Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP025268164,3950AT23.4% 294.9 / 28.3 161intergenic (+30/‑166)hrpB/mrcBATP‑dependent helicase HrpB/bifunctional glycosyl transferase/transpeptidase
Reads supporting (aligned to +/- strand):  ref base A (52/66);  new base T (0/36);  total (52/109)
Fisher's exact test for biased strand distribution p-value = 3.35e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 174 total aligned reads displayed.

GAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTT  >  NZ_CP025268/164256‑164533
                                                                                                                                            |                                                                                                                                          
gAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGgaga                                                                                                                                            <  2:6687524/140‑1 (MQ=255)
gAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGgaga                                                                                                                                            >  1:3244482/1‑140 (MQ=255)
   cGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAATCATGTCTGGCCGGACGACCCGGCAAATACTGCACCGTCGCGACGGACGACAAAGTAGTCGTACGGTGGGTGTG‑CTCCCTCTCCGGGGGGGTGAggg                                                                                                                                         >  2:10286729/1‑140 (MQ=255)
   cGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAggg                                                                                                                                         <  2:1551127/140‑1 (MQ=255)
   cGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAggg                                                                                                                                         <  2:1550984/140‑1 (MQ=255)
   cGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAggg                                                                                                                                         >  1:8212422/1‑140 (MQ=255)
   cGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAggg                                                                                                                                         <  1:5314051/140‑1 (MQ=255)
   cGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATGCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAggg                                                                                                                                         <  2:2619181/140‑1 (MQ=255)
    gaaCCGTGAGGTGCAACAAGAGATGAACGGGCGGTATCCCAAACCTGTCTGGCCGGACGACCCGCAAAATACTGCACCGCCGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCGGTGGGAGAGGGc                                                                                                                                        <  2:9425059/138‑1 (MQ=255)
    gTACCGTGAGGTGCACAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAACTGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGc                                                                                                                                        <  1:9123323/140‑1 (MQ=255)
    gTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGc                                                                                                                                        <  1:389775/140‑1 (MQ=255)
     tACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGcc                                                                                                                                       <  1:10385489/140‑1 (MQ=255)
     tACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGcc                                                                                                                                       <  1:9509857/140‑1 (MQ=255)
     tACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGcc                                                                                                                                       <  2:8786051/140‑1 (MQ=255)
     tACCGTGAGGTGCAAAAAGACATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGCCGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGcc                                                                                                                                       <  2:4981216/140‑1 (MQ=255)
       ccGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCg                                                                                                                                      >  1:7868810/1‑139 (MQ=255)
        cGTGAGGTGCAAAAAGAGATTATAGGGCGTTATCCCAAACATGTCTGGCCGGACCACCCGGCAAATACTGCCCCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGGGGGAGAGGGCCggg                                                                                                                                    <  2:5834620/140‑1 (MQ=255)
        cGTGAGGTGCAAAAAGAGATGAAAGGGCGTAATCCCAAACATGTCTGGCCGGACGACCCGGCAATTACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCggg                                                                                                                                    <  2:7372378/140‑1 (MQ=255)
            aGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGa                                                                                                                                >  1:1532663/1‑140 (MQ=255)
             ggTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAg                                                                                                                               >  1:8126003/1‑140 (MQ=255)
               tGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAggg                                                                                                                             >  2:3783727/1‑140 (MQ=255)
               tGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAggg                                                                                                                             >  1:8277709/1‑140 (MQ=255)
                 cAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCa                                                                                                                           >  1:117314/1‑140 (MQ=255)
                         taTGAATGGGGGTTATCCCAAACTTGTGTGGCCGGACGACCCGGCAATTACTGCACCGAGGCGACGGACTAAAATGTTTTCGTATGGTTGGTTTT‑CTCCCTCGCCCTGTGGGAGTGGGCCGGGGTGTGGGCATCAgcgcg                                                                                                                   <  2:1246256/139‑1 (MQ=255)
                         taTGAAAGGGCGTTAGCCAAAACTTGTCTGTCCGGACGACCCGGCAAATACGGCACCGACGCTACGGACGAACATTTTTTCGTATTGTTGTTTTT‑CTCCCTATCCCTTGGGGAGAGGGCCGGGTTGTGGGCCTCAgcgcg                                                                                                                   <  2:1879192/139‑1 (MQ=255)
                         gctGAAAGGGCGTTACCCCAAACTGGTCTGGCCGGACGCCCCGGCAATTACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAgcgcg                                                                                                                   <  2:7041868/138‑1 (MQ=38)
                         gaTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAgcgcg                                                                                                                   >  1:7012717/1‑140 (MQ=255)
                         gaTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAgcgcg                                                                                                                   >  2:5591362/1‑140 (MQ=255)
                         gaTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAgcgcg                                                                                                                   >  2:6965939/1‑140 (MQ=255)
                         gaTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAgcgcg                                                                                                                   >  1:9003015/1‑140 (MQ=255)
                         gaTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGAAGAAACAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCACTGTGGGAGAGGGCAGGGGTGAGGGCATCAgcgag                                                                                                                   >  2:7877378/1‑138 (MQ=39)
                              aaGGGCGTTATCCAAAACATGTCTGGCCGGACGACCCGGCAATTACTGCCCCGCCGCGCCGGACGAAAAAGTTTTCTTACGTTTGGTTTT‑CTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGt                                                                                                              <  2:483798/140‑1 (MQ=25)
                               tggggGTTATCCCAAACTTGTCTGGCCGGACGACCCGGCAATTCCTGCCCCGACGCGAGGGACGAAAAAGTATTCGTACGGTGGGTTTT‑CTCCCTCTCCCGGGGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGtt                                                                                                             <  2:7359941/139‑1 (MQ=37)
                               tgggCGTTACCCCAAACTGGTGGGGCCGGACGCCCCGGAAAATATGGCCCCGACGCTAGGGAGTAAAATTTATTGTTATGGTTGGTTTT‑CTCCCTGACCGTGTGGGTTTGGGCGGGGGTGAGGGCATCAGCGCGCACGtt                                                                                                             <  2:9204279/139‑1 (MQ=255)
                               aGGGCGTTATCCCAAACTTGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGTACTAAAAATTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGTTAGGGCCGGGGTGAGGGCATCAGCGCGCACGtt                                                                                                             <  2:7190983/140‑1 (MQ=38)
                               aGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGAAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGtt                                                                                                             <  2:10246040/140‑1 (MQ=255)
                                gggCGTTATCCCAACCATGTCTGGCGGGACGACCGGGCAAATACTGCACCGACGGGTGGTACGAAAATGTATTGGTAAGGTTGTTTTT‑GTCCCTCTCCGTGTGGGTGTGGGCGGGGGTGCGGGCATCAGCGCGCACGTtc                                                                                                            <  2:7573889/140‑1 (MQ=255)
                                gggCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACgggc                                                                                                            >  2:323609/1‑137 (MQ=255)
                                    gTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTtcaccc                                                                                                        <  2:10586433/140‑1 (MQ=255)
                                     ttATCCCAACCATGACTGGCCGCACGACCCCGCAACTCCGGCCCCGCCGCGACGGCAGAAAAAGTATTCTTAAGGTTGGTTTT‑CTCCCTCGCCCTGTGGGAGAGGGCCGGGGTGCGGGCATCAGCGCGCACGGtcaccct                                                                                                       <  2:7613016/140‑1 (MQ=25)
