Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP025268107,3860AT22.1% 714.0 / 74.2 310intergenic (+81/‑150)lpxC/secMUDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase/secA translation cis‑regulator SecM
Reads supporting (aligned to +/- strand):  ref base A (52/188);  new base T (68/0);  total (121/189)
Fisher's exact test for biased strand distribution p-value = 1.49e-35
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 346 total aligned reads displayed.

TCCAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGC‑TGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCGGGA  >  NZ_CP025268/107247‑107525
                                                                                                                                            |                                                                                                                                            
 ccAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAACTCTGGCACTATCTCCGGCCAGGTGAACCAGTCT‑TTTTTTTTTGaa                                                                                                                                             <  2:1672262/140‑1 (MQ=255)
 ccAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTGaa                                                                                                                                             <  2:4192962/140‑1 (MQ=255)
     gacgacGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTGTAtttt                                                                                                                                         >  1:348068/1‑140 (MQ=255)
       cgacgCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTGAATTTtat                                                                                                                                       <  2:8969991/140‑1 (MQ=255)
       cgacgCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTGAATTTtat                                                                                                                                       <  2:8486450/140‑1 (MQ=255)
       cgacgCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTGAATTTtat                                                                                                                                       <  2:5205878/140‑1 (MQ=255)
          cgCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTTTATTTTTTaat                                                                                                                                    >  1:7385376/1‑139 (MQ=255)
          cgCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTTTATTTTATaat                                                                                                                                    >  1:7287584/1‑139 (MQ=255)
          cgCAGAACTGCCGTTGGCCTTCAAAGCGCATTCAGAAGTACTGGCATAACGACATTTATACTTTCGTATAAAATTCGACTGGCAAATCTGGCACTATCTCCGGCCAGTTGAACAAGTAG‑TTTTTGTTTGAAATTgattct                                                                                                                                    >  2:9580169/1‑137 (MQ=255)
            cAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTGAATTTTATAagag                                                                                                                                  <  1:9966528/140‑1 (MQ=255)
              taaCTGCGGTTGGCATTCAAAGCGCCTTCTGCTTTACTGGCATAACTACTTTTATACTGTCTTATAAAATTCGAGTGGAAAATCTGGCACTCTCTCCGGCCAGGTTAACAAGTCT‑TTTTTTTTTGAATTTTATAAGAGCt                                                                                                                                <  1:6039752/139‑1 (MQ=255)
                  tGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTGAATTTTATAAGAGCTATaa                                                                                                                            <  1:2892960/140‑1 (MQ=255)
                    ccGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTGTATTTTATAAGAGCAATaaaa                                                                                                                          >  2:5716927/1‑140 (MQ=255)
                    ccGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TGTTTTTTTTTTTTTTTTAATATCTATtaaa                                                                                                                          >  1:3857247/1‑140 (MQ=255)
                     cGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTAGTATAAAATTCGACTGTCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTGAATTTTATAAGAGCTATaaaaa                                                                                                                         <  2:536434/140‑1 (MQ=255)
                         ggCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTTTATTTTATAATAAATaaaaaaaaaag                                                                                                                     >  1:2725350/1‑137 (MQ=255)
                         ggCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTTAATTTTATAAGATCTATAAAAAAAgg                                                                                                                     >  2:7156001/1‑140 (MQ=255)
                         ggCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TGTTTTTTTGTATTTTATAATTGCTTAAAAAAACgg                                                                                                                     >  1:5573816/1‑140 (MQ=255)
                         ggCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TGTTTTTTTGAATTTTATAAAAGCAaaaaaaaaaag                                                                                                                     >  2:2892960/1‑137 (MQ=255)
                               cAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGNCCAGGTGAACCAGTCG‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGaa                                                                                                               <  1:5798955/140‑1 (MQ=255)
                               cAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGaa                                                                                                               <  2:1302904/140‑1 (MQ=255)
                                 aaGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACg                                                                                                             <  2:7008316/140‑1 (MQ=255)
                                        ttCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTTTAATTTTTAATATATAAAAAAAAAAGTTGGAAAGC‑GGtttt                                                                                                     >  1:3719602/1‑140 (MQ=255)
                                           aGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGC‑TGTTTTCtt                                                                                                  <  2:1402914/140‑1 (MQ=255)
                                                tACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGC‑TGTTTTCTTAAGCa                                                                                             <  1:1041576/140‑1 (MQ=255)
