Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP025268164,4000CG21.3% 326.6 / 36.4 152intergenic (+35/‑161)hrpB/mrcBATP‑dependent helicase HrpB/bifunctional glycosyl transferase/transpeptidase
Reads supporting (aligned to +/- strand):  ref base C (52/66);  new base G (0/32);  total (54/98)
Fisher's exact test for biased strand distribution p-value = 1.57e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 161 total aligned reads displayed.

TACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAA‑CAGAAAATCGGGCTTTTGCGC  >  NZ_CP025268/164261‑164538
                                                                                                                                             |                                                                                                                                           
tACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGcc                                                                                                                                             <  1:10385489/140‑1 (MQ=255)
tACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGcc                                                                                                                                             >  1:9463851/1‑140 (MQ=255)
tACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTA‑CTCCCTCTCCCTGTGGGAGAGGGcc                                                                                                                                             <  2:7482743/140‑1 (MQ=255)
tACCGTGAGGTGCAAAAAGACATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGCCGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGcc                                                                                                                                             <  2:4981216/140‑1 (MQ=255)
  ccGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCg                                                                                                                                            >  1:7868810/1‑139 (MQ=255)
  ccGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCg                                                                                                                                            <  2:7868809/139‑1 (MQ=255)
   cGTGAGGTGCAAAAAGAGATTATAGGGCGTTATCCCAAACATGTCTGGCCGGACCACCCGGCAAATACTGCCCCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGGGGGAGAGGGCCggg                                                                                                                                          <  2:5834620/140‑1 (MQ=255)
   cGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCggg                                                                                                                                          >  1:9045702/1‑140 (MQ=255)
   cGTGAGGTGCAAAAAGAGATGAAAGGGCGTAATCCCAAACATGTCTGGCCGGACGACCCGGCAATTACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCggg                                                                                                                                          <  2:7372378/140‑1 (MQ=255)
      gAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTg                                                                                                                                       >  1:7142389/1‑140 (MQ=255)
       aGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGa                                                                                                                                      >  1:1532663/1‑140 (MQ=255)
        ggTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAg                                                                                                                                     >  1:8126003/1‑140 (MQ=255)
          tGCAAAACTCGATGAACGGGGGTAATCCCAAACTGGTCGGGCCGGACGACCCGGAAAATACTGCACCGACGCGACTGATTAAAATTTTTTCTTA‑TGGTTGTTTTT‑CTCCATCTCCCTGTGGTGGTGGGCGGGGGTGTggg                                                                                                                                   <  2:1672315/140‑1 (MQ=255)
          tGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAggg                                                                                                                                   >  1:8277709/1‑140 (MQ=255)
          tGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAggg                                                                                                                                   >  2:3783727/1‑140 (MQ=255)
            cAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGcc                                                                                                                                 >  1:117377/1‑139 (MQ=255)
               aaaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCa                                                                                                                              >  1:9979351/1‑140 (MQ=255)
               aaaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACCAAAAAGTATTCGTA‑AGGTAGGTTTT‑CTCCCTCTCACTGTGGGAGAGTGCAGGGGTGAGGCCATCa                                                                                                                              >  2:2021313/1‑140 (MQ=255)
                    tattaatgggCTTTAGCCAAAACTTGTCTGGCCGGACGACCCGGAAAATAGTGCACCGACGCGAGGGAGTAAAATTTTTTCTTA‑TGGTTGTTTTT‑CTCCCTCGCCCTGTGGGAGTGGGCGGGGGTGTGGGCATCAgcgcg                                                                                                                         <  2:1433348/133‑1 (MQ=255)
