Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP025268164,4120AC30.7% 127.5 / 32.0 140intergenic (+47/‑149)hrpB/mrcBATP‑dependent helicase HrpB/bifunctional glycosyl transferase/transpeptidase
Reads supporting (aligned to +/- strand):  ref base A (36/61);  new base C (7/36);  total (43/97)
Fisher's exact test for biased strand distribution p-value = 1.68e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 141 total aligned reads displayed.

CAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCT  >  NZ_CP025268/164273‑164540
                                                                                                                                             |                                                                                                                                
   aaaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCa                                                                                                                               >  1:9979351/1‑140 (MQ=255)
       atattaaaGGGCGTTAACACAAATATGTCTGGCCGGACGACCCGGAAAATACTGCACCGACGCGACGTACTAAAATGTATTCGTA‑TGGTTGTTTTA‑CACCCTCGCCTTGTGGGTTTGGGCCGGGGTGTGGGCCTCAgcgc                                                                                                                           <  2:5004627/135‑1 (MQ=255)
        tattaatgggCTTTAGCCAAAACTTGTCTGGCCGGACGACCCGGAAAATAGTGCACCGACGCGAGGGAGTAAAATTTTTTCTTA‑TGGTTGTTTTT‑CTCCCTCGCCCTGTGGGAGTGGGCGGGGGTGTGGGCATCAgcgcg                                                                                                                          <  2:1433348/133‑1 (MQ=255)
        taTGAATGGGGGTTATCCCAAACTTGTGTGGCCGGACGACCCGGCAATTACTGCACCGAGGCGACGGACTAAAATGTTTTCGTA‑TGGTTGGTTTT‑CTCCCTCGCCCTGTGGGAGTGGGCCGGGGTGTGGGCATCAgcgcg                                                                                                                          <  2:1246256/139‑1 (MQ=255)
        taTGAATGGGCGTTATCCCAAACTTGTCGGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAATGTTTTCGTA‑AGGTTGGTTTT‑CTCCCTCGCCCTGTGGGAGAGGGCCGGGGTGTGGGCATCAgcgcg                                                                                                                          <  2:5273745/139‑1 (MQ=38)
        taTGAAAGGGCGTTAGCCAAAACTTGTCTGTCCGGACGACCCGGCAAATACGGCACCGACGCTACGGACGAACATTTTTTCGTA‑TTGTTGTTTTT‑CTCCCTATCCCTTGGGGAGAGGGCCGGGTTGTGGGCCTCAgcgcg                                                                                                                          <  2:1879192/139‑1 (MQ=255)
        gctGAAAGGGCGTTACCCCAAACTGGTCTGGCCGGACGCCCCGGCAATTACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAgcgcg                                                                                                                          <  2:7041868/138‑1 (MQ=38)
        gaTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAgcgcg                                                                                                                          >  1:7012717/1‑140 (MQ=255)
        gaTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAgcgcg                                                                                                                          >  2:6965939/1‑140 (MQ=255)
        gaTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAgcgcg                                                                                                                          >  2:3471878/1‑140 (MQ=255)
        gaTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAgcgcg                                                                                                                          >  2:5591362/1‑140 (MQ=255)
        gaTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAGCAgcgcg                                                                                                                          >  1:9382140/1‑140 (MQ=255)
        gaTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGAAGAAACAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCACTGTGGGAGAGGGCAGGGGTGAGGGCATCAgcgag                                                                                                                          >  2:7877378/1‑138 (MQ=39)
             aaGGGCGTTATCCAAAACATGTCTGGCCGGACGACCCGGCAATTACTGCCCCGCCGCGCCGGACGAAAAAGTTTTCTTA‑CGTTTGGTTTT‑CTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGt                                                                                                                     <  2:483798/140‑1 (MQ=25)
              tggggGTTATCCCAAACTTGTCTGGCCGGACGACCCGGCAATTCCTGCCCCGACGCGAGGGACGAAAAAGTATTCGTA‑CGGTGGGTTTT‑CTCCCTCTCCCGGGGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGtt                                                                                                                    <  2:7359941/139‑1 (MQ=37)
              tgggCGTTATCCCAAACTTGTCTGGCCGGACGACCCGGCAAATAGTGCACCGACGGGACGGACGAAAATGTTTTCGTA‑TGTTTGGTTTT‑CTCCCTCTCCCTGTGGGTGAGGGCCGGGGTGAGGGCATCAGCGCGCACGtt                                                                                                                    <  2:8106812/139‑1 (MQ=37)
              aggggtttATCCCAACCCTGTCGGGCCGGACGACCCGGAAAACACTCCACCGACGCGACGGACTAAAAAGTTTTCGTA‑CGGTTGGTTTT‑CGCCCGCTCCCTGGGGGAGAGGGCCGGGGGGTGGGCATCAGCGCGCACGtt                                                                                                                    <  2:9302604/134‑1 (MQ=255)
              aGGGCGTTATCCCAAACTTGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGTACTAAAAATTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGTTAGGGCCGGGGTGAGGGCATCAGCGCGCACGtt                                                                                                                    <  2:7190983/140‑1 (MQ=38)
