Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252683,505,5610TA21.5% 539.5 / 35.5 231intergenic (+92/+60)phoB/yhfSDNA‑binding transcriptional regulator FrlR/aminotransferase class I/II‑fold pyridoxal phosphate‑dependent enzyme
Reads supporting (aligned to +/- strand):  ref base T (59/120);  new base A (49/0);  total (110/121)
Fisher's exact test for biased strand distribution p-value = 7.24e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 301 total aligned reads displayed.

CCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCAC  >  NZ_CP025268/3505424‑3505700
                                                                                                                                         |                                                                                                                                           
    gcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTACCCCTCTCCCCaa                                                                                                                                       <  2:7699275/140‑1 (MQ=255)
     ccAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGTTGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACACTAAACCACTACCCaat                                                                                                                                      >  2:302037/1‑139 (MQ=39)
       actCGCATCACCTTCACTATTGATAAGAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAgg                                                                                                                                    <  2:8774343/138‑1 (MQ=255)
       aaTCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCaccgg                                                                                                                                    <  1:4722843/140‑5 (MQ=255)
            cATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGa                                                                                                                               <  1:10206476/140‑1 (MQ=255)
            cATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGa                                                                                                                               <  1:7571720/140‑1 (MQ=255)
                 ccTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGa                                                                                                                          <  2:6278192/140‑1 (MQ=255)
                 ccTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGa                                                                                                                          <  1:4296330/140‑1 (MQ=255)
                    tAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGGCCACACCCAAAACCACACCCCAAAAGGGGGAGGGGGccc                                                                                                                       >  1:2113648/1‑139 (MQ=37)
                     aaCTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCAAAACCACTCCCCCAAGGGGCGAGGGGACCgg                                                                                                                      >  1:6226986/1‑139 (MQ=38)
                      aCTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGtt                                                                                                                     <  1:3221671/140‑1 (MQ=255)
                          ttGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTcccc                                                                                                                                         <  1:9854445/116‑1 (MQ=255)
                           tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTACCCAAAGGGGCGCGGGGACCGTTCAGTa                                                                                                                >  1:7779426/1‑140 (MQ=255)
                           tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCAAACCCTCGCCCCCAAGGGGCGCGGGGACCGGTCAggg                                                                                                                >  1:10394647/1‑138 (MQ=38)
                           tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTGGCCCACAACCAAAACCTAACACCAAACGGGCGTGGGGGCCGTTGAGTa                                                                                                                >  1:8386776/1‑140 (MQ=25)
                           tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATACGGCGATGCTGGATCGTTGAATTTTACATCCCGTACGTTCCCCAAACCCCAACACACACCCCAAACGGGGGTGGGGAGCGTTCACTa                                                                                                                >  1:7721722/1‑140 (MQ=25)
                            gATAATAAAAGACATTAACCGTAGGCCTGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACACCCAAAACCACACCCCAAACGGGCGAGGGGACCGTTCAGTaa                                                                                                               >  2:5628215/1‑139 (MQ=38)
                            gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTAc                                                                                                               >  1:7054372/1‑140 (MQ=255)
                            gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAAACCTCTCCCCAAAGGGGCGAGGGGACCGTTAAGGAc                                                                                                               >  2:8152661/1‑140 (MQ=255)
                            gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCAAACCCACTCCCCAAAGGGGCGAGGGGACCGTTCAGTAc                                                                                                               >  2:827388/1‑140 (MQ=255)
                            gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCAACCTAACCCTCTCCCCCAAGGGGCGTGGGGACCGTTCAGTAc                                                                                                               >  2:10332481/1‑140 (MQ=255)
                                    aaGACATTAACCGTCGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCCAACCCAAAACCCCACCCCCAAAGGGCGAGGGGGCCCGTCAGTACGGAAtttt                                                                                                       >  1:3428682/1‑139 (MQ=25)
                                    aaGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAAACCACTCCCCAAAGGGGCGAGGGGACCGTTAAGTACGGAATTTa                                                                                                       >  2:203992/1‑140 (MQ=255)
                                    aaGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACCCCCAAAACCTCACCCCAAATGGGCGAGGGGACCCTTCAGTACGGAAATTa                                                                                                       >  1:10692834/1‑140 (MQ=38)
                                     aGACATGAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCTCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAg                                                                                                      <  1:8112771/140‑1 (MQ=255)
                                         aTTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTg                                                                                                  >  2:7278195/1‑140 (MQ=255)
                                          ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                                 <  1:2935429/140‑1 (MQ=255)
                                          ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                                 <  1:4631954/140‑1 (MQ=255)
                                          ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                                 <  2:7376754/140‑1 (MQ=255)
                                          tgaaCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGATGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                                 <  2:6905763/138‑1 (MQ=255)
                                            aaCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAACCCACTCCCCCAAGGGGGGAGGGGCCCGGTCAGTACGGCCTTTAGCTTGACg                                                                                               >  2:8953978/1‑140 (MQ=37)
                                             aCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACgg                                                                                              <  1:2618040/140‑1 (MQ=255)
                                               cGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                            <  2:2969665/140‑1 (MQ=255)
