Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,219,333	G→A	intergenic (‑230/+27)	iraM ← / ← CXP41_RS24360	anti‑adapter protein IraM/protein YmgK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,219,333	0	G	A	100.0%	24.8 / NA	8	intergenic (‑230/+27)	iraM/CXP41_RS24360	anti‑adapter protein IraM/protein YmgK
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/4);  total (4/4)

AATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATGCATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTT  >  NZ_CP025268/1219252‑1219417                                                                                  |                                                                                     aaTTGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCa                            <  1:2940967/140‑1 (MQ=255)     gATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAAttt                        >  1:3220393/1‑140 (MQ=255)                                                                          aaTGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCa                            >  2:5936826/1‑67 (MQ=255)                                                                          aaTGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATCCTACTTTGTGCCa                            <  1:5936826/67‑1 (MQ=255)                                                                           aTGTTATACATTCAAATGACTGAGCATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCttt  >  1:8239810/1‑92 (MQ=255)                                                                           aTGTTATACATTCAAATGACTGAGCATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCttt  <  2:8239809/92‑1 (MQ=255)                                                                           aTGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACAt                                                   >  1:4692460/1‑43 (MQ=255)                                                                           aTGTTATACATTCAAATGACTGAACATCCTGTAATTAAAACAt                                                   <  2:4692460/43‑1 (MQ=255)                                                                                  |                                                                                     AATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGTTTAGCTATAATGTTATGCATTCAAATGACTGAACATCCTGTAATTAAAACATAGCCTTTATGCTACTTTGTGCCAATTTGCTAAACATTATGGTTGCCTTT  >  NZ_CP025268/1219252‑1219417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