                    taTGAATGGGCGTTATCCCAAACTTGTCGGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAATGTTTTCGTA‑AGGTTGGTTTT‑CTCCCTCGCCCTGTGGGAGAGGGCCGGGGTGTGGGCATCAgcgcg                                                                                                                         <  2:5273745/139‑1 (MQ=38)
                    gctGAAAGGGCGTTACCCCAAACTGGTCTGGCCGGACGCCCCGGCAATTACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAgcgcg                                                                                                                         <  2:7041868/138‑1 (MQ=38)
                    gaTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGcc                                                                                                                                             <  2:3280010/120‑1 (MQ=255)
                    gaTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAgcgcg                                                                                                                         >  1:7012717/1‑140 (MQ=255)
                    gaTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAgcgcg                                                                                                                         >  1:9003015/1‑140 (MQ=255)
                    gaTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAgcgcg                                                                                                                         >  2:3471878/1‑140 (MQ=255)
                    gaTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAgcgcg                                                                                                                         >  2:4864465/1‑140 (MQ=255)
                    gaTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAgcgcg                                                                                                                         >  2:6965939/1‑140 (MQ=255)
                    gaTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGAAGAAACAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCACTGTGGGAGAGGGCAGGGGTGAGGGCATCAgcgag                                                                                                                         >  2:7877378/1‑138 (MQ=39)
                          aGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGAAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGtt                                                                                                                   <  2:10246040/140‑1 (MQ=255)
                           gggCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACgggc                                                                                                                  >  2:323609/1‑137 (MQ=255)
                             gCGTTATCCCAAACTTGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGACAAAGTATTCGGA‑AGGTTGGTTGT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGTgcac                                                                                                                >  2:9151130/1‑140 (MQ=38)
                              cGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGtgggcc                                                                                                               >  1:3072794/1‑135 (MQ=255)
                                ttATCCCAAATTTGTGGGGCGGGACCACCCGCAAAATAGGCCACCGACGCGAGTTACTAAAATTTTTTTGTA‑TGTTTTTTTTT‑CACCCTCTCCGTGGGGTTGTGGGCGGGGGTGGGGCCCTCAGCGCGCACGTtcaccct                                                                                                             <  2:3884762/140‑1 (MQ=255)
                                ttATCCCAAACATGTCGGGCCGGACGACCCGGCAATAACTGCACCGACGCTACGGACTAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCAACTCCCTGGGGGAGTGGGCCGGGGTGAGGGAATCAGCGCGCACGTtcaccct                                                                                                             <  2:685221/140‑1 (MQ=255)
                                ttATCCCAAACAGGACTGGCCGGACCCCCCGGCAAAATCTGCCCCGACGCGACGGACGAAAAAGTATTCGTA‑CGGTGGGTTTA‑CTCCCGCTCCCTGTGGGAGAGGGCCGGGGTGCGGGCATCAGCGCGCACGTtcaccct                                                                                                             <  2:10577500/140‑1 (MQ=37)
                                ttAACCCAAACATGTCGGGCCGCACCCCCCGGCAACTCCGCCACCGACGCGCCGCACTAAAAATTATTCGAC‑CGGTGGGTTTA‑CCCCCACGCCCTGGGGGCGAGGGCCGGGGTGCGGCCCTCAGCGCGCACGTtcaccct                                                                                                             <  1:2669397/140‑1 (MQ=255)
                                taacccaaaaCATGTCTGGCCGGCCGACCCGCCAAAACCTCCACCTCCGCGCCGTACGAAAAATTATGCTAA‑CGGTTGTTTTT‑CCCCCTCTCCCTGGGGGTGAGGGCCGGGGTGCGGGCCTCAGCGCGCACGTtcaccct                                                                                                             <  1:8982326/133‑1 (MQ=255)
                                  aTCCAAAACTGGTCTGCCCGGACCCCCCGCCAAATACGCCACCGCCCCGCCGCACTAAAACTTTTTCTTA‑CGGTTGGTGTA‑CACCCACACCCTGTGGTTGCGGGCGGGGGTGTGGGCCTCAGCGCGCACGTtcaccctcc                                                                                                           <  2:2027502/140‑2 (MQ=255)
                                     ccAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccctcaccc                                                                                                        <  2:7050689/140‑1 (MQ=255)
                                       aaaCATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGTtcaccctcacccac                                                                                                      >  2:8477254/1‑138 (MQ=255)
                                            tGTCTGGCCGGACGACCCGCCAAATACTGCACCGACGCGACGGACGAAAAAGTATGCGTA‑TGGTTGGTTTT‑CTCCCTCACCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCCCCCTAACCct                                                                                                 <  2:2764663/140‑1 (MQ=38)