                                     ttATCCCAAATTTGTGGGGCGGGACCACCCGCAAAATAGGCCACCGACGCGAGTTACTAAAATTTTTTTGTATGTTTTTTTTT‑CACCCTCTCCGTGGGGTTGTGGGCGGGGGTGGGGCCCTCAGCGCGCACGTtcaccct                                                                                                       <  2:3884762/140‑1 (MQ=255)
                                     ttATCCCAAACATGTCGGGCCGGACGACCCGGCAATAACTGCACCGACGCTACGGACTAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCAACTCCCTGGGGGAGTGGGCCGGGGTGAGGGAATCAGCGCGCACGTtcaccct                                                                                                       <  2:685221/140‑1 (MQ=255)
                                     ttATCCCAAACAGGTCGGGCCGGACCCCCCGGCAAAACCTGCACCGACGCCACGGACGAAACAGTATTCGTACGGTTGGTGTT‑CACCCTCTCCCTGTGGGTGAGGGCCGGGGTGTGGGCATCAGCGCGCACGTtcaccct                                                                                                       <  2:2818815/140‑1 (MQ=255)
                                     ttAACCCAAACTTTTCGGGCGGGCCGACCGGGAAATTAATGCACCGACGCGTCGTACTAATATTTTTTCTTATGGTTGGTTTT‑CTCCCTCCCCGTGGGGTAGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTtcaccct                                                                                                       <  2:2518157/140‑1 (MQ=255)
                                     ttAACCCAAACATGTCGGGCCGCACCCCCCGGCAACTCCGCCACCGACGCGCCGCACTAAAAATTATTCGACCGGTGGGTTTA‑CCCCCACGCCCTGGGGGCGAGGGCCGGGGTGCGGCCCTCAGCGCGCACGTtcaccct                                                                                                       <  1:2669397/140‑1 (MQ=255)
                                     ttAACCAAAACTTGTCGGGCCGGACGCCCCGGAAATTACTGCACCGACGCGAGGGACTAAAATTTATTGGTATGTTGGGTTTT‑CTCCCTCTCCCGGGGGGTGTGGGCGGGGGTGAGGGCCTCAGCGCGCACGTtcaccct                                                                                                       <  1:7183536/140‑1 (MQ=255)
                                       aTCCAAAACTGGTCTGCCCGGACCCCCCGCCAAATACGCCACCGCCCCGCCGCACTAAAACTTTTTCTTACGGTTGGTGTA‑CACCCACACCCTGTGGTTGCGGGCGGGGGTGTGGGCCTCAGCGCGCACGTtcaccctcc                                                                                                     <  2:2027502/140‑2 (MQ=255)
                                            aaaCATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGTtcaccctcacccac                                                                                                >  2:8477254/1‑138 (MQ=255)
                                                aggTCGGGCCGTACGCCCCGCCAAATACTCCACCTACGCGCCGGACGAAAAATTTTTCTTATGGTTGGTTTT‑CTCCCACTCCATGTGGCTGTGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAAccc                                                                                            <  2:1083908/138‑1 (MQ=255)
                                                 tGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACTAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCct                                                                                           <  2:4548214/140‑1 (MQ=255)
                                                 gggcggcccGCACGCCCGGCCAAATACGCCACCGACGCGAGGGACGAAAATGAATTCTTACGGTGGGTTTC‑CACCCTCCCCCTGTGGGAGAGGCCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCct                                                                                           <  2:5174121/133‑1 (MQ=255)
                                                  gtgtGGCCGTACCCCCCGCAAAATCCGCCACCGACGCGACGGACTAAAATGTTTGCGTTTGTTTGGTTTC‑CGCCGCCTCCGGGGGGGTGTGGGCGGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCctc                                                                                          <  1:5640245/137‑1 (MQ=255)
                                                  gTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAAcccac                                                                                          >  2:10566836/1‑138 (MQ=255)
                                                  gTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGTGACCCACACCCAAAaccac                                                                                          >  1:6918502/1‑138 (MQ=37)
                                                  gTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGGCGCCCTCACCCGAAcccac                                                                                          >  1:6265207/1‑138 (MQ=38)
                                                  gTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAGCAGCGCGCACGTGCACCCACCCCCAAAcccac                                                                                          >  2:52156/1‑138 (MQ=38)
                                                  gTCGGGCCGGACGCCCCGGAAAATCCTGCACCCACGCGACGGACCAAAATTTATTCTTACGGTTGTTTTA‑CCCCCACCCCCTGTGGGTGTGGCCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCctc                                                                                          <  1:10831801/140‑1 (MQ=255)
                                                    cgggCCGGACGCCCCGGCACAGACTGCACCGACGCGACGGACGAACAAGTATTCGTAAGGTTGGTGTT‑CTCCCTCTCCCTGGGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCtctc                                                                                        <  2:6128643/138‑1 (MQ=38)
                                                       gccggccgCCCCGCCAAACACCGCACCGACGCGCCGGCCTAAAATGTATTCGTAAGGTTGGTTTT‑CGCCCTCCCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCt                                                                                     <  1:9867906/140‑1 (MQ=25)
                                                       gccggccgACCCGGCAAATCCCCCCCCGACGCGCCGGACGAAAAATTTTTCGAAAGGTTGGTTTC‑CACCCTCTCCCTGGGGGTGTGGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCt                                                                                     <  1:6543539/140‑1 (MQ=255)
