BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,505,559	0	T	A	22.5%	476.9 / 46.3	231	intergenic (+90/+62)	phoB/yhfS	DNA‑binding transcriptional regulator FrlR/aminotransferase class I/II‑fold pyridoxal phosphate‑dependent enzyme
Reads supporting (aligned to +/- strand): ref base T (53/119); new base A (48/2); total (110/121)
Fisher's exact test for biased strand distribution p-value = 7.61e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 248 total aligned reads displayed.

TCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGC  >  NZ_CP025268/3505420‑3505698

tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCct                                                                                                                                             <  1:9610992/140‑1 (MQ=255)

tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCct                                                                                                                                             <  2:1075014/140‑1 (MQ=255)

tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCct                                                                                                                                             <  2:8579029/140‑1 (MQ=255)

    ccGCGCCCATCGCATCACCTTCACTATGGATAATAAAAGACATTAACCGTAGGCCGGATAAGAGGCGCCAGCATCGCATCCGGCGATGCTTGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTcc                                                                                                                                         <  2:8943831/140‑1 (MQ=39)

         ccAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACACCCAAAACCACACCCCaaa                                                                                                                                    >  1:496357/1‑140 (MQ=38)

           aaTCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCaccgg                                                                                                                                  <  1:4722843/140‑5 (MQ=255)

           aaTCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAgg                                                                                                                                  <  1:10738595/140‑1 (MQ=255)

                cATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGa                                                                                                                             <  1:10206476/140‑1 (MQ=255)

                     ccTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGa                                                                                                                        <  1:6008624/140‑1 (MQ=255)

                     ccTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGa                                                                                                                        <  1:4296330/140‑1 (MQ=255)

                     ccTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGa                                                                                                                        <  2:6278192/140‑1 (MQ=255)

                     ccTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGa                                                                                                                        <  1:8407663/140‑1 (MQ=255)

                     ccTTAACTAGTGAAAATAAAAGACATTAACCGTCGGCCGGATAAGATGCGCCAGCATCGCCTCCGGCGATGCTGGCGCGATGAATTTTACATCCCGTACGTTCCCCTCACCCTAACACTCTCCCCAAAGGGGCGAGGGGa                                                                                                                        <  2:6268289/140‑1 (MQ=38)

                       ttAACGATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGAcc                                                                                                                      <  1:6970527/140‑1 (MQ=255)

                          ccgcttGATAATAAAAGACAGTAACCGGAGGCCGGATAAGATGCGCCCGCATCGCCTCCGGCGAGGCTGGCGCGTTGAATTTTACAACCCGTACGGTCCCCTCACCCTAACCCACTCCCCAAAGGGGCGAGGGGACCGtt                                                                                                                   <  2:7919960/136‑1 (MQ=37)

                          aCTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGtt                                                                                                                   <  1:1897753/140‑1 (MQ=255)

                          aCTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGtt                                                                                                                   <  1:3221671/140‑1 (MQ=255)

                              ttGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTGGACTGTTTCATCCCGTACGTGACCCGGACCCTCACGCGCTACCGAAAGGGGCGAGGGGTCCGTTGAgg                                                                                                               >  2:7797307/1‑139 (MQ=25)

                               tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGTGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTa                                                                                                              >  2:8743243/1‑140 (MQ=255)

                               tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACCCCCAAAACCAAGACCCAAAGGGGCGAGGGGGCCGTTAAGTa                                                                                                              >  2:1949486/1‑140 (MQ=37)

                               tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTa                                                                                                              <  1:2798164/140‑1 (MQ=255)

                               tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCAAACCCTCGCCCCCAAGGGGCGCGGGGACCGGTCAggg                                                                                                              >  1:10394647/1‑138 (MQ=38)

                               tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATACGGCGATGCTGGATCGTTGAATTTTACATCCCGTACGTTCCCCAAACCCCAACACACACCCCAAACGGGGGTGGGGAGCGTTCACTa                                                                                                              >  1:7721722/1‑140 (MQ=25)

                                gATAATAAAAGACATTAACCGTAGGCCTGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACACCCAAAACCACACCCCAAACGGGCGAGGGGACCGTTCAGTaa                                                                                                             >  2:5628215/1‑139 (MQ=38)