                                             gtgtGGCCGTACCCCCCGCAAAATCCGCCACCGACGCGACGGACTAAAATGTTTGCGTT‑TGTTTGGTTTC‑CGCCGCCTCCGGGGGGGTGTGGGCGGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCctc                                                                                                <  1:5640245/137‑1 (MQ=255)
                                             gccTGGCCGGACGACCCGGCAAATACTGCACCGCCGCGACGGACGAACAAGTATTCCTA‑AGGTTGGTTTT‑CTCCCACGCCCGGTGGGATAGGGCCGGGGTGAGTGCATCAGCGCGCACGTTCACCCTCACCCTAACCctc                                                                                                <  2:8832484/138‑1 (MQ=255)
                                             gTCTGGCCGGCCGCCCCGCAAATTCCGGCCCCGCCGCGCCGGAC‑TAAAATTTTTTGTACTGTTTGTTTTT‑CTCCCTCTCCCTGTGGGTGTGGGCGGGGGTGTGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCctc                                                                                                <  1:8526688/140‑1 (MQ=12)
                                             gTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑CGGTTGGTTTT‑CGCCCTCTCCCTGTGGGAGAGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCctc                                                                                                <  2:6512051/140‑1 (MQ=255)
                                             gTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAAcccac                                                                                                >  2:10566836/1‑138 (MQ=255)
                                             gTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAGCAGCGCGCACGTGCACCCACCCCCAAAcccac                                                                                                >  2:52156/1‑138 (MQ=38)
                                             gTCGGGCCGGACGCCCCGGAAAATCCTGCACCCACGCGACGGACCAAAATTTATTCTTA‑CGGTTGTTTTA‑CCCCCACCCCCTGTGGGTGTGGCCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCctc                                                                                                <  1:10831801/140‑1 (MQ=255)
                                              tCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTGGACCCACACCCAAAcccaca                                                                                               >  1:6758974/1‑137 (MQ=38)
                                               cgggCCGGACGCCCCGTAAAATACTCCACCGACGCGAGGGACTAAAAATTATTCGTA‑TGTTTGGTTTT‑CTCCCTCTCCCTGTGGGAGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCtctc                                                                                              <  2:1086186/138‑1 (MQ=25)
                                                tGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTcc                                                                                             <  2:9697791/140‑1 (MQ=255)
                                                  gccggccgACCCGGCAAATCCCCCCCCGACGCGCCGGACGAAAAATTTTTCGAA‑AGGTTGGTTTC‑CACCCTCTCCCTGGGGGTGTGGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCt                                                                                           <  1:6543539/140‑1 (MQ=255)
                                                  gCCGGACGCCCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATGCGTA‑CGGTTGGTTTT‑CACCCTCGCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCt                                                                                           <  1:9856758/140‑1 (MQ=39)
                                                        cgacCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGGTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAggg                                                                                     >  2:10753685/1‑140 (MQ=255)
                                                          ccccgcaaaaTCCTGCCCCGAGCCAACGGACGAAAAAGTATTCTTA‑AGGTTGTTTTC‑CTCCCACTCCCGGTGGGAGTGGGCGGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGgag                                                                                   <  2:6671639/133‑1 (MQ=255)
                                                           cccGGCAAATACTGCACCGACGCGACGTACTAAAAAGTATTAGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGGGGGCGAGGGCCGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTACCCCTCTCCCTCAAGGgaga                                                                                  <  2:5489550/140‑1 (MQ=255)
                                                            ccGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CGCCCTCTCCCTGGGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGagag                                                                                 <  2:7678015/140‑1 (MQ=255)
                                                                 aaaGACTCCACCGACCCCACCGACGAAAAAGTATTCGTA‑AGGGTGGTTTT‑CTCCCACTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCACCGCGCACGTTCCCCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGAc                                                                            <  2:7224924/140‑1 (MQ=37)
                                                                         cACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGTTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAg                                                                    <  2:2453807/140‑1 (MQ=255)
                                                                         cACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAGCCGCGCGCACGTGCACCCGCACCCGAACCCACTCCCGCAAGGGAGAGGGGACCGATagag                                                                    >  2:4432342/1‑140 (MQ=37)