                                                           gacgacCCGGCAAATACGGCACCGCCGCGCCGGACGAAAAAGTTTTCGTAAGGTTGGTGTT‑CGCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAg                                                                                 <  2:9606001/140‑1 (MQ=38)
                                                                 ccGGCAAATACTGCACCGACGCGACGGACGAAAAAGTTTTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGagag                                                                           <  2:7782208/140‑1 (MQ=255)
                                                                   ggaaaaTACTCCACCGACGCGAGGTACCAAAATGTATTCGTAGGGTTGGTTTA‑CCCCCTCTCCCTGTGGGTGCGGGCGGGGGTGGGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAggg                                                                         <  1:901343/137‑1 (MQ=255)
                                                                              cACCGACGCGACGGACGAAAAATTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGTAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAg                                                              <  2:5288245/140‑1 (MQ=255)
                                                                              cACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGTTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAg                                                              <  2:2453807/140‑1 (MQ=255)
                                                                              cACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAGCCGCGCGCACGTGCACCCGCACCCGAACCCACTCCCGCAAGGGAGAGGGGACCGATagag                                                              >  2:4432342/1‑140 (MQ=37)
                                                                              cACCGACCCGACGGACTAAAAATTATTCTTATGTTTGTTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAg                                                              <  1:5933365/140‑1 (MQ=37)
                                                                                ccGACGCTACGGACTAAATTGTTTTCTTATGTTTGGTTTT‑CGCCCTCTCCCTGTGGGATTGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGca                                                            <  1:8857671/140‑1 (MQ=37)
                                                                                ccGACGCGACGTACGAAAATGTATTCGAAAGGTTGGTTTT‑CACCCACACCCGGGGGGAGTGGGCCGGGGTGTGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGca                                                            <  1:10540733/140‑1 (MQ=37)
                                                                                   acgcgacgGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCCCGGGGACCCTCACCCGAAACCGCGCCCGCGAGGGTGAGGGGTACGATCGAggcccc                                                         >  2:9246761/1‑135 (MQ=21)
                                                                                   acgcgacgGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGACCCACAACCAAAACCACTACCACAAGGGGGTGGGGACCGCTAGAGcacca                                                         >  1:9739880/1‑138 (MQ=21)
                                                                                   acgcgacgGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCGCACCCAAACCCTCTCCCACAAGGGAGAGGGGACCGATCGAGCACaa                                                         >  2:8065899/1‑140 (MQ=255)
                                                                                   acgcgacgGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGGGCCCCGCCCCCGAACCCACTGCCGCGAGGGGGGGGGGGCCGCTCGAGcgcca                                                         >  2:3310090/1‑136 (MQ=18)
                                                                                        acggacgAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCACTCCCTCAAGGGAGAGGGGACCGATCGAGCCCAAAattt                                                    >  2:6028765/1‑140 (MQ=255)
                                                                                         cggacgAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTGGTCCCACCCCCCAAACCACAACCACCAGGGCGAGGGGGCCGCTCGAGcccacaaattg                                                   >  2:1038269/1‑133 (MQ=16)
                                                                                         cggacgAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGTTCACCCTCACCCTAACCCACTCCCTCAACGGAGAGGGGACCGATCGAGCACAAAttttt                                                   >  2:6657592/1‑139 (MQ=255)
                                                                                          ggccgAAATAGTATTCTTAAGGTTGGTTTT‑CTCCCTCTCCCTGGGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga                                                  <  2:8412333/137‑1 (MQ=255)
                                                                                           gacaAAAAATTATCCTTATGTTTGTTTAC‑CCCCCTCTCCCTGTGGAATCGGGCGGGGGTGGGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgag                                                 <  1:8351772/140‑1 (MQ=25)
                                                                                             ctaaaaaGTATTCGTAAGGTTGTTTTT‑CACCCTCTCCCTGTGGGAGTGGTCCGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGATCACAAATTTTgagag                                               <  2:531724/138‑1 (MQ=38)
                                                                                             cgAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgagag                                               <  2:9045701/140‑1 (MQ=255)
