <  2:2238557/140‑1 (MQ=255)
                                                   ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTA‑GCTTGa                                                                                       <  1:2935429/140‑1 (MQ=255)
                                                   tgaaCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGATGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTA‑GCTTGa                                                                                       <  2:6905763/138‑1 (MQ=255)
                                                   acaaCCGTAGGCCGGATAAGATGCGCCAGCAACGCTTCCGGCGATGCCGGCGCGTTGAATATTACATCCCGTACGTTCCCCTCACCCTCACCCTCTCCCCACAGGGGCGAGTGGACCGTTCAGTACGGAATTTA‑GCTTGa                                                                                       <  1:9912999/138‑1 (MQ=38)
                                                        cGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCCCCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTA‑GCTTGACGGTg                                                                                  <  1:9433872/140‑1 (MQ=255)
                                                        cGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTA‑GCTTGACGGTg                                                                                  <  2:2969665/140‑1 (MQ=255)
                                                        cGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTA‑GCTTGACGGTg                                                                                  <  2:5505494/140‑1 (MQ=255)
                                                        cGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCCCAGGGGCGAGGGGACCGTTCAGTACGGATTTTA‑GCTTGACGGTg                                                                                  <  2:2748107/140‑1 (MQ=255)
                                                         gTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGAACGTTCAGTACGGAATTTA‑GCTTGACGGTGc                                                                                 <  2:1366191/140‑1 (MQ=255)
                                                         gTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAACCCTCTCCCCAAAGGGGCGAGGGGAACGTTCAGTACGGAATTTA‑GCTTGACGGTGc                                                                                 >  2:4739350/1‑140 (MQ=255)
                                                          tAGGCCGGATAAGATGCGCCAGCAGCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCGCACCCAAAACCTCACCACAAAGGGGCGCTGGGACCGTTCACTACGGAAATTG‑TCTTGACGGTGCt                                                                                >  2:3040678/1‑140 (MQ=37)
                                                           aGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAAGTTA‑TCTTTACGGTGCTa                                                                               >  2:4726078/1‑140 (MQ=38)
                                                            ggCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTGGCCCCCACCCAAAACCACAACCCAAAAGGGCGGGGGGGCCCGGGAGTACGGGATTTATGTTGGACGGTggat                                                                               >  1:4180078/1‑137 (MQ=16)
                                                             gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTA‑GCTTGACGGTGCTATc                                                                             <  2:7054371/140‑1 (MQ=255)
                                                             gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTA‑GCTTGACGGTGCTATc                                                                             <  2:1129065/140‑1 (MQ=255)
                                                             gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTA‑GCTTGACGGTGCTATc                                                                             <  2:9003010/140‑1 (MQ=255)
                                                             gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTA‑GCTTGACGGTGCTATc                                                                             <  1:5303628/140‑1 (MQ=255)
                                                             gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCACCAACGGGGCGAGGGGACCGTTCAGTACGGAAGTGA‑GCTTGACGGTGCTATc                                                                             <  2:5327082/140‑1 (MQ=255)
                                                               cGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTA‑GCTTGACGGTGCTATCCa                                                                           <  1:8152662/140‑1 (MQ=255)
                                                               cGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTA‑GCTTGACGGTGCTATCCa                                                                           <  1:3245816/140‑1 (MQ=255)
                                                               cGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTA‑GCTTGACGGTGCTATCCa                                                                           <  1:3462707/140‑1 (MQ=255)
                                                               cGGAGAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGACTTTTACATCCCGTACGTTCCCCTCACCCTCACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTA‑GCTTGACGGTGCTATCCa                                                                           <  1:6639677/140‑1 (MQ=255)
                                                                  aTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCTCACCCCAACCCACTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTA‑TCTTTACGGTGCTATCCAGCa                                                                        >  1:10818891/1‑140 (MQ=39)
                                                                  aTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTA‑GCTTGACGGTGCTATCCAGCa                                                                        <  2:8946594/140‑1 (MQ=255)
                                                                  aTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTGCCCCCACCCAAACCCACTACCCCAACGGGGGAGGGGGCCCGTCAGTACGGAATTTA‑GCTTGACGGTGCTATACAGCa                                                                        >  1:6395456/1‑140 (MQ=255)
                                                                       aTGCGCCAGCATCGCATCCTGCGATGCTGGCGCGTTTAATCTTACATCCCGTACGTTAACCAAACCCTAAACCACTACCCAAAGTGGCGAGGGGAACCTTAAGTACAGAAATTA‑TCTAGACGGTGCTAACAATCATTaaa                                                                   >  2:9430371/1‑140 (MQ=255)
                                                                        tGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTACCCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTA‑GCTTGACGGTGCTATCCAGCATTAAAt                                                                  <  1:6484993/140‑1 (MQ=255)
                                                                           gcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCACAGGGGCGAGGGGACCGTTCAGTACGGAATTTA‑GCTTGACGGTGCTATCCAGCATTAAATACt                                                               <  1:3794222/140‑1 (MQ=255)