                                gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCAACCTAACCCTCTCCCCCAAGGGGCGTGGGGACCGTTCAGTAc                                                                                                             >  2:10332481/1‑140 (MQ=255)

                                   aataaAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAACCCACAACCCAAAGGGGCCAGGGGCCCCTTCAGTACCGa                                                                                                          >  1:8137090/1‑140 (MQ=37)

                                     taaAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAAt                                                                                                        <  1:3473124/140‑1 (MQ=255)

                                        aaGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACCCCCAAAACCTCACCCCAAATGGGCGAGGGGACCCTTCAGTACGGAAATTa                                                                                                     >  1:10692834/1‑140 (MQ=38)

                                         aGACATGAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCTCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAg                                                                                                    <  1:8112771/140‑1 (MQ=255)

                                          gACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGc                                                                                                   <  2:3163274/140‑1 (MQ=255)

                                             aTTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTg                                                                                                >  2:9749067/1‑140 (MQ=255)

                                             aTTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGGTTg                                                                                                >  1:9809970/1‑140 (MQ=255)

                                             aTTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCTCCCACAAACCCAAACCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTATCTTg                                                                                                >  2:730084/1‑140 (MQ=38)

                                              ttAACCGTCGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAACCCGCACCCCAAAGGGGCGAGGGGACCGGTCAGTACGGACTTTAGCtata                                                                                               >  2:4244483/1‑137 (MQ=38)

                                              ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                               <  2:3482950/140‑1 (MQ=255)

                                              ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                               <  2:2394420/140‑1 (MQ=255)

                                              ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                               <  1:2935429/140‑1 (MQ=255)

                                              ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                               <  1:6516478/140‑1 (MQ=255)

                                              ttAACCGGAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                               <  1:215216/140‑1 (MQ=255)

                                              acaaCCGTAGGCCGGATAAGATGCGCCAGCAACGCTTCCGGCGATGCCGGCGCGTTGAATATTACATCCCGTACGTTCCCCTCACCCTCACCCTCTCCCCACAGGGGCGAGTGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                               <  1:9912999/138‑1 (MQ=38)

                                                aaCCGTAGGCCGGATAAGATGCGCCAGCATCTCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCACCTCACCCTAACCCTCTCCCCAAAGGGGCGTGGGGACCGTTCAGTACGGAATTTAGCTTGACg                                                                                             <  2:8154783/140‑1 (MQ=255)

                                                aaCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTGCCCACACCCAAAACCAAAACCCAAAGGGGCGCGGGGACCGTTCAGTACGGAAATTATCtttaag                                                                                             >  1:5473924/1‑136 (MQ=25)

                                                   cGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                          <  1:1629257/140‑1 (MQ=255)

                                                   cGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                          <  2:2969665/140‑1 (MQ=255)

                                                   cGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                          <  2:750673/140‑1 (MQ=255)

                                                   cGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCCCAGGGGCGAGGGGACCGTTCAGTACGGATTTTAGCTTGACGGTg                                                                                          <  2:2748107/140‑1 (MQ=255)

                                                    gTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGAACGTTCAGTACGGAATTTAGCTTGACGGTGc                                                                                         <  2:1366191/140‑1 (MQ=255)

                                                    gTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAACCCTCTCCCCAAAGGGGCGAGGGGAACGTTCAGTACGGAATTTAGCTTGACGGTGc                                                                                         >  2:4739350/1‑140 (MQ=255)

                                                     tAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCAAACCCACTACCCAAAGGGGCGAGGGGACCGTTCAGTACGGGATTTATCTTGTCGGTGCt                                                                                        >  1:6363193/1‑140 (MQ=38)

                                                     tAGGCCGGATAAGATGCGCCAGCAGCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCGCACCCAAAACCTCACCACAAAGGGGCGCTGGGACCGTTCACTACGGAAATTGTCTTGACGGTGCt                                                                                        >  2:3040678/1‑140 (MQ=37)

                                                      aGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAAGTTATCTTTACGGTGCTa                                                                                       >  2:4726078/1‑140 (MQ=38)