                                                                         cACCGACCCGACGGACTAAAAATTATTCTTA‑TGTTTGTTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAg                                                                    <  1:5933365/140‑1 (MQ=37)
                                                                           ccgccgCGCCGCACTAAAATGTTTTCTTT‑CGGTTTGTTTT‑CCCCCTCTCCCGGTGGGTGGGGCCGGGGGTTCGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGca                                                                  <  1:7699909/140‑1 (MQ=19)
                                                                           ccGACGCGACGTACGAAAATGTATTCGAA‑AGGTTGGTTTT‑CACCCACACCCGGGGGGAGTGGGCCGGGGTGTGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGca                                                                  <  1:10540733/140‑1 (MQ=37)
                                                                           ccGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGca                                                                  <  2:4524138/140‑1 (MQ=255)
                                                                             gccgcgacgTACGAAAAAGTATGCTTA‑AGGTTGGATTT‑CTCCCTAGCCCTGTGCGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGcaca                                                                <  2:8657623/138‑1 (MQ=38)
                                                                              acgcgacgGACGAACAAGTATTCGTA‑AGGTAGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGTCACCCACACCCAAACCCACTCCCACAAGGGATATGGGCCCGATCGAGCACaa                                                               >  2:9246589/1‑140 (MQ=25)
                                                                              acgcgacgGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCCCGGGGACCCTCACCCGAAACCGCGCCCGCGAGGGTGAGGGGTACGATCGAggcccc                                                               >  2:9246761/1‑135 (MQ=21)
                                                                              acgcgacgGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGACCCACAACCAAAACCACTACCACAAGGGGGTGGGGACCGCTAGAGcacca                                                               >  1:9739880/1‑138 (MQ=21)
                                                                              acgcgacgGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCGCACCCAAACCCTCTCCCACAAGGGAGAGGGGACCGATCGAGCACaa                                                               >  2:8065899/1‑140 (MQ=255)
                                                                                   acggacgAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCACTCCCTCAAGGGAGAGGGGACCGATCGAGCCCAAAattt                                                          >  2:6028765/1‑140 (MQ=255)
                                                                                    cggacgAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTGGTCCCACCCCCCAAACCACAACCACCAGGGCGAGGGGGCCGCTCGAGcccacaaattg                                                         >  2:1038269/1‑133 (MQ=16)
                                                                                     ggccgAAATAGTATTCTTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGGGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga                                                        <  2:8412333/137‑1 (MQ=255)
                                                                                      gacaAAAAATTATCCTTA‑TGTTTGTTTAC‑CCCCCTCTCCCTGTGGAATCGGGCGGGGGTGGGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgag                                                       <  1:8351772/140‑1 (MQ=25)
                                                                                        ctaaaaaGTATTCGTA‑TGGTGGGTATT‑CTCCCTCACCCTGTGGGTGTGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgagag                                                     <  1:6519769/138‑1 (MQ=38)
                                                                                        ctaaaaaGTATTCGTA‑AGGTTGTTTTT‑CACCCTCTCCCTGTGGGAGTGGTCCGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGATCACAAATTTTgagag                                                     <  2:531724/138‑1 (MQ=38)
                                                                                        cgAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgagag                                                     <  2:9045701/140‑1 (MQ=255)
                                                                                            aatgTATTCGTA‑TGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGTGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGatat                                                 <  2:6574749/137‑1 (MQ=38)
                                                                                                 aTTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGTGCACCCTCCCCCAATCCCACAACCTCCAGGGCGGGGGGACCGATCGCGCCCACAATTTGTGAGATAacttct                                            >  2:10640626/1‑140 (MQ=18)
                                                                                                   tCGTA‑AGTTTGTTTTT‑CTCCCTCTCCGTGTGGGTGTGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttcttc                                          <  1:9443210/140‑1 (MQ=39)
                                                                                                   tCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttcttc                                          <  2:4636325/140‑1 (MQ=255)
                                                                                                   tCGTA‑AGGTTGGTTTG‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttcttc                                          <  1:9900184/140‑1 (MQ=255)