                                                                           gcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCTAACCCTCACCCCAAAGGGGCGTGGGGACCGTTCAGTACGGAATTTT‑GGGTGACGGTGGTATCCAGCATTTAATACt                                                               >  1:9164691/1‑140 (MQ=38)
                                                                           gcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACACCCAAACCCAAAACCCCAAGGGGCGAGGGGACCGTTCAGTACGGAATTTA‑GCTTTTAGGTGCTAAACAGCATTataaaat                                                               >  2:2112225/1‑134 (MQ=255)
                                                                            ccAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCTCACCCTAAACCTCAACCCAAAGGGGCGAGGGGTCCGGTCAGTTCGGAAATTA‑GCTTTAAGGTGGGATACAGCATTAAATACTg                                                              >  2:3932657/1‑140 (MQ=255)
                                                                             cAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCCCCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTA‑GCTTGACGGTGCTATCCAGCATTAAATACTgg                                                             <  2:1689286/140‑1 (MQ=255)
                                                                                catcgcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAAACCACTACCCCAAGGGGCGAGGGGACCGGTCAGTACGGAATTTT‑TATTGAAGGTGCTATACACCAATAAAAAAAGGCaa                                                          >  2:3591467/1‑140 (MQ=255)
                                                                                     catcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAACCCTCAACCCCAAGGGGCGAGGGGCCCGTTCCGTTCGGAATTTT‑GCTTGACGGTGCTATACAGCATTAAATACAGGGAATTcta                                                     >  2:7520834/1‑139 (MQ=255)
                                                                                      atcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTA‑GCTTGACGGTGCTATCAAGCATTAAATACTGGCAATActct                                                    <  2:2688249/140‑1 (MQ=255)
                                                                                          ggCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTGGCCCACACCCAAACCCACACCCCAAAGGGGCGGGGGGGGCGGTGAGTACGGAATTTT‑GCTTGACGGTGCGATACAGCATTAAAAAATTGGAATActaaaaac                                                >  1:8439909/1‑134 (MQ=255)
                                                                                            cGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTA‑GCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCa                                              <  1:5679214/140‑1 (MQ=255)
                                                                                              aTGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTA‑GCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCaaa                                            <  1:5863598/140‑1 (MQ=255)
                                                                                                  tGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTA‑GCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATcc                                        <  1:1699292/140‑1 (MQ=255)
                                                                                                  tGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAAACCACTACCCAAATGGGCGAGGGGACCGTTCAGTACGGAATTTA‑GCTTGACGGTGCTATACAGCATTAAATACAGGCAAAAATCACACGCaaaaaac                                        >  1:10031879/1‑137 (MQ=255)
                                                                                                       cgTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTA‑GCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCgcagc                                   <  1:6819643/140‑1 (MQ=255)
                                                                                                         ttGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTA‑GCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCAc                                 <  2:61895/140‑1 (MQ=255)
                                                                                                         ttGAATTTTACATCCCGTACGTTCCCCTCACCCGAACCCTCTCCCCAAAGGGGCTAGGGGACCGTTCAGTACGGAATTTA‑GCTTGACGGTGCTAGCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCAc                                 <  2:6864362/140‑1 (MQ=255)
                                                                                                          tGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTA‑GCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCAcc                                <  1:5490561/140‑1 (MQ=255)
                                                                                                                 tACATCCCGTACGTTACCCTCACCCTAAACCTCTCCCCAAATGGGCGAGGGGACCGTTCAGTACGGAATTTA‑GCTTGACGGTGCTATCAAGCATTAAATAATGGCAATACTCTCCCGCAAAATACGCAGCTCCGTCTCtt                         >  2:9739207/1‑140 (MQ=255)
                                                                                                                        cGTACGTTACCCACACCCAAAACCACACCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTA‑TCGTTAAGGTGCTATCCAGCATTAAATAATGGCAAAAAACACACGCAAAAACCGCAGCACCCTCTCATCACCGca                  >  2:6746077/1‑139 (MQ=255)
                                                                                                                          tACGTTCCCCTCACCCAAAACCACACCCCCAATGGGCGAGGGGACCGTTCAGTACGGAATTTA‑GCTTGACGGTGCAATACAGCAGTAAAAACTGTCAATACTCTAACGCACAAAACGCAGCACCCTCGATTCACCGCTGc                >  2:651034/1‑140 (MQ=255)
                                                                                                                           aCGTTCCCCTCACCCTAAACCACACCCCAAATGGGCGAGGGGACCGTTCATTACGGAATTTA‑GCATGACGGTGCTATCCAGCATTAAATACTTGCAATACTCAAACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCg               >  2:6315242/1‑140 (MQ=255)
                                                                                                                             gTTCCCCTCACCCTAACCCTCTCCCCACAGGGGCGAGGGGACCGGTCAGTACGGAATTTA‑GCTTGACGGTGCTAACCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGAt             <  2:7359883/140‑1 (MQ=255)
                                                                                                                                  ccTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTA‑GCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCGGCGCTTCggg        >  2:8286065/1‑140 (MQ=255)
                                                                                                                                   cTCACCCTAACCCTAACCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAAATTA‑TCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACACACACGCAAAATCCGCACCACCCTCACATCACCGCAGCGAATCGGGt       >  2:10490390/1‑140 (MQ=37)
                                                                                                                                   cTCACCCAAAACCACACCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAAATTA‑GCTTGACGGTTCTATCCAGCAATAAATACTGGCAAAACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGt       >  2:8784134/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                          
ACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTA‑GCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATG  >  NZ_CP025268/3505415‑3505690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