                                                tACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGC‑TGTTTTCTTAAGCa                                                                                             <  2:7233548/140‑1 (MQ=255)
                                                tACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGC‑TGTTTTCTTAAGCa                                                                                             <  2:8360850/140‑1 (MQ=255)
                                                 aCTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGACG‑TGTTTTTTTTTATTTTATAAAAGATAAAAAAAAAAGTTGGGAAGC‑TGTTTTGTTAAgtaa                                                                                            >  1:4177321/1‑137 (MQ=255)
                                                    ggCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGC‑TGTTTTCTTAAGCACttt                                                                                         <  1:1158454/140‑1 (MQ=255)
                                                    ggCATAACGACATTTATACTGTCGTATAAAATTCGACGGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGC‑TGTTTTCTTAAGCACtt                                                                                          <  1:6656605/140‑1 (MQ=255)
                                                     gCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTTTATTTTTTAATATAAAAAAAAAAAGGTGGGAACGC‑AGTTTTTTTTATCAcaatt                                                                                        >  1:2243762/1‑136 (MQ=255)
                                                     gCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTGTAATTTATAATAGCTATAAAAAACGGTGCGAACGC‑GGTTTTCTTAAGCACattt                                                                                        >  2:2681114/1‑140 (MQ=255)
                                                     gCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGC‑TGTTTTCTTAAGCACtttt                                                                                        <  2:3092665/140‑1 (MQ=255)
                                                       aTAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TGTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGC‑GGTTTTCTTAAGCACATTTca                                                                                      >  2:7945215/1‑139 (MQ=255)
                                                        tAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGC‑TGTTTTCTTAAGCACTTTTCCg                                                                                     <  2:3528981/140‑1 (MQ=255)
                                                         aaCGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGC‑TGTTTTCTTAAGCACTTTTCCGc                                                                                    <  1:441478/140‑1 (MQ=255)
                                                          aCGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTTTATTTTTTAATTTCTATAAAAAAAGGTTCGTACGC‑TGTTTTATTTATCACTTTTACGcc                                                                                   >  2:5480271/1‑139 (MQ=255)
                                                          aCGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTTTATTTTTTAATTTATATAAAAAAAGGTGGGGACGC‑GGTTTTATTTTGGCCTTTTTCGca                                                                                   >  1:7944236/1‑140 (MQ=255)
                                                          aCGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTTTATTTTATAATATCTATAAAAAAAGGTTCGAACGC‑GGTTTTATTTATCACttttaagca                                                                                   >  1:9127148/1‑135 (MQ=255)
                                                          aCGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTGAATTTtata                                                                                                                                      <  1:8730201/90‑1 (MQ=255)
                                                          aCGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTGAATTTTATAATAGCAATAAAAAACGGTGCGAACGC‑TGTTTTCTTAATCACTTTTACGca                                                                                   >  2:6305035/1‑140 (MQ=255)
                                                           cGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGACCCAGTCG‑TATTTTTTTGAAGTTTATAAGAGCTATAAAAAACGGTGCGAACGC‑TGTTTTCTTAAGCACTTTTCCGcac                                                                                  <  2:6193575/140‑1 (MQ=255)
                                                           cGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGC‑TGTTTTCTTAAGCACTTTTCCGcac                                                                                  <  2:8450535/140‑1 (MQ=255)
                                                           cGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGC‑TGTTTTCTTAAGCACTTTTCCGcac                                                                                  <  1:2635261/140‑1 (MQ=255)
                                                               aTTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTTTTTTTTTTATTTGGTTTTAAAAAAGGGGGGGACGC‑GGTTTTATTTAGGACATTTACGCACaaag                                                                              >  2:473052/1‑138 (MQ=255)
                                                               aTTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTTTATTTTATAAAAGCTATAAAAAACTGTTCGAACGC‑GGTTTTTTTAAGAACAATTAAGCACAAca                                                                              >  2:2355366/1‑139 (MQ=255)
                                                               aTTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTGAATTTTATAATAGCTATAAAAAACGGTGCGAACGC‑AGTTTTCTTAAGCACAATTACGCACaaaa                                                                              >  2:8046975/1‑138 (MQ=255)
                                                                     aCTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGC‑TGTTTTCTTAAGCACTTTTCCGCACAACTTATCtt                                                                        <  1:6440783/140‑1 (MQ=255)
                                                                        gTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGC‑TGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCAt                                                                     <  1:1522187/140‑1 (MQ=255)
                                                                        gTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGC‑TGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCAt                                                                     <  2:1113463/140‑1 (MQ=255)
                                                                        gTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGC‑TGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCAt                                                                     <  2:6035576/140‑1 (MQ=255)