               gggCGTTATCCCAACCATGTCTGGCGGGACGACCGGGCAAATACTGCACCGACGGGTGGTACGAAAATGTATTGGTA‑AGGTTGTTTTT‑GTCCCTCTCCGTGTGGGTGTGGGCGGGGGTGCGGGCATCAGCGCGCACGTtc                                                                                                                   <  2:7573889/140‑1 (MQ=255)
               gggCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACgggc                                                                                                                   >  2:323609/1‑137 (MQ=255)
                 gCGTTATCCCAAACTTGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGACAAAGTATTCGGA‑AGGTTGGTTGT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGTgcac                                                                                                                 >  2:9151130/1‑140 (MQ=38)
                  cGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGtgggcc                                                                                                                >  1:3072794/1‑135 (MQ=255)
                   gTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTtcaccc                                                                                                               <  2:10586433/140‑1 (MQ=255)
                   gTTACCCAAACCTTGTGTGGCCGGACGACCCGGAAAATACTGCACCGACGCGCCGGACTAAAATTAATGCGTA‑TGGTTGGTTTT‑CCCCCACCCCCTGGGGGAGCGGGCGGGGGTGAGGGCCTCAGCGCGCACGTtcaccc                                                                                                               <  1:10781351/140‑1 (MQ=255)
                    ttATCCCAACCATGACTGGCCGCACGACCCCGCAACTCCGGCCCCGCCGCGACGGCAGAAAAAGTATTCTTA‑AGGTTGGTTTT‑CTCCCTCGCCCTGTGGGAGAGGGCCGGGGTGCGGGCATCAGCGCGCACGGtcaccct                                                                                                              <  2:7613016/140‑1 (MQ=25)
                    ttATCCCAAATTTGTGGGGCGGGACCACCCGCAAAATAGGCCACCGACGCGAGTTACTAAAATTTTTTTGTA‑TGTTTTTTTTT‑CACCCTCTCCGTGGGGTTGTGGGCGGGGGTGGGGCCCTCAGCGCGCACGTtcaccct                                                                                                              <  2:3884762/140‑1 (MQ=255)
                    ttATCCCAAACATGTCGGGCCGGACGACCCGGCAATAACTGCACCGACGCTACGGACTAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCAACTCCCTGGGGGAGTGGGCCGGGGTGAGGGAATCAGCGCGCACGTtcaccct                                                                                                              <  2:685221/140‑1 (MQ=255)
                    ttATCCCAAACAGGACTGGCCGGACCCCCCGGCAAAATCTGCCCCGACGCGACGGACGAAAAAGTATTCGTA‑CGGTGGGTTTA‑CTCCCGCTCCCTGTGGGAGAGGGCCGGGGTGCGGGCATCAGCGCGCACGTtcaccct                                                                                                              <  2:10577500/140‑1 (MQ=37)
                    ttAACCCAAACTTTTCGGGCGGGCCGACCGGGAAATTAATGCACCGACGCGTCGTACTAATATTTTTTCTTA‑TGGTTGGTTTT‑CTCCCTCCCCGTGGGGTAGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTtcaccct                                                                                                              <  2:2518157/140‑1 (MQ=255)
                    ttAACCCAAACATGTCGGGCCGCACCCCCCGGCAACTCCGCCACCGACGCGCCGCACTAAAAATTATTCGAC‑CGGTGGGTTTA‑CCCCCACGCCCTGGGGGCGAGGGCCGGGGTGCGGCCCTCAGCGCGCACGTtcaccct                                                                                                              <  1:2669397/140‑1 (MQ=255)
                    ttAACCAAAACTTGTCGGGCCGGACGCCCCGGAAATTACTGCACCGACGCGAGGGACTAAAATTTATTGGTA‑TGTTGGGTTTT‑CTCCCTCTCCCGGGGGGTGTGGGCGGGGGTGAGGGCCTCAGCGCGCACGTtcaccct                                                                                                              <  1:7183536/140‑1 (MQ=255)
                    taacccaaaaCATGTCTGGCCGGCCGACCCGCCAAAACCTCCACCTCCGCGCCGTACGAAAAATTATGCTAA‑CGGTTGTTTTT‑CCCCCTCTCCCTGGGGGTGAGGGCCGGGGTGCGGGCCTCAGCGCGCACGTtcaccct                                                                                                              <  1:8982326/133‑1 (MQ=255)
                      aTCCCAAACATGTCTGGCCGGACGACCCGGCAACTACTGCACCGACGCGACGGACGAAAAAGTATGCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCGCAGCGCGCACGTtcacccgca                                                                                                            >  2:10312056/1‑140 (MQ=38)
                      aTCCAAAACTGGTCTGCCCGGACCCCCCGCCAAATACGCCACCGCCCCGCCGCACTAAAACTTTTTCTTA‑CGGTTGGTGTA‑CACCCACACCCTGTGGTTGCGGGCGGGGGTGTGGGCCTCAGCGCGCACGTtcaccctcc                                                                                                            <  2:2027502/140‑2 (MQ=255)
                         ccAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccctcaccc                                                                                                         <  2:7050689/140‑1 (MQ=255)
                           aaaCATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGTtcaccctcacccac                                                                                                       >  2:8477254/1‑138 (MQ=255)
                               aggTCGGGCCGTACGCCCCGCCAAATACTCCACCTACGCGCCGGACGAAAAATTTTTCTTA‑TGGTTGGTTTT‑CTCCCACTCCATGTGGCTGTGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAAccc                                                                                                   <  2:1083908/138‑1 (MQ=255)
                                tGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCct                                                                                                  <  2:10415579/140‑1 (MQ=255)