                                                       ggCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAACCCACAACCCCAAAGGGCGAGGGGACCCGTAAGAACCGAAATTAGATTTACGGTGGTaa                                                                                      >  1:5597750/1‑139 (MQ=25)

                                                        gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATc                                                                                     <  2:9003010/140‑1 (MQ=255)

                                                        gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATc                                                                                     <  2:7054371/140‑1 (MQ=255)

                                                        gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATc                                                                                     <  2:1129065/140‑1 (MQ=255)

                                                        gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCACCAACGGGGCGAGGGGACCGTTCAGTACGGAAGTGAGCTTGACGGTGCTATc                                                                                     <  2:5327082/140‑1 (MQ=255)

                                                          cGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCACAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCa                                                                                   <  1:6639736/140‑1 (MQ=255)

                                                          cGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCa                                                                                   <  1:467732/140‑1 (MQ=255)

                                                          cGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCa                                                                                   <  1:3245816/140‑1 (MQ=255)

                                                          cGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCa                                                                                   <  1:6946108/140‑1 (MQ=255)

                                                             aTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAAATTAGCATGACGGTGCTATCCAGCa                                                                                >  2:6529164/1‑140 (MQ=255)

                                                              tacgATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGATGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCCAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCAt                                                                               <  2:6845514/137‑1 (MQ=255)

                                                                  aTGCGCCAGCATCGCATCCTGCGATGCTGGCGCGTTTAATCTTACATCCCGTACGTTAACCAAACCCTAAACCACTACCCAAAGTGGCGAGGGGAACCTTAAGTACAGAAATTATCTAGACGGTGCTAACAATCATTaaa                                                                           >  2:9430371/1‑140 (MQ=255)

                                                                   tGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTACCCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAAt                                                                          <  1:4103141/140‑1 (MQ=255)

                                                                   tGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAAt                                                                          <  2:1667132/140‑1 (MQ=255)

                                                                      gcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACACCCAAAACCCCTCCCCAAAGGGGCGAGGGGGCCCGTAAGTAAGGGATTTATATTGAGGGGGGTATACAGCATTaaaaaat                                                                       >  1:5297853/1‑136 (MQ=255)

                                                                      gcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCACAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACt                                                                       <  1:3794222/140‑1 (MQ=255)

                                                                      gcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCTAACCCTCACCCCAAAGGGGCGTGGGGACCGTTCAGTACGGAATTTTGGGTGACGGTGGTATCCAGCATTTAATACt                                                                       >  1:9164691/1‑140 (MQ=38)

                                                                      gcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACACCCAAACCCAAAACCCCAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTTTAGGTGCTAAACAGCATTataaaat                                                                       >  2:2112225/1‑134 (MQ=255)

                                                                       ccAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCTCACCCTAAACCTCAACCCAAAGGGGCGAGGGGTCCGGTCAGTTCGGAAATTAGCTTTAAGGTGGGATACAGCATTAAATACTg                                                                      >  2:3932657/1‑140 (MQ=255)

                                                                                  tcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCTAACCCTCACCCCCAAAGGGCGAGGGGACCCTTCAGTACGGAAATTAGCTTGACGGTGCTAACCACCAATAAATAATGGCAATActata                                                           >  2:5883656/1‑137 (MQ=255)

                                                                                         aTGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCaaa                                                    <  1:7069944/140‑1 (MQ=255)

                                                                                             tGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAAACCACTACCCAAATGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATACAGCATTAAATACAGGCAAAAATCACACGCaaaaaac                                                >  1:10031879/1‑137 (MQ=255)

                                                                                              ggCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCg                                               <  2:143635/140‑1 (MQ=255)

                                                                                                cgcgTTGAATTTTACATCCCGTACGTTACCCTCACCCTAACCCACTCCCCAAAGGGGCGAGGGGACCGGTCAGTACGGAATTTAGCTTTACGGTGCTATCCAGCATTAAATAAAGGCAATACTCTCACGCAAAATCCgca                                             >  2:7540583/1‑140 (MQ=39)

                                                                                                  cgTTGAATCTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCATAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCgcagc                                           <  1:9430372/140‑1 (MQ=255)