                                               cGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCCCAGGGGCGAGGGGACCGTTCAGTACGGATTTTAGCTTGACGGTg                                                                                            <  2:2748107/140‑1 (MQ=255)
                                                gTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAACCCTCTCCCCAAAGGGGCGAGGGGAACGTTCAGTACGGAATTTAGCTTGACGGTGc                                                                                           >  2:4739350/1‑140 (MQ=255)
                                                  aGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACACCCAAACCCACACCCCAAAAGGGCGCGGGGCCCCGTCAGTAAGGAATTTAGCTTGACGGTGCTa                                                                                         >  1:8723277/1‑140 (MQ=37)
                                                   ggCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAACCCACAACCCCAAAGGGCGAGGGGACCCGTAAGAACCGAAATTAGATTTACGGTGGTaa                                                                                        >  1:5597750/1‑139 (MQ=25)
                                                    gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATc                                                                                       <  2:1129065/140‑1 (MQ=255)
                                                      cGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCa                                                                                     <  1:8152662/140‑1 (MQ=255)
                                                         aTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTGCCCCCACCCAAACCCACTACCCCAACGGGGGAGGGGGCCCGTCAGTACGGAATTTAGCTTGACGGTGCTATACAGCa                                                                                  >  1:6395456/1‑140 (MQ=255)
                                                          tacgATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGATGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCCAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCAt                                                                                 <  2:6845514/137‑1 (MQ=255)
                                                           aaGATGCGCCAGCAACGCATCCGGCGATGCTGGCGCGTTGAAGTTTACATCCCGTACGTTCCCCCCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCAtt                                                                                <  2:3142657/140‑1 (MQ=255)
                                                              aTGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTACCCCAAAACCACACCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTaaa                                                                             >  2:9303791/1‑140 (MQ=255)
                                                               tGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTACCCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAAt                                                                            <  1:4103141/140‑1 (MQ=255)
                                                               tGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAAt                                                                            <  2:1667132/140‑1 (MQ=255)
                                                                gcgcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCTAAACCACACCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATa                                                                           >  2:8613691/1‑140 (MQ=255)
                                                                 cgcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATAc                                                                          <  1:3591467/140‑1 (MQ=255)
                                                                  gcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACACCCAAAACCCCTCCCCAAAGGGGCGAGGGGGCCCGTAAGTAAGGGATTTATATTGAGGGGGGTATACAGCATTaaaaaat                                                                         >  1:5297853/1‑136 (MQ=255)
                                                                  gcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCTAACCCTCACCCCAAAGGGGCGTGGGGACCGTTCAGTACGGAATTTTGGGTGACGGTGGTATCCAGCATTTAATACt                                                                         >  1:9164691/1‑140 (MQ=38)
                                                                   ccAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCATGACGGTGCTAAACAGCATTAAATACTg                                                                        >  2:6064416/1‑140 (MQ=255)
                                                                           gcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATAct                                                                <  2:2249518/140‑1 (MQ=255)
                                                                            catcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCACAGGGGCGAGGGGACCGGTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAAAAcac                                                               >  2:374142/1‑138 (MQ=255)
                                                                              tcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCTAACCCTCACCCCCAAAGGGCGAGGGGACCCTTCAGTACGGAAATTAGCTTGACGGTGCTAACCACCAATAAATAATGGCAATActata                                                             >  2:5883656/1‑137 (MQ=255)
                                                                                 ggCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTGGCCCACACCCAAACCCACACCCCAAAGGGGCGGGGGGGGCGGTGAGTACGGAATTTTGCTTGACGGTGCGATACAGCATTAAAAAATTGGAATActaaaaac                                                          >  1:8439909/1‑134 (MQ=255)
                                                                                     aTGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCaaa                                                      <  1:5863598/140‑1 (MQ=255)
                                                                                       gCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCGCACCCGAACCCACACCCCAAAGGGGCGAGGGGCCCGGTCAGTACGGGATGTAGCTTGACGGTGCGATACCCCCCTAAAAACAGGCCATACACTCACGccacaa                                                    >  1:9727242/1‑135 (MQ=255)
                                                                                         tGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATcc                                                  <  1:1699292/140‑1 (MQ=255)
                                                                                         tGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAAACCACTACCCAAATGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATACAGCATTAAATACAGGCAAAAATCACACGCaaaaaac                                                  >  1:10031879/1‑137 (MQ=255)
                                                                                          ggCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCg                                                 <  2:143635/140‑1 (MQ=255)
                                                                                           gcgcGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCgc                                                <  1:7437182/140‑1 (MQ=255)
                                                                                            cgcgTTGAATTTTACATCCCGTACGTTACCCTCACCCTAACCCACTCCCCAAAGGGGCGAGGGGACCGGTCAGTACGGAATTTAGCTTTACGGTGCTATCCAGCATTAAATAAAGGCAATACTCTCACGCAAAATCCgca                                               >  2:7540583/1‑140 (MQ=39)
                                                                                              cgTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCgcagc                                             <  1:6819643/140‑1 (MQ=255)
                                                                                              cgTTGAATCTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCATAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCgcagc                                             <  1:9430372/140‑1 (MQ=255)
                                                                                                ttGAATTTTACATCCCGTACGTTCCCCTCACCCGAACCCTCTACCCCAAGGGGCGAGGGGCCCGTTAAGTAAGGAAGTTAGCTTGACGGTGCGATCCAGCATTAAATACGGGCAATACTCTCACGCAAAATCCGCAGCAc                                           >  2:2602825/1‑140 (MQ=255)
                                                                                                        tACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAATGGGGTAGGGGACCGTTAAGTCCGGCAGTTAGCTTGACGGTGCGATGCAGCCTTAAATACTGGCACTAGACTCACGCAAAATACGCAGCACCGTCTCtt                                   >  2:9739215/1‑140 (MQ=37)