                                tGTCTGGCCGGACGACCCGCCAAATACTGCACCGACGCGACGGACGAAAAAGTATGCGTA‑TGGTTGGTTTT‑CTCCCTCACCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCCCCCTAACCct                                                                                                  <  2:2764663/140‑1 (MQ=38)
                                gggcggcccGCACGCCCGGCCAAATACGCCACCGACGCGAGGGACGAAAATGAATTCTTA‑CGGTGGGTTTC‑CACCCTCCCCCTGTGGGAGAGGCCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCct                                                                                                  <  2:5174121/133‑1 (MQ=255)
                                 gccTGGCCGGACGACCCGGCAAATACTGCACCGCCGCGACGGACGAACAAGTATTCCTA‑AGGTTGGTTTT‑CTCCCACGCCCGGTGGGATAGGGCCGGGGTGAGTGCATCAGCGCGCACGTTCACCCTCACCCTAACCctc                                                                                                 <  2:8832484/138‑1 (MQ=255)
                                 gTCTGGCCGGCCGCCCCGCAAATTCCGGCCCCGCCGCGCCGGAC‑TAAAATTTTTTGTACTGTTTGTTTTT‑CTCCCTCTCCCTGTGGGTGTGGGCGGGGGTGTGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCctc                                                                                                 <  1:8526688/140‑1 (MQ=12)
                                 gTCTGGCCGGACGACCCGGCACATACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCCGCGCGCACGTGCACCCTCACCCAAAcccgc                                                                                                 >  2:7952400/1‑138 (MQ=38)
                                 gTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGGCGCCCTCACCCGAAcccac                                                                                                 >  1:6265207/1‑138 (MQ=38)
                                 gTCTGGCCGGACGACCCGGAAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCATGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCctc                                                                                                 <  2:5949432/140‑1 (MQ=255)
                                 gTCGGGCCGGACGCCCCGGAAAATCCTGCACCCACGCGACGGACCAAAATTTATTCTTA‑CGGTTGTTTTA‑CCCCCACCCCCTGTGGGTGTGGCCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCctc                                                                                                 <  1:10831801/140‑1 (MQ=255)
                                  tCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTGGACCCACACCCAAAcccaca                                                                                                >  1:6758974/1‑137 (MQ=38)
                                   cgggCCGGACGCCCCGGCACAGACTGCACCGACGCGACGGACGAACAAGTATTCGTA‑AGGTTGGTGTT‑CTCCCTCTCCCTGGGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCtctc                                                                                               <  2:6128643/138‑1 (MQ=38)
                                    tGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTcc                                                                                              <  2:9697791/140‑1 (MQ=255)
                                      gccggccgCCCCGCCAAACACCGCACCGACGCGCCGGCCTAAAATGTATTCGTA‑AGGTTGGTTTT‑CGCCCTCCCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCt                                                                                            <  1:9867906/140‑1 (MQ=25)
                                      gCCGGACGCCCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATGCGTA‑CGGTTGGTTTT‑CACCCTCGCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCt                                                                                            <  1:9856758/140‑1 (MQ=39)
                                      gCCGGACGACCCGGCAAATACGGCACCGACGCGCCGGACGAAAAAGTATTGGGA‑CGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCt                                                                                            <  1:2752859/140‑1 (MQ=38)
                                          gacgacCCGGCAAATACGGCACCGCCGCGCCGGACGAAAAAGTTTTCGTA‑AGGTTGGTGTT‑CGCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAg                                                                                        <  2:9606001/140‑1 (MQ=38)
                                            cgacCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGGTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAggg                                                                                      >  2:10753685/1‑140 (MQ=255)
                                             gacCCGGCAAATACGGCACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGga                                                                                     <  2:9905020/140‑1 (MQ=255)
                                              ccccgcaaaaTCCTGCCCCGAGCCAACGGACGAAAAAGTATTCTTA‑AGGTTGTTTTC‑CTCCCACTCCCGGTGGGAGTGGGCGGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGgag                                                                                    <  2:6671639/133‑1 (MQ=255)
                                              ccccGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑TGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCGGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGgag                                                                                    <  1:8156908/139‑1 (MQ=255)
                                               cccGGCAAATACTGCACCGACGCGACGTACTAAAAAGTATTAGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGGGGGCGAGGGCCGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTACCCCTCTCCCTCAAGGgaga                                                                                   <  2:5489550/140‑1 (MQ=255)