                                                                                                    ttGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCAc                                         <  2:61895/140‑1 (MQ=255)

                                                                                                    ttGAATTTTACATCCCGTACGTTCCCCTCACCCGAACCCTCTACCCCAAGGGGCGAGGGGCCCGTTAAGTAAGGAAGTTAGCTTGACGGTGCGATCCAGCATTAAATACGGGCAATACTCTCACGCAAAATCCGCAGCAc                                         >  2:2602825/1‑140 (MQ=255)

                                                                                                    ttGAATTTTACATCCCGTACGTTCCCCACACCCAAAACCTCAACCCAAAGGGGCGCGGGGGCCGGTAAGTACGGAAATTAGCGTGACGGTGCTATACAGCATTAAATAAAGGCAAAACAAAAAACCAAAATACGCAGCAc                                         >  1:830508/1‑140 (MQ=255)

                                                                                                    ctGAATTTTACCCCCCGTACGTTCCCCTCACCCGCACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGAACGGAATTTAGCAAGACGGTGCAATCCAGCATTAAATACTCGCAATACTCTCACGCAAAATCCGCAGCAc                                         <  2:6884660/139‑1 (MQ=37)

                                                                                                     tGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCAcc                                        <  1:8479352/140‑1 (MQ=255)

                                                                                                            tACATCCCGTACGTTCCCCACACCCAAACCCACACCCCCAACGGGCGTGGGGCCCGGTCAGTACGGAATTTATCATGACGGTGCTAACCAGCAATAAAAACAGGCAAGACTCTCACGCAAAATACGCAGCACCgacacat                                 >  2:8399888/1‑134 (MQ=255)

                                                                                                            tACATCCCGTACGTTACCCTCACCCTAAACCTCTCCCCAAATGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCAAGCATTAAATAATGGCAATACTCTCCCGCAAAATACGCAGCTCCGTCTCtt                                 >  2:9739207/1‑140 (MQ=255)

                                                                                                                        gtgccccTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACTGCTGCGAt                     <  2:5356191/137‑1 (MQ=255)

                                                                                                                        gTTCCCCTCACCCTAACCCTCTCCCCAACGGGGCGAGGGGACCGTTCAGTACGGAATATAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGAt                     <  1:7541774/140‑1 (MQ=255)

                                                                                                                        gTTCCCCTCACCCTAACCCTCTCCCCAAAGGTGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGAt                     <  1:7767686/140‑1 (MQ=255)

                                                                                                                        gTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGAt                     <  1:8743244/140‑1 (MQ=255)

                                                                                                                             ccTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCGGCGCTTCggg                >  2:8286065/1‑140 (MQ=255)

                                                                                                                              cTCACCCTAACCCTAACCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAAATTATCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACACACACGCAAAATCCGCACCACCCTCACATCACCGCAGCGAATCGGGt               >  2:10490390/1‑140 (MQ=37)

                                                                                                                              cTCACCCAAAACCACACCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAAATTAGCTTGACGGTTCTATCCAGCAATAAATACTGGCAAAACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGt               >  2:8784134/1‑140 (MQ=255)

                                                                                                                                   ccTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTATTg          >  1:5854622/1‑140 (MQ=255)

                                                                                                                                      aaCCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGa       >  2:2181160/1‑140 (MQ=255)

                                                                                                                                      aaCCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGa       >  1:8130965/1‑140 (MQ=255)

                                                                                                                                       aCCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGaa      <  1:6416102/140‑1 (MQ=255)

                                                                                                                                           tctcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGc  <  1:8953979/140‑1 (MQ=255)

                                                                                                                                           tctcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGc  >  1:1307625/1‑140 (MQ=255)

                                                                                                                                           tctcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGc  >  2:8434540/1‑140 (MQ=255)

                                                                                                                                           tctcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGc  >  2:6404253/1‑140 (MQ=255)

                                                                                                                                           tctcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGc  >  2:3049969/1‑140 (MQ=255)

                                                                                                                                           tctcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGc  >  1:5107252/1‑140 (MQ=255)

TCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGC  >  NZ_CP025268/3505420‑3505698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`