                                                                                                                    gtgccccTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACTGCTGCGAt                       <  2:5356191/137‑1 (MQ=255)
                                                                                                                    gTTCCCCTCACCCTAACCCTCTCCCCACAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGAt                       <  2:1327831/140‑1 (MQ=255)
                                                                                                                     ttCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGAtt                      <  1:10811963/140‑1 (MQ=255)
                                                                                                                          cTCACCCTAACCCTAACCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAAATTATCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACACACACGCAAAATCCGCACCACCCTCACATCACCGCAGCGAATCGGGt                 >  2:10490390/1‑140 (MQ=37)
                                                                                                                                  aaCCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGa         >  2:2181160/1‑140 (MQ=255)
                                                                                                                                  aaCCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGa         >  1:8131434/1‑140 (MQ=255)
                                                                                                                                  aaCCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGa         >  1:6960102/1‑140 (MQ=255)
                                                                                                                                  aaCCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGa         <  2:8379609/140‑1 (MQ=255)
                                                                                                                                       tctcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGc    >  2:8434540/1‑140 (MQ=255)
                                                                                                                                       tctcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGc    <  1:8953979/140‑1 (MQ=255)
                                                                                                                                       tctcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGc    >  1:5107252/1‑140 (MQ=255)
                                                                                                                                       tctcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGc    >  2:3049969/1‑140 (MQ=255)
                                                                                                                                       tctcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGc    >  2:6404253/1‑140 (MQ=255)
                                                                                                                                        ctcCCCAAAGGGGCGAGGGGACCGTTNAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGCNAATGCGAATCGca   <  1:5751239/140‑1 (MQ=255)
                                                                                                                                        ctcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGca   <  1:9900387/140‑1 (MQ=255)
                                                                                                                                        ctcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGca   <  1:1236010/140‑1 (MQ=255)
                                                                                                                                        ctcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGca   <  2:6226986/140‑1 (MQ=255)
                                                                                                                                        ctcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTACACCGCTGCGATTCGGGTTAATGCGAATCGca   >  1:6453481/1‑140 (MQ=255)
                                                                                                                                         tcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCa                                                                                     >  1:2238690/1‑57 (MQ=255)
                                                                                                                                         tcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCa                                                                                     <  2:4368385/57‑1 (MQ=255)
                                                                                                                                         tcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGGAATACTCTCACGCAAAATCCGCAGCACCGTCGCTTCACCGCTGCGAGTCGGGTTAATGCGCATCGcac  >  2:5281730/1‑140 (MQ=255)
                                                                                                                                         tcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAATATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac  >  1:6536525/1‑140 (MQ=255)
                                                                                                                                         tcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac  >  1:1441103/1‑140 (MQ=255)
                                                                                                                                         tcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac  >  2:4630577/1‑140 (MQ=255)
                                                                                                                                         tcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac  >  2:4514926/1‑140 (MQ=255)
                                                                                                                                         tcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac  >  1:182256/1‑140 (MQ=255)
                                                                                                                                         tcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac  >  2:4127927/1‑140 (MQ=255)
                                                                                                                                         tcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac  >  2:1876103/1‑140 (MQ=255)
                                                                                                                                         tcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac  >  1:4903978/1‑140 (MQ=255)
                                                                                                                                         tcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac  >  2:3341845/1‑140 (MQ=255)
                                                                                                                                         tcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac  >  1:5476565/1‑140 (MQ=255)
                                                                                                                                         tcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac  >  2:9600475/1‑140 (MQ=255)
                                                                                                                                         tcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGca   >  2:7913546/1‑139 (MQ=255)
                                                                                                                                         tcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGAGTCGGGTTAATGCGAATCGcac  >  2:6637361/1‑140 (MQ=255)
                                                                                                                                         tcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGAAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGcac  >  2:8921662/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                           
CCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCAC  >  NZ_CP025268/3505424‑3505700

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