                                                ccGGCAAATACTGCACCGACGCGACGGACGAAAAAGTTTTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGagag                                                                                  <  2:7782208/140‑1 (MQ=255)
                                                ccGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CGCCCTCTCCCTGGGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGagag                                                                                  <  2:7678015/140‑1 (MQ=255)
                                                  ggaaaaTACTCCACCGACGCGAGGTACCAAAATGTATTCGTA‑GGGTTGGTTTA‑CCCCCTCTCCCTGTGGGTGCGGGCGGGGGTGGGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAggg                                                                                <  1:901343/137‑1 (MQ=255)
                                                             cACCGACGCGACGGACGAAAAATTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGTAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAg                                                                     <  2:5288245/140‑1 (MQ=255)
                                                             cACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAGCCGCGCGCACGTGCACCCGCACCCGAACCCACTCCCGCAAGGGAGAGGGGACCGATagag                                                                     >  2:4432342/1‑140 (MQ=37)
                                                             cACCGACCCGACGGACTAAAAATTATTCTTA‑TGTTTGTTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAg                                                                     <  1:5933365/140‑1 (MQ=37)
                                                               ccGACGCTACGGACTAAATTGTTTTCTTA‑TGTTTGGTTTT‑CGCCCTCTCCCTGTGGGATTGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGca                                                                   <  1:8857671/140‑1 (MQ=37)
                                                               ccGACGCGACGTACGAAAATGTATTCGAA‑AGGTTGGTTTT‑CACCCACACCCGGGGGGAGTGGGCCGGGGTGTGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGca                                                                   <  1:10540733/140‑1 (MQ=37)
                                                               ccGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGca                                                                   <  2:4524138/140‑1 (MQ=255)
                                                                 gccgcgacgTACGAAAAAGTATGCTTA‑AGGTTGGATTT‑CTCCCTAGCCCTGTGCGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGcaca                                                                 <  2:8657623/138‑1 (MQ=38)
                                                                  acgcgacgGACGAACAAGTATTCGTA‑AGGTAGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGTCACCCACACCCAAACCCACTCCCACAAGGGATATGGGCCCGATCGAGCACaa                                                                >  2:9246589/1‑140 (MQ=25)
                                                                  acgcgacgGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCCCGGGGACCCTCACCCGAAACCGCGCCCGCGAGGGTGAGGGGTACGATCGAggcccc                                                                >  2:9246761/1‑135 (MQ=21)
                                                                  acgcgacgGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGACCCACAACCAAAACCACTACCACAAGGGGGTGGGGACCGCTAGAGcacca                                                                >  1:9739880/1‑138 (MQ=21)
                                                                        cggacgAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTGGTCCCACCCCCCAAACCACAACCACCAGGGCGAGGGGGCCGCTCGAGcccacaaattg                                                          >  2:1038269/1‑133 (MQ=16)
                                                                        cggacgAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGTTCACCCTCACCCTAACCCACTCCCTCAACGGAGAGGGGACCGATCGAGCACAAAttttt                                                          >  2:6657592/1‑139 (MQ=255)
                                                                          gactAAAATGTATTCGTA‑TGGTTTGTTTT‑CGCACTCTCCCTGGGGGCGTGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgag                                                        <  1:8351643/140‑1 (MQ=37)
                                                                            ctaaaaaGTATTCGTA‑TGGTGGGTATT‑CTCCCTCACCCTGTGGGTGTGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgagag                                                      <  1:6519769/138‑1 (MQ=38)
                                                                            cgAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgagag                                                      <  2:9045701/140‑1 (MQ=255)
                                                                              aaaaaGTTTTCTTA‑TGTTTGTTTTT‑CACCCACGCCCTGTGGTATAGGGCCGGGGTGAGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGat                                                    <  1:795066/140‑1 (MQ=37)
                                                                              aaaaaGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGat                                                    <  2:3684892/140‑1 (MQ=255)
                                                                                aatgTATTCGTA‑TGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGTGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGatat                                                  <  2:6574749/137‑1 (MQ=38)