                                                                                               aaaaaGTTTTCTTATGTTTGTTTTT‑CACCCACGCCCTGTGGTATAGGGCCGGGGTGAGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGat                                             <  1:795066/140‑1 (MQ=37)
                                                                                               aaaaaGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGat                                             <  2:3684892/140‑1 (MQ=255)
                                                                                                 aatgTATTCGTATGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGTGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGatat                                           <  2:6574749/137‑1 (MQ=38)
                                                                                                      aTTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGTGCACCCTCCCCCAATCCCACAACCTCCAGGGCGGGGGGACCGATCGCGCCCACAATTTGTGAGATAacttct                                      >  2:10640626/1‑140 (MQ=18)
                                                                                                      aTTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCCCGTTGACCCCAACCCCAAACCACACCCCCCAAGGAGTGGGGCCCCCGGGAGCACAAAATTTTAGAGATAtcttat                                      >  1:2634129/1‑138 (MQ=16)
                                                                                                       ttCGTAAGGTTGGTTTT‑CTCCCTCTCCCGGTGGGAGTGGGCCGGGGTGAGGGCCTCAGCGCGCACGGTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttctt                                     <  2:4229435/140‑1 (MQ=255)
                                                                                                        tCGTATGGTTGTTTTA‑CCCCCTCTCCCTGTGGTCGTGGGCCGGGGTGAGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttcttc                                    <  1:7721434/140‑1 (MQ=38)
                                                                                                        tCGTAAGTTTGTTTTT‑CTCCCTCTCCGTGTGGGTGTGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttcttc                                    <  1:9443210/140‑1 (MQ=39)
                                                                                                        tCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttcttc                                    <  1:9922719/140‑1 (MQ=255)
                                                                                                        tCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttcttc                                    <  2:4636325/140‑1 (MQ=255)
                                                                                                        gcgtcgggttggttAC‑CCCCCACCCCCTGGGGGTGCGGCCGGGGGTGCGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttcttc                                    <  1:2969326/134‑1 (MQ=25)
                                                                                                           tAAGGTTGGTTTT‑CTCCCTCACCCTGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGt                                 <  1:4778446/140‑1 (MQ=255)
                                                                                                                 tggttTTCCCCCCTCTCCCTGGGGGAGTGGGCGGGGGTGGGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGt                            <  1:4221158/140‑1 (MQ=38)
                                                                                                                        tccccctccccctGTGGGTGTGGGCGGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaa                     <  1:2102230/137‑1 (MQ=39)
                                                                                                                          tccctctccctGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTGCACCCTCACCCAAACCCTCTCCCTCCAGGGAGAGGGGCCCGAGCGAGCACAAATTTTGAGAGATATATTATTATGTCTTGTAACagaaga                   >  2:8187982/1‑140 (MQ=37)
                                                                                                                            cctctcccgGTGGGAGTGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAAc                 <  1:10269706/140‑1 (MQ=255)
                                                                                                                                    tgtgGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGTTCACCCACACCCTAACCCTCTCCCGCAAGGGAGAGGGGTCCGATCGAGCACAAATTTTGTGAGATATATTATTCTGTGTTGTAAAAGAAGAACAGAAAATc         >  2:9814513/1‑140 (MQ=255)
                                                                                                                                          agagGGCCGGGGTGAGGGCCTCAGCGCGCACGGTCACCCTCCCCCAAACCCACTCCCACCAGGGAGCGGGGACCCAGAGAGCCCAAAATTTGAGGGATATCTTCTTATGTCTTGGTACAGCATAAAACAAAAAAGGGCtt   >  2:8953888/1‑140 (MQ=255)
                                                                                                                                          agagGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGGCCGGTCGAGCACAAATTTTGAGAGATATCTTCTTCTGTGGTGTAACAGTAGAACAGAAAATCGGGcgt   >  2:9921225/1‑138 (MQ=255)
                                                                                                                                           gagGGCCGGGGTGACGGCATCAGCGCGCACGTTCACCCTCACCCTACCCCTCTCCCTCAAGTGAGAGGGGACCGCTCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCttt  <  2:7764911/140‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                          
GAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTT  >  NZ_CP025268/164256‑164533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