                                                                                                   tCGTA‑AGGTGGGTTTA‑CTCCCTCTCCCGGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttcttc                                          <  2:2543572/140‑1 (MQ=255)
                                                                                                    tcttagggttgtttTG‑CCCCCTCTCCCTGTGGTTGAGGGCGGGGGTGTGGGCAACAGCGCGCACGTTCACCCTGACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttcttc                                          <  1:8113939/134‑1 (MQ=255)
                                                                                                    tcttacggtcggttTT‑CCCCCACCCCCGTTGGTAGCGGGCCGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttcttc                                          <  1:524287/134‑1 (MQ=37)
                                                                                                    gcgtcgggttggttAC‑CCCCCACCCCCTGGGGGTGCGGCCGGGGGTGCGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttcttc                                          <  1:2969326/134‑1 (MQ=25)
                                                                                                    cGTA‑TGGTTTTTTTT‑CTCCATCACGCTGTGTGTGTGGGCCGGGGTTTGGGCATCAGCGCGCACGTTCACCCTCACCATAACCATCTCCCTCAAGGGAGAGTGGACCGATCGAGCACAAATTTTGAGAGATAtcttcttct                                         <  1:1754670/140‑1 (MQ=255)
                                                                                                         tggtgggttTT‑CACCCTCTCCGTGTGGGTGTGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCt                                     <  1:7759459/139‑1 (MQ=38)
                                                                                                             tggttTTCCCCCCTCTCCCTGGGGGAGTGGGCGGGGGTGGGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGt                                  <  1:4221158/140‑1 (MQ=38)
                                                                                                                    tccccctccccctGTGGGTGTGGGCGGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaa                           <  1:2102230/137‑1 (MQ=39)
                                                                                                                      tccctctccctGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTGCACCCTCACCCAAACCCTCTCCCTCCAGGGAGAGGGGCCCGAGCGAGCACAAATTTTGAGAGATATATTATTATGTCTTGTAACagaaga                         >  2:8187982/1‑140 (MQ=37)
                                                                                                                        cctctcccgGTGGGAGTGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAA‑c                      <  1:10269706/140‑1 (MQ=255)
                                                                                                                                tgtgGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGTTCACCCACACCCTAACCCTCTCCCGCAAGGGAGAGGGGTCCGATCGAGCACAAATTTTGTGAGATATATTATTCTGTGTTGTAAAAGAAGAA‑CAGAAAATc              >  2:9814513/1‑140 (MQ=255)
                                                                                                                                      agagGGCCGGGGTGAGGGCATCAGCGCGCCCGTGCACCCCCACCATAAACCACACCCCCAAGGGAGAGGGGACCTATTCATCTCAAAATGTGTGAGATTTAATCTTTGGTATTGTAACATAAGAA‑CAGACAATCGGGCtg        >  2:7598988/1‑139 (MQ=255)
                                                                                                                                      agagGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGGCCGGTCGAGCACAAATTTTGAGAGATATCTTCTTCTGTGGTGTAACAGTAGAA‑CAGAAAATCGGGcgt        >  2:9921225/1‑138 (MQ=255)
                                                                                                                                       gcgggCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCGCTCCCTCAAGGGAGAGGGGACCGATCGCGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAA‑CAGAAACTCGGGCttt       <  2:5042570/138‑1 (MQ=255)
                                                                                                                                       gagGGCCGGGGTGAGGGCATCAGCGCGCACGTTGACCCTCAACCAAAACCAATACCACAAGGGGGTGGGGACCGCTAGGGCACAAAATTTGTGTGATATATTATTATTTCTTTTTACAGAATAA‑CAGAAAAACGGGCttt       >  2:6071359/1‑140 (MQ=255)
                                                                                                                                       gagGGCCGGGGTGACGGCATCAGCGCGCACGTTCACCCTCACCCTACCCCTCTCCCTCAAGTGAGAGGGGACCGCTCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAA‑CAGAAAATCGGGCttt       <  2:7764911/140‑1 (MQ=255)
                                                                                                                                         gggCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAA‑CAGAAAATCGGGCTTTTg     <  1:3778060/140‑1 (MQ=255)
                                                                                                                                          ggCCGGGGTGAGGGCATCAGCGCGCACGTTGAACCCCCCCCAAACCCACAACCACCAGGGCGTGGGGACCCATATGGCACAAAATTTGTGAGATATATTATTAGTTCTTTTTAAAGAAGAACCCAAAAAACGGGGTTTTg     >  2:9345992/1‑140 (MQ=255)
                                                                                                                                            ccGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAA‑CAGAAAATCGGGCTTTTgcgc  <  1:10753686/140‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                           
TACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAA‑CAGAAAATCGGGCTTTTGCGC  >  NZ_CP025268/164261‑164538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