BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,236,662	T→C	A277A (GCA→GCG)	hlyE ←	hemolysin HlyE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,236,662	0	T	C	99.9%	8048.8 / NA	2298	A277A (GCA→GCG)	hlyE	hemolysin HlyE
Reads supporting (aligned to +/- strand): ref base T (0/1); major base C (1467/825); minor base A (3/0); total (1472/826)
Fisher's exact test for biased strand distribution p-value = 5.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2300 total aligned reads displayed.

TGTTTTTAAATGTGATAAACCTTATACCTTGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCTGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCG  >  NZ_CP025268/1236525‑1236798

     ttAAATGTGATAAACCTTATACCTTGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGcttctt                                                                                                                                   >  2:5204581/1‑140 (MQ=255)

     ttAAATGTGATAAACCTTATACCTTGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGcttctt                                                                                                                                   >  2:3218168/1‑140 (MQ=255)

     ttAAATGTGATAAACCTTATACCTTGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGcttctt                                                                                                                                   >  2:7542676/1‑140 (MQ=255)

               tAAACCTTATACCTTGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCaaaga                                                                                                                         <  1:6513172/140‑1 (MQ=255)

                      tataCCTTGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCAtt                                                                                                                  >  2:2241377/1‑140 (MQ=255)

                      tataCCTTGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCAtt                                                                                                                  >  1:9849220/1‑140 (MQ=255)

                      tataCCTTGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCAtt                                                                                                                  >  1:897294/1‑140 (MQ=255)

                       ataCCTTGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTa                                                                                                                 >  1:10018423/1‑140 (MQ=255)

                        taCCTTGAGTACATGGAGAGAAAAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTaa                                                                                                                >  1:5986256/1‑140 (MQ=255)

                         aCCTTGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTaaa                                                                                                               <  2:10814449/140‑1 (MQ=255)

                         aCCTTGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTaaa                                                                                                               <  1:5484913/140‑1 (MQ=255)

                         aCCTTGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTaaa                                                                                                               <  2:8291571/140‑1 (MQ=255)

                             tGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCAt                                                                                                                   >  2:338278/1‑132 (MQ=255)

                             tGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTaaa                                                                                                               <  2:599744/136‑1 (MQ=255)

                             tGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTaaa                                                                                                               <  1:2798842/136‑1 (MQ=255)

                             tGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAatcat                                                                                                           >  2:8682648/1‑140 (MQ=255)

                             tGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAatcat                                                                                                           >  2:5461331/1‑140 (MQ=255)

                             tGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAatcat                                                                                                           >  1:7427034/1‑140 (MQ=255)

                             tGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAatcat                                                                                                           >  1:7789829/1‑140 (MQ=255)

                             tGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAatcat                                                                                                           >  2:4829916/1‑140 (MQ=255)

                             tGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAatcat                                                                                                           >  1:2604285/1‑140 (MQ=255)

                             tGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAatcat                                                                                                           >  1:3413470/1‑140 (MQ=255)

                              gAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAatcatc                                                                                                          >  1:8551832/1‑140 (MQ=255)

                              gAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAatcatc                                                                                                          >  1:2397512/1‑140 (MQ=255)

                                   cATGGAGAGTATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAAt                                                                                                     <  1:6310110/140‑1 (MQ=255)

                                   cATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAAt                                                                                                     <  2:1360563/140‑1 (MQ=255)

                                    aTGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATc                                                                                                    <  1:4513972/140‑1 (MQ=255)

                                    aTGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATc                                                                                                    >  2:8897576/1‑140 (MQ=255)

                                    aTGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATc                                                                                                    <  2:1190179/140‑1 (MQ=255)

                                    aTGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATc                                                                                                    >  1:3015869/1‑140 (MQ=255)

                                     tGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCa                                                                                                   >  1:10286722/1‑140 (MQ=255)

                                     tGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCa                                                                                                   >  1:5125550/1‑140 (MQ=255)

                                      ggAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAGGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCaa                                                                                                  >  2:6975082/1‑140 (MQ=255)

                                      ggAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCaa                                                                                                  >  2:3038333/1‑140 (MQ=255)

                                      ggAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCaa                                                                                                  >  2:4961097/1‑140 (MQ=255)

                                      ggAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCa                                                                                                   <  1:578822/139‑1 (MQ=255)

                                         gagaATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGt                                                                                               >  2:7696318/1‑140 (MQ=255)

                                         gagaATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGt                                                                                               >  2:2852636/1‑140 (MQ=255)

                                         gagaATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGt                                                                                               >  2:9368529/1‑140 (MQ=255)

                                         gagaATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGt                                                                                               >  2:1823148/1‑140 (MQ=255)

                                           gaATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTaaa                                                                                                               <  1:7578536/122‑1 (MQ=255)

                                           gaATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAg                                                                                             >  2:2638026/1‑140 (MQ=255)

                                           gaATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGAATTAAATCATCATAATCAACGTAg                                                                                             >  2:9190785/1‑140 (MQ=255)

                                              taatCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAAt                                                                                          >  1:10834029/1‑140 (MQ=255)

                                                 tCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTg                                                                                       >  2:735683/1‑140 (MQ=255)

                                                  cGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTgg                                                                                      >  1:6909946/1‑140 (MQ=255)

                                                    cTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAAt                                                                                                     >  1:3209146/1‑123 (MQ=255)

                                                    cTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGgtt                                                                                    >  2:6178607/1‑140 (MQ=255)

                                                      tATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGgttgt                                                                                  >  2:6782708/1‑140 (MQ=255)

                                                      tATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGgttgt                                                                                  >  1:5317598/1‑140 (MQ=255)

                                                      tATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGgttgt                                                                                  >  1:7051866/1‑140 (MQ=255)

                                                        tCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGttt                                                                                <  1:3691033/140‑1 (MQ=255)

                                                        tCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGttt                                                                                <  1:833921/140‑1 (MQ=255)

                                                            aCTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGt                                                                            >  1:9591134/1‑140 (MQ=255)

                                                            aCTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGt                                                                            >  2:9033236/1‑140 (MQ=255)

                                                             cTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGtt                                                                           >  2:7890099/1‑140 (MQ=255)

                                                              ttCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGttt                                                                          >  2:6557682/1‑140 (MQ=255)

                                                               tCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACg                                                                                                >  2:5680598/1‑117 (MQ=255)

                                                                 aGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCg                                                                       >  2:10054939/1‑140 (MQ=255)

                                                                   gTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTa                                                                                              <  2:6345692/115‑1 (MQ=255)

                                                                      ccTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACg                                                                                                <  1:6716346/110‑1 (MQ=255)

                                                                      ccTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGttttt                                                                  >  2:2434436/1‑140 (MQ=255)

                                                                      ccTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGttttt                                                                  >  2:10264103/1‑140 (MQ=255)

                                                                            agagTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCaaagaa                                                                                                                        <  1:8013456/80‑1 (MQ=255)

                                                                                 gtCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGAt                                                       >  2:7139589/1‑140 (MQ=255)

                                                                                  tCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATg                                                      >  2:9548085/1‑140 (MQ=255)

                                                                                  tCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATg                                                      >  1:2089656/1‑140 (MQ=255)

                                                                                    tttttACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTaaa                                                                                                               >  2:5861108/1‑82 (MQ=255)

                                                                                    tttttACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATggc                                                    <  1:1278158/140‑1 (MQ=255)

                                                                                            gtgtCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTAttt                                            >  1:3902280/1‑140 (MQ=255)

                                                                                            gtgtCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTAttt                                            >  2:467044/1‑140 (MQ=255)

                                                                                                       gATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAAt                                 >  2:4301528/1‑140 (MQ=255)

                                                                                                         tACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAAttt                               >  1:4697555/1‑140 (MQ=255)

                                                                                                           cTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCa                             >  1:9123454/1‑140 (MQ=255)

                                                                                                           cTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCa                             >  2:10615088/1‑140 (MQ=255)

                                                                                                              aTTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACg                                                                                                <  1:10498929/70‑1 (MQ=255)

                                                                                                              aTTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAAtt                          >  2:3599100/1‑140 (MQ=255)

                                                                                                                tACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTg                        >  1:1474316/1‑140 (MQ=255)

                                                                                                                tACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTg                        >  1:7252960/1‑140 (MQ=255)

                                                                                                                tACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTg                        >  1:2246267/1‑140 (MQ=255)

                                                                                                                 aCAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTgg                       <  2:849648/140‑1 (MQ=255)

                                                                                                                   aGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTggcg                     <  1:8730162/140‑1 (MQ=255)

                                                                                                                     gtgtTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTggcggc                   >  1:6124357/1‑140 (MQ=255)

                                                                                                                     gtgtTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTggcggc                   >  2:9755557/1‑140 (MQ=255)

                                                                                                                        ttAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACg                                                                                                <  1:4113136/60‑1 (MQ=255)

                                                                                                                        ttAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTggcg                     >  2:3807280/1‑135 (MQ=255)

                                                                                                                        ttAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATc                >  2:4035116/1‑140 (MQ=255)

                                                                                                                        ttAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATc                >  1:9355614/1‑140 (MQ=255)

                                                                                                                        ttAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATc                >  2:1624371/1‑140 (MQ=255)

                                                                                                                         tAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAGTCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATCg               <  1:10638622/140‑1 (MQ=255)

                                                                                                                         tAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATCg               <  1:1877208/140‑1 (MQ=255)

                                                                                                                          aaTCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCa                  >  2:4498291/1‑136 (MQ=255)

                                                                                                                          aaTCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATCGa              >  1:4933118/1‑140 (MQ=255)

                                                                                                                           aTCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATCGat             <  2:8125354/140‑1 (MQ=255)

                                                                                                                             cA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATCGatat           <  1:8166651/140‑1 (MQ=255)

                                                                                                                                 ttttttGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCtt        >  1:1649679/1‑140 (MQ=255)

                                                                                                                                   ttttGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTa      <  1:4334518/140‑1 (MQ=255)

                                                                                                                                   ttttGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTa      <  2:1979191/140‑1 (MQ=255)

                                                                                                                                      tGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTc                                                                         >  2:1044398/1‑70 (MQ=255)

                                                                                                                                      tGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCTCCGATGGCGGCTATTTCGGTGGTTTATTTCAATTTGGCGGCATCGATATCTTTATTc   <  1:5660358/140‑1 (MQ=255)

TGTTTTTAAATGTGATAAACCTTATACCTTGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCTGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCG  >  NZ_CP025268/1236525‑1236798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`