                                                                                       tCGTA‑TGGTTGTTTTA‑CCCCCTCTCCCTGTGGTCGTGGGCCGGGGTGAGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttcttc                                           <  1:7721434/140‑1 (MQ=38)
                                                                                       tCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttcttc                                           <  1:9922719/140‑1 (MQ=255)
                                                                                       tCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttcttc                                           <  2:4636325/140‑1 (MQ=255)
                                                                                       tCGTA‑AGGTGGGTTTA‑CTCCCTCTCCCGGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttcttc                                           <  2:2543572/140‑1 (MQ=255)
                                                                                        tcttacggtcggttTT‑CCCCCACCCCCGTTGGTAGCGGGCCGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttcttc                                           <  1:524287/134‑1 (MQ=37)
                                                                                        cGTA‑TGGTTTTTTTT‑CTCCATCACGCTGTGTGTGTGGGCCGGGGTTTGGGCATCAGCGCGCACGTTCACCCTCACCATAACCATCTCCCTCAAGGGAGAGTGGACCGATCGAGCACAAATTTTGAGAGATAtcttcttct                                          <  1:1754670/140‑1 (MQ=255)
                                                                                          tA‑AGGTTGGTTTT‑CTCCCTCACCCTGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGt                                        <  1:4778446/140‑1 (MQ=255)
                                                                                             tggtgggttTT‑CACCCTCTCCGTGTGGGTGTGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCt                                      <  1:7759459/139‑1 (MQ=38)
                                                                                                 tggttTTCCCCCCTCTCCCTGGGGGAGTGGGCGGGGGTGGGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGt                                   <  1:4221158/140‑1 (MQ=38)
                                                                                                        tccccctccccctGTGGGTGTGGGCGGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaa                            <  1:2102230/137‑1 (MQ=39)
                                                                                                          tccctctccctGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTGCACCCTCACCCAAACCCTCTCCCTCCAGGGAGAGGGGCCCGAGCGAGCACAAATTTTGAGAGATATATTATTATGTCTTGTAACagaaga                          >  2:8187982/1‑140 (MQ=37)
                                                                                                            cctctcccgGTGGGAGTGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAAc                        <  1:10269706/140‑1 (MQ=255)
                                                                                                                          agagGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGGCCGGTCGAGCACAAATTTTGAGAGATATCTTCTTCTGTGGTGTAACAGTAGAACAGAAAATCGGGcgt          >  2:9921225/1‑138 (MQ=255)
                                                                                                                           gcgggCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCGCTCCCTCAAGGGAGAGGGGACCGATCGCGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAACTCGGGCttt         <  2:5042570/138‑1 (MQ=255)
                                                                                                                           gagGGCCGGGGTGAGGGCATCAGCGCGCACGTTGACCCTCAACCAAAACCAATACCACAAGGGGGTGGGGACCGCTAGGGCACAAAATTTGTGTGATATATTATTATTTCTTTTTACAGAATAACAGAAAAACGGGCttt         >  2:6071359/1‑140 (MQ=255)
                                                                                                                             gggCGGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTg       <  1:6657593/140‑1 (MQ=255)
                                                                                                                             gggCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTg       <  1:1626299/140‑1 (MQ=255)
                                                                                                                             gggCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTg       <  1:3778060/140‑1 (MQ=255)
                                                                                                                             ggccgggggTGCGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTg       <  1:1038269/135‑1 (MQ=39)
                                                                                                                              ggCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCACAACCAAAACCACACCCCCAAGGGAGAGGGGGCCCCTCCAGCACAAAATTTTAGAGATAAATTATTATGTAATTTAACAAAATAAAAAAAAAAAGGGCgttttg      >  2:234933/1‑138 (MQ=255)
                                                                                                                                  ggggTTAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAACTCGGGCTTTTGCGCCt  <  2:3244482/140‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                
CAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTA‑AGGTTGGTTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCT  >  NZ_CP025268/164273‑164540

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