                                                                        gTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGC‑TGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCAt                                                                     <  1:1408108/140‑1 (MQ=255)
                                                                         tCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTGTATTTTATAATATCTATAAAAAACGGTGCGAACGC‑AGTTTTATTAAGCACATTTACGCACAACATATCttaaat                                                                    >  2:3452687/1‑136 (MQ=255)
                                                                         tCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTGAATTTTTTTATATCTTTAAAAAAAGGTGGGTACGC‑TGGTTTTTTTAGCACATTTGCGCACAACATAACTTctat                                                                    >  2:5878660/1‑137 (MQ=255)
                                                                         tCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TGTTTTTTTTCATTTTTTTAGAGCAATAAAAAAAGGTGCGAACGC‑GGTTTTATTAATCACATTTACGCAACACGTATATTCAtt                                                                    >  2:4382202/1‑140 (MQ=255)
                                                                          cGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTGTTTTTGAATTTTATAAGAGCGATAAAAAAACGTGCGAACGC‑AGTTTTCTAAAGCACTTTTTCCCACAAAGTAACTTCaagg                                                                   >  2:6473798/1‑137 (MQ=255)
                                                                           gTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TGTTTTTTTGAATTGTATAAGATCTAGAAAAAACGGGGGGAACGCGTGTTTT‑TTAAGAACATTTACGCCCACCATATCGTCAttag                                                                  >  1:4764582/1‑138 (MQ=255)
                                                                            tataAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGACCCAGTCG‑TGTTTTTTTTAAGTTTATAAGAGCTATAAAAAAACGTGCGAACCC‑TGTGTTGTTAAAAACATTTACGCACAAAATATATTCATTAGt                                                                 >  2:6795901/1‑140 (MQ=255)
                                                                             ataAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTGAATTTTATAAGATCTATAAAAAAAGGTGCGAACGC‑TGTTTTATTAAGCACTTTTACGCAAAACATATATTAATTAGTg                                                                >  2:5436848/1‑140 (MQ=255)
                                                                               acaaTCCGACCGGCACATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTGAATTTGATAAGCGCTATAAAAAACGGTGCGAACGC‑TGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCt                                                              <  1:7695735/138‑1 (MQ=255)
                                                                                  aTTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGC‑TGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCtgtg                                                           <  1:9368807/140‑1 (MQ=255)
                                                                                  aTTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGC‑TGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCtgtg                                                           <  1:1661155/140‑1 (MQ=255)
                                                                                   ttCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTGTATTTTATAAGAGCAATAAAAAAAGGGGCGAACGC‑GGTTTTCTTAATCACTTTTACGCCCACCATATCTTAAATCGTGCTGTgg                                                          >  2:3757498/1‑140 (MQ=255)
                                                                                   ttCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGC‑TGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTgg                                                          <  1:10001016/140‑1 (MQ=255)
                                                                                      gACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTTAATTTTATAATATCTATAAAAAAAGGTGCGAACGC‑TGTTTTATTAATCACATTTACGCACAAATTATAATAATTAGTGCTGTGGGCt                                                       >  2:8784762/1‑140 (MQ=255)
                                                                                        cTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGC‑TGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGc                                                     <  2:9098843/140‑1 (MQ=255)
                                                                                        cTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGC‑TGTTTTCTTAAGCAATTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGc                                                     <  2:1907746/140‑1 (MQ=255)
                                                                                                tCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGC‑TGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTa                                             <  1:5355020/140‑1 (MQ=255)
                                                                                                tCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGC‑TGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTa                                             <  1:7573155/140‑1 (MQ=255)
                                                                                                tCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGC‑TGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTa                                             <  2:947213/140‑1 (MQ=255)
                                                                                                tCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGC‑TGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTa                                             <  1:3422862/140‑1 (MQ=255)
                                                                                                          tctcCGGCCAGGTGAACCAGTCG‑TTTTTTTTTTTATTTTATAAGAGCTATAAAAAAAGGTGCGAACGC‑TGTTTTCTTAAGCACTTTTCCGCACAACTTATATTCAATAGTGGTGTGGGCTGCAGGCTTTAATGATAAgct                                   >  2:9133270/1‑138 (MQ=255)
                                                                                                              cGGCCAGGTGAACCAGTCG‑TGTTTTTTTTTATTTTTTAATAGCGATAAAAAAACGTGCGTACGC‑GGGTTTTTTAAACAAATTTTCCCACAAAATATATTAAATAGTGGGGTGGTCTGCAGGCATTAATGATAATAttttt                               >  2:10485887/1‑138 (MQ=255)
                                                                                                               ggCCAGGTGAACCAGTCG‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGC‑TGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTtgtg                              <  1:3259754/140‑1 (MQ=255)
                                                                                                                 ccAGGTGAACCAGTCG‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGC‑TGTTTTCTTAAGCACTTTTCCGCACATCTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTgcg                            <  2:496743/140‑1 (MQ=255)
                                                                                                                 ccAGGTGAACCAGTCG‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGC‑TGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTgcg                            <  2:7903868/140‑1 (MQ=255)
                                                                                                                 ccAGGTGAACCAGTCG‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGC‑TGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTgcg                            <  2:751240/140‑1 (MQ=255)
                                                                                                                 ccAGGTGAACCAGTCG‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGC‑TGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTgcg                            <  2:4265235/140‑1 (MQ=255)
                                                                                                                 cagggTGAACCAGTCG‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGC‑TGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTgcg                            <  1:8032993/137‑1 (MQ=255)
                                                                                                                    ggTGAACCAGTCG‑TTTTTTTTTGAATTTTTTTAAATGTATAAAAAAAAGTGGGGAAGC‑TTTTTTATTAATCAAAATTAAGCACAAAAAATATTAATTAGTGGTTGGGTCAGCAGGCTTTAATTATAATATTtttggggga                         >  2:3228417/1‑135 (MQ=255)
                                                                                                                      tGAACCAGTCG‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGC‑TGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTaaa                       <  2:2539256/140‑1 (MQ=255)
                                                                                                                           cAGTCG‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGC‑TGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGt                  <  1:881230/140‑1 (MQ=255)
                                                                                                                           cAGTCG‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGC‑TGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGt                  <  1:10496319/140‑1 (MQ=255)
                                                                                                                             gTCG‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGC‑TGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGttt                <  2:3972711/140‑1 (MQ=255)
                                                                                                                             gTCG‑TTTTATTTTGAATATTATAAGAGCTATAAAAAACGGTGCGAACGC‑TGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGttt                <  1:8425859/140‑1 (MQ=255)
                                                                                                                              tCG‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGC‑TGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTg               <  1:4935108/140‑1 (MQ=255)
                                                                                                                                  tttttttttGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGC‑TGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAat            <  1:1233719/140‑1 (MQ=255)
                                                                                                                                   ttttttttGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGC‑TGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAata           <  2:132727/140‑1 (MQ=255)
                                                                                                                                       ttttGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGC‑TGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGAt       <  2:10687351/140‑1 (MQ=255)
                                                                                                                                       ttttGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGC‑TGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGAt       <  2:2725442/140‑1 (MQ=255)
                                                                                                                                       ttttGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGC‑TGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGAt       <  2:4538589/140‑1 (MQ=255)
                                                                                                                                       ttttGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGC‑TGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGAt       <  2:229478/140‑1 (MQ=255)
                                                                                                                                        tttGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGC‑TGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATc      <  2:1803364/140‑1 (MQ=255)
                                                                                                                                            aaTTTTATAAGAGCTATAAAAAACGGTGCGAACGC‑TGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCGGGa  <  1:5436848/140‑1 (MQ=255)
                                                                                                                                            aaTTTTATAAGAGCTATAAAAAACGGTGCGAACGC‑TGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCGGGa  <  1:6983934/140‑1 (MQ=255)
                                                                                                                                            aaTTTTATAAGAGCTATAAAAAACGGTGCGAACGC‑TGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCGGGa  <  1:7156002/140‑1 (MQ=255)
                                                                                                                                            aaTTTTATAAGAGCTATAAAAAACGGTGCGAACGC‑TGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCGGGa  <  1:8996327/140‑1 (MQ=255)
                                                                                                                                            aaTTTTATAAGAGCTATAAAAAACGGTGCGAACGC‑TGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCGGGa  <  1:4332779/140‑1 (MQ=255)
                                                                                                                                            aaTTTTATAAGAGCTATAAAAAACGGTGCGAACGC‑TGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCGGGa  <  1:193514/140‑1 (MQ=255)
                                                                                                                                            aaTTTTATAAGAGCTATAAAAAACGGTGCGAACGC‑TGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCGGGa  <  1:2416910/140‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
TCCAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGC‑TGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCGGGA  >  NZ_CP025268/107247‑107525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